Kanzo

Histochemical visualization of 2', 5'-oligoadenylate in liver cells of patients with hepatitis before and after interferon treatment

Seijin NADANO

1992 Volume 33 Issue 11 Pages 817-824
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.817

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This study was performed to examine the localization of 2', 5'-oligoadenylate (2-5A) and the antiviral status in the liver using indirect immunoenzymatic technique and double staining. 2-5A in the liver was observed in the cytoplasm of hepatocytes of 42 out of 46 patients with viral hepatitis. In chronic hepatitis type B, hepatocyte 2-5A was stained more strongly 2-5A during the increasing phase of DNA-polymerase activity than during the peak or decreasing phase (p<0.05).
Under the immune electron microscopy, 2-5A was found in the cytosol of cytoplasmic matrix, but not in the nucleus.
In double staining, the simultaneous presence of HBeAg was significantly lower in hepatocytes strongly staining for 2-5A (p<0.001). This indicates that 2-5A inhibits viral replication.
By interferon (IFN) treatment, the increase of 2-5A in hepatocytes was found in 7 out of 11 patients with chronic active hepatitis type B. In 3 of 7 patients with increased 2-5A, their sera were found to have converted to HBeAg negative.
These results indicate that 2-5A plays a role in antiviral activity in situ.

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Anti-interferon antibody in chronic hepatitis patients receiving interferon treatment

Ken ARAI

1992 Volume 33 Issue 11 Pages 825-832
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.825

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I studied anti-interferon (IFN) antibody in serial serum samples obtained from 58 patients with chronic hepatitis B and C treated with IFN (HLBI, recombinant IFN-α 2a or-α 2b, HuIFN-β). Anti-INF antibody was measured by Western blotting. Anti-INF antibody was detected in seven (3.8%) of the 183 samples. Two patients treated with recombinant IFN-α 2a developed neutralizing anti-IFN antibOdies with the development of clinical resistance to IFN therapy, while none of the 56 patients treated with either HLBI, recombinant FN-α 2b or HulFN-β developed anti-IFN antibody. Anti-IFN antibody was developed in one patients one month after IFN administration. I conclude that Western blotting used in this study is a useful method to evaluate the incidence and clinical significance of anti-IFN antibody.

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Serological subtype of HBsAg and genetical classification of partial S gene

Ken-ichi OHBA, Masashi MIZOKAMI, Yasuo INA, Kaoru SUZUKI, Tomoyoshi OH ...

1992 Volume 33 Issue 11 Pages 833-838
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.833

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HBsAg has been devided into 4 subtypes by serological methods. We try to make the classification of the S gene (nt. 295-528), which code HBsAg, by the molecular evolutional techniques. We constructed the phylogenetic tree for the S gene (nt. 295-528), and classified into the genetical subgroups on the tree. Then, we compared with subtypes and genetical subgroups. The tree shows that 51 strains in this study can be classified into the 4 genetical subgroups that are not always consistent with the serological subtypes. Furthermore 18 adw strains can be classified into the 3 genetical subgroups. So, we concluded that same subtypes are not always nearer than other subtypes at the molecular levels.

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Immunohistochemistry of transforming growth factor β1 in acute liver injury

Sadataka INUZUKA, Takato UENO, Takuji TORIMURA, Houhi KO, Ryuichiro SA ...

1992 Volume 33 Issue 11 Pages 839-848
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.839

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We investigated relationships between immunolocalizations of transforming growth factor β1 (TGF-β) and inflammatory cells, the immunolocalization of fibronectin (FN) and type III collagen (type III col.) of the rat liver, and plasma FN level during early stage of acute liver injury induced by a heated needle. 6 hours after injury (6H): TGF-β and FN were intensely visible in the necrotic areas with numerous inflammatory cells. TGF-β was observed in platelet granules and also in the rough endoplasmic reticulum (rER) of inflammatory cells. 24H: FN was observed in the rER of hepatocytes. 48H: TGF-β and type III col. were observed in the rER of Ito cells, endothelial cells or hepatocytes. Plasma FN level, decreased rapidly 1H, and increased 24H. These results suggest that in injured areas of the liver, TGF-β could be produced by platelets and inflammatory cells at the very early stage of injury, and may be involved in FN distribution accompanied with inflammatory cells, and suggest that TGF-β could be also produced by Ito cells, endothelial cells and hepatocytes, and may be involved in EN and type III col. production by these cells.

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Nuclear DNA analysis of resected hepatocellular carcinoma and recurrence

Toshiya KAMIYAMA, Junichi UCHINO, Yoshie UNE, Hiroyuki ISHIZU, Yasuaki ...

1992 Volume 33 Issue 11 Pages 849-854
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.849

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Nuclear DNA analysis on 81 cases of hepatocellular carcinoma (HCC) were performed comparing recurrent rates, recurrent modes, and survival rates. DNA ploidy pattern was divided in diploid and aneuploid. Thirty-eight cases (46.9%) were diploid pattern and 43 were aneuploid respectively. Recurrence rate of diploid group was 60.5% and 65.1% in aneuploid group. The most frequent site of recurrence was intrahepatic in both groups. The time interval by the appearance of recurrent disease was 24.2 months in diploid group and 14.4 months in aneuploid group. In diploid group, the intrahepatic recurrence was seen with single nodule, while multiple nodule in aneuploid group. Significantly higher frequency of recurrence was found in aneuploid group. Re-resection of recurrent hepatic tumor resulted the most favorable prognosis in both groups. In the patients treated by TAE or intraarterial chemotherapy, diploid group showed better results compared with aneuploid group. Nuclear DNA analysis of HCC with recurrence promises the useful method to evaluate the biological potential of malignancy of HCC.

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Evaluation of new monoclonal antibody; Ber-EP4, for the differentiation hepatocellular carcinomas from metastatic liver cancers

Tomohiro SHIRO, Toshihito SEKI, Taiichi NAKAGAWA, Yoshihiro TAGAWA, Ya ...

1992 Volume 33 Issue 11 Pages 855-862
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.855

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The new mouse monoclonal antibody: Ber-EP4 reacts with two glycoproteins of 34 and 49 kD present on the surface and in the cytoplasm of all epithelial cells, except the superficial layers of squamous epithelia, hepatocytes, and gastric parietal cells. In present study, 164 formalin-fixed, paraffin-embedded various cancers were examined for immunoreactivity with Ber-EP4 by means of a sensitive immunoperoxidase technique.
In the liver, Ber-EP4 intensely labeled with the bile duct, but not normal hepatocytes. Characteristically, Ber-EP4 reacted with all tested carcinomas originated from colorectum, stomach, biliary duct, pancreas, thyroid gland, ovary kidney, breast, and lung, but did not react with hepatocellular carcinoma (HCC). Further AFP-producing adenocarcinoma with hepatoid feature was positive for this antibody. So, Ber-EP4 might prove useful for the differentiation HCC from other matastatic liver tumors, especially HCC resembling carcinomas, and also for the diagnosis of bile duct related diseases.

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Analysis of local production of inflammatory cytokines in primary biliary cirrhosis by reverse transcription-polymerase chain reaction

Tsubasa MORITA, Tetsufumi ITO, Kiichiro AYATA, Kazuhisa SATO, Mikio NI ...

1992 Volume 33 Issue 11 Pages 863-871
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.863

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Local production of inflammatory cytokines, such as interleukin 1β (IL-1β), interleukin 6 (IL-6) and tumor necrosis factorα (TNF-α) in the liver tissues obtained from patients with primary biliary cirrhosis (PBC) has been analyzed by reverse transcription-polymerase chain reaction. In addition, serum levels of those cytokines and their production by peripheral blood mononuclear cells (PBMCs) stimulated with PHA were also examined. In the sera, there were no significant differences of inflammatory cytokines between PBC patients and healthy controls. Production of those cytokines from PBMCs stimulated with PHA, was significantly increased in PBC patients. Intensity of the cytokine genes in the liver of PBC patients were expressed stronger than that of healthy controls by a densitometric analysis of amplified products on the gels. Expression of IL-6 gene was correlated with the serum IgM levels, and was, reversely correlated with serum albumin levels. Increased expressions of IL-1β and TNF-α were found at the stages of Scheuer III and IV as compared with I and II. They were also increased in symptomatic PBC compared with asymptomatic PBC patients. These findings suggest that upregulation of inflammatory cytokines is associated with the pathogenesis of PBC.

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A case of chronic active hepatitis type C associated with autoimmune reaction who responded to interferon therapy

Kyosuke KAJI, Koichi NISHIMURA, Toru SAKAMOTO, Masayu TAKEUCHI, Shuich ...

1992 Volume 33 Issue 11 Pages 872-876
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.872

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A 53-year-old woman was admitted because of elevation of transaminase levels (alanine aminotransferases 306 IU/l, asparate aminotransferase 392 IU/l). While anti-HCV antibody (C100-3) and HCV RNA tested positive, serological data including high gammaglobulinemia (3.5g/dl) and presence of antinuclear antibody suggested mechanism of autoimmune reaction for the hepatitis as well as histological findings of chronic active hepatitis. Although she was initially treated with prednisolone, levels of transaminase did not change. Following interferon-α therapy, levels of transaminase simultaneously normalized within 4 weeks of treatment. Furthermore, liver biopsy after treatment demonstrated improvement of lymphocyte infiltration around portal area and piecemeal necrosis. This case represents that some of patients with chronic active hepatitis C respond well to interferon therapy whereas they suggest the presence of autoimmune mechanism.

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A case of HTLV-1 carrier complicated with amebic liver abscess ruptured into Stomach and biliary tree

Tatsunobu KARASAWA, Shinichi KATO, Hiroshi NOZAWA, Yoshio AIZAWA, Miki ...

1992 Volume 33 Issue 11 Pages 877-881
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.877

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We report a case of HTLV-1 carrier who had amebic liver abscess with rare complication. The patient was a 48-year-old male HTLV-1 carrier. He visited our hospital because of epigastric pain and fever. On examination, his liver was extremely enlarged and tender. Abdominal CT scan and echogram revealed a mass in the left hepatic lobe (S4). The diagnosis of amebic liver abscess was confirmed because the serological test for amebiasis showed positive. Percutaneous transhepatic drainage was performed, and contrast medium filled the abscess cavity. It revealed the abscess cavity communicated with stomach and biliary tree. Though this complication has been thought to be severe and sometimes fatal, his symptoms resolved completely and the lesion became absent by the drainage and Metronidazole treatment.

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Successful IFN therapy for reactivation of hepatitis B virus infection induced by steroid therapy in a patient with polymyositis

Takeaki SATO, Syouji NAGASE, Takashi SUGIMURA, Takayoshi FUKUTOMI, Mas ...

1992 Volume 33 Issue 11 Pages 882-885
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.882

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A 28-year-old man was addmitted to our hospital in November 1990 with a complaint of muscle weakness. He was diagonosed as polymyositis, diffuse panbronchiolitis and hepatitis B virus carrier. After the patient was treated with corticosteroids, polymyositis improved but serum AST, ALT and DNA-P elevated. The liver biopsy showed chronic active hepatitis. So we decided to treat the patient with interferon. After the treatment by interferon, the liver function markedly improved without exaceration of the polymyositis. To our knowledge, this is a first report of interferon therapy for the patient with hepatitis B virus complicated by polymyositis.

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Hepatic hydrothorax without ascites of about two years duration in a patient with liver cirrhosis

Shogo IGARASHI, NAOYA Funaki, Yasushi TAKEUCHI, Yukifusa TANAKA, Shige ...

1992 Volume 33 Issue 11 Pages 886-890
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.886

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Sixty two year old male patient with liver cirrhosis was admitted with sudden onset of dyspnea. Massive right pleural effusion was detected and thoracocenthesis had been Performed repeatedly. Two months later, the effusion was equilibrated in the right lower pleural cavity. Ascites was not demonstrated by CT-SCAN and US. No malignant cells and bacteria were detected in the effusion. 1 mc of 111In solubilized in physiological saline was injected intraperitoneally. Three hours later, almost all the radioactivity of 111In was demonstrated in the right pleural cavity by scintigraphy. Rapid and massive accumulation of the right pleural effusion began two years later, again, and patient died with respiratory failure. Autopsy showed a defect of 5mm of diameter in the right diaphragm, and traction of ascites into pleural cavity by negative pressure via the diaphragmatic hole is supposed to be the mechanism of this hepatic hydrothorax.

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Intravariceal tumor thrombi formation in hepatocellular carcinoma-A case report-

Koichiro MIKI, Kazuhiro TAKAHASHI, Minoru NAKAMURA, Yasuhiko HIRATA, J ...

1992 Volume 33 Issue 11 Pages 891-895
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.891

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The formation of tumor thrombi in esophageal varices in hepatocellular carcinoma has rarely been reported. We herein report a case with such a rare complications.
A 66-year-old man was admitted because of abdominal fullness and general fatigue. Ultrasonography and computed tomography disclosed a massive tumor in the right hepatic lobe and tumor thrombus in the main portal trunk. Fiberscopy disclosed esophageal varices. Angiographic examination revealed neovascularity and arterio-portal shunt in the right hepatic lobe, and subsequent visualization of esophageal varices. The patient died of intraperitoneal bleeding from the tumor. When autopsied, a massive tumor with multiple satellite lesions was evident. Compact type, moderately differentiated hepatocellular carcinoma was revealed by the microscopic examination. Tumor thrombi were found in the portal trunk and esophageal varices. Microscopically, lung metastasis was disclosed and the metastatic route from the liver via the portal vein through the esophageal varices to the lung was considered.

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Clinical and histopathological study of hepatocellular carcinoma with marked elevation of serum interleukin-6 level

Toshihito SEKI, Tomohiro SHIRO, Taiichi NAKAGAWA, Masayuki WAKABAYASHI ...

1992 Volume 33 Issue 11 Pages 896-897
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.896

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Detection of HCV associated antigen in human chronic hepatitis C liver

Mitsuhiko MORIYAMA, Fumihiko KOMINE, Keizu FUJIBAYASHI, Junko MORIYAMA ...

1992 Volume 33 Issue 11 Pages 898-899
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.898

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1992 Volume 33 Issue 11 Pages 900
Published: November 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.900

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