BaYMV resistance genes,
rym5 and
rym3, were pyramided using esterase isozyme genes (
Est1-Est2-Est4), whose loci had been reported to be tightly linked with
rym5. BaYMV reaction was also evaluated in the field contaminated with BaYMV strain I (to which cultivars with
rym5 and
rym3 are resistant) or III (to which cultivars with
rym5 is susceptible). Malting barley cultivars having
rym5 with esterase isozyme pattern
Ca-null-Nz (Mokusekko-type) were crossed with the cultivars having
rym3 with
Pr-Fr-Su (Prior-type). Selection of resistant lines in the field contaminated with BaYMV I with the esterase isozyme genotype of
Pr-Fr-Su (Prior-type) gave lines with
rym3. Selection of resistant lines in the field contaminated with BaYMV III with esterase isozyme genotype of
Ca-null-Nz (Mokusekko-type) gave lines pyramided with
rym5 and
rym3, in which the crossing-over rate was about 4.9%. During the selection by agronomic characters, the frequency of
rym3 was lowered significantly.
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