Kanzo

Effect of betamethasone on albumin synthesis in isolated perfused liver of normal and CCl₄ intoxicated rats

Yoichiro KATO

1975 Volume 16 Issue 1 Pages 1-9
Published: January 25, 1975
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.16.1

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The effect of betamethasone on albumin synthesis was studied in isolated perfused liver of normal and CCl₄ intoxicated rats by ¹⁴C-carbonate method.
Albumin synthesis rate in normal livers averaged 5.4mg/100g body weight, and the rate increased to 8.7mg after addition of betamethasone.
The synthesis rate in CCl₄ intoxicated livers in acute, chronic and cirrhotic stage averaged 4.3mg, 3.3mg and 2.8mg respectively. The rate after addition of betamethasone increased to 7.3mg, 5.5mg and 4.5mg respectively.
From these results obtained, albumin synthesis rate decreased in accordance with the progress of the liver injury, and betamethasone increased albumin synthesis rate in both normal and CCl₄ intoxicated livers.
The synthesis rate after addition of actinomycin D averaged 5.0mg, but the rate averaged 5.2mg after addition of both actinomycin D and betamcthasone in normal livers.
From these results, it seemed that actinomycin D have no effect on albumin synthesis in normal livers, but inhibit to increase the albumin synthesis rate after addition of betamethasone.

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Clinical significance of serum guanase activities determined by ammonia method using 8-azaguanine as substrate

Susumu ITO, Takeshi TAKAOKA, Seiichiro KISHI, Hiromichi OKUDA, Setsuro ...

1975 Volume 16 Issue 1 Pages 10-17
Published: January 25, 1975
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.16.10

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Serum guanase activities were determined in normal subjects and in various disease, and the guanase activities of the various tissues and the cell fractions of the liver of human were also determined the new method, which measure the ammonia liberated from 8-azaguanine by the action of guanase with direct colorimetric method. Liver, kidney and brain had high contents of guanase. Heart, skeletal muscles and pancreas almost did not show guanase activity, while they showed comparatively higher activities of GOT and GPT. Such differences of the distribution of guanase and GOT were not only observed in the various tissues but also were observed in the cell fraction of the liver. The activities of the serum guanase elevated markedly in liver diseases, but they did not elevate in myocardial infarction, polymyositis and dermatomyositis, in which the activities of the serum GOT and GPT elevated slightly.
These data indicated that the serum guanase activities reflected the hepatic injury more specifically than the GOT and GPT activities. It was concidered from these results that the determinations of the serum guanase activities by the ammonia method might be useful for the clinical practice.

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Utilization of Glutathione for BSP Conjugation in the rat liver

by means of the double-labelling technique

Michitaka SHIMONO, Hiroshi SUZUKI, Toshitsugu ODA

1975 Volume 16 Issue 1 Pages 18-24
Published: January 25, 1975
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.16.18

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To study the hepatic function of conjugation and excretion, the radioactivities (R.I.) of the BSP-GSH (BSP conjugated with glutathione) in the bile after intravenous administration of radioactive GSH (glutathione), cysteine or glycine to the rat were investigated. Especially by means of the double-labelling technique, we investigated the utilization of glutathione for BSP conjugation in the rat liver. When ³⁵S-GSH and ¹⁴C-1C-glycine GSH were simultaneously administered i.v., ³⁵S and ¹⁴C were incorporated into the BSP-GSH with the equal ratio to that of the injected GSH.
From these it is most likely that GSH is taken in the original form into the hepatic cells and utilized for conjugation.
³H-GSH (2C-glycine³H) and ³H-glycine (2C) induced lesser excretion of 3H incorporated into BSP-GSH in the bile than that when ³⁵S-GSH or 14C-glycine (2C) was administered, i.e. the ratio of ³⁵S/³H was 116.
Less ³H excretion shows that ³H compounds are easily metabolized after going through the hepatic cell wall.

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Hepatic Dye Transport in Constitutional Hyperbilirubinemia and Constitutional ICG Excretory Defect

Toshihiko NAMIHISA, Masaji NAMBU, Norio KOBAYASHI, Yuji YAMASHIRO

1975 Volume 16 Issue 1 Pages 25-35
Published: January 25, 1975
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.16.25

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Changes of hepatic dye transport were studied in twelve cases of Gilbert's disease (Gilbert), twelve cases of Dubin-Johnson syndrome (Dubin-Johnson), eleven cases of Rotor's type of hyperbilirubinemia (Rotor) and sixteen cases of constitutional ICG excretory defect.
In Gilbert the BSP disappearance curve showed two different patterns. One group revealed reduced initial disappearance and another group showed decreased disappearance in the second exponential component. The ICG disappearance curve was divided into two groups. One group showed normal disappearance and another group showed moderately or apparently decreased disappearance in the second exponential component.
In all cases of Dubin-Johnson the BSP disappearance curve showed a secondary rise during from 45 to 60 minutes after an injection, and ICG disappearance curve revealed the phenomenon in four of seven cases during from 50 to 90 minutes after an injection.
Although the ICG disappearance curve in constitutional ICG excretory defect showed similar pattern to that of Rotor, the step formation on ICG disappearance curve was observed during from 20 to 25 minutes after an injection in all cases.
It is suggested that the depressed hepatic dye clearance is due to decrease both in uptake and excretion of dye by the liver in Rotor and is due to supPresed excretory function in Dubin-Johnson.
From these results it is concluded that changes of hepatic transport are different in Gilbert, Dubin-Johnson, Rotor and constitutional ICG excretory defect.

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Studies on the infection of the HB antigen between wives and husbands

Osamu KURIMURA, Kinzaburo HAMADA, Haruko KAGAWA

1975 Volume 16 Issue 1 Pages 36-39
Published: January 25, 1975
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.16.36

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1) According to the statistical analysis of the answer of 72 persons, whose spouses were HB antigen carriers, ten patients (13.9% of total) had suffered from the liver disease after their marriage. Interesting enough, one of these 10 patients died of liver cirrhosis 7 years after marriage.
2) HB antigen could not be detected in none of twenty-six cases, whose spouses were HB antigen carriers, whereas 12 cases (46.2%) revealed HB antibody. This incidence (46.2%) of HB antibody positivity was significantly higher than that (23.1%) among the general population in same city.
3) 6 out of 10 hepatitis cases with positive HB antigen had supouses who were supposed to be HB antigen carrier with high antigen titer.
And the most interesting thing in this report is that HB antigen associated hepatitis developed within one year of the marriage with the spouses who were HB antigen carriers.

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Studies on the infection of the HB antigen between mothers and children

Osamu KURIMURA, Kinzaburo HAMADA, Haruko KAGAWA, Yoshimi ARAMITSU, Hid ...

1975 Volume 16 Issue 1 Pages 40-44
Published: January 25, 1975
Released on J-STAGE: January 19, 2010

DOIhttps://doi.org/10.2957/kanzo.16.40

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1) Out of 8 infants, whose mothers were HB-Ag carrier at their delivery, 2 infants became HB-Ag carrier after 6 months from their birth.
2) Anti HB-Ag antibody was detected in 55 cases of 238 mothers at their delivery, and in 42 cases of 238 new born babies. The antibody in the serum of the new born babies was disappeared in all cases after 6 months.
3) The authors reported a case of two years male child, whose mother was the HBAg carrier, died of subacute hepatitis.

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Studies on the subpopulation and function of peripheral lymphocytes in patients with liver diseases, and the inhibitor to PHA stimulation existing in patient's serum.

Masahiro NAKAO, Yasuhiro MIZOGUCHI, Takeyuki MONNA, Sukeo YAMAMOTO, Se ...

1975 Volume 16 Issue 1 Pages 45-56
Published: January 25, 1975
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.16.45

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In our recent report we observed the abnormal response to PHA stimulation in patient's peripheral lymphocytes with chronic liver diseases.
Present report concerns the extensive studies of this earlier report; analysis of subpopulation and function of peripheral lymphoyctes in patients with liver diseases.
The PHA responsiveness in acute hepatitis was reduced at acute phase and improved at convalescent stage. In chronic liver diseases, a half of patients showed a decrease of Tcell population as well as reduced response to PHA stimulation. These phenomena were observed more frequently in HB-antigen positive patients than in HB-antigen negative cases.
Furthermore, we found the existence of the inhibitor to PHA stimulation in the serum of 8 cases among 19 patients with various liver diseases.
In patients with such serum inhibitor, the reactivity of lymphocytes themselves to PHA was considerably higher but T-cell population was much more decreased than those of inhibitor negative cases.

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1975 Volume 16 Issue 1 Pages 57
Published: January 25, 1975
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.16.57

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