巻号一覧
73 巻, 6 号
選択された号の論文の24件中1～24を表示しています
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Munekazu SHIGEKAWA, Yuji TONOMURA1973 年 73 巻 6 号 p. 1135-1148
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーThe effects of tropomyosin and the three components of troponin on the Mg2+-activated ATPase [EC 3.6.1.3] activity of reconstituted actomyosin were studied over a wide range of MgATP concentrations (0.002-1mM) in 40mM KCl at pH 7.2 and 25°C. The following results were obtained.
1. In the absence of tropomyosin, each of the two components of troponin with molecular weights of 23, 000 (TN-I) and 37, 000 (TN-P) activated the ATPase activity of actomyosin at MgATP concentrations above 0.1mM and inhibited it below 0.1mM. A mixture of both the components activated the ATPase activity considerably at MgATP concentrations above 0.04-0.05mM and inhibited it slightly below 0.04mM. These effects on ATPase were unaffected by Ca2+. The component of troponin with a molecular weight of 19, 000 (TN-C) neutralized both the activating and inhibitory activities of TN-I, TN-P or a mixture of these two components, regardless of the Ca2+ concentration, although TN-C alone had no effect on the ATPase activity of actomyosin.
3. In the presence of tropomyosin, TN-I inhibited the ATPase activity irrespective of the Ca2+ concentration, so that it reduced the concentration at which MgATP became inhibitory for the ATPase, to the level obtained with the complete relaxing protein system in the absence of Ca2+, while TN-C had no effect on the ATPase activity even in the presence of tropomyosin. In the presence of tropomyosin, TN-P slightly inhibited the ATPase activity at low concentrations of MgATP but activated it at high concentrations of MgATP.
4. When TN-C was added to the TN-I-tropomyosin system, the Ca2+-sensitivity of the ATPase of actomyosin was only incompletely restored, since the inhibitory effect of the TN-C-TN-I-tropomyosin system in the absence of Ca2+ was weak, and since the ATPase was not activated in the presence of Ca2+ and at high concentrations of MgATP. The inhibitory activity of the TN-I-tropomyosin system was unaffected by addition of TN-P. When the TN-P-TN-C-tropomyosin system was added to actomyosin, Ca2+-sensitivity of the ATPase was also observed, but the ATPase activity at high MgATP concentrations was higher in the absence of Ca2+ than in its presence.
5. The relaxing protein activities were completely reproduced, only when tropomyosin and all the three components of troponin were added to actomyosin.抄録全体を表示PDF形式でダウンロード (2355K) -
VI. Changes Induced by Valine in the Branched Chain Amino Acid Transport Systems of Escherichia coliYasuhiro ANRAKU, Tohoru NARAKI, Shigeko KANZAKI1973 年 73 巻 6 号 p. 1149-1161
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーThe properties of branched chain amino acid transport systems in Escherichia coli strains W3092 and WV-5 have been described. W3092 is valine-sensitive and WV-5 is valine-resistant.
Various compounds which are structurally related to branched chain amino acids were studied for their abilities to inhibit the growth of cells and to inhibit the transport activity. Among them, DL-α-hydrazinoisovaleric acid was found to strongly inhibit the growth of both strains. The compound, however, did not affect branched chain amino acid transport activities.
Acetohydroxy acid synthetase activity was measured under various conditions. In both strains, the enzyme was repressed by valine and leucine and showed similar sensitivity to inhibition by valine.
The valine, isoleucine, and leucine transport systems of these strains were characterized as having two different Km values characteristic of the entry of the respective amino acids. All the transport systems showed negative cooperativity.
The change induced by valine in the transport systems were analyzed circumstantially. Valine and leucine greatly reduced the level of binding protein specific for branched chain amino acids when the amino acids were added to cultures of both strains. This suggests that valine acted as an effector of the transport systems, altering their Km and Vmax values, and their Hill coefficients.
Pollock's view of physiological efficiency was introduced in considering the changes effected by valine. Based on this evaluation, the physiological role and regulation of the transport systems are discussed. The mechanism and regulation of active transport systems for amino acids seem to be directed to retaining amino acids in the cell and maintaining their concentrations against a concentration gradient.抄録全体を表示PDF形式でダウンロード (1850K) -
Tomitake TSUKIHARA, Takashi YAMANE, Nobuo TANAKA, Tamaichi ASHIDA, Mas ...1973 年 73 巻 6 号 p. 1163-1167
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーIt was found that crystalline bonito heart ferrocytochrome c was oxidized without significant change of the crystal lattice. The difference. Fourier syntheses between oxidized and reduced crystals were calculated at 4 and 3 Å resolutions. The Fourier maps show that the ferricytochrome c obtained here captures a sulfate anion in the vicinity of the side chains of Lys (99) and Glu (61). The anion binding was anticipated on the basis of biochemical experiments. In other regions, no significant structural change was observed, though on oxidation in solution some drastic conformational changes have been reported. This fact suggests that the mechanism of oxidation in the crystalline state may be different from that in solution.抄録全体を表示PDF形式でダウンロード (710K) -
Takashi MANABE, Hiroyuki HATANO, Keitaro HIROMI1973 年 73 巻 6 号 p. 1169-1174
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリー1. In our preliminary study of the aerobic oxidation of reduced ceruloplasmin, anew intermediate species with an absorption maximum around 420 nm was detected. This paper deals with the kinetics of aerobic oxidation of ceruloplasmin at 25°C and pH 5.5 as determined by investigating the spectral changes during the reaction, the effects of oxygen concentration on the rate constants at 610, 420, and 330nm, and the effect of azide.
2. The time courses of the absorbance changes at 610 and 420nm were found to be biphasic. The rate constants of the first (faster) phase at the two wavelengths were dependent on oxygen concentration, whereas those of the second (slower) phase were both constant at various oxygen concentrations.
3. Azide did not reduce the first phase at 610 and 420 nm very much, but it reduced the rate constants of the second phase at both wavelengths. The second phase at 610 and 420nm was inhibited by azide to the same extent in azide concentrations from 15 to 240μm.
4. The nature of the component responsible for the 420 nm absorption is discussed in the light of these results.抄録全体を表示PDF形式でダウンロード (1015K) -
III. Inactivation of α-Chymotrypsin by p-Diazo-N-acetyl-L-phenylalanine Methyl EsterNobuo YOSHIDA, Makoto YAMASAKI, Shin-ichi ISHII1973 年 73 巻 6 号 p. 1175-1183
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーThe diazo compound derived from Nα-acetyl-L-phenylalanine methyl ester, DAPM (p-diazo-N-acetyl-L-phenylalanine methyl ester), has been synthesized and tested as an active site-directed irreversible inhibitor of α-chymotrypsin [EC 3. 4. 4. 5]. DAPM at 50-fold molar excess causes 60% inhibition of α-chymotrypsin (2×10-5M) in about 5hr at pH 8.0 and 4°C.β-Phenylpropionic acid completely protects α-chymotrypsin from inactivation by this reagent, indicating that azo-coupling occurs probably at or near the active center.
From the results of an efficiency assay using specific substrates and all-or-none assay using cinnamoylimidazole, the remaining activity of partially inactivated enzyme was shown to depend only on the active enzyme molecules present in the preparation, whose active sites could not be modified with the diazo reagent. Amino acid analysis and spectral analysis of the modified chymotrypsin indicate that azotization by DAPM occurs only in tyrosine and lysine residues, and not in histidine, methionine, and serine residues which have been reported to be present in the active site of this enzyme.
The reactivity toward azotization of tyrosine-146, the carboxyl terminal residue of the B chain of α-chymotrypsin, by DAPM was also examined. The results showed that either in the presence of a reversible competitive inhibitor or in its absence, tyrosine-146 is more readily accessible to attack by the reagent as compared with not only the three other tyrosine residues but also any other amino acid residues in α-chymotrypsin.
However, further study is required to identify the amino acid residue modified with DAPM, which might be the site of inactivation of α-chymotrypsin by attack of the reagent and be involved in the formation of the specificity-determining site of the enzyme.抄録全体を表示PDF形式でダウンロード (1510K) -
Isamu SHIIO, Ryuichi MIYAJIMA, Nobukazu KASHIMA1973 年 73 巻 6 号 p. 1185-1193
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーThe effect of Na+ on threonine transport into cells of Brevibacterium flavum was examined. Threonine transport scarcely occurred in the absence of Na+, and was strongly stimulated by the addition of Nat, or required Na+. Li+ showed about half as much stimulatory effect as Na+, whereas no other alkaline metal ions tested showed any stimulatory effect, or they were slightly inhibitory. Na+ stimulated not only the transport of threonine but also that of serine. The stimulatory effect of Na+ on threonine transport was observed not only in gram-positive bacteria such as B. flavum and Micrococcus glutamicus, but also in gram-negative ones such as Aerobacter cloacae, Serratia marcescens and Pseudomonas aeruginosa. The rate of threonine uptake depended on Na+ concentration, while the maximum amounts of uptake were almost identical, independently of the concentration of Na+. Double reciprocal plots of the rate of Na+-dependent threonine uptake against threonine (or Na+) at several fixed levels of Na+ (or threonine) gave straight lines that met at a point on the abscissa, suggesting that the mechanism for this transport system may be sequential and “ordered” or “rapid equilibrium random.” Km values for threonine and Na+ were found to be 3.6×10-5m and 3.7×10-4M, respectively. The uptake of threonine-14C was strongly inhibited by L-serine, L-allo-threonine, and L-threonine methyl ester, to the same degree as by non-labelled threonine. The inhibition by serine was competitive with respect to threonine and non-competitive to Na+. The transport system did not require any exogenous energy source, and was not inhibited significantly by so-called respiratory inhibitors. However, 10-6M gramicidin showed90% inhibition.抄録全体を表示PDF形式でダウンロード (1379K) -
Hiromichi OKUDA, Setsuro FUJII1973 年 73 巻 6 号 p. 1195-1203
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーRat liver esterase [EC 3. 1. 1. 1] was purified to yield an electrophoretically homogeneous component. Anti-liver esterase antibody was prepared using this purified preparation. The antibody effectively precipitated the lipase [EC 3. 1. 1. 3] and esterase activities of adipose tissue lipase fraction, and the esterase activity of the adipose tissue esterase fraction. Based on these and previous results, it is suggested that adipose tissue lipase is composed of esterase and lipid.
Anti-liver esterase antibody also precipitated both hormone-sensitive lipase and lipoprotein lipase of adipose tissue. The lipolytic activity of hormone-sensitive lipase was maximum at pH 6.8 in the absence of serum and at pH 8.0 in the presence of serum. The activity was markedly activated by serum, which stimulated the formation of an enzyme-substrate complex. In the presence of 1M NaCl, serum did not activate lipase activity and the maximum activity remained at pH 6.8. The lipoprotein lipase activity was clearly inactivated by acetone treatment. From these results, it is suggested that hormone-sensitive lipase and lipoprotein lipase may be the same entity.抄録全体を表示PDF形式でダウンロード (3327K) -
Shin-ichi ABE, Koscak MARUYAMA1973 年 73 巻 6 号 p. 1205-1210
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーThe dynamic elastic modulus of α-actinin-F-actin complex was measured under various conditions. It increased up to a ratio of 30-40% a-actinin by weight to F-actin. The elastic modulus of α-actinin-F-actin complex decreased with increasing amplitude of oscillation during the measurements. The elastic modulus was, however, markedly increased by sonic vibration, which would disperse huge aggregates of the complex. The value of the elastic modulus was very large at low temperatures (5-10°C) and very low at 40°C, where dissociation of the complex took place. Tropomyosin decreased the dynamic elastic modulus of α-actinin-F-actin complex. Based on the present observations, a scheme for the network of the α-actinin-F-actin complex is proposed.抄録全体を表示PDF形式でダウンロード (755K) -
Fumio ARISAKA, Masaru KAWAMURA, Koscak MURAYAMA1973 年 73 巻 6 号 p. 1211-1215
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーThe change in the length distribution of F-actin transformed from Mg-polymer was followed in detail. The Poisson-type length distribution changed into an exponential type within 20 min at 45°C, whereas the number-average length (0.4μm) remained almost constant.
By assuming appropriate rate constants, including those for the reverse reactions of F-actin and nuclei formation, the above process was analyzed numerically.抄録全体を表示PDF形式でダウンロード (655K) -
Shintaro IWASHITA, Fujio EGAMI1973 年 73 巻 6 号 p. 1217-1222
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリー1. Using p-nitrophenyl α-D-fucoside as substrate, α-D-fucosidase was purified above 200-fold from Takadiastase by acetone fractionation and chromatographies on DEAESephadex, CM-cellulose, and Sephadex G-200. The purified enzyme was practically free from all other glycosidase activities tested except α-D-galactosidase activity.
2. Experiments showed that the hydrolyses of α-D-fucoside and α-D-galactoside by the purified preparation were due to a single enzyme, which was designated as “α-D-fucoside fucohydrolase, Aspergillus oryzae.”
3. The optimal pH was 4.5-5.0. p-Nitrophenyl α-D-fucoside was hydrolyzed much more rapidly than p-nitrophenyl α-D-galactoside. The Km value for p-nitrophenyl α-D-fucoside was 3.7×10-3M and for p-nitrophyl α-D-galactoside was 7.7×10-3M.
4. The enzyme activity was inhibited by PCMB or AgNO3, but not by EDTA, CuSO4, or ZnSO4 α-D-Fucosidase activity was competitively inhibited by D-fucose (Ki: 7.5mM) or D-galactose (Ki: 12.8mM).
5. The crude extract of Takadiastase contained another α-D-galactosidase [EC 3. 2. 1. 22] with little or no α-D-fucosidase activity.抄録全体を表示PDF形式でダウンロード (852K) -
V. Hydrolyses of Phenyl α-Glucosides and Phenyl α-MaltosidesNobuyuki SUETSUGU, Eiji HIROOKA, Hideki YASUI, Keitaro HIROMI, Sô ...1973 年 73 巻 6 号 p. 1223-1232
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリー1) Evidence was obtained that glucoamylase [EC 3. 2. 1. 3] from Rhizopus delemar catalyzes the hydrolysis of phenyl α-glucoside, although the rate is much lower than that for phenyl α-maltoside.
2) The Michaelis constant (Km) and the molecular activity (k0) were determined at pH 4.5 and 25° for glucoamylase-catalyzed hydrolyses of phenyl a-glucosides with the substituents p-NO2, H, P-CH3, and p-C (CH3) 3, and phenyl α-maltosides with p-NO2, H, p-CH3, P-C2H5, and p-C (CH3) 3.
3) The effect of substituents upon Km was marked for both the glucosides and the maltosides, and there was a slight effect upon k0 for glucosides. No appreciable effect on k0 was observed for maltosides, as was expected.
4) When the values of Km and k0 for a particular glucoside were compared with those of the corresponding maltoside having the same substituent, Km was about 10 times larger and k0 was 500-1, 000 times smaller for glucosides than for maltosides.
5) The remarkable difference in k0 between the glucosides and maltosides, which cannot be explained on the basis of the chemical nature of the bond to be hydrolyzed, was reasonably accounted for in terms of the statistical weight of productive and nonproductive complexes as determined by the subsite affinity of this enzyme (1).抄録全体を表示PDF形式でダウンロード (1671K) -
Morimitsu NISHIKIMI, Yoshimasa KYOGOKU1973 年 73 巻 6 号 p. 1233-1242
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーThe binding of riboflavin, 3-methylriboflavin, and lumiflavin to the apoprotein of egg white flavoprotein was studied by fluorometry, spectrophotometry, and the circular dichroism (CD) technique.
The fluorescence of the flavins was completely quenched upon their binding to the apoprotein, whereas 86% of the fluorescence of the protein was quenched on its binding to riboflavin or 3-methylriboflavin, and 77% on its binding to lumiflavin. The association constants of the flavin-apoprotein binding, obtained from fluorometric titrations, were 5.0×108 M-1 for riboflavin, 4.0×108 M-1 for 3-methylriboflavin, and 2.4×107 M-1 for lumiflavin. The visible absorption spectra of all the flavins were modified on their binding to the apoprotein.
The CD spectra of the riboflavin- and 3-methylriboflavin-apoprotein complexes were similar in the range of wavelengths investigated (200-550nm), but differed from the CD spectrum of the lumiflavin-apoprotein complex.
The difference absorption spectra in the near-ultraviolet region resulting from the binding of the flavins to the apoprotein indicated perturbations of the spectra of both the flavin and aromatic amino acid residues of the protein. These spectral perturbations suggested that the aromatic groups became less accessible to solvent or interacted with the flavin. The comparison of the CD spectra of the flavinapoprotein complexes with the spectrum of the apoprotein also supported this view.
The CD data in the region from 200 to 225 nm indicated that the conformation of the polypeptide backbone of the apoprotein was hardly affected by the binding of the flavins.抄録全体を表示PDF形式でダウンロード (1487K) -
Yoshiko NAKAE, Kiyoshi IKEDA, Kozo HAMAGUCHI1973 年 73 巻 6 号 p. 1249-1257
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーThe effects of succinylation, guanidination and maleylation on the circular dichroic (CD) spectrum of hen egg-white lysozyme [EC 3. 2. 1. 17] were studied. These modifications of the amino groups resulted in changes in the aromatic CD spectrum and in the activity toward glycol chitin. It was suggested that the state of tryptophyl residue (s) was changed by these modifications. The pH dependence between pH 4 and 12 of the ellipticity at 305 nm of the modified preparations was studied in detail. On the alkaline side, the pH dependence curve of the ellipticity at 305 nm, which reflects the ionization of a tyrosyl residue, shifted depending on the modification used. This shift could be well interpreted in terms of the change in the mean net charge of the lysozyme molecule. On the other hand, the pH dependence in the pH range between 7 and 4 of the CD band at 305 nm, which reflects the ionization of Glu-35, was not affected by the mean net charge of the protein molecule. This observation suggests that Glu-35 is located in very special environment and is shielded from the charges present in the rest of the protein molecule.抄録全体を表示PDF形式でダウンロード (1216K) -
Takachika AZUMA, Kozo HAMAGUCHI, Shunsuke MIGITA1973 年 73 巻 6 号 p. 1259-1268
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーThe urea denaturation of Bence Jones proteins, one type k (Ta) and two type λ(Fu and Ni), has been studied by measurements of optical rotation, circular dichroism (CD), ultraviolet absorption, and the reactivities of tryptophyl residues and disulfide bonds. In the case of Ta protein, the changes with urea concentration in the optical rotation at 250 nm, the CD spectrum and the reactivity of the intrachain disulfide bonds toward dithiothreitol occurred in at least two stages and no complete transition was observed even with 8 M urea. On the other hand, both the tryptophyl residues had reacted with 2-hydroxy-5-nitrobenzyl bromide in 4 M urea. The transition curve, measured by ultraviolet absorption spectroscopy, was characterized as sigmoidal. On the basis of these findings and the observation by Titani et al.(J. Biol. Chem., 244, 3521 (1969)) that the disulfide bond in the variable half of a k protein resists reduction, it was suggested that the unfolding of the constant half and that of the variable half occur simultaneously and the intermediate observed in the course of urea denaturation can be characterized as the molecule in which the constant half is completely unfolded and a portion of the variable half which contains a disulfide bond remains folded. The change in the CD spectrum of Ni protein with urea concentration showed that the constant half is less stable than the variable half, but a conformational change in the variable half begins to occur prior to completion of the unfolding of the constant half. In the case of another type λ protein, Fu, there was no distinct difference between the stabilities of the constant and variable halves. For both λ proteins, the reactivity of the intrachain disulfide bonds increased before the changes in the optical rotation and absorption were observed.抄録全体を表示PDF形式でダウンロード (1667K) -
Hiroyuki KONDO, Keiichi KAGOTANI, Mikiko OSHIMA, Makoto ISHIMOTO1973 年 73 巻 6 号 p. 1269-1278
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーTaurine dehydrogenase was solubilized and purified 13-fold from a taurine-decomposing bacterium by ammonium sulfate precipitation and Sepharose 4B gel filtration. The partially purified enzyme did not catalyze the oxidation of taurine by oxygen directly, but did do so in the presence of phenazine methosulfate. The optimal pH was 8.4. Fifty percent of the enzyme activity was lost on incubation at 40° for 20 min. The Km values of the enzyme for taurine and phenazine methosulfate were determined to be 2×10-2M and 5.6×10-5M, respectively. Taurine was the only substrate oxidized among a variety of amines. Nicotinamide nucleotides and flavin nucleotides did not serve as electron acceptors. Theenzyme reaction was inhibited by isonicotinic acid 2-isopropylhydrazide, phenelzine, and p-chloromercuribenzoate. The reaction product was identified by paperchromatography and electrophoresis as sulfoacetaldehyde, and ammonia formation and oxygen consumption occurred stoichiometrically.抄録全体を表示PDF形式でダウンロード (1639K) -
VI. Preparation of Diribonucleoside Monophosphates Containing 4-ThiouridineKazunobu MIURA, Masatoshi SHIGA, Tohru UEDA1973 年 73 巻 6 号 p. 1279-1284
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーA new procedure for the preparation of diribonucleoside monophosphates containing 4-thiouridine was devised by application of the amino-thiol exchange reaction with hydrogen sulfide to the cytidine moiety of several diribonucleoside monophosphates containing cytidine.抄録全体を表示PDF形式でダウンロード (897K) -
Michio TANAKA, Michiaki KOHNO, Ikuo YAMASHINA1973 年 73 巻 6 号 p. 1285-1289
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリー4-L-Aspartylglycosylamine amido hydrolase (Amidase) prepared from hog serum and kidney was studied for its substate specificities with synthetic 4-L-aspartylglycosylamines in which N-acetylglucosamine, N-acetylgalactosamine, galactose, mannose, and glucose are present as the carbohydrate moieties; and with other aspartyl derivativies including aspartylcyclohexylamine, aspartylaniline, asparthydroxamate, asparthydrazide, and asparagine. Except for aspartylcyclohexylamine and aspartylaniline, which served as competitive inhibitors, the compounds tested were degraded by Amidase at various rates. The enzymes from serum and kidney differed in their relative reaction rates and Michaelis constants for each substrate.
Formation of an aspartyl-enzyme as an intermediate was demonstrated by carrying out the enzyme reaction in the presence of hydroxylamine.抄録全体を表示PDF形式でダウンロード (837K) -
II. Further Studies on Substrate Specificity and Mode of ActionMasahiro SUNO, Akihiko NOMURA, Yoshihisa MIZUNO1973 年 73 巻 6 号 p. 1291-1297
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリー1. Hydrolysis of 2'(3')-AMP and 2'(3'), 5'-ADP by potato 3'-nucleotidase-nuclease was studied in detail and the specific attack on nucleoside 3'-phosphates was confirmed.
2. Poly A was degraded principally to 5'-AMP by this enzyme.
3. A time course study of hydrolysis of RNA indicated the endonucleolytic nature of the nuclease.
4. Adenosine 3'-benzylphosphate was hydrolyzed to adenosine and benzylphosphate by this enzyme, while adenosine 5'-benzylphosphate was not a substrate.
5. It is concluded from those findings that the enzyme can recognize nucleoside 3'-phosphate moiety (monoester and diester) as the substrate and cleaves the 3' O-P bond.
6. The kinetic parameters, Km and Vmax, of 3'-nucleotidase-nuclease for 3'-ribonucleotides were determined. Ribonucleosides and 5'-ribonucleotides were competitive inhibitors of this enzyme and their Ki values were determined. RNA and also poly A were potent competitive inhibitors of this enzyme.
7. On the basis of these data, it seemed reasonable to assume that the potato 3'-nucleotidase-nuclease has an active site which includes a nucleoside-binding site and two phosphate-binding sites.抄録全体を表示PDF形式でダウンロード (1020K) -
Fukusaburo HAMADA, Yasumi OHSHIMA, Tadao HORIUCHI1973 年 73 巻 6 号 p. 1299-1302
発行日: 1973/06/25
公開日: 2011/01/25
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Shoji KATSUKI, Kihachi SAITO, Hiroshi YOSHIDA1973 年 73 巻 6 号 p. 1303-1305
発行日: 1973/06/25
公開日: 2011/01/25
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Hiroshi NAGANO, Takashi SHIMADA, Ryoiti SHUKUYA1973 年 73 巻 6 号 p. 1307-1309
発行日: 1973/06/25
公開日: 2011/01/25
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Akio INOUE1973 年 73 巻 6 号 p. 1311-1313
発行日: 1973/06/25
公開日: 2011/01/25
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Sachiko CHIBA, Makoto ISHIMOTO1973 年 73 巻 6 号 p. 1315-1318
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーPDF形式でダウンロード (660K) -
Eiji ISHIKAWA1973 年 73 巻 6 号 p. 1319-1321
発行日: 1973/06/25
公開日: 2011/01/25
ジャーナル フリーPDF形式でダウンロード (544K)
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