The present study was undertaken to examine biochemically and histologically the relationship between tissue repair and collagen formation after gingivectomy. Two biopsy samples of labial gingival tissue were taken from the same area of each patient with periodontal disease at three times; before, one month and six months after surgery. The neutral salt-soluble and -insoluble collagen content in these biopsy samples was assayed colorimetrically, while a parameter for evaluation of the collagen fibers in 5μ histologic sections stained with Masson-Goldner was expressed in term of numerical density under the multispectrophotometer.
The results were as follows;
1. The amount of soluble collagen extracted 3 times every 24 hours decreased with time.
2. The soluble collagen content before surgery averaged 47.66×10^-2μg/mg (gingival wet weight), and it increased to 68.66×10^-2μg/mg at one month after surgery, and followed by a slight decrease to 60.82×10^-2μg/mg at six months after surgery. This may reflex a more active collagen synthesis in the gingival tissue at one month after surgery.
3. The mean preoperative value of insoluble collagen was 71.83μg/mg and at one month after surgery the amount decreased to 32.83μg/mg, less than half of preoperative value, and then at six months after surgery it returned to 68.49μg/mg, approximately preoperative value.. This may show that the collagen maturation in the gingival tissue repair has completed by six months after surgery.
4. In the present study a close correlation was demonstrated between the insoluble collagen content and the numerical density of collagen fibers in the histologic sections.
5. In the histologic sections before and six months after surgery the collagen fibers were more dense in oral epithelium site than pocket epithelium site, whereas they were found loosely throughout gingival lamina propria of one month postoperative sections. It may be related to the degree of the inflammatory cell infiltration and the amount of mature collagen that the distribution of collagen fibers before and six months after surgery was different from one month after surgery.

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This study was made for the first step to clear how occlusal trauma and periodontal inflammation influence the periodontal destruction. Two adult male Macaca irus monkeys were used for this experiment. Monkey A was accelerated the gingival inflammation to be fed soft food with 20% sucrose. The other hand, monkey B was fed hard food and was brushed the teeth and gingiva every 2 days. At the beginning of the experiment, the clinical crowns of both animals' left lower first molars were cut off, cast gold high crowns which extended mesially were set on the left lower secod molars. After 14 days the same crowns were set on the right lower second molars in the same manner. Every 7 days the animals were clinically examined about gingival conditions. 28 days after setting the first crown, the animals were sacrificed and tissue sections were prepared for histological examination.
The results were as follows.
1. Gingival index, gingival exudate, plague score and pocket depth of monkey A were higher than them of monkey B.
2. The test teeth were depressed and functional occlusal plane was reestablished in 2 or 3 weeks after setting high crowns.
3. By occlusal trauma, the mesial and furcal periodontal tissues of the high crowned teeth were destructed. Occlusal trauma strongly affected furcation area.
Not only alveolar bone but also cementum and dentin of the furcation area were extensively destructed.
4. Nevertheless the test teeth were affected by occlusal trauma, inflammatory cells stayed colonaly to the transseptal fibers and didnot spread to the periodontal ligament area. So the degree of periodontal destruction was not defferent between the inflammed and normal animals.

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Gingival Crevicular Fluid was sampled from 8 patients with the mild periodontitis. The filter paper strips (Harco Electronics Ltd, Winnipeg, Canada) were inserted in the periodontal pocket with intracrevicular method for 5min. The paper strips was quantitated by Harco-Gingival Crevicular Fluid Meter (GCFM, Harco Electronics Ltd, Winnipeg, Canada).
The depth of the periodontal pocket were measured on the labial site of upper or lower incisor teeth.
The degree of gingival inflammation was according to the criteria of the Gingival Inflammation by Löe and Silness (1963). The examinations were made by one of authors.
The standardised oral color-photographs were taken and the variation in gingival-schrinkage was measured with Nikon Profile Projector (Model V-16C, NIPPON KOGAKU, Japan)
Eight patients were treated with brushing and scaling. Each examination was performed on the first examination, 4 weeks and 8 weeks.
The results obtained were as follows.
1) Gingival Crevicular Fluid was much more influenced by the degree of gingival inflammation than by pocket depth. But, variation in gingival schrinkage was not seen to influence the amount of Gingival Crevicular Fluid.
2) A new Gingival Crevicular Fluid Meter-PERIOTRON has been deviced for the measurement of gingival crevicular fluid. This newly -developed Gingival Crevicular Fluid Meter electrically could determine the amount of gingival crevicular fluid. Therefore, this is capable of accurately measuring the fluid on the paper strips. Further, this device shows to be quickly, easy, precisely, automatical digital read out and useful.

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In the present study the authors were concerned with the effect of Lysozyme-containing chewing gum on the chronic marginal periodontitis.
Two kinds of chewing gums (with Lysozyme and without Lysozyme) were selected for the experiment. Each experimental group was composed of 35 individuals aged 19-53 years.
They were instructed to chew the gums for five minutes after each breakfast, lunch and dinner.
As a result of the study, the authors arrived at the following conclusions.
1. Reddening, Swelling: After three weeks a statistically significant difference(P<0.05) was seen between the Lysozyme group and placebo group.
2. Bleeding: After one weeks a statistically significant differnce (P<0.01) was seen beteen the Lysozyme group and placebo group.
3. Pus discharge: After three weeks a statistically significant difference (P<0.05) was seen btween the Lysozyme group and placebo group.
4. Pocket depth, Dental plaque: After three weeks a statistically significant difference was no appreceable difference in the effect between Lysozyme group and placebo group.
5. No adverse effect was observed in the administration of Lysozyme-containing chewing gum at all

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