巻号一覧
40 巻, 3 号
選択された号の論文の17件中1～17を表示しています
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YUKITO OOTA, YUKIO YAMAMOTO, RYOHEI FUJII1953 年 40 巻 3 号 p. 187-203
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリー1. Dehydrogenase activities of tissue extracts of embryonic organs of a legume, Vigna sesquipedalis, germinated and grown in the dark wholly at the expense of its own reserve foodstuffs were investigated by the Thunberg method.
2. Of 19 compounds tested, 10, including every component acids of the TCA-cycle, i.e. pyruvic, citric, α-ketoglutaric, succinic and malic acids, were oxidized under the anaerobic conditions by each anabolic organs investigated, namely, plumules, hypocotyls, and radicles. On the other hand, the catabolic organ, cotyledon, was found to have the same dehydrogenase pattern as observed in the anabolic ones but for the absence of citric dehydrogenase, the only TPN-linked enzyme in the TCA-cycle.
3. TPN was detected in all anabolic organs tested but not in the catabolic one, while DPN was found to be distributed ubiquitously in the embryonic tissues.
4. Addition of either TPN or ATP to crude protein from cotyledon could evoke the citric dehydrogenase action. In the case of ATP ex-periment, it was required to incubate previously the preparation for a short time with ATP so as to induce the enzyme activity. DPN preexisting in the protein preparations was presumably to be phosphory-lated into TPN in this incubation period.
5. Every dehydrogenase activities of embryonic tissues expressed on a dry weight basis rose rapidly as the seed germinated and most of them soon passed their respective peaks. However, the protein con-tents in a unit dry weight of the preparations showed little change in the germination stage. Thus the enzyme activations have practically not to involve syntheses of new enzyme proteins, but probably hydrolytic conversion of some inert proteins into active ones.
6. Production of both apoenzyme and coenzyme of malic de-hydrogenase was inhibited by 2, 4-DNP. This uncoupling agent affected the process of coenzyme activation more strikingly than that of apo-enzyme activation. Hence, aerobiosis is thought to be more necessary conditions for the activation of coenzyme than for that of apoenzyme.
The authors are indebted to Prof. T. Mori of this institute, and members of the research group on ‘Development and Differentiation in Higher Plants’ (representative research worker: Dr. S. Yamaguchi) for their sustained interest and helpful criticism during the course of this study. Thanks are also due to Mr. T. Fukuo and Miss Y. 0da for their technical assistance.抄録全体を表示PDF形式でダウンロード (1069K) -
KOOZOO KAZIRO, GORO KIKUCHI, TAKASHI OGAWA, MASATARO YAMADA1953 年 40 巻 3 号 p. 205-225
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーA variety of experiments were carried out in order to analyze the chemical mechanism of choleglobin formation in the system of hemo-globin, ascorbic acid and oxygen. The results of the experiments are summarized as follows:
1. By treating the reaction solution with NaOH and Na2S2O4, the choleheme protein is converted to alkali denatured cholehemo-chrome which can be determined by means of spectrophotometry. The method enables us to follow up the present reaction process quantitative-ly and with fair exactness.
2. From the optical density of alkali denatured cholehemochrome at the wavelength of 618μp as experimentally determined, εmol. for choleheme is calculated as 1.36×104.
3. The structural change which occurred in the heme part of choleglobin is just the same as in that of cholehemochromogen.
4. During the process of choleheme formation in the reaction system without catalase, almost 20 moles of ascorbic acid are used up per mole of protoheme.
5. The reaction curves of choleheme production and ascorbate oxidation are in parallelism. Both of the curves are characteristic in their S-shaped type. The slope of the S-shape is pronounced either with the decrease of ascorbate concentration or by the presence of catalase or further with the lowering of the temperature.
6. Choleglobin can be obtained from hemoglobin by the direct action of H2O2 upon the latter and without O2. This reaction can proceed whether ascorbate is present or not so far as the hemoglobin is kept in its Fe++ state. Methemoglobin (Fe+++), however, can not produce choleglobin by the same oxidant.
7. In the system of methemoglobin, ascorbic acid and O2, methemoglobin must be first reduced to hemoglobin in order to be oxidized to choleglobin.
8. Choleglobin seems to be able to liberate iron completely by the treatment with 0.4 per cent HCl as well as by the treatment with trichloracetic acid in the deproteinization procedure.
9. Denaturation of globin part have, most likely, some important influence upon the disintegration of heme part.
From the above results, it may be concluded as follows: choleglobin is formed from hemoglobin by the action of H2O2 produced from ascorbic acid and O2 in which the heme molecule itself serves as a peroxidative substrate for its own molecule. It is further suggested that in this process some sort of intermediate interposes, which must be highly active as an oxidative catalyst. Finally, a hypothetical scheme of the reaction process of choleglobin formation is presented by assuming an active role of this probable intermediate.抄録全体を表示PDF形式でダウンロード (1288K) -
YOSHIKZU SAHASHI, KIICHI IWAMOTO, MASAYUKI MIKATA, AKIHIKO NAKAYAMA, H ...1953 年 40 巻 3 号 p. 227-244
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (2429K) -
YOSHIKAZU SAHASHI, KIICHI IWAMOTO, JUNZO HAYASHI1953 年 40 巻 3 号 p. 245-249
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (266K) -
ON THE ENZYMATIC HYDROLYSIS OF CEREBRONYASUHIKO FUJINO1953 年 40 巻 3 号 p. 251-255
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーCerebron being decomposed enzymatically under adequate conditions, galactose was liberated completely from the cerebron molecule and the formation of cerebronylsphingosine was recognized, whereas fatty acid was not liberated. An assumption may be presented there-fore that the enzymatic hydrolysis of cerebron proceeds along the following scheme:抄録全体を表示PDF形式でダウンロード (301K) -
II. MERCURY, CADMIUM OR ZINC, CO-FACTOR OF L-HISTIDINE DEAMINASEMASAMI SUDA, KIYOSHI TOMIHATA, AKIRA NAKAYA, AKIRA KATO1953 年 40 巻 3 号 p. 257-260
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリー1. L-Histidine deaminase was highly purified and completely freed of urocanic acid-decomposing enzyme.
2. Hg++, Cd++ or Zn++ participates the enzymatic reaction as a co-factor.抄録全体を表示PDF形式でダウンロード (235K) -
II. THE ACTION OF CARBOXYPEPTIDASE ON a-BROMOISOBUTYRYL-o-, -m- NAD-p-AMINOBENZOIC ACIDSTAKEO SAITO, KEIICHI SAITO1953 年 40 巻 3 号 p. 261-263
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーThe action of carboxypeptidase on a-bromoisobutyryl-o-, -m- and -p-aminobenzoic acids was studied and a comparison was made of the cleavability of these substances with that of other halogen acyl com-pounds reported by Utsunomiya.
1. Only m- and p-compounds were split by carboxypeptidase as in the case of other halogen acyl derivatives.
2. The optimum pH lies in both cases at pH 8.4.
3. a-Bromo-isobutyryl derivatives are attacked by the enzyme with slowness as compared with other halogen acyl compounds and at nearly the same rate as a-bromo-isocapronyl derivatives.
In conclusion the authors wish to express their sincere thanks to Prof. Dr. T. Uchino for his kind suggestion throughout this work.
The expence of this research has been defrayed from the Scientific Research Ex-penditure of the Department of Education. (T. Uchino)抄録全体を表示PDF形式でダウンロード (152K) -
III. THE EFFECT OF THE ADDITION OF VARIOUS SALTS ON CARBOXYPEPTIDASETAKEO SAITO1953 年 40 巻 3 号 p. 265-271
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーThe effects of various aslts on the hydrolysis rate of choracetyl
TABLE IV Effect of KCN, K4Fe(CN)6, K3Fe(CN)6, FeSO4(NH4)2SO4, NH4Fe(SO4)2and Na_Fe(CN)5NO on the Carboxypeptidase Activity
tyrosine by means of carboxypeptidase was examined in relation to the initial rate of hydrolysis as well as to the optimum pH for the enzyme action.
1. NaCl, KCl, NaF, MgCl2, CaCl2, BaCl2 and AiCl3 exert no considerable influence on the action of carboxypeptidase.
2. Cobalt, nickel, ferrous and ferric salts accelerate the carboxv-peptidase activity, the promoting effect being found to diminish in the following order: Co++>Ni++>Fe++>Fe+++. Manganese salt exhibits no influence on the enzyme.
3. Carboxypeptidase undergoes considerable inhibition by means of silver and mercury salts, while it is entirely unaffected by copper, zinc and lead salts.
4. KCN causes a marked inhibition of carboxypeptidase.
TABLE V Influence of KMnO4 and H2O2 on the Carboxypeptidase Activity
5. Carboxypeptidase is accelerated by most of the iron complex salts tested, except in the case of ammonium ferricalum, which is found to have no effect.
6. Potassium permanganate and hydrogen peroxide exercise no effect on the enzyme.
7. All the salts tested caused no shift of optimum pH for the action of carboxypeptidase.
In conclusion the author wishes to express his sincere thanks to Prof. Dr. T. Uchino for his kind guidance throughout this research.
The expence of this research has been defrayed from the Scientific Research Ex-penditure of the Department of Eeucation.抄録全体を表示PDF形式でダウンロード (390K) -
KIYOO SATOH1953 年 40 巻 3 号 p. 273-275
発行日: 1953年
公開日: 2008/11/18
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TOMOICHI KUSUNOKI1953 年 40 巻 3 号 p. 277-285
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリー1. Both bovine serum albumin and γ-globulin did not bind T. B. and M. B. at high concentrations of the dyes. After alkali-treatment of these proteins, only denatured γ-globulin bound T. B. and M. B. remarkably resulting in the depolymerization of the dyes.
2. R. H. was bound by alkali-treated γ-globulin but not by albumin at the concentrations of the dye where it obeyed the Beer's law.
3. B. P. B.-binding ability of native serum albumin was lost by alkali-treatment of the protein, while, in the case of γ-globulin, the dye-binding ability appeared only after alkali-treatment.
4. Bence-J ones protein prepared from urine of a myeloma patient had the ability to bind R. H., and this ability decreased after heat-treatment, while its solubility did not change at all. The author expresses his thanks to Prof. N. Shimazono for his support and advice, and also to Dr. H. Hirai and K. Shimao for their help throughout these investigations.抄録全体を表示PDF形式でダウンロード (504K) -
I. THE EFFECT OF FATTY ACID ON THE EXCRETION OF XANTHURENIC ACID, AND ITS RELATION TO PYRIDOXINEYAMITO KOTAKE JR., TOSHIRO INADA1953 年 40 巻 3 号 p. 287-289
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリー1. When Na-salts of fatty acids were administered to rat together
TABLE II The Effect of Fatty Acid Salt in the Presence of Pyridoxine
with tryptophan the excretion of xanthurenic acid into urine was greatly increased.
2. This fact should be ascribed to the used-up of pyridoxine for the oxidation of fatty acid, as the injection of pyridoxine checked such an increase. We wish to thank heartilyu to Prof. Yashiro Kotake, for his advice and eccouragement to this research.抄録全体を表示PDF形式でダウンロード (163K) -
II. PRELIMINARY REPORT ON XANTHURENIC ACID DIABETESYAHITO KOTAKE JR., TOSHIRO INADA1953 年 40 巻 3 号 p. 291-294
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーThough it is necessary of further precise experiments to get the final conclusion yet the findings so far attained such as changes of pancreas, the occurrence of cataract and hyperglycemia may justify the conclusion that xanthurenic acid is responsible for the production of pancreatic diabetes. The structual similarity of xanthurenic acid to oxine, which is proved by 0kamoto to be diabetogenic, also favour the above postulation. Since xanthurenic acid, however, is more common metabolites in the living body than alloxan or oxine, we think that our finding give s an important clue to the etiology of human diabetes.
We wish to express our deep gratitude to Dr. Yashiro Ko take who has given very kind guidance in carrying out this research.抄録全体を表示PDF形式でダウンロード (175K) -
III. XANTHURENIC ACID IN THE URINE OF DIABETIC PATIENTYAHITO KOTAKE JR., SEIKI TANI1953 年 40 巻 3 号 p. 295-298
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーThe existence of xanthurenic acid and 3-OH-kynurenine in the urine of diabetes patient were confirmed. Taking into consideration the fact that xanthurenic acid causes diabetic symptoms in white rat, it will be postulated that xanthurenic acid also is a most plausible agent in pathogenesis of human diabetes.
We thank very much to Dr. Yashiro Kotake who encouraged and adviced us throughout this experiment and also to Dr. Takeo Sakan who was kind enough to donate us synthetic xanthurenic acid and 3-OH-kynurenine.抄録全体を表示PDF形式でダウンロード (210K) -
1953 年 40 巻 3 号 p. e1a
発行日: 1953年
公開日: 2008/11/18
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1953 年 40 巻 3 号 p. e1b
発行日: 1953年
公開日: 2008/11/18
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1953 年 40 巻 3 号 p. e1c
発行日: 1953年
公開日: 2008/11/18
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1953 年 40 巻 3 号 p. e1d
発行日: 1953年
公開日: 2008/11/18
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