巻号一覧
45 巻, 12 号
選択された号の論文の10件中1～10を表示しています
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I. PREPARATION AND CHARACTERIZATIONSHUZO AKASHI, HANAKO YOKOI, HIDEO GOTO1958 年 45 巻 12 号 p. 959-971
発行日: 1958/12/25
公開日: 2008/11/18
ジャーナル フリー1. In a certain species of Alcaligenes faecalis has been found to occur a special kind of nucleic acid referred to as alkaligenic acid. It is a high molecular compound of N 13.7 per cent and P 8.2 per cent, showing considerable homogeneity in electrophoresis.
2. It contains about 8.5 per cent of sugar-polypeptide complex in a bound form which can be liberated by 7 minute-1 N HCl-hydrolysis with simultaneous splitting of inorganic phosphate. The complex includes ribose, glucosarnine and polypeptide, the latter comprising about 10 amino acids.
3. The nucleic acid moiety is composed of both types of nucleic acids, 26.7 per cent of DNA-P and 50.7 per cent of RNA-P of the total P and are characterized by a significantly high content of guanine and a markedly low content of thymine.抄録全体を表示PDF形式でダウンロード (735K) -
II. NEUTRAL SUBSTANCES IN THE URINE OF RABBITS ADMINISTERED (+)-CATECHINYASUYOSHI OSHIMA, HIROYASU WATANABE1958 年 45 巻 12 号 p. 973-977
発行日: 1958/12/25
公開日: 2008/11/18
ジャーナル フリー1. From the urine of rabbits administered (+)-catechin, three neutral substances (F, G and H) were recognized by paper partition chromatography and these substances regarded as intermediary metabolites of catechin.
2. Amoung these three substances, G and H were isolated in crystalline states and their chemical structure were posturated as δ-(3-hydroxyphenyl)-γ-valerolactone and δ-(3, 4-dihydroxyphenyl)--γ-valerolaetone respectively.
The authors are grateful to M. Shido for his kind helps of elemental analysis.抄録全体を表示PDF形式でダウンロード (272K) -
YUSUZO TSUKADA, YUTAKA NAGATA, SHUSUKE HIRANO, GENKICHIRO TAKAGAKI1958 年 45 巻 12 号 p. 979-984
発行日: 1958/12/25
公開日: 2008/11/18
ジャーナル フリー1. The distribution of C14 from uniformly labelled C14-glucose incubated aerobically with brain slices for 90 minutes was studied using the radio-paperchromatography technique.
2. Effects of various conditions and some metabolic inhibitors which change the glucose metabolism of brain slices upon the pattern of radio-autograph was investigated.
3. The formation of amino acids from plucose was clearly inhibited by metabolic inhibitors of the glycolytic route and the citric acid cycle.
4. Glutamic acid was thought to function possibly as a connecting gate between glucose metabolism and amino acids in brain tissue.抄録全体を表示PDF形式でダウンロード (340K) -
AKITANE MORI1958 年 45 巻 12 号 p. 985-990
発行日: 1958/12/25
公開日: 2008/11/18
ジャーナル フリーIn the present experiment concerning the action of both γ-aminobutyric acid and substances possessing similar chemical structure upon the hexokinase activity of the brain and the heart muscle, the following conclusions were drawn.
1. γ-aminobutyric acid acceleates the brain-hexokinase activity slightly while it increases the hexokinase activity of the heart muscle quite markedly.
2. γ-Amino-α-hydroxybutyric acid as well as γ-amino-β-hydroxybutyrie acid do not accelerate the hexokinase activity.
3. N-Acetyl-, γ-aminohutyric acid inhibits the hexokinase activity of the brain and the heart muscle quite strikingly.
4. β-Alanine does not accelerate the hexokinase activity in the brain and the heart muscle, but δ-amino-n-valeric acid promotes the hexokinase activity in the heart muscle.
5. n-Butylamine possesses the power to increase the hexokinase activity.
6. It is assumed that the accelerating power of γ-aminobutyric acid on the hexokinase activity is due to the NH2-CH2-CH2-CH2-group.
I gratefully acknowledge the valuable guidance of Prof. D. Jinnai in preparation of this manuscript.抄録全体を表示PDF形式でダウンロード (363K) -
ARASUKE NISHI1958 年 45 巻 12 号 p. 991-1004
発行日: 1958/12/25
公開日: 2008/11/18
ジャーナル フリー1. Using glycylglycine dipeptidase isolated from baker's yeast, kinetic studies were performed with special reference to the effect of various bivalent ions acting as activators or inhibitors for the enzyme reaction.
2. Among the various metal ions studied, only Co++ was found to activate the enzyme reaction over a wide range of concentration.
3. The activity of the enzyme (in the presence of Co++ as the activator) is strongly inhibited by Cd++, Cu++, Mn++, and Zn++. The inhibitory action of these ions was found to be competitive with the activating effect of Co++. Under the experimental conditions studied, Ni++, Fe++, and Ba++ had no effect on the reaction rate.
4. A scheme of reaction of glycylglycine dipeptidase was proposed, which was shown to be in harmony with the experimental results obtained. It was concluded that a bridge formation by virtue of Co++ ion between enzyme and substrate is an essential step in the mechanism of the enzyme reaction.
5. The enzyme was found to be strongly inhibited by SH-inhibitor such as p-chlorornercuribenzoate and monoiodoacetate.
The author wishes to thank Prof. A. Takamiya and Prof. H. Tamia for their valuable suggestions and encouragement in this work.抄録全体を表示PDF形式でダウンロード (638K) -
TOYOYASU HIROKAWA, SHIGETOH MIYACHI, HIROSHI TAMIYA1958 年 45 巻 12 号 p. 1005-1010
発行日: 1958/12/25
公開日: 2008/11/18
ジャーナル フリー1. Using intact cells of Chlorella, which had been pretreated with sodium azide (10-4M to inhibit the activity of catalase, investigations were made-by the “pre-illumination” experiments using C14O2 according to Calvin and Benson-on the effect of H2O2, upon the photogenic capacity (reduc-ing agent) for CO2-fixation.
2. It was demonstrated that the level of the C14O2-fixing capacity was markedly lowered in the presence of H2O2), indicating that the reducing agent was oxidized by H2O2. Based on the fact that the same oxidizing effect has been observed with oxygen and quinone, it was inferred that H2O2 shares with oxygen and quinone the property of acting as a Hi11 oxidant. The reactivity of these oxidants towards the reducing agent in question was found to be in the order:
quinone<H2O2<O2.
Along the line of reasoning developed in a previous work it was discussed that (i) H2O2, is formed in green cells as a result of reaction of O2, with the reducing agent; that (ii) in normal cells the H2O2 thus formed is immediately decomposed by catalase, and that (iii) the inhibitory effect of cyanide upon photosynthesis is due to the inhibition of catalase leading to the accumulation of H2O2 which consumes the reducing agent that plays the key role in reducing phosphoglyceric acid to triosephosphate in the mechanism of photosynthesis.
This study was aided by grants from the Nishina Memorial Foundation, the Rockefeller Foundation, and the Ministry of Education. To these bodies we extend our grateful thanks.抄録全体を表示PDF形式でダウンロード (330K) -
II. DISTRIBUTION AND PROPERTIES OF D-ARGINASE (D-ARGININE-SPLITTING ARGINASE)YUKIAKI NADAI1958 年 45 巻 12 号 p. 1011-1020
発行日: 1958/12/25
公開日: 2008/11/18
ジャーナル フリーThe distribution in animal organs and some properties of D-arginase (D-arginine-splitting arginase) have been investigated.
1. The splitting of D-arginine can be quantitatively detected at high enzyme concentrations. The activity is markedly variable depending upon the kind of tissues: it is strong in the liver of man, rabbit, and mouse, weak in tissues of dog and toad, and only in trace in tissues of hog, cow, and rat.
2. D-Arginase is so unstable, in contrast to L-arginase, that the activity is lost by heating at 55°, by autolysis at 37°, and even by standing at 0°.
3. D-Arginase has a pH optimum of 9.5. It is activated by manganous, cobaltous, and ferrrus ions and inhibited by lysine, ornithine, heavy-metal ions, and fluorides.
4. Mouse liver extract has been found to split D-arginine into urea and D-ornithine, the latter compound being isolated from the digest mixture as its dibenzoyl derivative and identified.
The author wishes to express his hearty thanks to Prof. S. Utzino for invaluable suggestions in the course of the present experiment.抄録全体を表示PDF形式でダウンロード (553K) -
I. THE REQUIREMENT OF PURINES AS GROWTH FACTORS AND THE EFFECT OF ANTIMETABOLITES ON THE GROWTHTOMOHARU OKUDA, MIKIHISA SAWA1958 年 45 巻 12 号 p. 1021-1027
発行日: 1958/12/25
公開日: 2008/11/18
ジャーナル フリーThe purine-requirement and effects of purine antimetobolites in Leuconosloc mesenteroides P-60 were studied. This strain required one of the following preformed exogenous purines for growth: guanine, xanthine, hypoxanthine and isoguanine, but not adenine. 8-Azaguanine and 8-azaxanthine showed competitive growth inhibiting effects.
The authors with to acknowledge the helpful advices and suggestions of Dr. Y. Miura and Dr. K. Kitahara of the University of Tokyo.抄録全体を表示PDF形式でダウンロード (308K) -
X. THE EFFECTS OF VARIOUS AGENTS ON CRYSTALLINE FUMARASEMINORU INAGAKI, TAKAHIRO MASUDA, KAZUO OKUNUKI1958 年 45 巻 12 号 p. 1029-1036
発行日: 1958/12/25
公開日: 2008/11/18
ジャーナル フリーThe relationship between the inactivation of crystalline fumarase and concentration of bacterial proteinase was investigated. Increase in concentration of the proteinase accelerated the rate of the inactivation of fumarase. At a 1:1 molar ratio, fumarase was about 50 per cent inactivated in one hour. Fumarase was not inactivated by dilution.
Nucleotides and nucleic acid did not protect fumarase from inactivation by the proteinase.
Like other enzymes, fumarase was inactivated by urea. This inactivation was probably caused by a modification of the structure of the fumarase protein.抄録全体を表示PDF形式でダウンロード (400K) -
MASAMITI TATIBANA, MAKOTO NAKAO, HARUHISA YOSHIKAWA1958 年 45 巻 12 号 p. 1037-1038
発行日: 1958/12/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (143K)
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