Kanzo
Online ISSN : 1881-3593
Print ISSN : 0451-4203
ISSN-L : 0451-4203
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Volume 29, Issue 12
Displaying 1-24 of 24 articles from this issue
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  • Characterization of sinusoidal endothelial cells isolated from rat liver and the interrelation between cultured endothelial cells and hepatocytes
    Satoshi SHAKADO
    1988 Volume 29 Issue 12 Pages 1571-1578
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1571
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    Sinusoidal endothelial cells (SECs) and hepatocytes were isolated from a same rat liver by a collagenase digestion technique and centrifugal elutriation rotor. SECs formed branching network on non-collagen coated culture dishes and cobblestone appearance on type-1-collagen coated dishes. SEC presented characteristic fenestrae referred to as sieve plates in situ. The fenestration appeared as labyrinth-like structure, but not tube-like. Cultured SECs on collagen gel presented vascular-like space. In co-culture of hepatocytes and SECs, SEC extended their cytoplasmic processes around the hepatocytes and formed an interecellular space between two cells similar to the space of Disse. Collagen gel was useful in investigating the three-dimensional structure of the cultured cells.
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  • Uptake, intercellular transfer and intracellular translocation of retinoids in rat liver
    Hisataka MORIWAKI, Tetsuhiro KASAI, Tetsuya SHIMOMURA, Masataka OKUNO, ...
    1988 Volume 29 Issue 12 Pages 1579-1586
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1579
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    A study was conducted to investigate hepatic uptake of retinyl palmitate-labelled chylomicron and intercellular transfer as well as intracellular translocation of retinoids in rat liver.
    Following findings were obtained:
    1) Kinetic analysis of the plasma disappearance curve of chylomicron which was labelled in vivo by retinyl palmitate revealed that three compartment model has given the best fit to the curve and that plasma retinyl palmitate was cleared predominantly through the compartment 2, while approximately one eighth of whole plasma retinyl palmitate disappeared through compartment 3 which kinetic constant was found to be about one sixth of that of compartment 2.
    2) Twenty four hours after intraveneous injection of 14C-retinyl palmitate, 54% of the 14C taken up by the liver was found in stellate cells, and 86% of radioactivity in the stellate cell was already located in lipid droplets. Thus, it is suggested that, once received by the parenchymal cell, retinoid is rapidly transfered to the stellate cell and is deposited in the lipid droplets.
    3) Of retinyl hexadecylether and retinyl palmitate, only the latter was found to be possible to transfer from parenchymal to stellate cells. Since retinyl hexadecylether is unable to be hydrolysed by retinyl palmitate hydrolase in parenchymal cells, it is strongly suggested that, following the uptake of retinyl palmitate by parenchymal cells, hydrolysis of the compound to retinol is essential to transport retinoids from parenchymal to stellate cells for storage.
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  • Effect of ethanol on rabbit liver evaluated using in vivo P-31 magnetic resonance spectroscopy
    Takashi TAMADA, Fuminori MORIYASU, Shigeki ONO, Nobuyuki BAN, Yasunari ...
    1988 Volume 29 Issue 12 Pages 1587-1592
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1587
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    Changes in metabolic state of rabbit livers after administration of ethanol was evaluated using in vivo P-31 magnetic resonance (MR) spectroscopy. Targets were five normal control (NC) rabbits and four with chronically carbontetrachloride-damaged livers. A 1.5 Tesla whole-body MR imager was used for measurement. Two ml/kg of 50% ethanol solution was injected intravenously within about 1 minute. The inorganic phosphate peak decreased immediately after the injection. After ten minutes, it had dropped to 60% in the NC group and 85% in the damaged-liver group. The difference was statistically significant (p<0.05) and reflected differences in liver function of ethanol metabolism. The β-ATP peak decreased to 90% over 30 minutes in the NC group but there was little change in the damaged-liver group. There was almost no change in phosphomonoester in the NC group, but a slight increase was observed in the rabbits with damaged livers. No significant cytosolic pH variation was noted after ethanol injection in both NC and damaged liver.
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  • Effect of spironolactone on hepatic and systemic hemodynamics in liver cirrhosis without ascites
    Hidemasa OKUMURA, Hirokazu KOMEIJI, Takumi ARAMAKI, Katuaki SATOMURA, ...
    1988 Volume 29 Issue 12 Pages 1593-1598
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1593
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    The effect of spironolactone on hepatic and systemic hemodynamics were investigated in twelve patients with portal hypertension due to liver cirrhosis without ascites to verify whether it may reduce portal pressure. The measurements of hemodynamics were performed prior and after the oral administration of spironolactone at daily doses of 100mg for four weeks.
    The hepatic venous pressure gradient (as WHVP-FHVP) significantly decreased by 24.7%. (baseline 16.9±3.6mmHg, spironolactone 12.6±4.2mmHg, p<0.01). However, no effect was on estimated hepatic blood flow and portal venous blood flow. Spironolactone caused a significant reduction of mean arterial blood pressure, cardiac output, plasma volume, body weight, and central venous pressure, while no change was observed in heart rate, systemic vascular resistence and hematocrit. The reduction rate of hepatic venous pressure gradient following spironolactone administration was not significantly correlated with the change of above mentioned parameters.
    These preliminary results suggest that spironolactone has a hypotensive effect on portal pressure in patients with cirrhosis. Factors other than vasoactive action should be considered as the possible mechanism for spironolactone induced hypotensive effect on portal pressure.
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  • Evaluation by computed tomography of effects of transcatheter therapy for liver cancer
    Mitsuo TSUBAKIMOTO, Kenji NAKAMURA, Ryoichi MATSUO, Katsuko MURATA, Ke ...
    1988 Volume 29 Issue 12 Pages 1599-1604
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1599
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    We compared the antitumor effects of transcatheter arterial embolization and infusion chemotherapy by evaluation of CT images before and after treatment. We looked for decreased tumor size and the appearance of low-density areas without contrast enhancement. In 37 of the 38 patients (97%) undergoing embolization, low-density areas appeared, but tumor size did not decrease. In all ten patients who received infusion chemotherapy, tumor size decreased, but low-density areas did not appear. These findings suggest that the anti-tumor effect caused by ischemia differs from that caused by anticancer drugs, and that on CT images, the former effect can be observed as the appearance of low-density areas, but the latter effect can be observed as a decrease in tumor size.
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  • Role of laparoscopic vascular changes in fatty liver
    Comparative study with intrahepatic microvasculature using experimental model
    Hitomi KARUBE, Kohdoh ISHII, Keiji SHIRASAKI, Masanao SUGIMOTO, Shigek ...
    1988 Volume 29 Issue 12 Pages 1605-1611
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1605
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    Laparoscopic vascular pattern in the course of fatty liver was investigated comparing with intrahepatic microvasculature of fatty liver induced by choline deficient diet (CDD) in the rat. Early laparoscopic change seen was an increase in number of portal branches. Microangiograms of early experimental lesion represented amorphously increased portal branches in the peripheral area, indicating principal vascular changes in fatty liver are deranged portal system. Dilatation, irregularity and tortuosity in the portal system became conspicuous with progression of the disease. Laparoscopic evidence for arterioles were reflected not only by intrahepatic inflammation, but by parallel development of regenerative nodules. Arteriolar vascularization seen in the stroma was characteristic in early cirrhotic stage, whereas it was pronounced on the surface in enlarged nodules. Experimental model showed that the larger the nodules, the more hypovascular in the nodules. It is concluded that capsular vasculature in fatty liver is closely related to the intrahepatic vascular disorder, and microcirculatory derangement is highly involved in the progression of the disease.
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  • Study on 1, 25-dihydroxyvitamin D3 receptor in human liver cancer tissue
    Yuuji SAKAI, Yoshihiro FUKUDA, Itsuo YAMAMOTO, Shigeharu DOKOH, Hiroo ...
    1988 Volume 29 Issue 12 Pages 1612-1618
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1612
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    In order to investigate the expression and the significance of 1, 25-dihydroxyvitamin D3 receptor (VD-R) in liver cancer, VD binding activity to the cytosol fraction was studied by radio-receptor assay in surgical specimens obtained from the patients with 18 hepatocellular carcinoma (HCC), 3 cholecystcholangiocarcinoma (CC) and 3 metastatic liver cancer (MLC; 1 case from breast cancer, 2 cases from colon cancer). The binding activity in surrounding non-cancerous liver tissues (3 normal liver, 1 chronic hepatitis, 4 liver cirrhosis which were histologically proven) was also examined.
    VD-R was not detected in all normal liver tissues nor in non-cancerous tissues with histological findings of chronic hepatitis and liver cirrhosis. On the contrary, VD-R was detected in 10 out of 18 tissues with HCC. In positive cases, Scatchard analysis revealed the Kd values ranging from 0.1 to 2.0×10-9M and the receptor concentration from 1.5 to 26 fmol/mg cytosol protein. All showed histological features of grade II or III by the criteria of Edmondson. In 8 negative cases, one showed histological finding of well differentiated type, and 6 of the remaining cases were treated with transcatheter arterial infusion of anti-cancer drugs within one month before operation. VD-R were also detected in 2 of 3 with CC and all of 3 with MLC.
    To our knowledge, this is the first report showing the expression of VD-R in HCC different fron non-cancerous liver tissues and suggesting the possible significance of VD-R in hepatocarcinogenesis or differentiation of cancer cells.
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  • Survey and follow-up study of primary liver cancer in Japan
    Report 8
    Liver Cancer Study Group of Japan
    1988 Volume 29 Issue 12 Pages 1619-1626
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1619
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    The Liver Cancer study group of Japan analysed statistically 7320 cases of primary liver cancer diagnosed from Jan. 1, 1984 to Dec. 31, 1985 in 507 hospitals throughout the country. In 2514 cases, a histological diagnosis was available. They comprised 2300 cases of hepatocellular carcinoma (HCC), 138 of cholangiocellular carcinoma 27 of mixed carcinoma, 11 of hepatoblastoma, 3 of sarcoma, and 35 others.
    The survey, based mostly on the histologically proven 2514 cases, included gross and histological features, modality of metastasis, pathology in noncancerous portions of the liver, past medical history, frequency of positive HBsAg, and anti-HBs, age distribution, diagnostics, and treatments including surgical procedures, transcatheter arterial embolization and chemotherapy.
    In addition, cumulative survival rates of the HCC patients with partial hepatectomy from Jan. 1, 1978 to Dec., 31, 1985 were calculated by the life table method according to the curability, and also, survival rates according to the degree of tumor development were computed to clarify the prognostic indicators of HCC patients.
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  • Hepatitis B virus (HBV) carriers who developed acute hepatic failure
    Hidetoshi YODA, Kendoo KIYOSAWA, Takeshi SODEYAMA, Eiji TANAKA, Yukio ...
    1988 Volume 29 Issue 12 Pages 1627-1632
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1627
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    Seven HBV carriers who developed severe acute exacerbation and acute hepatic failure were reported. None of them had evidence of superinfection by delta agent, hepatitis A virus, Epstein-Barr virus and cytomegalovirus, and there was no patient with alcoholic liver disease and drug induced liver injury.
    Of 4 patients without history of blood transfusion, 3 cases were positive for HBV specific DNApolymerase (HBV-DNA-p) and HBV-DNA at the onset of acute exacervation, suggesting that active replication of HBV contributed to the developement of acute hepatic failure.
    To the contrary, all 3 patients with history of blood transfusion were negative for HBV-DNA-p, HBV-DNA and IgM HBc antigen at the onset of acute exacervation, which occurred in 1 to 2 months after blood transfusion, thus the superinfection of non-A, non-B hepatitis virus could be responsible for the occurrence of acute hepatic failure in these cases.
    Glucagon-insulin administration and plasmaexchange therapy were done for all patients, but failed to recover except for one cases, suggesting that the prognosis of acute hepatic failure evolving from chronic liver diseases was extremely poor.
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  • Drug-induced allergic hepatitis caused by glycyrrhizin, or extract of licorice root
    Koichi AKASHI, Masafumi SHIRAHAMA, Ryuichi IWAKIRI, Hironobu YOSHIMATS ...
    1988 Volume 29 Issue 12 Pages 1633-1637
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1633
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    Stronger-Neo-Minophagen C (SNMC) consists of glycine, cysteine and glycyrrhizin, a main component of Kanzo or licorice. It is an established drug for allergic diseases and chronic liver diseases in Japan. We treated a patient who developed SNMC-induced allergic hepatitis, an extremely rare complication with SNMC.
    The patient was a 33-year-old Japanese man who was admitted to Kyushu University Hospital as a diagnosis of viral meningitis. On admission, laboratory tests revealed slight elevation of SGPT and negative test for HBs antigen and HBc and HA IgM antibody. Although the meningitis was improved, the SGPT level elevated gradually, and intravenous administration of SNMC (20ml/day) was initiated. However, acute elevation of SGOT and SGPT were observed eight days after the initiation of SNMC therapy, when the administration of SNMC was stropped. Two months lator, SGOT and SGPT levels were normalized, and the challenge test with 5ml of SNMC was performed with positive reaction. From these results, the diagnosis of drug-induced allergic hepatitis caused by SNMC was established.
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  • Therapeutic effect of estradiol benzoate on primary biliary cirrhosis -A case report-
    Shigeki MATSUKI, Kohdoh ISHII, Keiji SHIRASAKI, Masanao SUGIMOTO, Shig ...
    1988 Volume 29 Issue 12 Pages 1638-1642
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1638
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    A case of asynptomatic primary biliary cirrhosis (a-PBC) succesfully treated with estradiol benzoate (EB) was reported. This 35-year-old female was admitted to the Kitasato University Hospital complaining of general fatigue. The liver function tests showed a mild elevation of bilirubin, accompained by an elevation of transaminase, alkaline phosphatase (ALP) and γ-glutamyltranspeptitase (γ-GTP) levels. Serum immnoglobulines of all three major classes were elevated, but elevation of IgM was most conspicuous with the degree of 395mg/100ml. Also demonstrated was positive test for anti-mitochondrial antibody. Biopsy of the liver disclosed PBC (stage 2-3 of Scheuer) with piecemeal necrosis and portal=portal bridging. The patient received 1mg EB every one or two weeks. Estradiol treatment was followed by return toward normal values of ALP and γ-GTP. Histology obtained at 16 and 33 months of treatment showed disappearance of piecemeal necrosis and portal=portal bridging, respectively. No remarkable side effect was observed in the course of treatment. The observation suggests that EB has a salutary effect on PBC.
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  • A case of hepatocellular carcinoma associated with renal hypouricemia
    Yukiji OTANI, Masamichi YAMADA, Miho SAKURAI, Takeshi AZUMA, Toshiyuki ...
    1988 Volume 29 Issue 12 Pages 1643-1648
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1643
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    A 34 year-old woman (HB carrier) developed diffuse type hepatocellular carcinoma (HCC) (Edmondson Grade III) associated with renal hypouricemia. Laboratory findings revealed elevation of serum AFP (259000ng/ml) and marked hypouricemia (1.3mg/dl). A 24-hour excretion of uric acid was not decreased; renal clearance studies showed high ratio of urate to creatinine clearance, whereas creatinine clearance was normal, indicating that the hypouricemia was due to excessive renal excretion of uric acid. The tumor rapidly progressed to be resistant to a course of therapy and she died of DIC following pneumonia. Serum uric acid remained very low throughout. Several factors that would affect the uric acid level were excluded, and only minimal abnormalities of other tubular functions were detected. The underlying mechanism (s) that led to abnormal renal handling of uric acid was unknown. We would suggest that renal hypouricemia which was thought to be related to tumor metabolism (tumoral hypouricemia), should be considered one of clinical signs of paraneoplastic syndrome in patients with HCC.
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  • An autopsy case of mixed hepatocellular and cholangiocellular carcinoma
    Tetsuya MURATA, Shozo WATANABE, Satoru MIURA, Hideki TAKANARI, Masayas ...
    1988 Volume 29 Issue 12 Pages 1649-1653
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1649
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    An autopsy case of 61-y.o. woman with mixed hepatocellular and cholangiocellular carcinoma was reported with immunohistochemical investigations. The histological examination revealed uniform proliferation in both primary and metastatic sites, which consist of cells with both two characteristics of hepatocellular and cholangiocellular nature, and thus concluded mixed hepatocellular and cholangiocellular carcinoma. The elevation of serum AFP, CEA, and CA19-9 confirmed the mixed nature of this tumor. CEA and TPA were positive in cytoplasm of tumor cells immunohistochemically. These findings suggested that this tumor arose from bipotential cell, which represented hepatocellular and cholangiocellular characteristics.
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  • Cytotoxic T-cell response to acetaldehyde-pulsed cells in alcoholic liver disease
    Namiki IZUMI, Yoshinori SAKAI, Yasushi HASUMURA
    1988 Volume 29 Issue 12 Pages 1654-1655
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1654
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese]
    1988 Volume 29 Issue 12 Pages 1656
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1656
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1988 Volume 29 Issue 12 Pages 1657
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1657
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1988 Volume 29 Issue 12 Pages 1658
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1658
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1988 Volume 29 Issue 12 Pages 1659
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1659
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1988 Volume 29 Issue 12 Pages 1660
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1660
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1988 Volume 29 Issue 12 Pages 1661
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1661
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese]
    1988 Volume 29 Issue 12 Pages 1662
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1662
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1988 Volume 29 Issue 12 Pages 1663
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1663
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1988 Volume 29 Issue 12 Pages 1664
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1664
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1988 Volume 29 Issue 12 Pages 1665-1696
    Published: December 25, 1988
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.29.1665
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