Japanese Journal of Crop Science
Online ISSN : 1349-0990
Print ISSN : 0011-1848
ISSN-L : 0011-1848
Volume 57, Issue 3
Displaying 1-21 of 21 articles from this issue
  • Yukio KUJIRA
    1988 Volume 57 Issue 3 Pages 411-417
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
    JOURNAL FREE ACCESS
    Varietal differences in the total nitrogen content of the roots or rice plants grown under 2 nitrogen dressing regimes (low fertilizer : 2.5 kg-N/10 a, high fertilizer : 7.5 kg/10 a) were investigated in 1984 by using the monolith method. Varietal differences in the decrease of the total nitrogen content of the roots and leaf blades as growth advanced were not recognized. The change in the amount of total nitrogen in the roots varied with cultivars. The cultivars used in this experiment can be divided into four types according to their patterns of change in the amount of total nitrogen in the roots, namely; a) those which showed no marked change in the amount of total nitrogen regardless of fertilizer levels and of growth stage, like "Sen-ichi" and "Norin 3" (A type), b) those which showed a significant decrease in the amount of total nitrogen with the increase in the amount of fertilizer dressed, like "Jyukkoku", "Asahi" and "Futaba" (B type), c) those which showed a proportionate increase with the amount of fertilizer dressed, like "Takenari" (C type), and d) those which showed variable response, where in the amount of total nitrogen was higher in the low fertilizer plot than in the high fertilizer plot in the early growth stage (July to August) and vice versa in the later stages (August to September) (D type). The general result of this study tends to suggest that there is a close significant correlationship between the amount of total nitrogen in the roots and root dry weight throughout the growth period of rice. Cultivars of A, B, and C types are distinct from each other on the regression line.
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  • Kin-ichi NISIKAWA, Naomichi TANAKA, Hirotoshi NAKANO, Hiroshi SAKAMOTO ...
    1988 Volume 57 Issue 3 Pages 418-423
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
    JOURNAL FREE ACCESS
    Diurnal changes in the activity of p-amylase and its isozyme patterns were examined in the seedlings of three-types of alfalfa (Medicago sativa L.) belonging to cultivars of three different types, i.e., extremely non hardy, intermediate and extremely hardy types. The results obtained are summarized as follows: 1. In all cultivars the p-amylase activity exhibited an alternating increase and decrease at every 3 to 6 hours interval. However, the tissue in which the highest and lowest p-amylase activity occurred in a day differed in cultivars, indicating varietal characteristics. 2. Within the β-amylase isozymes in alfalfa, the shoot tissues had the mainband A-5. Sub-band A-6 became detectable at the growth stage when the total activity was high. The total p-amylase activity and its isozyme banding patterns showed the same tendency, which made it possible to recognize varietal characteristics by means of β-amylase isozymes. 3. The p-amylase activity was higher in the stem tissues than in the leaves. This tendency was most remarkable in the extremely non-hardy type, but decreased gradually in the intermediate type and in the extremely hardy type. Although it is yet to be clarified, this tissue-specific differences in the p-amylase activity might reflect characteristics of cultivars.
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  • Koji ITO, Yoshio MURATA, Shinobu INANAGA, Tadakatu OHKUBO, Tomosiro TA ...
    1988 Volume 57 Issue 3 Pages 424-430
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
    JOURNAL FREE ACCESS
    Productivity of napiergrass (Pennisetum purpureum Schumach cv. Merkeron), in terms of annual yield (DMY), crop growth rate (CGR) and efficiency of solar energy utilization (Eu) for herbage dry matter in the first year under a heavy nitrogen supply, was compared among Tokyo, Nagoya, Fukuoka, Miyazaki, Naha and Iriomote. In the plants, grown without cutting during the growing season, the values of DMY, CGR and Eu were generally higher than those in most of the other crops at each site, and the regional differences in DMY and CGR showed no definite relationship with the regional differences in the levels of climatic factors, although the higher the solar energy input, the lower the Eu value. However, the productivity of the second growth after a cutting in August, was lower in the northern regions, due to lower temperatures.
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  • Koji ITO, Shinobu INANAGA
    1988 Volume 57 Issue 3 Pages 431-437
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    Napiergrass (Pennisetum purpureum Schumach, cv. Merkeron), grown in Tokyo and Miyazaki under relatively heavy fertilization, produced 31∼32 unfolded leaves on a stem, 20 leaves of which were lost by senescence, during the growing season from May to November. At both sites, the area of a leaf was the largest for the 23rd leaf on a stem, numbered consecutively from the lowest leaf above ground level. The area of this leaf, unfolded in late August, was ca. 370 cm2 in Tokyo and ca. 300 cm2 in Miyazaki, although the areas of 11∼13th leaves, unfolded in early July, were considerably smaller at the former site. Owing mainly to such circumstances as above, the increase in leaf area index and leaf area density until early September were more rapid in Tokyo than in Miyazaki. At both sites, the photosynthetic rate of single leaf under high light intensity was the highest for the leaves around the uppermost unfolded leaf with a value of ca. 90 mg CO2/dm2/hr and light-photosynthesis relations of these leaves were the ordinary type for C4-grasses. The older the leaf, the lower was the photosynthetic capacity and the more the light-photosynthesis curve tended towards the light-saturation type.
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  • Shigenori MORITA, Tetsuya SUGA, Koou YAMAZAKI
    1988 Volume 57 Issue 3 Pages 438-443
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    The relationship between mass or distribution of rice roots and yield were studied. Materials were rice plants cultivated in an experimental paddy field under various conditions in relation to the amount of nitrogen fertilizer and planting density. Soil cores were taken at the site below and between hills at a 30cm depth with a metallic cylinder (diameter : 15cm, length : 40cm) . After the roots were washed out using water on a sieve, the length of all the roots were measured with the Comair root length scanner made by Commonwealth Aircraft Corp., Ltd. From these data the root length density at each site and depth was calculated. With these root length densities the total root length per unit area (depth: 30cm) was estimated. The total root length increased with yield up to about 550g/m2 but not very much over 600g/m2. Then the distribution of roots were analyzed using the root length density. At the yield level over about 600g/m2 the proportion of deeper distributed roots was higher. These results indicate that the deeper distribution of roots was important for high rice yield.
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  • Masami KATSUTA, Yasuo OTA
    1988 Volume 57 Issue 3 Pages 444-448
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    Ethylene is known as a plant hormone which induces many morphological and physiological effects. The recent increase of ethylene concentration in the air as an air pollutant is causing unexpected defoliation or other undesirable phenomena in plants. Experiments were conducted to evaluate the effect of this compound as an air pollutant on rice plants, especially on its growth in the reproductive stage and on yield. Rice plants were fumigated by ethylene contained air, controlled at 0.1 ppm by flow meter, for two weeks. The fumigation started on 10 days (-10 days) and 4 days (-4 days) before heading, 2 days (+2 days) and 11 days (+11 days) after heading in 1983, and -10 days and -6 days, just heading day, and +7 days in 1984. In both years, fumigation started at -10 days showed a distinct morphological effect. The culm length was shortened, and the heading date was delayed about a week. The earlier the application was made, the flag leaves of every fumigated plant tend to show epinasty, and the treatment affected differentiation of spikelets, ripening rate and 1, 000 grain weight of plants. Consequently the grain yield decreased by about 20% in 1984. On the other hand, ethylene fumigation after heading stage accerelated the yellowing of panicles. This suggests that the senescence of plants were stimulated and the ripening stage period was shortened. The germination rate or the germination vigor of the seeds that were harvested from the fumigated plants, were not affected by the treatment.
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  • Tomoshiro TAKEDA
    1988 Volume 57 Issue 3 Pages 449-463
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    The numbers of genera and species of the subfamily Bambusoideae known, described and generally accepted by authors today were examined bibliographically and listed in the tables. The results was that the numbers of genera and species were 109 and 1056 respectively. Within the total number of genera and species of the subfamily Bambusoideae, the woody bamboos have a greater number of genera (77%) and species (84%) than the herbaceous bamboos. Bambusoid grasses are distributed in all the continents except Europe. Inside the world's tropical and subtropical belt bamboo is most densely distributed in eastern Asia and Central and South America. Africa causes by far a small number of species than America and Asia. The centers of diversity of bambusoid grasses seem to be the coastal regions of the Atlantic side of South America and the monsoon-belt of South-East and East Asia. Bambusoid grasses, though not a C4 subfamily, have an analogous tropical and subtropical distribution patterns which are broadly similar to the C4 grasses. Such difference as do exist are due to marginal differences in ecological adaptability, especially in damp and shady environments. These characteristics of the bambusoid grasses cause a reduction of C4% in the range of tropical and subtropical Rain-Forest and Monsoon-Forest districts.
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  • Makoto NAKATANI, Michitaka KOMEICHI
    1988 Volume 57 Issue 3 Pages 464-469
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
    JOURNAL FREE ACCESS
    We investigated the effect of holding of cut-sprouts for 5-10 days on the rooting under low soil temperature or moisture in sweet potato using cv. Koganesengan. The holding treatments were carried out under 16°C, 85% relative humidity and a weak light condition. The soil temperature was controlled at 20°C and 30°C. The soil moisture was adjusted to 30% and 70% of field capacity. Under the favorable conditions (30°C soil temperature or 70% soil moisture) the number of roots was decreased by the holding. However, the elongation of roots was accelerated and the total length of roots was increased about 10% by the holding. The rooting of cut-sprouts was restricted by the low soil temperature or moisture. The degree of restriction was smaller in the held cut-sprouts than in the non-held ones. At the low soil temperature the holding increased the number of roots by about 30%, the total length about 3 times, and the average root length and root dry weight about 2 times. Under low soil moisture the number of roots, the total length of roots, the average root length and root dry weight were also increased by holding about 50%, 3 times, 2 times and 2 times, respectively.
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  • Sachio MARUYAMA, Nobuyuki KABAKI, Koichi TAJIMA
    1988 Volume 57 Issue 3 Pages 470-475
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    Experiments on the growth response to nitrogen were conducted in 41 (1981) and 51 (1982) rice varieties including japonica, indica and japonica-indica hybrid. The ratio of the number of spikelet to straw weight of japonica-indica hybrid and semidwarf indica was larger than that of japonica. However, the ratio decreased greatly as a result of nitrogen application in these groups, although it did not change in japonica. The rate of increase in dry weight of the leaf blade due to nitrogen application was larger than that in dry weight of the culm and leaf sheath, and the ratio of the rate of increase in dry weight of the leaf blade to that in the number of spikelet was larger in japonica-indica hybrid and semidwarf indica than in japonica. Coefficient of variation of the number of spikelet in a hill of japonica-indica hybrid and semidwarf indica was larger than in japonica, and several varieties of these groups with a large mean number of spikelet per panicle had a large percentage of the number of spikelet on the secondary and tertiary rachis branches. Japonica-indica hybrid and semidwarf indica, which were thought to have high-yielding characteristics, had a large ratio of spikelet number to straw weight, but, this advantageous characteristic decreased when nitrogen was applied. Moreover, these groups also had disadvantageous characteristics for ripening. It was concluded that these characteristics should be improved for breeding high-yielding varieties by using japonica-indica hybrid or semidwarf indica as genetic resources.
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  • Tomomi NAKAMOTO, Koou YAMAZAKI
    1988 Volume 57 Issue 3 Pages 476-481
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    Morphological characters of successive shoot-units on a main shoot (Fig. 1, 2) were observed in field grown millets, i.e. Setaria italica, Sorghum bicolor Pennisetum typhoideum, Panicum miliaceum, Echinochloa frumentacea, Eleusine coracana, Coix lacryma-jobi and Coix lacryma-jobi var. frumentacea. All species showed a linear, but species-specific relationship between the number of shoot-units with emerging leaf and those with emerging primary roots (Table 1). At maturing, the length and width of the leaf blade, length of leaf sheath, length of internode and the diameter of the stem increased acropetally, reached the maximum, then decreased (Fig. 3). The length of leaf sheath, length of internode, length of leaf blade and width of leaf blade reached the maximum in the order as stated above. The number and diameter of the primary roots increased acropetally. In all species examined, the shoot-units on the main stems can be divided into three shoot-unit groups (two groups in Eleusine coracana), i.e. 1st, 2nd and 3rd groups respectively, according to the acropetal trends in morphological characters, especially the length of internodes (Fig. 3). According to these trends, eight species can be ranked in a sequence. The bearing of the results on the mechanisms causing such morphological trends were discussed with regard to the developmental processes of each organ.
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  • Tomomi NAKAMOTO, Koou YAMAZAKI
    1988 Volume 57 Issue 3 Pages 482-489
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    The number, size and morphology of vascular bundles were observed in transverse section at the base of leaf blades and at the center of leaf sheaths. The results were fundamentally the same in seven species of millets examined. The number and size of both large and small vascular bundles increased as the leaf position (shoot-unit) became higher (Fig. 2, 3). In the large vascular bundles, cross sectional area of phloem, cross sectional area of xylem and diameter of late metaxylem vessel were proportional to the size of the bundle (Table 1, 2, 3). But in the small vascular bundles, the cross sectional area of xylem was not in proportion to the size of the bundle, and smaller small vascular bundles had relatively smaller amount of xylem (Table 2). The total cross sectional area of phloem at the base of a leaf blade was larger than that at the center of leaf sheath. But the total cross sectional area of xylem in the leaf blade was smaller than that in the leaf sheath (Fig. 4). Large leaves had large total cross sectional areas of phloem and xylem, but the total cross sectional areas of phloem and xylem per unit leaf area were smaller than that of small leaves. The determining process and functional meaning of a quantitative relationship between the amount of conducting tissues and leaf were discussed.
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  • Tomomi NAKAMOTO, Koou YAMAZAKI
    1988 Volume 57 Issue 3 Pages 490-495
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    Conducting tissues at the base of primary roots in seven millets were observed. 1. Primary roots with a large diameter showed high values of < diameter of stele/diameter of root > (Table 1). The proportion of the cross sectional area of stele to that of root was high in Sorghum bicolor and Pennisetum ryphoideum, and was low in Echinochloa frumentacea and Coix lacryma-jobi (Fig. 2). 2. In every species examined, the number of both phloem pole and pericycle cells were proportional to the diameter of the stele (Table 3, 4). 3. The number and mean diameter of late metaxylem vessels were not always proportional to the diameter of the stele (Fig. 4, 5). The primary roots with a large diameter often had relatively few and small vessels as compared with the root diameter.
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  • Yusuke GOTO, Kiyochika HOSHIKAWA
    1988 Volume 57 Issue 3 Pages 496-504
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    In rice plants, the leaf number of the main stem is used to express the plant age. But there was no way to express the age of the individual tiller. We used the concept of plant age indicated by leaf number to estimate the age of the individual tillers. The age of individual tillers expressed by leaf number is the number of spread leaves plus the ratio of the length of the emerged part of the leaf-blade. To compare the ages of every tiller with each other, we prescribed a relative tiller age (RA) to the individual tillers. Using these indicators, we could compare the growth of tillers with that of the main stem. Plants of three rice cultivars were in pots (1 plant/pot) under irrigated conditions, and the position of each leaf was marked. The leaf emergence rate of early developed tillers changed simultaneously with that of the main stem. During the late vegetative stage, the leaves of higher leaf position on tiller emerged at the higher order of tillers. The differences (D) in RA between tillers and the main stem increased with the plants' growth, and peaked just before the flag leaf emergence. The tillers of higher order showed a larger D, and there was a varietal difference in the value of D.
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  • Sachio NISHIBE, Kazuto IWAMA, Kimio NAKASEKO
    1988 Volume 57 Issue 3 Pages 505-511
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
    JOURNAL FREE ACCESS
    The tuber starch weight in three varieties of potato plant was observed 5-8 times during the growing season (June to September) for ten years (1972-1981) at the Hokkaido National Agricultural Experiment Station. 1. The starch yield in each variety showed a significant correlation with the growth rate of the tuber starch weight (tuber GR), and not with the bulking period (Table 1). 2. At the early bulking stage, high solar radiation, little precipitation and a maximum temperature between 22-23°C enhanced the tuber growth, but restricted the shoot growth, resulting in a low shoot fresh weight at the main bulking stage, especially in the early variety (Figs. 1 and 2). The meteorological factors accounted for 68% of the variance in the distribution ratio for the tuber on a fresh weight basis. 3. At the main bulking stage, the tuber GR showed a significant correlation with the shoot fresh weight in the early variety, but with the shoot efficiency (tuber GR/shoot fresh weight) in the late varieties. The shoot efficiency was higher under the conditions of high solar radiation, little precipitation and a minimum temperature of about 15°C (Table 2, Fig. 3). The meteorological factors accounted for 54% of the variance in the shoot efficiency (Fig. 4). 4. At the late bulking stage, the tuber GR showed a significant correlation with the shoot fresh weight in the late varieties.
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  • Teruhisa UMEZAKI, Shigeo MATSUMOTO, Itaru SHIMANO
    1988 Volume 57 Issue 3 Pages 512-521
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    To demonstrate the advantages of dwarf characters in soybean, the Hyuga-dwarf line, a dwarf mutant induced from cultivar Hyuga by the application of ethyleneimine in 1975 was compared with Hyuga. The results obtained are summarized as follows: 1. Hyuga-dwarf line shows a slower vegetative growth rate than Hyuga in the quantitative characters such as main stem length and stem weight, whereas no significant differences have been detected between them in the qualitative characters such as total node number. 2. Hyuga-dwarf line has several advantageous characters in dry matter production and distribution, such as prolongation of leaf longevity, thickening of lamina and a high seed-stem weight ratio, when compared with Hyuga. 3. The dwarf character coded by a pair of gene is recessive to normal one. It is suggested that this gene expresses its character only at the top. In conclusion these results indicate that Hyuga-dwarf line has advantageous Harvest Index and Dry Matter Distribution Rate than Hyuga. It is suggested that productivity of the Hyuga-dwarf line higher than Hyuga will be obtained by improved cultivation methods, and further, that this gene will be a useful genetic resource for soybean improvement.
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  • Tadashi TAKAHASHI, Kimio NAKASEKO, Kanji GOTOH
    1988 Volume 57 Issue 3 Pages 522-526
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    Dry matter accumulation of each part on a main shoot and its partitionings (Δparts/Δshoot, %) were measured at 3 to 7 days intervals during the growing season using the field grown (400 pls./m2) spring wheats, tall Haruhikari and semi-dwarf 2-47. The seasonal pattern of dry matter partitioning of Haruhikari and 2-47 was almost identical (Fig. 2). Based on the pattern, the growth was divided into the following five main phases (Fig. 3). Growth phase I (emergence - flag leaf initiation); dry matter is mainly partitioned to the leaf blade (60-80%). Growth phase II (initiation - emergence of flag leaf); after the start of elongation of the leaf sheath and the culm, the partitioning to the leaf blade decreases and almost ceases during this phase. Growth phase III (flag leaf emergence - anthesis); dry matter is partitioned to the culm and the ear (without grain). The partitioning to the ear almost ceases in this phase. The partitioning to the cellular contents in the culm decreases temporarily at about the time of heading. Growth phase IV (anthesis - milk ripe stage); the partitioning to the grain starts to increase about a week after anthesis. The partitioning to the culm decreases rapidly but that to the cellular contents still continues. Growth phase V (milk ripe stage - maturity); all of the dry matter including reserve material is partitioned to the grain. In 2-47 the partitioning to the culm during the growth phase II was lower, and to the ear during the growth phase III and to the grain during the growth phase IV were higher than those in Haruhikari.
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  • Shuichi MIYAGAWA, Toshiro KURODA
    1988 Volume 57 Issue 3 Pages 527-534
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    Rice production in Northeast Thailand tends to show both low yield per unit land and unstable crops. The objective of this report is to clarify the actual situation of the rice cultivation. One typical rice growing village, Don Daeng which is located l5 km from Khon Kaen city was chosen for the study. A survery of growth of rice, yield and its components was carried out in the village. Thirty one and 30patchs were selected as sample paddy fields in 1981 and 1983, respectively. Rice cultivation of this village depends upon traditional local varieties and rain-fed paddy fields without fertilizer. In 1981, unhusked grain yield was so low as the mean value of 181.6g/m2 due to drought. The yield showed a positive correlation with the each component. On the other hand, in 1983 the yield increased to 236.3g/m2 as the mean value due to enough rain fall, and showed a correlation with the number of panicles per square meter. The growth and yield tremendously fluctuated among paddy fields in both years. The yield correlated positively with the straw weight and the leaf area index, and negatively with the light transmission rate under a plant canopy. Moreover, concentrations of nitrogen, phosphorus and potassium were remarkably low. These results seem to suggest that increases in leaf area index and the number of spikelets per unit area by vigorous vegetative growth lead to a yield increase.
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  • Makoto NAKATANI, Atsushi OYANAGI, Yasushi WATANABE
    1988 Volume 57 Issue 3 Pages 535-543
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    An attempt was made to clarify the relationship between the formation and growth of tubers and the photosynthetic rate during the early growth stage in sweet potato by using the grafts of several cultivars differing in sink potential. The tuber formation largely depended on the characteristics of stock cultivar. Both of the stock and scion effects on tuber thickening were observed at the stage after its formation. A positive correlation with tuber dry weight was found in the dry weight of petioles, leaf blades or leaf area, and a negative one was observed in the ratio of number of dead leaves to total leaves. There was no relation between the photosynthetic rate and the root dry weight at the stage before tuber formation. However the photosynthetic rate in a scion correlated with tuber dry weight at the stage after the tuber dry weight amounted to about 10g/plant. Thus the results suggested that the tuber becomes dominant sink of assimilate and the source activity was influenced by tuber sink activity quantitatively after this stage.
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  • Makoto NAKATANI, Michitaka KOMEICHI, Yasushi WATANABE
    1988 Volume 57 Issue 3 Pages 544-552
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    An estimation of the tuber sink potential of several sweet potato cultivars was attempted. For this purpose we used stocks of single rooted leaves of several cultivars grafted with uniform single leaf scions of a single type of cultivar, because the source potentials i.e. leaf area and photosynthetic potential were able to be uniform and sink organs other than tuber were restricted. The source activity, i.e. leaf area and apparent photosynthetic rate, did not differ statistically among stock cultivars. However, the tuber dry weight correlated significantly with the leaf area, therefore the tuber sink potential was estimated by the tuber dry weight per unit leaf area. The apparent photosynthetic rate was regulated by mesophyll resistance of CO2 diffusion, and the mesophyll resistance was influenced by tuber sink potential through the control of leaf starch content. Thus, the matter production of grafts was largely depended on the tuber sink potential in this system. The tuber sink potential was different among stock cultivars significantly at 1%. It was higher in cv. Koganesengan and Shirosatsuma and lower in cv. Tsurusengan.
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  • Kwan-Long LAI, Li-Fei LIU
    1988 Volume 57 Issue 3 Pages 553-557
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    Plant regeneration ability in cells of rice (Oryza sativa L.) was closely correlated with its water status. The water content of the highly regenerating callus of ANT 39 was about 10% less than that of the poorly regenerating callus of TN 5. Plant regeneration frequency of TN 5 callus was apparently increased when its water content was reduced by water stress treatment. Mannitol and higher concentration of agar were used as a water stress inducing agent. Assessment of changes in the proline content of callus suggested the effect of water stress.
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  • Said Hassan RADI, Eizo MAEDA
    1988 Volume 57 Issue 3 Pages 558-564
    Published: September 05, 1988
    Released on J-STAGE: February 14, 2008
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    The excised rice roots with attached scutellum were cultured simultaneously utilizing two separate media. The root base was supplied with 12.5% sucrose via scutellum, while the root tip was immersed in the inorganic nutrients of MS medium diluted 4 times. Both root and scutellum media were supplied with different concentrations of ABA and/or figaron. ABA supplied to the scutellum medium stimulated root growth only at the relatively low concentrations, while the high concentrations were inhibitory. On the other hand supplementation of ABA to the root medium drastically inhibited root growth. Although the primary root length was detectably stimulated when ABA 10-9 M was supplied to the scutellum medium, it was drastically inhibited in the case of decapitated roots cultured under the same conditions, but the fresh weight and lateral branches were detectably increased as compared with control. Also the effect of a mixture of figaron and ABA supplied to the scutellum medium (in the same concentrations which stimulated root growth when applied separately) was found to be drastically inhibitory for root growth. Furthermore the simultaneous application of figaron and ABA to scutellum and root media respectively inhibited root growth.
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