There is a field in Fujimi, Saitama, Japan (unfertilized; UF) where an ordinary amount of crop is produced for marketing without applying any fertilizer or returning above-ground crop residues. We aimed to clarify the source of N in the leaves of tomato cultivated in this field, using the δ
15N method. In an adjacent conventional single cropping tomato field (conventionally fertilized; CF), chemical fertilizer and compost (100 and 45 g N m
-2, and δ
15N of -1.7 and +9.3‰, respectively) had been applied as basal fertilizers. In UF, the δ
15N values of soils were +7.2‰ in the 0 - 20 cm surface soil and +7.1‰ in the 20 - 35 cm deep subsoil; the corresponding values in CF were +8.5 and +7.5‰, respectively. Compared with those soils, δ
15N values in tomato leaves were much lower, around +3‰ in both UF (+ 3.2 ± 0.4‰) and CF (+ 3.0 ± 1.0‰) fields. These values in tomato in CF, it may be explained by dilution with absorbed chemical fertilizers; but those in UF, without artificial input, cannot be explained by the absorption of the soil N, which has relatively high δ
15N values. The results obtained from the UF field suggest that the field had received a substantial amount of N with relatively low δ
15N values like atmospheric N from the outside of the field-plant system and that it allowed the ordinary crop production in the UF field.
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