Connected with the previous report, the present one deals with the seasonal changes of Ca, Mg, Fe and Mn contents within the main culm of rice plant, the pattern of their accumulation and translocation and the interrelations among those elements. The results obtained were as follows: 1) The Ca percentage was highest in L.B. than in other parts, and it increased continuously with leaf age on every position, that is, being high in the lower leaves and becoming low in the upper ones in proportion to their positions on the culm. The Ca in L.S. was much lower than that in L.B., though its behaviour was similar to that in L. B.. Ca was contained largely in L. B., it being highest in the 13th L.B.. In L.S. and I.N. Ca contents were small; in some of them it was recognized to have been translocated away. Though Ca was considered not to play a prominent role in grain ripening, Ca absorption by root was assumed to have continued till the yellow ripe stage at least, as Ca was found accumulated in the upper vegetative parts till full ripe. 2) The Mg percentage in L.S. and I.N. was nearly equal, being slightly higher in L.B.. especially that of middle leaves max. in the 13 or 14th L.B. Accumulation and translocation of Mg in L.B. as well as in L.S. occurred on and after their expansion. With regard to L.S., the largest amount of Mg was contained in middle leaves, too. Mg in I.N. was observed to be translocated into grain after heading time. The role of Mg in grain ripening process was as great as that of N or P. Hence the largest role in the grain ripening was assumed to be played by Mg in the middle parts. 3) Fe percentage was highest in the lower L.S., lower in the upper L.B. and L.S., and lowest in the hull and grain. Fe amount was highest in the 11-13th leaves. Accumulation and translocation of Fe were made in L.B., L.S. and I.N. respectively as the parts became aged. The Fe in L.S. and I.N. of the upper part was recognized to play a role in ripening. 4) Mn percentage was generally high in L.B., medium in L.S., and low in I.N.; higher in the lower leaves and lower in the upper ones. The behaviour of Mn in eachleaf was much the same as that of Ca. Mn amount was similar to Ca, but in L.S. the former was found to be much more than the latter. The role of Mn in ripening was assumed to be as little as that of Ca. 5) As a result of the consideration of the relation among K-Ca-Mg, the relations of Ca+Mg/K to Ca/Mg seemed to be antagonistic in all parts within the main culm. 6) The relation between P and Mg was also found to be interactive. 7) The Fe-Mn relation was also regarded to be similar to the Mg-Ca relation. 8) The general order of accumulation in all parts is assumed as follows: [P, N, Dry matter, K] [Mg, Fe] [Mn, Ca]. But especially in the upper I.N., the order changed as follows: K, Fe [N, P, Dry matter, Mg, Ca, Mn]. 9) Physiological functions of successive leaves and elements concerned with ripening were diagrammatized as follows:[table]
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