Kanzo

HCV-RNA of the hepatocytes of the complete responders with disappearance serum HCV-RNA after IFN treatment

Using in situ hybridization

Mitsuhiro TERADA, Yujiro TANAKA, Yosuo MATSUBARA, Toshiyuki BABA, Mits ...

1994 Volume 35 Issue 12 Pages 835-840
Published: December 25, 1994
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.35.835

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HCV-RNA in hepatocytes was examined using in situ hybridization (ISH) in 21 complete responders with serum HCV-RNA disappearance, after chronic hepatitis C IFN treatment. Of these patients, hepatocytes HCV-RNA was found in 42.9%. Patients whose hepatocytes HCV-RNA were disappeared did not relapse. However, 11.9% of patients with hepatocytes HCV-RNA remaining, after IFN treatment, showed recurrences of serum HCV-RNA and elevated ALT. These result suggested patients with disappearance of HCV-RNA in hepatocytes from 6 months after IFN treatment show very good prognosis, while those with HCV-RNA remaining must be carefully observed and may become carriers.

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The mechanism for elevation of plasma tissue plasminogen activator levels in patients with liver cirrhosis: Analysis of release response to endothelial cell stimulation during venous occlusion

Takeshi HAYASHI

1994 Volume 35 Issue 12 Pages 841-848
Published: December 25, 1994
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.35.841

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The mechanism for the development of elevated plasma tissue plasminogen activator (t-PA) levels in liver cirrhosis was investigated. Changes in plasma t-PA and plasminogen activator inhibitor 1 (PAI-1) levels were measured using a venous occlusion test. The plasma t-PA levels after venous occlusion were significantly higher than those before venous occlusion in the control, compensated liver cirrhosis (LCC) and decompensated liver cirrhosis (LCD) groups. The plasma PAI-1 levels did not change significantly in any group.
The venous occlusion test showed that the change in the t-PA value (Δt-PA) in the LCD group was significantly higher than that in the other groups. The change in the PAI-1 value (ΔPAI-1) did not differ significantly between groups. Among patients with liver cirrhosis, those with severe esophageal varices had significantly higher Δt-PA values than those without varices or those with mild varices.
These results suggest that the elevated plasma t-PA level in patients with liver cirrhosis may be related to an increased release of t-PA and not PAI-1 in response to endothelial cell stimulation.

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Significance of TNF-α, GM-CSF in serum on diagnosis of HCC and recurrence after operation

Haruhiro NAKAZAKI, Yasushi SUZUKI, Yasuhiro SHIMOJIMA, Shintaro KURAMO ...

1994 Volume 35 Issue 12 Pages 849-854
Published: December 25, 1994
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.35.849

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TNF-α, IL-1β, IL-2, IL-6, GM-CSF in serum of patients with HCC were measured before and after operation. We made a comparison between these cytokines with recurrence and without recurrence and investigated whether these cytokines are useful tumor markers of not. Serum levels of TFN-α, IL-1β, IL-2, IL-6, GM-CSF were high in patients with HCC before operation in comparison with those of normal controls. The levels of TNF-α, IL-1β, GM-CSF in patients without recurrence decreased and IL-6 increased. The levels of TNF-α, IL-1β, GM-CSF in patients with recurrence increased again. The levels of TNF-α and GM-CSF in cancer tissue were high, the levels of IL-2 and IL-6 in non-cancer tissue were high.
The levels of TNF-α, IL-6 and GM-CSF in supernatant of five HCC cell lines were high. These results suggest that TNF-α and GM-CSF in serum are important on diagnosis of HCC and recurrence after operation.

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Clinicopathological study on scirrhous hepatocellular carcinoma-A study of 12 resected cases-

Hayato IHA

1994 Volume 35 Issue 12 Pages 855-863
Published: December 25, 1994
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.35.855

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Clinicopathological features of 12 resected cases of scirrhous hepatocellular carcinoma (HCC) was studied and compared with those of 307 cases of non-scirrhous type of HCC as the control group.
In comparison with the control group, the prevalence of HBsAg positive was significantly higher in scirrhous-HCC (41.7%) than in the control group (18.2%). There was no significant difference in age, sex, pre-operative data of blood biochemistry and tumor markers. Although there was no significant difference in survival rate, the confirmed recurrence rates were 8.3% in scirrhous-HCC and 55.2% in the control group, respectively, with significant difference. Thus, it was assumed that the prognosis of scirrhous-HCC is better than that of common type of HCC.
Pathomorphological studies revealed that scirrhous-HCC often showed the feature of central fibrous scars (58.3%). Microscopically, scirrhous-HCC was characterized by the presence of fibrous connective tissue along the blood space. Some other characteristics features of scirrhous-HCC included abscence of capsule (100%), presence of portal tracts (75.0%) and varying degrees of lymphocytic infiltration (75.0%) within the tumor nodule. In addition, the scirrhous-HCCs were frequently observed in Mallory bodies and pale bodies with a statistical significance.

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Postinfantile giant cell hepatitis accompanied by hypereosinophilia

Hirohito MURAMATSU, Shuichi NOJIRI, Yasuhiro NAGAMACHI, Kazuhiko KOIKE ...

1994 Volume 35 Issue 12 Pages 864-869
Published: December 25, 1994
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.35.864

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A 70-year-old woman was refered to our hospital with complaints of fever and general malaise. Laboratory examinations on admission showed raised levels of AST (1, 546IU/l) and ALT (940IU/l) and marked eosinophilia (leukocyte count 9140/mm3 with 60% eosinophils. Liver biopsy on day six of admission revealed multinucleation and degeneration of hepatocytes with occasional ballooning, and massive infiltration of eosinophils. Second liver biopsy performed on the fifteenth hospital day showed a healing stage of acute hepatitis, although, some residual multinucleated hepatocytes and infiltration of eosinophils were still observed. Levels of AST and ALT, and count of eosinophilis gradually normalized over the 2 months. Despite extensive examinations, no specific causes of the disease was identified. The association of giant cell hepatitis and hypereosinophilia has been so far described only in one case. Although the mechanism of appearance of multinucleated hepatocytes has been unclear, an ultrastructural study of present case suggested fusions of membrane as a multinucleation of hepatocyte.

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Clinical effect of Cyclosporin A in therapy of severe hepatitis-analysis of 5 patients administered

Shuji WASAKI, Mitsuru YASUNAGA, Yasuhiro MATSUMURA, Atsuko NAGATOMI, K ...

1994 Volume 35 Issue 12 Pages 870-875
Published: December 25, 1994
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.35.870

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Cyclosporin (CsA) is known to be strong immunosuppressive agent and available for organ implantation. We studied the clinical effect of CsA on 5 patients of severe hepatitis. These were three of acute aggravation in chronic hepatitis type B, one of autoimmune hepatitis which progressed hepatic failure, and one of alcoholic hepatitis. Laboratory data revealed decreased prothrombin activities less than 40% and hyperbilirubinemia. Ultrasonography showed existence of ascites in all patients. Three of 5 patients to whom CsA had been administered from early stage of hepatic injury could survive. In these survived patients serum total bilirubin levels reduced and the other laboratory data normalized soon after administration of CsA. These results indicate that administration of CsA from early stage of severe hepatic injury might be useful in therapy of fatal liver damage.

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A case of primary biliary cirrhosis (PBC) followed with abdominal US, PTP and histological findings of the liver for five years-Evidence for the changes in intrahepatic portal vein tributaries-

Yoshihiko KAZAMA, Kazuhiko HOSHINO, Hiroyuki INABA, Makoto MIYAWAKI, H ...

1994 Volume 35 Issue 12 Pages 876-881
Published: December 25, 1994
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.35.876

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The authors report a case of PBC followed with abdominal US, PTP and histological examinations of the liver during five years.
A 62-year-old woman was diagnosed of asymptomatic PBC. Thereafter her conditions seemed to progress to liver cirrhosis because of portal hypertension such as ascites and varices. The histological findings of the liver, however, revealed no cirrhotic changes but portal fibrosis particularly around the portal vein branches with slight abnormalities in the liver parenchyma on autopsy.
At the same time PTP demonstrated remarkable changes on the peripheral branches of portal veins in the liver, inducing portal hypertension, as compared with the first time PTP. The present study would provide evidence that portal hypertension in PBC is caused mainly by the narrowings of intrahepatic portal vein branches in the precirrhosis stage.

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A case of acute carbon tetrachloride poisoning

Takao HOSHINO, Masafumi KOMATSU, Tsuyoshi ONO, Masato FUNAOKA, Junji K ...

1994 Volume 35 Issue 12 Pages 882-886
Published: December 25, 1994
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.35.882

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A 55-year-old man who had drunk for past twenty-five years accidentally ingested carbon tetrachloride. He was admitted to a local hospital, complaining of abdominal pain, nausea, vomiting and diarrhea 2 hours after ingestion. He was transferred to our hospital because his condition rapidly deteriorated. Examination on admission revealed mild jaundice and hepatomegaly. Laboratory findings were as follows; AST 5160 IU/l, ALT 3000 IU/l, T. Bil 2.5 mg/dl, PT 35.3%, superoxide dismutase (SOD) 36.2%. Haptoglobin and lipoperoxide were within normal limit. His liver function was normalized at twenty-eight days after admission. Histological findings were as follows; perivenular and pericellular fibrosis, balooned hepatocytes, infiltration of small round inflammatory cells and polymorphonuclear cells, fat droplets and necrosis of hepatocytes. These changes were dominant in centrilobular area (Zone 3). These findings were compatible to those of acute carbon tetrachloride poisoning superimposed on chronic alcoholic changes and may suggest that hepatotoxicity of carbon tetrachlo-ride is reinforced by alcohol intake.

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[title in Japanese]

[in Japanese]

1994 Volume 35 Issue 12 Pages 887-891
Published: December 25, 1994
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.35.887

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1994 Volume 35 Issue 12 Pages 892-893
Published: December 25, 1994
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.35.892

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

1994 Volume 35 Issue 12 Pages 894
Published: December 25, 1994
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.35.894

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