Kanzo

A Relationship between Differentiation and Phenotypic Expression in Cultured Epithelial like Cell from Rat Liver.

Kiwamu OKITA, Takahiro KODAMA, Kenichi NODA, Tadayoshi TAKEMOTO

1976 Volume 17 Issue 12 Pages 863-868
Published: December 25, 1976
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.17.863

JOURNAL FREE ACCESS

Show abstractHide abstract

We have reported that a-fetoprotein and PN-antigen are expressed in premalignant hepatocyte populations, in vivo. Hloweve, it is still unclear whether these antigens are related cell-differentiation. Therefbre, we studied on differences of phenotypic expression in in vrtro system, using cultured epithelial like cells from the rat liver and cultured hepatoma cells. As shown in Table, it was found out that cultured epithelial likc cells have dlifferent expressions in each. These data also suggest that one of the difficulties of establishment of normal liver cell culture is due to different phenotypic expression in each cell line. As the result, afetoprotein and PN-antigen may be antigens which associate to differentiation.
We also tried to detect A-and C-type viruses in the cultured cells by means of electron microscopic technique and found out them in high incidence in the cells which seemed to be transformed. However, the role of such oncorna virus in the cells was unclear.

View full abstract

Download PDF (5765K)
The Effect of Liver Hydrolysate on Rat Liver Regeneration

Susumu MITANI, Katsuhiko SUGAHARA, Nobuhiro KAWANO, Tetsuya SHIRAKURA, ...

1976 Volume 17 Issue 12 Pages 869-873
Published: December 25, 1976
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.17.869

JOURNAL FREE ACCESS

Show abstractHide abstract

This preliminary study was perfbrmed in order to stimulate regeneration of cirrhotic liver caused by partial hepatectomy by giving liver hydrolysate containing precursors of nucleic acids and oligopeptides.
In normal rat liver, a proper medication of the liver hydrolysates gave rise to significant increases both in incorporation of ³H-TdR into the DNA of regenerating liver and in the mitochondrial ATP synthesis of liver.
The cells of experimental cirrhotic liver were observed to have a low adenine nucleotide pool and a reduced energy charge similar to those of normal regenerating liver following hepatectom. The cirrhotic liver cells, moreover, showed some delay in increase in mitochondrial ATP synthesis, essential to liver regeneration.
It was suggested that proper medication of liver hydrolysates to a cirrhotic liver would increase nucleotide pool and also mitochondrial ATP synthesis resulting support and stimula-tion of liver regeneration.

View full abstract

Download PDF (336K)
Study on pathogenesis of galactosamine induced hepatitis in rats

Kenichi NODA

1976 Volume 17 Issue 12 Pages 874-884
Published: December 25, 1976
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.17.874

JOURNAL FREE ACCESS

Show abstractHide abstract

Phenobarbital (Ph) or 3-methylcholathrene (3MC) pretreatment inhibited D-galactosamine (gal) induced hapatic injury, i.e. increment of serum GPT, hepatic tissue damage, PAS positive granular appearance in hepatocyte and decreasement of protein synthesis.
Cytochrome P-450 (P-450), which is a drug metabolizing enzyme in microsomes, increased relatively by gal (200 mg/kg body weight) injection and slightly decreased by gal (400 mg/kg body weight) injection, did not increase by uridine or glycyrrhizin treatment after gal treatment.
The fraction of P-450 was represented as two main bands by means of SDS-polyacrylamide gel electrophoresis of hepatic microsomal protein. The pattern of electrophoresis changed by Ph or 3MC treatment, but unchanged by gal injection.
It was suggested that pathogemesis of gal induced hepatic injury was not always due to the defficiency of uridine, while it was prevented by pretreatment of drugs to induced RNA synthesis such as phenobarbital, 3-methylcholanthrene.

View full abstract

Download PDF (7490K)
Studies on hepatic cell uptake of ICG

Fumihiko OHYA, Takahiko OHYA

1976 Volume 17 Issue 12 Pages 885-893
Published: December 25, 1976
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.17.885

JOURNAL FREE ACCESS

Show abstractHide abstract

Indocyanine green (ICG) aqous solution showed the very low degree of dissociation at room temperature and the characteristic bands of infra-red spectra due to the coordinated water molecules were observed in the region of 650 to 420 cm^-1.Low activation energies of reactions of ICG with serum proteins or hepatic cytoplasmic proteins were calculated from the plot of Log I, vs l/T. Kinetics of removal of ICG from the blood indicated that the removal of this dye from the blood in rat obeyed Michaelis-Menten kinetics.
It was suggested from these results that the absorption at 710mμ and 780 mμ was due to coordinated ICG with water molecules, the chemical bonding type between ICG and serum proteins or hepatic cytoplasmic proteins could be considered as a sort of coordinated bond and ICG molecules passed through the hepatic plasma membrane by means of a carriermediated transport mechanism.

View full abstract

Download PDF (1754K)
The clinical study of intravenous galactose tolerance test in liver diseases

Shigeyuki TAKAMORI, Toshio SAKANAKA, Hiroyuki HASEGAWA, NAKAZIMA, Dai ...

1976 Volume 17 Issue 12 Pages 894-906
Published: December 25, 1976
Released on J-STAGE: January 19, 2010

DOIhttps://doi.org/10.2957/kanzo.17.894

JOURNAL FREE ACCESS

Show abstractHide abstract

This study investigates the significances of intravenous galactose tolerance test in the patients with various liver diseases. Galactose was given by rapid intravenous injection (350mg/kg. standard body weight) to 139 subjects, and blood galactose was determined by a specific galactose oxidase method.
The mean values of T1/2 (the half life of injected galactose) were 11.5±1.5minutes (SD) in normal adults, 43.1 minutes in subacute hepatitis, 21.9 minutes in acute stage of acute hepatitis, 16.3 minutes in recovery stage of acute hepatitis, 16.5 minutes in inactive type of chronic hepatitis, 18.7 minutes in active type of chronic hepatitis, 24.9 minutes in liver cirrhosis, 16.4 minutes in gallstone (obstructive jaundice), 13.9 minutes in diabetes mellitus (without liver damage), and 10 minutes in Dubin-Johnson Syndrome. Statistically significant correlations were found between T 1/2 values and histological findings of liver specimens such as liver cell necrosis, liver cell degeneration and liver fibrosis. And also the significant correlations were noted between T 1/2 values and standard liver function tests such as BSP test, ICG test, γ-globulin, total protein, ZTT, GOT, GPT and choline esterase levels. It was suggested that the prolongation of T 1/2 values was as clinically significant as retardation of ICG test, in diagnosing the patients with liver cirrhosis.

View full abstract

Download PDF (918K)
Epidemiological Study on Subtype of HBs-Ag and Anti-HBs

Atsushi MAEDA, Katashi MATSUNO, Mutsuo UECHI, Daizo YAMAUCHI, Katsuko ...

1976 Volume 17 Issue 12 Pages 907-913
Published: December 25, 1976
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.17.907

JOURNAL FREE ACCESS

Show abstractHide abstract

The subtypes of HBs-Ag and anti-HBs Were examined on 1391 subiects of the periodic multiphasic health tests performed by the Institute for Adult Diseases, Tokyo Women's Medical College and 90 members of the families of 20 probunds who had liver diseases and were admitted by the Institute of Gastroenterological Medicine (8 of acut hepatitis, 6 of chronic hepatitis, 3 of liver cirrhosis, and 3 of hepatocellular carcinoma).
The antigen positive rate of the suhjects of the tests was 1.4%, and their antibody positive rate was 27.4%, while those of the lineage of liver diseases Were 41.1% and 34.4%, respectively; the latter were obviously higher than the former, and particularly the antigen positive rate of the latter was higher.
As to the subtypes, adr of the antigen and R of the antibody Were predominant both in tbe subjects of the tests and the lineage of liver diseases. In the linage of liver diseases, the group of liver cirrkosis and hepatocellular carcinoma showed accumulation of antigen and antibody in brothers, sisters and children. Only one family having the adw subtype was of acute hepatitis, and the rest families had all adr and R. The subtype was never different in a family.

View full abstract

Download PDF (464K)
Studies on familial clustering of cases With discrepancy between indocyanine green (ICG) test and bromosulfophthalein (BSP) test

Takanao AOKI, Takehiko INAGAKI, Hiroshi SASAKI, Humihiro ICHIDA

1976 Volume 17 Issue 12 Pages 914-921
Published: December 25, 1976
Released on J-STAGE: January 19, 2010

DOIhttps://doi.org/10.2957/kanzo.17.914

JOURNAL FREE ACCESS

Show abstractHide abstract

One patient with a defect in indocyanine green (ICG) metabolism and With hepatic cirrhosis, including slight bromosulfophthalein (BSP) retension, was presented.
Dye-kinetics studies showed that both values of transport maximum (Tm) and relative storage capacity (S) were very loW, and transfer rate of ICG was supposedly disturbed in a step of leaviug the plasma to enter into the hepatic cells.
Other 5 cases of his family showed similar discrepancy between ICG-test and BSP-test.
Gel filtration studies showed no difference between the patient and normal-control.

View full abstract

Download PDF (458K)
[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1976 Volume 17 Issue 12 Pages 922
Published: December 25, 1976
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.17.922

JOURNAL FREE ACCESS

Download PDF (70K)
[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1976 Volume 17 Issue 12 Pages 923
Published: December 25, 1976
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.17.923

JOURNAL FREE ACCESS

Download PDF (90K)
[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1976 Volume 17 Issue 12 Pages 924
Published: December 25, 1976
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.17.924

JOURNAL FREE ACCESS

Download PDF (81K)
[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

1976 Volume 17 Issue 12 Pages 925
Published: December 25, 1976
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.17.925

JOURNAL FREE ACCESS

Download PDF (96K)
[title in Japanese]

1976 Volume 17 Issue 12 Pages 926-941
Published: December 25, 1976
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.17.926

JOURNAL FREE ACCESS

Download PDF (4500K)

Register with J-STAGE for free!