Sustainable agriculture, which reduces environmental burden, has become increasingly important in recent years. C
4 plants are considered to be useful plant resources as both food and energy crops, because they show high dry matter productivity with high water and nitrogen use efficiencies. However, the studies on the effective use of C
4 plants is not sufficient. In fact, the basic knowledge is scarce on many C
4 plants except for a few crops. The C
4 plants occurring in Japan are listed in this study, as a first step to utilize C
4 plants including weeds. There are 419 C
4 plant species belonging to 91 genera in 11 families, identified in Japan. They are comprised of 62 eudicot species belonging to 19 genera in 8 families, and 357 monocot species belonging to 72 genera in 3 families. Compared to the checklist in 1990, 19 eudicot species and 157 monocot species have been added to the list. C
4 eudicots belonging to Aizoaceae, Asteraceae and Cleomaceae have newly been identified. However, C
4 eudicots belonging to Acanthaceae, Boraginaceae, Caryophyllaceae, Molluginaceae and Scrophulariaceae have not been found in Japan. Monocot submerged aquatic plant, Hydrilla verticillata in Hydrocharitaceae has been found to induce C
4 photosynthetic metabolism under conditions of low CO
2 concentration in water. This finding has increased the family number of C
4 monocots to 3. The list has 6 C
3-C
4 intermediate species, 5 of which have newly been identified as introduced plants. Lots of C
4 plants identified newly in Japan, more exactly, 84% of C
4 eudicots and 46.8% of C
4 monocots are the introduced plants including cultivated species. This indicates that C
4 plant seeds have contaminated imported raw materials, and invaded Japan in the same way as other introduced weed seeds.
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