Kanzo
Online ISSN : 1881-3593
Print ISSN : 0451-4203
ISSN-L : 0451-4203
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Volume 28, Issue 11
Displaying 1-21 of 21 articles from this issue
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  • Clinical study for the prevention of HB virus infection
    Akira YOSHIKAWA
    1987 Volume 28 Issue 11 Pages 1413-1422
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1413
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    In order to prevent HB virus infection, 407 persons of medical staff who received accidental HBV exposure such as needle stick, blood on cut, suture needle stick and so on, were adminiStered HB globulin (HBIG). Two of them merely developed acute hepatitis (type B). Thereafter, we administered HB-vaccine three times againSt 596 persons with neither HBsAg nor anti-HBs. Out of them, 533 persons (89.4%) acquired anti-HBs. Moreover, we carried out the study of prevention for maternal transmission of HBV by combined use of HB vaccine and/or HBIG. Compared that 57 out of 67 infants (85.1%) born to HBeAg pOsitive carrier mothers became HBV carrier without treatment, 4 out of 75 infants (5.3%) merely became HBV carrier.
    These results indicated that HBIG and HBvaccine administration was extremely effective for the prevention of accidental and perinatal transmission of HBV.
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  • The molecular form of IgM antibody to hepatitis B core antigen in sera from patients with hepatitis B virus infection
    Mitsuo SUGA, Katsuji YOKOTA, Toshinori KODAMA, Toshimitsu HIRANE, Yosh ...
    1987 Volume 28 Issue 11 Pages 1423-1427
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1423
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    The molecular form of IgM antibody to hepatitis B core antigen (IgM anti-HBc) was analysed by high speed liqud chromatography and enzyme immunoassay (CORZYME-M) in 8 sera from 6 patients with acute hepatitis B virus infection and in 6 sera from 6 patients with chronic hepatitis B virus infection. The IgM anti-HBc was detected in not only 19S IgM fractions but also 7S IgM fractions. The IgM anti-HBc of 19S IgM fractions, however, was more predominant than that of 7S IgM fractions in both patients with acute and chronic hepatitis B virus infection. The result suggests that the molecular form of IgM anti-HBc may not be useful for differentiating acute from chronic hepatitis B virus infection.
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  • Clinical and histological studies on formation of lymphoid follicles in various liver diseases
    Haruhiro NISHIKAWA
    1987 Volume 28 Issue 11 Pages 1428-1438
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1428
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    To investigate the significance and the role of lymphoid follicles in the liver, clinical, histopathological and histochemical studies were performed. Three thousand cases that underwent liver biopsy were examined and lymphoid follicles were observed in 495 cases. Follicle-rich 141 cases (95 males, 46 females) were extracted for detail survey. In these cases, 131 cases (93%) were HBsAg negative. Histologically, lymphoid follicles were observed mostly in the liver of the cases with chronic hepatitis (not only chronic active hepatitis but also chronic inactive hepatitis) and nonspecific reactive hepatitis. They were sometimes ovserved in the liver diseases based on cholelithiasis, diabetes mellitus and autoimmune disease. Clinically, the cases with lymphoid follicles were characterized by hypergammaglobulinemia and slight abnormality of transaminase and had better prognosis than without lymphoid follicle. The T/B lymphocytes distribution in the follicles of the liver was similar to that of the lymphonodi.
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  • Cytochemical demonstration of guanase in human liver using yellow tetrazolium
    Susumu ITO, Jyoji SYUNDO, Akihiko ISHIHARA, Naoyuki KITAGAWA, Yasuhiro ...
    1987 Volume 28 Issue 11 Pages 1439-1443
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1439
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    We reported a modified procedure for histochemical demonstration of guanase in human tissues involving hydrolytic deamination of the substrate guanine to xanthine via guanase, and then oxidation of xanthine to uric acid, with concomitant reduction of nitro tetrazolium blue (NBT). In this report, we describe a modification of this method for cytochemical demonstration of guanase at the fine structural level using yellow tetrazolium in place of NBT for determination of the intracellular distribution of guanase in human hepatocytes.
    In the hepatocytes, the reaction products were seen to be concentrated in the nucleus, in mitochondria, cisternae of the smooth and/or rough-surfaced endoplasmic reticulum and lysosomes. The precise locations of the reaction product in the cisternae of the nuclear envelope, chromosomes and nucleus could not be determined. However, the reaction products in the mitochondria were clearly seen to be located in the spaces of cristae.
    This information of the intracellular distribution of guanase in normal hepatocytes will be useful in determing the physiological role of this enzyme and in further studies on diseased hepatocytes including those in non-A non-B hepatitis.
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  • Clinical significance of lysosomal copper and iron in human hepatocytes
    Atsushi SASA, Akira YAGI, Hisao HAYASHI, Nobuo SAKAMOTO
    1987 Volume 28 Issue 11 Pages 1444-1450
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1444
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    Subcellular distribution of trace elements was investigated in the hepatocytes of 33 liver disease patients. Ultrathin frozen sections of glutaraldehyde-fixed liver specimens were mounted on golden mesh without staining. These were examined for copper, iron and sulfur with an electron microscope equipped with an energy dispersive x-ray microanalyzer. The three elements were distributed predominantly in the lysosomes. Copper was detected in 30 of 33 specimens; iron in 16 specimens. Both metals were always associated with sulfur. There was a correlation between copper/phosphorus and sulfur/phosphorus (Y=1.31X+0.66, r=0.88), but not between iron/phosphorus and sulfur/phosphorus. The estimated molar ratio of copper to sulfur was consistent with that of metallothionein.
    The copper-thiol complex is a result of impaired biliary secretion of copper in the cases of Wilson's disease and primary biliary cirrhosis and probably participate in detoxication of the metal. It must be emphasized that the same complex was found in most patients with fatty liver or chronic hepatitis. This means that both fatty liver and chronic hepatitis may be associated with partial impairment of biliary secretion.
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  • Immunoelectron microscopic study of IgA deposition in livers of patients with chronic liver diseases
    Yoshikazu TANAKA
    1987 Volume 28 Issue 11 Pages 1451-1459
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1451
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    In order to clarify the significance of IgA of patients with chronic liver disease, the IgA deposition along the hepatic sinusoids was studied by immunoelectron microscopy. By light microscopy, two diferent staining patterns of IgA were recognized: linear and granular. The former was pathognomonic to alcoholics, and the latter was frequently observed with chronic agressive hepatitis.
    By immunoelectron microscopy, the linear pattern corresponded to the diffuse reaction deposition in the cytoplasmic matrix of sinusoidal endothelial cells, and the granular pattern corresponded to the round granular reaction products in the same cells.
    The double staining method revealed that the localization of IgA is different from lysozyme or endogenous peroxidase activity.
    These results suggest in the chronic liver cell damage, IgA and reticulo-endothelial system are closely connected.
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  • Quantitation of the intrahepatic shunt index by injection of 99mTc-macroaggregated albumin into the spleen
    Masaaki SAITO, Kunihiko OHNISHI, Hiroshi KATSURAI, Hideo TANAKA, Nobuy ...
    1987 Volume 28 Issue 11 Pages 1460-1466
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1460
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    The accuracy of the intrahepatic shunt index measured by injection of 99mTc-macroaggregated albumin into the spleen was assessed in comparison with the intrahepatic shunt index measured by injection of 99mc-macroaggregated albumin into the portal trunk in 28 patients with portal hypertension. 99mTc-macroaggregated albumin was injected through the catheter into the portal trunk and the intrahepatic shunt index was calculated by counting over predetermined areas of the liver and lungs. Injection of 99mTc-macroaggregated albumin into the spleen was performed between one or two weeks before portal vein catheterization, and the calculation of the intrahepatic shumlt index was performed with a scinticamera and ROI-radiograms obtained by data-processing. There was a significant correlation between the intrahepatic shunt index calculated by injection of 99mTcmacroaggregated albumin into the spleen and that by injection of 99mTc-macroaggregated albumin into the portal trunk (r=0.90, P<0.001).
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  • Effects of the combination of hyperthermia and adriamycin on cultured human liver cancer cells
    Kiyotaka FUJISE, Seishi NAGAMORI, Satoshi HASUMURA, Sadamu HOMMA, Haji ...
    1987 Volume 28 Issue 11 Pages 1467-1473
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1467
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    The responses of human liver cancer cells to the combination of hyperthermia and Adriamycin were investigated in vitro. An albumin high-producing cell line established in authors'labolatory (JHH-4) was used. Cells cultured on the Petri dishes at 37°C for 4 days, were changed with fresh mediums containing various concentrations of Adriamycin and transfered to the temperaturegradient-incubator and subsequently cultured for 2 days at 37-43°C. The viable cell counts and the amounts of secreted albumin were decreased along with the elevation of temperatures and Adriamycin concentrations. The uptake of labelled thymidine, uridine and leucine per dish was also decreased along with the elevation of two factors. Cultured cells on the Petri dishes were also affected by two factors morphologically. The effectiveness of the combination of hyperthermia and Adriamycin was stronger than that of the single use in every study.
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  • Experimental studies on extension of resectability of the liver in dogs
    with special reference to changes of lipid peroxide and effect of Coenzyme Q10
    Shuji YOSHIMINE
    1987 Volume 28 Issue 11 Pages 1474-1485
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1474
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    The purpose of this investigation was to elucidate pathophisiology and to obtain the survival of dogs after 84% hepatectomy, generally resulting poor prognosis. The study showed that the amount of lipid peroxide in the plasma and remnant liver increased much more after 84% hepatectomy than after 70% hepatectomy, causing significant impairment of the remnant liver function. To prevent hepatic dysfunction after hepatectomy, Coenzyme Q10, an antioxidant and membrane stabilizer, was administered, reducing the increase of lipid peroxide in the plasma and remnant liver, and restoring the liver function with subsequent good regeneration. The results of the present study suggest that the administration of Coenzyme Q10 may be useful for the recuperation of liver function and a more favorable prognosis following the extended hepatectomy.
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  • Alveolar hydatid disease of the liver made by inoculation of scolices into the liver of cottori rat
    Tomoyuki TAKAGI, Junichi UCHINO, Yukifumi KONDO, Naoki SATO, Yasunori ...
    1987 Volume 28 Issue 11 Pages 1486-1489
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1486
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    An experimental model for establishment of the treatment for alveolar hydatid disease of the liver was studied.
    Cotton rats, susceptible rodents (n=20) were used. Scolices were obtained from serially inoculated Abashiri strain in the cotton rat. 0.25ml of 7000 scolices/ml were inoculated in the liver. After eight and ten weeks localized alveolar hydatid diseases were recognized in 8 of the 9 livers. The cyst walls microscopically consisted of the germinal cell layer and cuticular layer and contained many scolices covered with the germ layer. This model seemed to be useful for establishment of treatment for hepatic alveolar hydatid disease of the liver.
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  • Two cases of severe hepatitis B by familial transmission
    Toshimi YOSHIDA, Seiji KAGA, Osamu MORIAI, Shu UEDA, Toshiaki CHIBA, K ...
    1987 Volume 28 Issue 11 Pages 1490-1496
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1490
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    Two survival cases of severe hepatitis by familial transmission are described.
    Acute severe hepatitis B had occurred in an infant born to a HBsAg carrier mother with positive anti HBe by vertical transmission. Subsequently fulminant hepatitis B occurred in the father by horizontal transmission, three months after the onset in the infant.
    A male child, 50 days old, was admitted to the hospital with high fever and decreased sucking ability. Serum GOT was 11, 380U, prothrombin time below 10% and HBsAg was positive. The patient's condition improved without developing into coma.
    The 27 year-old male (father), complained of nausea and general fatigue. Serum GPT was 4, 690U, prothrombin time 12% and HBsAg was positive. He developed hepatic coma, which subsequently improved with prednisolone, glucagon-insulin and branched chain-enriched amino acid solution therapy. These cases suggest that the treatment, for example HB immunoglobulin, is necessary for the prevention of hepatitis which occurred in the infant born to the HBsAg carrier mother with HBe antibody positive.
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  • A case of hepatocellular carcinoma with HBcAg in carcinoma cells
    Ai OKAJIMA, Hiroyuki KOKURYU, Seigo TAKAMATSU, Yukio OHSAKI, Taku HIGU ...
    1987 Volume 28 Issue 11 Pages 1497-1501
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1497
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    We studied the localization of tissue HBsAg and HBcAg in 102 cases of hepatocellular carcinoma (serum HBsAg was positive in 36 cases) by immunoperoxidase staining (PAP method). HBcAg was found in the nuclei of carcinoma cells in only one patient.
    This patient was a 37 year old man who was admitted to our hospital with right back pain. The liver was enlarged and hepatocellular carcinoma of diffuse type in the left lobe was suspected by ultrasonographic and angiographic examinations. The serum HBsAg and HBeAg were both positive but AFP was negative. He died five months later and the liver specimen was obtained with a Silverman needle.
    Histological examination showed hepatocellular carcinoma of Edmondson H-111 grade. HBsAg was found in the cytoplasm of carcinoma cells (diffuse type) and non-carcinoma cells (inclusion type). HBcAg was stained in the nuclei of carcinoma cells, suggesting the presence of HBV in carcinoma cells.
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  • A case of acute alcoholic hepatitis with appearance of abundant Mallory bodies in hepatitis B virus carrier state
    Yasushi MATSUURA, Eiji IDO, Masanao FUJIKAWA, Takashi MAEDA, Toshiji S ...
    1987 Volume 28 Issue 11 Pages 1502-1506
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1502
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    The case of a 49-year-old male HBV carrier who was a member of an HBV clustering family and died of hepatic failure during a month clinical course for acute alcoholic hapatitis is reported.
    Autopsy revealed diffuse sublobular hepatic necrosis including bridging necrosis, perivenular hyalinosis and pericelluar fibrosis. In addition, Mallory bodies were abundantly found in the hepatocytes, around which liver cell necrosis and neutrofil infiltration were remarkable. Orcein-dye positive hepatocytes were scattered, and HBc antigen in the nuclei was rarely found. Coexsistence of Mallory bodies and Orcein positive material was not observed in the same hepatocyte. These findings suggested that it was difficult for HBV to proliferate in the hepatocytes containing Mallory bodies impling the disturbance of the cytoskeleton system.
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  • A survived case of fulminant alcoholic hepatitis with multiple organ failure (MOF)
    Minoru FUKUI, Tomoyuki FURUKAWA, Mitsutoshi KUROSAWA, Hitoshi SATO, Hi ...
    1987 Volume 28 Issue 11 Pages 1507-1514
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1507
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    A survived case of a 60-year-old man who is habitual alcoholism rapidly fell into MOF with acute hepatic insufficiency due to excess alcoholic ingestion was reported. I thought this case acute hepatic failure on chronic liver disease (acute on chronic) by Sherlock. He had history of chronic alcoholic ingestion more than 80g of ethanol per day for twenty five years. He was admitted to our hospital because of fever, abdominal pain and icterus. On admission, hepatomegaly and vascular spiders on the skin were recognized. During clinical course, ascites and bleeding spots on the skin were recognized and his consciousness level was assessed in grade 3 hepatic encephalopathy.
    This case showed MOF which included acute hepatic insufficiency, disseminated intravascular coagulation, renal failure, gastrointestinal bleeding and heart failure. Plasma exchange, prednisolone and glucagon-insuline therapy were applied for acute hepatic insufficiency. The administration of gabexate mesilate was used for DIC. Peritoneoscopic and liver biopsy examination was findings of alcoholic hepatitis.
    This patient was a rare survived case of fulminant alcoholic hepatitis with MOF.
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  • A case of asymptomatic primary biliary cirrhosis with positive LE cell phemomenon
    Hiroomi MIYAZAKI, Hirohito TSUBOUCHI, Ichirou KIYAMA, Atsuyuki KAMIBEP ...
    1987 Volume 28 Issue 11 Pages 1515-1520
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1515
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    Primary biliary cirrhosis is frequently associated with another autoimmune diseases but rarely systemic lupus erythematosus (SLE) and lupoid hepatitis. We have experienced a rare case of asymptomatic PBC in a middle-aged woman, who showed a positive LE cell phenomenon. Laboratory data showed marked elevation of ALP, IgM, and positive anti-mitochondrial antibody (AMA) with titer of 1:2560 including positive anti-M2 and anti-M4 (subclass of AMA). A liver biopsy revealed PBC with stage 1/2 histologic characteristics. It is suggested that she was associated with SLE, because she was complicated with pleuritis and arthritis and showed a positive LE cell phenomenon and positive anti-nuclear antibody.
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  • A case of undifferentiated carcinoma of the liver
    Takashi OKAI, Yasuhiro KATO, Tetsuo MURAKAMI, Masashi UNOURA, Nobuyosh ...
    1987 Volume 28 Issue 11 Pages 1521-1526
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1521
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    A 55-year-old woman was referred to our clinic for evaluation of the cystic tumor of the liver. Although the imaging methods including hepatic angiography revealed a large hypovascular cystic tumor in the lateral segment of the liver, the preoperative diagnosis was not determined. The resected specimen revealed a blood-filled multicystic tumor with sharp boundaries. Histologically, the tumor was composed of an almost solid sheet of monotonous cells. In some areas there were numerous thin-walled blood vessels. A pseudopapillary appearance with vascular core was noted in some sections. There was almost no supporting collagenous stroma. The neoplastic cells were relatively small and generally rounded, but had ill-defined outlines. The cytoplasma was eosinophillic. Nuclei were irregularly round, moderately chromatic and had small inconspicuous nucleoli. No recurrence has been documented either clinically or on follow-up CT scans in the four years since surgery.
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  • Detection of collagen on plasma membrane of hepatocytes by aniline blue staining method
    Kooko SAKAKIBARA
    1987 Volume 28 Issue 11 Pages 1527-1528
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1527
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  • Anti-neutral glycolipid antibody in sera of patients with hepatocellular carcinoma after transcatheter arterial embolization (TAE)
    Masuo KASHIWAGI, Yusei IKEDA, Gotaro TODA, Toshiyuki MARUYAMA, Hiroshi ...
    1987 Volume 28 Issue 11 Pages 1529-1530
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1529
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1987 Volume 28 Issue 11 Pages 1531
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1531
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1987 Volume 28 Issue 11 Pages 1532
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1532
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1987 Volume 28 Issue 11 Pages 1533-1538
    Published: November 25, 1987
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.28.1533
    JOURNAL FREE ACCESS
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