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47 巻, 5 号
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AKIRA TSUKAMOTO1960 年 47 巻 5 号 p. 559-572
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリー1. It was found that the yeast cells oxidize o-phthalate coupled with aerobic oxidation of various substances (such as formate, acetate, lactate, ethanol, formaldehyde, vanillin, etc.) whose dehydrogenation is known to be catalyzed by DPN-linked dehydrogenases contained in yeast cells. Per mole of phthalate oxidized, 6.7 moles of oxygen were consumed and 6.3 moles of carbon dioxide were evolved.
2. It was revealed that a similar phenomenon could be brought about also by a cell-free preparation consisting of DPN, a labile enzymatic fraction, and a relatively stable fraction consisting various DPN-linked dehydrogenases as well as catalase. In this case, however, no liberation of CO2 occurred and only l mole of O2 was absorbed per mole of phthalate consumed. Nitro-aniline test showed that some phenolic compound(s) was formed as the product of phthalate oxidation.
3. Using the cell-free preparation it was demonstrated that the coupled oxidation of phthalate is inhibited by the addition in excess of catalase. The inhibition of the same process was also brought about by the addition of methanol, which is known to cause, in the presence of catalase and hydrogen peroxide, the trapping of hydrogen peroxide. It was also shown that in the presence of hydrogen peroxide, phthalate is oxidized without being coupled with the oxidation of DPN-linked substrates.
4. Based on these observation it was inferred that in the coupled oxida-tion of phthalate, there occurs an intermediate formation of H2O2, which may function-in the presence of some peroxidase-like enzyme-as the virtual oxidant for phthalate. The state of affairs may reasonably be pictured as has been mentioned in p. 559, where AH2 stands for the DPN-linked “co-substrate” such as formate or alcohol.
5. The reaction (both in vivo and in vitro) is strongly inhibited by cyanide and azide, but not by α, α'-dipyridyl and diethyldithiocarbamate. It is also inhibited by SH-inhibitors (e.g. monoiodoacetate and Hg++-ion) and by some aromatic monocarboxylic acids (e.g. salicylic, benzoic, and phenylacetic acids). Fluoride inhibits the in vivo-reaction.
6. Besides phthalate the following substances were found to be oxidized in a similar manner, although at considerably lower rates than phthalate: 4-and 3-hydroxyphthalates, phenylglycine-o-carboxylate and p-hydroxybenzoate.
The author wishes to thank Profs. Dr. H. Tamiya and Dr. A. Takamiy a of Tokyo University for their kind advice and interest in this work. The author is also indebted to Dr. M. Shiota of Ochanomizu University and Dr. S. Natori of Tokyo University for their helpful suggestions in the preparatory work of hydroxy derivatives of phthalate.
This work was partly aided by a Grant from the Scientific Research Fund of the Ministry of Education.抄録全体を表示PDF形式でダウンロード (909K) -
KUNIHIKO SAITO1960 年 47 巻 5 号 p. 573-580
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリー1. Sphingosine reacts with fluorescein-sodium on filter paper and gives specific orange red colour spot. The detection limit of the reaction is about 1.5 μg.
2. The use of the reaction for quantitative analysis, densitometrically performed, can be employed within the range of 4-16 μg.
The author wishes to thank Prof. S. Akashi, for his interest and encouragements during the present work.抄録全体を表示PDF形式でダウンロード (413K) -
VI. CONVERSION OF SERINE TO GLYCINE IN THE SILKWORM LARVATOSHIFUMI FUKUDA1960 年 47 巻 5 号 p. 581-583
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリー1. The current work was carried out, using L-serine all labeled with C14, to examine whether serine is utilized for the synthesis of glycine in the silk by Bombyx mori.
2. The isotope (C14) of the L-serine all labeled with C14 given to the silkworms appeared in both the 1 C and the 2 C position of the glycine isolated from the fibroin produced by these silkworms.
3. These facts seem to suggest that the action which produces glycine from serine is of importance in the synthesis of the glycine of the silk in addition to the glycine formation by transamination from glyoxylic acid in the silkworm.抄録全体を表示PDF形式でダウンロード (163K) -
II. SOME PROPERTIES OF THE OXIDATIVE PHOSPHORYLATION SYSTEM OF MITOCHONDRIA FROM ASPERGILLUS ORYZAEKOZO IWASA1960 年 47 巻 5 号 p. 584-591
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリーSome properties of mitochondria from Aspergillus oryzae were studied. Only succinate dehydrogenase activity remained. Other dehydrogenases such as isocitric, lactic, pyruvic, α-ketoglutaric, β-hydroxybutyric, and the reduced DPN-oxidizing enzyme system were lost during preparation of the mito-chondria.
Cytochrome c and serum albumin prevented loss of activity to a certain extent. A nucleotide complex prepared from the fungal mats prevented aging of the mitochondria. The active materials in this preparation are nucleotides commonly found in the cells. Stimulation of phosphorylation was also observed, but the action mechanism is still obscure.
The author wishes to express his thanks to Prof. Okunuki for his valuable guidance. Thanks are due to his colleagues Mr. T. Higashiyama of the same laboratory for their help and discussion, and Dr. Inagaki of the same laboratory for kindly supplying glutamic dehydrogenase.抄録全体を表示PDF形式でダウンロード (435K) -
IV. MODE OF EXISTENCE OF NATIVE AND MODIFIED CYTOCHROME CMITSUHIRO NOZAKI1960 年 47 巻 5 号 p. 592-599
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリー1. Sedimentation constants, diffusion coefficients and partial specific volumes of native ferro-, ferri-cytochrome c, and of ferri-cytochrome c modified by trichloroacetic acid were measured. Sedimentation constants at 20° in water (S20, w) are 1.87, 1.91 and 2.50 Svedberg units for native ferro-, ferri- and modified ferri-cytochrome c, respectively. Diffusion coef-ficients at 20° in water (D20, w) are 12.9×10-7, 13.2×10-7 and 8.5×10-7cm.2/ sec. for native ferro-, ferri- and modified ferri-cytochrome c, respectively. The partial specific volumes of these different kinds of cytochrome c's are 0.70.
2. From these values, the molecular weight of the native cytochrome c is calculated to be 12, 000 and that of modified ferri-cytochrome c to be 24, 000. This indicates that the molecules of modified cytochrome c exist as dimers of molecules similar to those of native cytochrome c.
The author would like to express his thanks to Prof. K. Okunuki and Dr. T. Horio for their valuable guidance, and is grateful to Prof. T. Isemura and Prof. K. Fukai of this university for helpful advice on the physicochemical measurements.抄録全体を表示PDF形式でダウンロード (536K) -
II. ADAPTIVE FORMATION OF CYANIDE RESISTANT RESPIRATORY SYSTEM IN RESTING CELLSSHÔJI MIZUSHIMA, KEI ARIMA1960 年 47 巻 5 号 p. 600-607
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリー1. It was demonstrated that the respiratory system of a strain of Achromobacler could adaptively become cyanide resistant without bacterial growth.
2. During the adaptation, the amount of cytochrome a2 in resting cells, remarkably increased as in the case of the adaptation in growing bacteria.
3. Cyanide, energy source and amino acids were required for the adaptation. The optimal concentration of cyanide in the medium was 1_??_2×10-3M.
4. Streptomycin (10-3M) inhibited the bacterial growth but did not inhibit the adaptation to cyanide.
5. Chloramphenicol (10-5M) only inhibited the adaptation to cyanide but did not inhibited the bacterial growth in bouillon medium.
6. The mechanism of the adaptation was discussed especially with regard to the mode of action of chloramphenicol.
The authors wish to express their sincere appreciation to Dr. K. Sakaguchi for his kind guidance throughout the course of this study.抄録全体を表示PDF形式でダウンロード (444K) -
IV. ACTION OF RIBONUCLEASE T1 ON DEAMINO-RIBONUCLEIC ACIDKIMIKO SATO-ASANO, YOSHIKO FUJII1960 年 47 巻 5 号 p. 608-615
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリー1. It was proved that RNase T1 could digest the deamino-yeast RNA and hydrolyze the secondary phosphate ester bonds of inosine-3' phosphate and xanthosine-3' phosphate with the intermediary formation of respective nu-cleoside-2', 3' cyclic phosphate during the digestion.
2. It seems that for the action of RNase T1 hydroxyl (or keto) group attached to the carbon atom 6 of purine ring is necessary.
3. Two new compounds, inosine-2', 3' cyclic phosphate and xanthosine-2', 3' cyclic phosphate, were obtained from the digestion mixture of deamino-yeast RNA by RNase T1.
The authors wish to thank Prof. F. Egami for his kind guidance and encourage-ment. They also acknowledge the gift of “Takadiastase Sankyo” by Sankyo Parma-ceutical Co. Ltd.抄録全体を表示PDF形式でダウンロード (404K) -
II. THE STATE OF ELECTRON CARRIERS AND THE ADENOSINE TRIPHOSPHATE-PHOSPHATE EXCHANGE REACTIONKOZO IWASA1960 年 47 巻 5 号 p. 616-624
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリー1. Using rat liver mitochondria the relation of the ATP-iP exchange reaction to oxidative phosphorylation was studied.
2. Extreme reduction of the carriers inhibited the exchange more than oxidation of the carriers.
3. When the carriers were slightly reduced by a low concentration of cyanide or a nitrogen gas phase, the exchange reaction increased probably owing to formation of phosphorylated intermediates.
4. Alternation of the gas phase from nitrogen (preincubation) to air (incubation) enhanced the exchange reaction.
5. As with the DNP-activated ATP-ase and oxidative phosphorylation reactions reported by Slater et al. (2, 3, 4), there were three pH optima on the exchange reaction.
6. The height of the optima peaks varied with the oxidation-reduction state of the carriers at each oxidative phosphorylation step.
The author wishes to express his gratitude to Prof. K. Okunuki for his guidance. He would like to thank his colleague Mr. Higashiyama of the same laboratory for helpful discussion during this work.抄録全体を表示PDF形式でダウンロード (508K) -
TAMITARO MIZUNOYA1960 年 47 巻 5 号 p. 625-629
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリーA detailed expression is given to the chemical drag coefficient of the stationary enzyme reaction in nearly equilibrium state. It is clarified in the expression that the coefficient, i.e., the neck factor depends on the amount of enzyme.
The author wishes to express his hearty thanks to Prof. M. Sugita of Hitotsubashi University for his valuable advices in preparation of the manuscript. The author's thanks are also due to Prof. K. Oomori of this laboratory for his encouragement and interest in this work.抄録全体を表示PDF形式でダウンロード (243K) -
KUNIO YAGI, IKUKO NAGATSU1960 年 47 巻 5 号 p. 630-634
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリー1. FMS, the specific inhibitor of flavin enzymes, did not affect the oxygen uptake of freshly prepared mitochondria of rat liver with α-ketoglutarate, succinate, malate, and D-alanine.
No effects of flavins (FAD, FMN, riboflavin and FMS) on the oxygen uptake of the mitochondria were found, even if incubated with these flavins at 4° for three hours.
Against the report of Eggleston and Williamson, FAD did not inhibit the oxygen uptake of liver suspensions.
These results indicate that the flavins in the mitochondria exist in com-pact bound form.
2. On the other hand, by aging the mitochondria at 4° for 5 days, added FAD increased the oxygen uptake and added FMS decreased it.
These results suggest that flavins in the aged mitochondria would exist in such a state as the added flavins could replace them.
3. By aging the mitochondria, apo-protein of flavin enzyme in the mitochondria is partially inactivated. The coexistence of FAD, even FMS, protects the inactivation of the apo-protein during the aging.抄録全体を表示PDF形式でダウンロード (323K) -
II. SEPARATION OF ACID-SOLUBLE PHOSPHORUS COMPOUNDS INCORPORATING P32 BY COLUMN CHROMATOGRAPHY WITH ION EXCHANGE RESINHARUHISA YOSHIKAWA, MAKOTO NAKANO, KANJI MIYAMOTO, MASAMITI TATIBANA1960 年 47 巻 5 号 p. 635-648
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリーAcid-soluble phosphorus compounds of human erythrocytes were analyzed by column chromatography on Dowel 1-chloride and formate with the use of P32 as an indicator. The compound found were uric acid, xanthine, diphosphopyridine nucleotide, adenosine monophosphate, adenosine diphos-phate, adenosine triphosphate, inosine monophosphate, phosphoglyceric acid, 2, 3-diphosphoglyceric acid, glucose 6-phosphate, fructose 6-phosphate, fructose 1, 6-diphosphate, and glucose 1, 6-diphosphate. An adenine nucleotide with two stable and two labile phosphate groups was also found. The concentra-tions of these compounds were determined.
After incubation of erythrocytes with P32 at 37° for 30 minutes the spe-cific activity of stable P and labile P of ADP and ATP, and of 2, 3-diphos-phoglyceric acid was estimated with precision. The highest specific acitivity was found in labile P of the nucleotides, and 2, 3-diphosphoglyceric acid had considerably lower specific activity. A rapid equilibration of specific activity among labile P of ADP and ATP was accomplished by the activity of adenylated kinase in the cell. There was a slight but distinct incorporation of P32 into stable P of ADP and ATP and P of AMP. No P32 incorpration was observed in DPN and TPN fractions.抄録全体を表示PDF形式でダウンロード (995K) -
II. PHYSICO-CHEMICAL PROPERTIES OF “FOURTH FLAVIN COMPOUND”KUNIO YAGI, YOSHITAKA MATSUOKA1960 年 47 巻 5 号 p. 649-653
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリーFFC can be separated from other flavins, FAD, FMN and FR, by paper chromatography.
Paper electrophoresis at different pH showed that FFC has not a second dissociation of phosphoric acid.
The absorption spectrum of FFC is quite similar with that of FMN, and the maxima of it exist at 267, 373 and 445mμ, and molecular coef-ficients are quite similar with those of FMN.
pH-Fluorescence curve of FFC is also similar with that of FMN and the maximum fluorescence energy exists at pH 5.0-7.5, and pKa is 1.97 which is a little smaller than that of FMN, 2.17. The maximum of fluorescence spectrum of FFG is recorded at 530 mμ as same as those of other flavins.抄録全体を表示PDF形式でダウンロード (248K) -
KAZUO SATAKE, TSUNEO OKUYAMA, MOCHIHIKO OHASHI, TOMOTAKA SHINODA1960 年 47 巻 5 号 p. 654-660
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリー1. Primary amine, amino acid, and peptide at a concentration of 0.01 to 0.8μ mols per ml., could be assayed spec trophotometrically by the TNP-lation with TNBS.
2. Under the conditions described, the optical densities of various amino compounds were practically the same as those of the equivalent amount of the corresponding TNP-derivatives, which were 1.27×104 for amino groups of amines and amino acids and 1.05×104 for those of peptides at a final concentration of N, respectively.
3. TNP-lated sample, after the assay, could be recovered from the Spec tropho tome tric mixture and be used for the structural analysis.
We are indebted to Mrs. S. Sasakawa for the information on her unpublished data and to Mr. J. Nitta for the preparation of TNBS. This work was supported in part by the grant of Scientific Research Found of the Ministry of Education for which the authors wish to thank.抄録全体を表示PDF形式でダウンロード (399K) -
III. REGENERATION OF ADENOSINE TRIPHOSPHATE IN LONG-STORED ERYTHROCYTE BY INCUBATION WITH INOSINE AND ADENINEMAKOTO NAKAO, TOSHIKO NAKAO, MASAMITI TATIBANA, HARUHISA YOSHIKAWA1960 年 47 巻 5 号 p. 661-671
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリー1. ATP content of long-stored blood cells was not restored by incubation with inosine alone but a marked increase of ATP level was observed by in-cubation with added adenine together with inosine.
2. By the use of adenine-8-C14, it was demonstrated that added adenine itself was utilized for the synthesis of adenine nucleotides.
3. Regeneration of ATP was accompanied by a shape change of ery-throcytes from spheric to shallow bowl form.
4. The extent of ATP regeneration in aged erythrocytes can be estimated by measuring P32 incorporation into total nucleotide in the cells. By this procedure was studied the influences of adenine and inosine concentrations, preservation period, and metabolic inhibitors. Arsenate, iodoacetate and fluoride inhibited the reaction strongly.
5. Only adenine was effective among various purines and pyrimidines. When various nucleosides and deoxynucleosides were added to the stored blood supplimented with adenine, purine nucleosides and purine deoxynucleo-sides were effective. The nucleosides and possibly the deoxynucleosides are suggested to provide the pentose residue of the adenine nucleotides and the glycolytic intermediates necessary for the supply of phosphate.
6. These findings lead us to a prospect for extending the useful storage life of aged blood cells.
Authors express their thanks to Dr. Miyamoto, the President of Plasma Laborato-ries, Tokyo, and Dr. Tooyam a of the Blood Transfusion Center of the University of Tokyo Hospital for the generous supply of stored blood.抄録全体を表示PDF形式でダウンロード (2387K) -
IV. HISTIDINE PEPTIDES DERIVED FROM HORSE GLOBIN WITH STREPTOMYCES GRISEUS PROTEINASESHIGERU SASAKAWA, KAZUO SATAKE1960 年 47 巻 5 号 p. 672-677
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリー1. Histidine peptides derived from horse globin with Streptomyces griseus proteinase, were separated on a column of Amberlite IRC-50 (pH 5.2) and purified as the α-mono-TNP-derivatives.
2. On the acid-hydrolysis of α-mono-TNP-histidine peptides, α-mono-TNP-histidine from N-terminal and free histidine from the non-N-terminal positions, could be recovered in good yield, respectively.
3. The presences of 4 moles of His-Ala-Ser, 3 moles of His-Ser-Gly, one mole of His-His-Gly, Ala-His-His-Arg, and Ser-His per 35, 000g. of horse globin was elucidated.
The present work was aided in part by the Scientific Research Grant from the Mini-stry of Education.抄録全体を表示PDF形式でダウンロード (352K) -
III. EFFECTS OF METAL IONS ON SOLUBLE AND PARTICULATE COMPONENTS OF NITRITE REDUCTASEAKIRA ASANO1960 年 47 巻 5 号 p. 678-684
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリー1. The particulate component (PF) of nitrite reductase of a halotolerant micrococcus, strain 203, was solubilized by the treatment with snake venom and purified by ammonium sulfate fractionation.
2. Using partially purified and thoroughly dialyzed preparations, it was found that the activity of soluble component (SF) is activated by Cu+ and Cu++ and that of particulate component (PF) is enhanced by Fe+ and Fe+++.
3. Intracellular distribution of CO-binding pigment(s) in this organism was studied.
The author wishes to thank Prof. F. Egami for his kind encouragement and guidance. Thanks are also due to Prof. R. Sato, Institute for Protein Research, Osaka University, for his kind criticism on the manuscript and to Mr. H. Maeno, Gunma University Medical School, for the supply of snake venom.抄録全体を表示PDF形式でダウンロード (438K) -
MICHIO TSUKAMURA1960 年 47 巻 5 号 p. 685-688
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリーThe mechanism of action of viomycin was studied by utilizing isotopic techniques and Mycobacterium avium (Jucho strain).
The incorporation of P32 into the nucleic acid fraction and into the protein fraction were inhibited by viomycin, and the incorporation of S35 into the protein fraction was inhibited by viomycin. It is suggested that viomycin inhibits the biosynthesis of protein and this inhibition may be resulted from damaged functioning of RNA, considering the author's previous observation that viomycin precipiatates RNA.抄録全体を表示PDF形式でダウンロード (218K) -
EMI KUSUNOSE, MASAMICHI KUSUNOSE, YOSHIO KOWA, YUICHI YAMAMURA1960 年 47 巻 5 号 p. 689-693
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (258K) -
MAKOTO NAKAO, TOSHIKO NAKAO, MASAMITI TATIBANA, HARUHISA YOSHIKAWA1960 年 47 巻 5 号 p. 694-695
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (1094K) -
TOKUHIKO HIGASHI, MICHIKO YAGI, HIDEMATSU HIRAI1960 年 47 巻 5 号 p. 696-698
発行日: 1960/05/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (200K)
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