Kanzo
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ISSN-L : 0451-4203
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Volume 26, Issue 7
Displaying 1-22 of 22 articles from this issue
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  • Clinical evaluation of HBeAg/anti-HBe system in HBeAg positive chronic hepatitis
    Mikio MATSUO
    1985 Volume 26 Issue 7 Pages 819-829
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.819
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    The purposes of this study is to observe the changes of HBeAg/anti-HBe system and to predict the prognosis of patients with HBeAg positive chronic hepatitis. This study consists of ninety patients with HBeAg positive chronic hepatitis who were followed for two to ten years (mean 4.3 years). Diagnosis of liver disease was made according to the findings of liver biopsy and HBeAg and anti-HBe were determined by RIA method.
    Clearance of HBeAg was observed in 36 of 90 cases and appearance of anti-HBe in 18 of these 36 during observation period. Cumulative clearance rate of HBeAg at five years after observation was 45.2 percent. Furthermore, cumulative seroconversion rate from HBeAg to anti-HBe was 25.4 percent at five years. The clearance rate and seroconversion rate were higher in females, cases under 35 years old and cases with elevated serum level of GPT over 300 KU. Clearance of HBeAg was not shown in cases with lower level of GPT under 100 KU. HBeAg in chronic active hepatitis with bridging necrosis was cleared in early phase of this study. Many in cases with persistent positive HBeAg progressed to liver cirrhosis or hepatocellular carcinoma. However, some cases with bridging necrosis or lobular disorganization and/or with higher serum level of GPT over 100KU at least four times during this study, developed to liver cirrhosis or hepatocellullar carcinoma.
    These result led to conclude that clearance of HBeAg or seroconversion to anti-HBe did not always reflect good prognosis.
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  • Studies of short-term prednisolone therapy in children with HBeAg positive chronic type B hepatitis
    Masanori YASUDA, Hiroshi TANAKA, Nobuyuki ADACHI, Masaru SHIMIZU, Yosh ...
    1985 Volume 26 Issue 7 Pages 830-837
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.830
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    We have studied the effect of short-term prednisolone therapy in 9 children with HBeAg positive chronic type B hepatitis, aged 6 to 13 years. All children were started on treatment with prednisolone (1.0-1.5mg/kg/day) orally at the level of s-GPT being more than 140IU/L (140-673IU/L). Prednisolone was decreased and discontinued gradually for period of 21 to 36 days.
    During follow-up study for 13 to 19 months after treatment, HBeAg disappeared from serum in 8 out of 9 children (88.9%). 5 out of 9 children (55.6%) have seroconverted from HBeAg to anti-HBe. Only one cleared HBsAg by RIA. 8 children in whom HBeAg disappeared achieved a complete and protracted remission.
    All children were free of severe side effects with this therapy.
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  • Comparative histopathological investigation of hepatocellular carcinoma with and without hepatitis B virus DNA integration
    Hiromu AOYAMA, Okio HINO, Tomoyuki KITAGAWA, Wataru MORI
    1985 Volume 26 Issue 7 Pages 838-845
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.838
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    As to 20 hepatocellular carcinoma (HCC) autopsy cases, the histopathological features of HCC were compared between with and without hepatitis B virus (HBV) DNA integration. All 6 HCCs with HBV DNA integration were with liver cirrhosis and histologically classified as trabecular type HCC (4 were Edmondson's grade II, 2 were grade III). In 14 HCCs without HBV DNA integration, 9 were classified as trabecular type, 3 were pseudoglandular type and 2 were compact type (10 cases were Edmondson's grade II, 4 were grade III). In addition to the histological typing, the examination of other histological markers revealed that frequent appearance of intracytoplasmic globular hyaline bodies (4/6) in HCCs with HBV DNA integration, but low frequency (1/14) in HCCs without HBV DNA integration. In conclusion, although there were a little difference between the histology of two groups HCC, comparative histopathological investigation of HCCs with and without HBV DNA integration did not reveal any characters specific for the HCC with viral integration.
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  • State of hepatitis B virus DNA in liver of patients with hepatitis B surface antigen positive liver diseases: Its variation and its correlation with HBV serological markers
    Shuichi KANEKO
    1985 Volume 26 Issue 7 Pages 846-855
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.846
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    The state of HBV-DNA in liver by Southern hybridization, its variation, and its correlation with HBV serological markers were studied in 37 patients with HBs antigen positive liver disease. The state of HBV-DNA in liver could be classified into two types: Type A had singlestranded (ss), partially double-stranded (pds) HBV-DNA which was detected predominantly in the liver of HBe antigen positive chronic active hepatitis, Type B had no ss and pds but doublestranded HBV-DNA which was detected predominantly in the liver of after anti-viral treatment. HBV-DNA in liver was detected in 10/12 HBe antigen negative patients and surpassed DNA polymerase and HBV-DNA in serum for the detection of HBV-DNA. Our observations suggest the examination of HBV-DNA in liver is useful for the evaluation of the pathogenesis and the prognosis of chronic type B hepatitis.
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  • Liver orcein stain and DHBV DNA
    Junko MORI, Masao OMATA, Osamu YOKOSUKA, Fumio IMAZEKI, Yoshimi ITO, K ...
    1985 Volume 26 Issue 7 Pages 856-860
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.856
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    Liver orcein stain and duck hepatitis B virus (DHBV) DNA in sera and livers were examined in 106 ducks (44 Chinese ducks, 15 Japanese ducks and 47 Japanese duckings). Orcein positive hepatocytes were found in 18 of 38 (47%) DHBV DNA seropositive ducks.
    Serial analyses of DHBV DAN by Southern blot and spot hybidizations in experimentally infected Japanese ducklings revealed a dissociation between the amount of viral DNA in sera and the emergence of orcein positive hepatocytes. Orcein positive hepatocytes were generally associated with presence of viral infection for at least 4 to 6 months.
    Accumulation of orcein positive material in liver cells may be one of the common properties of hepadna viruses, and this stain may be utilized for screening new hepatitis B virus-like viruses.
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  • Clinical evaluation of serum tissue polypeptide antigen (TPA) in patients with hepatocellular carcinoma
    Kimihiro SHIRAISHI, Masatoshi TANAKA, Yasuhiko KUBO, Fuyuhiko NINOMIYA ...
    1985 Volume 26 Issue 7 Pages 861-866
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.861
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    S-TPA was measured in 54 patients with hepatocellular carcinoma (HCC) and 75 patients with liver cirrhosis (LC).
    A mean value of 216.9±157 (sd) u/l in HCC was significantly higher than that of 155.4±91u/l in LC. However only in 23 of 54 patients with HCC, s-TPA was elevated above normal range (180u/l). While the changes of s-TPA levels before and after transcatheter arterial embolization or intra-arterial chemotherapy reflected the effects of these therapies. The values of sTPA was significantly correlated to those of serum transaminase and alkaline phosphatase in HCC and LC.
    From these findings, it is suggested that the elevation of s-TPA level is due to tumor production, damage of hepatocytes and cholestasis, and that s-TPA is, however, not a practical tumor marker in screening of HCC, but it is useful for monitor to the therapeutic effects particularly in AFP low producing cases.
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  • Detection of HAV in the epithelial cells of interlobular bile ducts of marmosets with acute hepatitis A
    Mitsuhiko MORIYAMA
    1985 Volume 26 Issue 7 Pages 867-876
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.867
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    Standard thin-section electron microscopy and light microscopic immunoperoxidase antibodymethod were carried out on livers of marmosets with experimental acute hepatitis A for the presence of HAV in the epithelial cells of interlobular bile ducts.
    Virus-like particles were found in cytoplasmic vesicles of interlobular bile duct epithelial cells by standard thin-section electron microscopy, They measured 26-28nm in diameter and were morphologically identical to HAV particles previously described in hepatocyte cytoplasms of infected marmosets.
    In addition, by immunoperoxidase antibody-method, HAV antigen was detected in epithelial cells of interlobular bile duct. It is possible that the multiplication of HAV may occure not only in hepatocytes and Kupffer cells, but also in epithelial cells of interlobular bile duct.
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  • Induction of macrophage-mediated hepatocytotoxicity by culture supernatant from LPS-activated Kupffer cells
    Yasuhiro MIZOGUCHI, Takayuki ARAI, Hiroko KATOH, Hiroko TSUTSUI, Keiji ...
    1985 Volume 26 Issue 7 Pages 877-882
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.877
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    Since the macrophage activation judging from the increased uptake of 3H-glucosamine into cells was observed by treatment of macrophage with culture supernatant from LPS-activated Kupffer cells, LPS-stimulated Kupffer cells were appeared to produce some substance caused the activated of macrophages and activated macrophages exhibited their hepatotoxic actions through the secretion of cytotoxic substance or direct contacts to target cells.
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  • Total bile acid in chronic liver disease
    Kenichi FUKUOKA
    1985 Volume 26 Issue 7 Pages 883-890
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.883
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    To estimate the usefulness of total bile acid (TBA) as the liver function test, the serum level of fasting TBA (F·TBA) and maximum TBA (M·TBA) in patients with chronic liver disease were measured by enzymatic fluorometry.
    The levels of F·TBA in 67% of the patients and that of M·TBA in 98% of the patients were higher than those in the normal control group.
    These results indicate that the measurement of M·TBA in useful for screening and evaluating chronic liver disease.
    Both F·TBA and M·TBA were significantly correlated with ICG and BSP retention and hepaplastin test. Therefore, it was suggested that the measurement of TBA might provide a more sensitive test for estimating the hepatic reserve than other liver function tests currently being used.
    It was found that the patients with severe esophageal varices had a significantly the higher level of TBA and the heart to liver ratio of T1-201 which method was developed to estimate the portal systemic shunt.
    These results suggested that the elevated level of TBA reflects an increasing of portal systemic shunt and TBA might be a good indication of portal systemic shunt.
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  • Metabolism of 3β, 7β-dihydroxy-5β-cholan-24-oic acid in man
    Change of the bile acid levels in the peripheral and portal blood, and the excretion into the bile, after the oral administration
    Masaji NAMBU, Yoko OIKAWA, Toshihiko NAMIHISA
    1985 Volume 26 Issue 7 Pages 891-897
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.891
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    300mg of 3β, 7β-dihydroxy-5β-cholan-24-oic acid (3β, 7β-diOH) was oraly administered in three patients with choledocholithiasis in whom external biliary fistula was established.
    The change of serum level of 3β, 7β-diOH was different in three patients, and the peak levels in the peripheral blood were 27.5μg/ml, 3.7μg/ml and 94.5μg/ml, respectively. Appearance of ursodeoxycholic acid (UDCA) in the peripheral blood and the change of serum level after 3β, 7β-diOH administration was different in all patients.
    There was no relation between 3β, 7β-idOH levels in the peripheral and portal blood obtained at the same time from patients after the administration.
    Bile was collected by an hour through external biliary fistula during 24 hrs after the oral administration of 300mg 3β, 7β-diOH. 3β, 7β-diOH was not detected in the bile. However, UDCA clearly increased in the bile from three or four hrs after the 3β, 7β-diOH administration and it persisted for about 10 hrs. Other bile acid levels excreted into bile did not show significant changes.
    These results indicate that 3β, 7β-diOH is well absorbed from the intestine and are tranported via the portal blood to the liver. In the liver it undergoes transformation to 3α-hydroxy epimer and is excreted as UDCA into bile.
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  • A fluorimetric and enzymatic determination for the estimation of total bile acid sulfates in urine
    Hitoshi AMAKASU, Takatoshi MIYAKAWA, Hidetoshi HIRAKAWA, Yasutoshi KON ...
    1985 Volume 26 Issue 7 Pages 898-904
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.898
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    A fluorimetric and enzymatic determination for the estimation of total bile acid sulfates in urine is described. The sulfate fraction was obtained from a urine specimen by passing through a Sep-pak C18 cartridge, followed by group separation by ion-exchange chromatography on a lipophilic gel, piperidinohydroxypropyl Sephdex LH-20.
    The obtained sulfate fraction was solvolized by the modified method of Galeazzi et al and deconjugated bile acids were estimated with fluorimetrc and enzymatic method.
    Overall recovery of glycine- and taurine-conjugated bile acid 3-sulfates from normal urine ranged from 90.5 to 93.7% and uncojugated bile acid 3-sulfates from 48.7 to 78.0%. The range of normal values for total bile acid sulfates in urine was found to be 2.6-17.5μmoles/l (mean: 10.4±4.9) for males and 1.6-15.3μmoles/l (mean: 6.5±4.9) for females.
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  • Utility of dynamic computed tomography in diffuse liver diseases
    Koichi FUJIKAWA, Akira INAGAWA, Tatsushi YOKOYAMA, Toshiyuki IWAMOTO, ...
    1985 Volume 26 Issue 7 Pages 905-912
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.905
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    We tested the diagnostic abilities of dynamic CT in diffuse liver diseases. The material includes 23 cases of chronic active hepatitis (CAH), 32 cases of liver cirrhosis (LC) and 15 cases with normal liver.
    For each case, time-density curve was obtained from the right lobe of the liver. To allow numerical evaluation of the curve, gamma variate fit techniques were employed. Changes in the curves were analyzed by comparing three parameters-rise time (RT), decay time (DT) and corrected first moment (MC)-derived from two coefficients of the fitting equation.
    Values of three parameters increased with the severity of the diseases reflecting prolonged curves with delayed peak and gradual downslope in damaged livers.
    MC values showed most significant correlation with the degree of the diseases. High MC value (>95) were associated with 30 cases of LC and 3 cases of CHA, and moderate MC value (70<MC≤95) with 19 cases of CAH and 2 controls, and low MC value (≤70) with 15 controls and a case of CAH. We conclude that dynamic CT time-density study with gamma variate fltting is useful in the differential diagnosis of the diffuse liver diseases.
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  • A survival case of infantile fulminant hepatitis B born to HBsAg carrier mother with HBe antibody positive in vertical transmission of hepatitis B virus
    Sousuke TERAZAWA, Akira TAKAO, Hiroo MATSUI, Seizi YAMAGUCHI, Manabu Y ...
    1985 Volume 26 Issue 7 Pages 913-920
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.913
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    Prevention of vertical transmission of hepatitis B virus (HBV) by hepatitis B immune globulin (HBIG) and HB vaccine has been carried out in our laboratory since 1979.
    It was reported that acute hepatitis B caused by vertical transmission of HBV had occurred in infants born to HBsAg carrier mother with HBe antibody positive, and it was also inferred that infantile fulminant hepatitis B would occur in infants born to HBsAg carrier mother with HBe antibody positive.
    We experienced such a case who could survive without handicaps, the patient had infantile fulminant hepatitis B at two month old. Blood exchanges four times, branched amino-acld rich solutions, steroid therapy were done effectively. HBV antigen-antibody system of his mother was HBs-Ag 25 positive (RPHA method), HBeAb positive (RIA method), and that of the infant was HBsAg negative, HBsAg 26 positive (PHA method), HBeAg negative, HBeAb positive and HBcAb positive (respectively RIA method).
    IgM class HBcAb of the infant in cut off index was 8.4 in acute phase, 0.6 in convalescent phase, so we diagnosed infantile fulminant hepatitis B through the vertical transmission of HBV by HBsAg carrier mother with HBe antibody positive.
    Now, we proposed the necessity of the treatment for the prevention of vertical transmission of HBV by HBsAg carrier mother with HBe antibody positive.
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  • A case of chronic active hepatitis type B with severe liver dysfunction induced by steroid withdrawal therapy
    Improved by remedication of corticosteroid
    Masaru SHIMIZU, Nobuyuki ADACHI, Hajime TOKITA, Kazuo NAKURA, Akira SE ...
    1985 Volume 26 Issue 7 Pages 921-927
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.921
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    It was reported that steroid withdrawal therapy was beneficial in the management of patients with HBsAg- positive chronic active hepatitis and rarely led to fulminant hepatitis. We report a case of chronic hepatitis B who developed severe liver dysfunction by steroid withdrawal therapy, but rapidly improved by readministration of corticosteroid.
    A 34-year-old man with HBsAg- and HBeAg- positive chronic hepatitis received steroid withdrawal therapy at the time of elevated transaminase (S-GOT 91I.U./l, S-GPT 260I.U./l). The dosage of prednisolone was 40mg/day at the first week, then decreased by 10mg everyweek. Total doses were 700mg in 4 weeks. Two rebound S-GPT peaks were observed at one month and three months after the discontinuation of prednisolone. At the second S-GPT peak, he complained of increasing general fatigue and loss of appetite. The laboratory data showed severe liver dysfunction (S-GOT 2147I.U./l, S-GPT 2379I.U./l, T.B.11.6mgldl, Prothrombin time 33%. Thrombotest 15.7%, Normotest 18.2% and the serum methionine 132.5nMOL/ml). Ascites was observed by the abdominal CT scanning. Then he received prednisolone therapy, 40mg/day at the beginning, tapering gradually, His liver function tests and general condition improved rapidly.This case demonstrates the effectiveness of re-administration of prednisolone in the case of severe liver dysfunction induced by withdrawal of steroid.
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  • A case of combined hepatocellular and cholangiocellular carcinoma associated with von Meyenburg complex
    Yutaka INAGAKI, Hisashi KIDANI, Yoshinobu HINOUE, Masashi UNOURA, Nobu ...
    1985 Volume 26 Issue 7 Pages 928-933
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.928
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    A 69-year-old man, who had been diagnosed as HBsAg-positive chronic acitve hepatitis eight years earlier, was admitted to our hospital because of jaundice. He was emaciated, and the physical examination revealed icteric conjunctivae and hepatomegaly. The level of serum bilirubin was 2.14mg/dl, and that of AFP was 5720ng/ml. Ultrasonography and CT scan disclosed a large SOL in the porta hepatis and the dilatation of the intrahepatic bile ducts. He was clinically diagnosed as hepatocellular carcinoma associated with cirrhosis, and he died of hepatic failure forty-eigth days after admission. The necropsy showed a large tumor in the porta hepatis with portal vein thrombosis.
    The histopathological findings were not only trabecular pattern but also tubular structure which contained the Alcian blue-positive material in the lumen. From these findings this tumor was diagnosed as combined hepatocellular and cholangiocellular carcinoma. Furthermore, von Meyenburg complexes were seen around the portal tracts and even among the tumor cell nest, and this finding suggested the possibility that the tumor might arise from von Meyenburg complex.
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  • A case of hepatocellular carcinoma accompanied by DIC after transcatheter arterial embolization
    Yukio OHSAKI, Taku HIGUCHI, Seigo TAKAMATSU, Tatuo SHIMIZU, Megumu UED ...
    1985 Volume 26 Issue 7 Pages 934-939
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.934
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    Transcatheter arterial embolization (TAE) is, now, the established treatment for hepatocellular carcinoma. The purpose of this paper is to report an exceptional case with a complication of DIC after TAE. A 53-year-old male was hospitalized because of high serum AFP. Ultrasonography, CT scan and angiography suggested hepatocellular carcinoma with intrahepatic metastasis. TAE was performed four times repeatedly. After the fourth TAE, the diagnosis of DIC was made based on coagulation test, high FDP and low F. VIII activity. With appropriate treatment, he became free from DIC. But the tumor, which had invaded the diaphragma, ruptured into the thoracic cavity and he died. There have been many reports on TAE, but few are discussed on its complications. In this paper a severe complication "DIC" triggered by TAE is reported. After TAE procedure, it is necessary to follow the patient with repeated coagulation tests cautiously.
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 7 Pages 940
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.940
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 7 Pages 941
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.941
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 7 Pages 942
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.942
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 7 Pages 943
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.943
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    1985 Volume 26 Issue 7 Pages 944
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.944
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1985 Volume 26 Issue 7 Pages 945-986
    Published: July 25, 1985
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.945
    JOURNAL FREE ACCESS
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