Kanzo

Detection of HCV associated markers in the serum of spouses of patients with type C chronic liver disease

Kiminori KIMURA

1993 Volume 34 Issue 3 Pages 187-193
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.187

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To evaluate the inter-spouse transmission as one of the infectious routes of HCV. 48 patientspouse pairs were studied. Out of 48 patients with type C chronic liver disease, 14 (29%) had spouses with seropositive HCV markers.
Eight spouses had C100-3 antibody and core antibody. They had also HCV RNA and showed abnormal liver function tests. Other 6 spouses had core antibody without C100-3 antibody. Two of these 6 spouses had HCV RNA and showed high value of ZTT test. HCV genotype was matched within 7 patient-spouse pairs and different in other 3 pairs. These results suggest that inter-sponse transmission may exist certainly as one of the infectious routes of HCV, but other environmental factors also may not be ignored.

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The carrier rate of Hepatitis B virus among Vietnamese refugees in Japan

Susumu NAKATA

1993 Volume 34 Issue 3 Pages 194-197
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.194

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The auther studied the carrier rate of hepatitis B virus among Vietnamese refugees in Japan. 2428 subjects were investigated (male: female=1587: 841, Age range was between 9 month and 86 years of age). 316 of 2428 (13%) subjects were HBsAg positive. The positive rate of HBsAg in male and female is 243/1587 (15.3%) and 73/841 (8.8%), respectively. The positive rate of HBsAg in the group between 10 and 19 years of age (19.9%) is much higher than that in the group between 0 and 9 years (11%). It is speculated that intravenous drug using, prostitution, unsanitary medical care and bloodtransfusion without adequate screening test may cause transmission of HBV in addition to that between mother and child.
In conclusion, this result may suggest that the prevalent rate of hepatitis B in Vietnam is about 13% in general population.

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Change of anti-HCV antibody titer during interferon therapy

Investigation of NS3, NS4 and core region antibody

Yoshiyasu KARINO, Syuhei HIGE, Akiyoshi SAGA, Takashi MATSUSHIMA, Tamo ...

1993 Volume 34 Issue 3 Pages 198-204
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.198

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Antibodies to hepatitis C virus (HCV) were measured quantitatively by enzyme immunoassay using NS3, NS4 and core region synthetic peptide in patients with type C chronic hepatitis during and after interferon (IFN) therapy. In excellent and good response cases, NS3 and NS4 region antibodies decreased significantly at the end of IFN therapy and all 3 antibodies decreased significantly after 6 months of IFN therapy. In contrast, NS4 and core region antibodies decreased significantly in unchanged response cases at the end of IFN therapy, but returned pre-treatment value after 6 months of IFN therapy. The HCV-RNA measured by RT-PCR technique became negative in most cases irrespective of clinical response. To predict the further prognosis at the end of IFN therapy, determination of NS3 region antibody quantitatively would be a convenient probe.

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Proliferative response of peripheral blood mononuclear cells to recombinant hepatitis C virus (HCV) antigens in patients with HCV infection

Tetsufumi ITO, Kazutaka KUROKOUCHI, Keiji ARIMA, Tsubasa MORITA, Mikio ...

1993 Volume 34 Issue 3 Pages 205-211
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.205

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The proliferation of peripheral blood mononuclear cells (PBMC) in responses to recombinant hepatitis C virus (HCV) core region antigen (rcoreAg), nonstructural 3 region antigen (rNS3Ag), and nonstructural 5 region antigen (rNS5Ag), were studied in patients with chronic HCV infection, and in control group. Significant proliferative responses of PBMC to all antigens were detected in patients when compared with the control group (p<0.01).
After separated of CD4⁺ cells and CD8⁺ cells from PBMC taken from patients with chronic active hepatitis (CAH-C), the proliferative responses to recombinant antigens were determined. The CD4^- CD8⁺ PBMC responded to recombinant antigens, but not the CD8^- CD4⁺ PBMC. The population of CD8⁺ CD11b^- cells (cytotoxic T cell) was increased, when PBMC was stimulated for 96 hours by rNS3Ag.
A correlation has been noticed in proliferation of PBMC to rNS3Ag and transamynase level in CAH-C (r=0.64).
These results suggest that recombinant HCVAg specific lymphocytes (cytotoxic T cell) may contribute to liver damage in HCV infection.

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A simple estimation of liver collagen content by the computed color image analyzer using Srius red stained sections

Hitomi NAKAYASHI, Seigo TAKAMATSU, Hiroshi NAKANO

1993 Volume 34 Issue 3 Pages 212-218
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.212

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We studied the quantitative determination of liver collagen content by the computed color image analyzer using fibrous tissue stained sections. 1) To determine the dye that specifically binds to collagen, we examined the binding capacity of Sirius red, Aniline blue, and Acid fuchsin for extracellular matrix components in liver. Then it was revealed that Sirius red had the most specific binding capacity for collagen. 2) For twenty four liver samples from autopsies or hepatic operations the following stains were performed, double stain of Sirius red and Fast green (SF stain), Azan stain and van Gieson stain. And the percentage of fibrotic area to the total area (% fibrosis) was measured by the computed analyzing system. Only by using SF stain, % fibrosis could be measured without any difficulties. 3) There was a highly significant correlation between the biochemically measured hydroxyproline content of liver samples and the % fibrosis of same samples with SF stain (r=0.887).
This simple method may be useful for the quantitative determination of liver collagen content from biopsied samples.

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Anti-microtubule autoantibodies in liver disease

Mitsuo GOTO

1993 Volume 34 Issue 3 Pages 219-227
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.219

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Autoantibodies to microtubule constituent proteins, 210 kDa microtubule associated protein and tubulin were estimated in various liver diseases by EIA. Serum from 88 patients (18 alcoholic liver disease (ALD), 50 chronic viral liver disease (CVLD), 20 PBC) and 18 normal controls were used for study. 1) The frequency of anti-210 kDa MAP autoantibody in ALD and PBC was 61.1% and 30%, respectively. 2) In stage IV of PBC, the frequency of anti-210 kDa MAP autoantibody was significantly higher than that in stage I. 3) The frequency of anti-tubulin autoantibody in PBC was 60% in controls. 4) In group IV of PBC, the absorbance of anti-tubulin IgA and IgM was significantly higher than those in group I, respectively. Autoantibodies to microtubule constituent protein were frequently detected in ALD and PBC and useful to diagnose as histological study in PBC.

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A patient with chronic active hepatitis B who lost serum HBsAg spontaneously accompanied by histological healing

Kazunori KUMADA, Masashi MIZOKAMI, Hideyuki KANO, Kenji SAKAKIBARA, No ...

1993 Volume 34 Issue 3 Pages 228-233
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.228

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A male patient with type B chronic active hepatitis verified by the liver biopsy (1st biopsy) in May 1979 was presented. Serum aminotransferase levels became normal in Aug. 1981. Serum HBsAgbecame undetectable by r-PHA method in Sep. 1982 and by RIA method in Apr. 1985.
Both 2nd and 3rd liver biopsies in Jun. 1983 and in Aug. 1987 respectively showed non-specific change.
Neither administration of particular medicines nor infection of other hepatitis viruses were thought to be related to the elimination of HBsAg.
We considered that the patient was healed spontaneously.

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A case of acute excerbation of latent autoimmune hepatitis with severe jaundice after treatment of recombinant human alpha-interferon

Hiroshi WATANABE, Masahiko TANAKA, Hiroyuki KUSUHARA, Hiroshi SHIJO, M ...

1993 Volume 34 Issue 3 Pages 234-238
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.234

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A 54-year-old man with latent autoimmune hepatitis who presented acute excerbation with severe jaundice following the treatment of recombinant alpha interferon (α-IFN) is reported. s-GPT and bilirubin increased after the initiation of treatment which temporaril ydiscontinued after 28 days. Maximum level of s-GPT and total bilirubin attained 1278IU/l and 29.3mg/dl respectively, in spite of the discontinuance of α-IFN administration. s-GPT and bilirubin subsequently showed rapid decreases, and histological findings after the pause in treatment were worse than those obtained before treatment. And then, autoantibodies turned positive after the pause in treatment. The cause of these hepatic disorders induced by IFN administration is not clear, we think that the latent autoimmune hepatitis was influenced by the IFN. The present case suggests that studies regarding the complications of treatment with IFN are necessary.

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A case of post-infantile giant cell hepatitis with positive of LE cell, antinuclear antibody

Kiyomasa KOBAYASHI, Etsuko HASHIMOTO, Makiko TANIAI, Urara WATANABE, H ...

1993 Volume 34 Issue 3 Pages 239-243
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.239

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A 41-year-old woman was admitted to our hospital with jaundice and general fatigue. Liver function tests revealed elevation of total bilirubin and transaminase with marked gammaglobu- linemia. Serum antinuclear antibody, anti-DNA antibody and LE cell were positive. All viral markers were negative.
Predonisolone, 40mg/day was prescribed and a rapid improvement was observed in transaminase, total bilirubin and gammaglobulin. 141 days later, liver biopsy was performed, which revealed marked multinucleated giant cell infiltration. Multinucleated giant cell transformation is a common histological feature of several types of liver disease in the neonatal period. However, the presence of a number of Multinculeated giant cells in the liver is rare, later in life. It is noteworthy that Post-infantile giant cell hepatitis occurred in association with positive of LE cell, antinuclear antibody.

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A case of ulcerative colitis appeared three years after liver transplantation for primary sclerosing cholangitis

Takeyuki NAKAJIMA, Yasushi SAKANISHI, Tsunehisa KAWASAKI, Teruya YOSHI ...

1993 Volume 34 Issue 3 Pages 244-251
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.244

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A case of Japanese male patient with primary sclerosing cholangitis who had received a liver transplantation in the U.S.A. in 1988 (at age of 16) is reported. He experienced several clinical manifestations scinece he returned to Japan. He had severe back pain just after his return to Japan, and about 1 year later he had pain on walking due to osteonecrosis of the femoral condyle, which were assumed to be side effects of steroids.
This patient has also shown a persistent abnormal liver function tests caused by an anastomotic stricutre at the site of choledochojejunostomy, and ulcerative colitis occurred to this patient 3 years after the transplantation. However, those problems have been successfully managed, and at present, he lives an ordinary life as an university student.

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1993 Volume 34 Issue 3 Pages 252-253
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.252

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1993 Volume 34 Issue 3 Pages 254-255
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.254

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1993 Volume 34 Issue 3 Pages 256-257
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.256

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[title in Japanese]

[in Japanese], [in Japanese]

1993 Volume 34 Issue 3 Pages 258
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.258

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[title in Japanese]

1993 Volume 34 Issue 3 Pages 259-286
Published: March 25, 1993
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.34.259

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