Kanzo

Studies on Liver Alcohol Dehydrogenase Activity and Isozyme of Various Liver Diseases.

Makoto FUJII, Hideo NAGASHIMA, Kiyowo KOSAKA

1977 Volume 18 Issue 4 Pages 231-238
Published: April 25, 1977
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.18.231

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ADH activity in the extract of liver biopsy specimen was measured at pH 8.6 and 10.0 according to Wartburg. Liver ADH activity in chronic hepatitis with sublobular necrosis and liver cirrhosis decreased significantly. In a case of hepatocellular carcinoma ADH level decreased more significantly than in the cases mentioned above. ADH in non-alcoholic fatty liver and alcoholic liver injury, determined at pH 10.0, decreased comparing to normal. An atypical ADH was most frequently detected in alcoholic liver injury and not in controls.
A decreases ADH activity in either Band II or III was observed in chronic hepatitis with sublobular necrosis and liver cirrhosis. In alcoholic liver injuries, an atypical ADH was detected associating with decreased Band II. It is suggested that a certain isozyme pattern of ADH as well as the other enzymes could represent de-differentiation of the liver parenchymal cells since Band I and II of ADH which were detected in fetal and newborn liver, increased in a case of hepatocellular carcinoma.

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Studies on hepatic transport mechanism of organic anions

Influences of some drugs which accerelate the hepatic excretory function on Y and Z proteins.

Tomiko OKA

1977 Volume 18 Issue 4 Pages 239-246
Published: April 25, 1977
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.18.239

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Influences of some drugs on both Y and Z proteins of the rat liver were examined by means of Sephadex G-75 column chromatography. Both proteins were confirmed by means of SDS-polyacrylamide gel electrophoresis. The drugs examined those enable to increase the hepatic excretion of BSP or may improve BSP retention test in the patient with chronic liver disease.
Either sod. phenobarbiturate or polyen phosphatidylcholine increased the amount of Y protein significantly, and liver hydrolysate tended to increase it slightly. None of sod. hippurate, sod. salicylate and, sod. dehydrocholate affected on the amount of Y protein. None of these drugs affected on the amount of Z protein. An administration of large dose ofprednisolone, however, decreased amount of Y and Z proteins. The results suggest that an increase of biliary excretion of BSP dose not always depend on an increased amount of Y protein.

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Studies on hepatic transport mechanism of organic anions

Mechanism of hyperbilirubinemia induced by D-galactosmine: Possible role of hepatic Y protein

Tomiko OKA

1977 Volume 18 Issue 4 Pages 247-255
Published: April 25, 1977
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.18.247

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This study was carried out for the purpos of clarifying the mechanism of transient hyperbilirubinemia caused by D-galactosamine (abbreviated as Gal) in rats.
After 12 hours of an administration of Gal, serum transaminase and serum bilirubin (indirect type dominat) level were elevated slightly. In this stage, the concentration hepatic Y and Z proteins, and the rate of hepatic uptake of bilirubin-H³ were decreased, whereas hepatic UDPGT activity was not affected. Although the reduction of Y protein was seen earlier than the appearance of hyperbilirubinemia, the reduction of hepatic uptake of bilirubin-H³ was coincided with the development of hyperbilirubinemia.
The hyperbilirubinemia and reduction of Y protein caused by Gal was protected with the pretreatment of phenobarbital or 3-methylcholanthrene.
The results suggest that the transient hyperbilirubinemia caused by Gal was initiated by the disturbed function of the hepatic plasma membrane rather than by the decreased Y protein which bind bilirubin preferably, because some authors pointed out the injury of hepatic plasma membrane as a primary lesion which was induced by Gal.

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Effect of Phenobarbital on Experimenally induced Intrahepatic Cholestasis

Hirohiko ABE, Toyoaki MAEYAMA, Kyuichi TANIKAWA

1977 Volume 18 Issue 4 Pages 256-265
Published: April 25, 1977
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.18.256

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Experimental studies were carried out to elucidate the effect of phenobarbital on intrahepatic cholestasis.
Cholestasis was experimentally induced in Wistar male rats by the administration of alphaNaphthylisothiocyanate (ANIT) or Taurolithocholate (TLCA).
In the phenobarbital pretreated rats, serum bilirubin level was lower and bile flow was increased at the 2nd day after the administration of ANIT, and furthermore, clearance of BSP was also accelerated in comparison with the one of non-phenobarbital treated rats. In TLCA induced cholestasis, bile flow and bilirubin concentration in bile were increased, serum bilirubin level was lower, and furthermore, excretion of BSP into bile was increased and clearance was also accelerated by phenobarbital pretreatment.

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Corticosteroid therapy in chronic active hepatitis

Hironaka KAWASAKI, Katsuhiko ARIMURA, Tomofumi OGATA, Toshitake IRISA, ...

1977 Volume 18 Issue 4 Pages 266-273
Published: April 25, 1977
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.18.266

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A controlled trial of corticosteroid treatment was performed in two randomly selected groups of patients with chronic active hepatitis during one year. The corticosteroid group contained 24 and the control group 20 patients. When compared with the control group, corticosteroid therapy caused a resolution of biochemical abnormalities in which the most significant finding was a marked decrease in serum gamma globulin and GOT. Galactose and unconjugated bilirubin clearances were significantly improved, but tolbutamide tolerance did not change. Histological features, such as liver cell degeneration, Kupffer cell mobilization and cell infiltration in portal tracts were also improved. It is concluded that a resolution of biochemical, metabolic and histological abnormalities after corticosteroid therapy may be attributable to decreased hepatic mesenchymal reaction and increased hepatic parenchymal cell function.

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Acute hepatitis associated with hemolytic anemia, elliptocytosis and neutropenia

Yoshio MIZUNO, Hiromasa ISHII, Minoru YAMAMOTO, Keisuke TOYAMA, Masaha ...

1977 Volume 18 Issue 4 Pages 274-280
Published: April 25, 1977
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.18.274

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The hematological abnormalities can frequently be demonstrated in chronic liver diseases. But acute hemolytic anemia has rarely been noted during the course of acute viral hepatitis.
We reported a case of 33 year-old female patient with acute hepatitis that was concomitantly associated with acute hemolytic anemia and elliptocytosis. The number of erythrocytes to be included in Günther's groups III and IV together counts up to 54% in the most severe hemolytic state. Ten weeks after the onset of acute hepatitis she complained of mild fever and sore throat. Blood examination at this time revealed a leukocytopenia mostly due to a decrease of neutrophils. In addition to the case presentation, the paper deals with a review of the hematological abnormalities observed in acute hepatitis.

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A case of liver damage caused by oral contraceptive agent (Anovlar)

Ryohmi SASAKI, Yasuyuki ARAKAWA, Tokumichi KATSUHARA, Hitoshi KUWANA, ...

1977 Volume 18 Issue 4 Pages 281-287
Published: April 25, 1977
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.18.281

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A 34-year-old woman had pruritus and severe jaundice during the first cycle of treatment with Anovlar, a mixture of 4mg norethinsterone acetate as the progestogen and 0.05mg ethinvloestradiol as the estrogen. She had no history of exposure to other hepatotoxic agents and recurrent jaundice of pregnancy. The laboratory data included a elevated level of serum bilirubin and a slight increase of alkaline phosphatase activity. But other liver-function tests were normal except for BSP excretion. Drip intravenous cholangiography revealed no extrahepatic biliary obstruction. The clinical and biochemical findings suggested intrahepatic cholestatic jaundice, which disappeared within sixty days after withdrawal of the drug. A liver biopsy specimen showed intracellular bile stasis in the centrilobular zone and also bile thrombi in the dilated bile canaliculi. There were a scanty portal inflammation and the absence of proliferation of bile ducts and fibroblasts. The lymphoblastic transformation test to Anovlar was negative (below 5%). This suggests that Anovlar may exert a hepatotoxic effect.

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1977 Volume 18 Issue 4 Pages 288
Published: April 25, 1977
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.18.288

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1977 Volume 18 Issue 4 Pages 289
Published: April 25, 1977
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.18.289

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1977 Volume 18 Issue 4 Pages 290
Published: April 25, 1977
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.18.290

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

1977 Volume 18 Issue 4 Pages 291
Published: April 25, 1977
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.18.291

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[title in Japanese]

1977 Volume 18 Issue 4 Pages 292-300
Published: April 25, 1977
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.18.292

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