巻号一覧
70 巻, 2 号
選択された号の論文の21件中1～21を表示しています
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XIV. The Electron Donating System in the Reduction of Nitric Oxide and NitrateMachiko MIYATA1971 年 70 巻 2 号 p. 205-213
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーThe electron donating mechanism of the reduction of NO to N2O by the particles of Pseudomonas denitrificans was studies, using phenazine methosulfate reduced with ascorbate tascorbate-PMTS-NO system) and lactate (lactate-NO system) as the electron donor, and the following facts were found.
1. A lag in the NO uptake was observed on the reaction with the lactate-NO system but not with the ascorbate-Plv1S-NO system. The specific activity of the latter system was about three times as high as that of the former.
2. Spectrophotometry under the atmosphere of l0% NO plus 90% argon suggested that b-type cytochrome(s) and particulate eytochrome c-552 participate in the reduction of NO to N2O with both systems.
3. BAL, HOQNO or antimycin A inhibited the nitric oxide reduction in both systems.
4. Addition of nitrate to the lactate-NO system remarkably suppressed the nitric oxide reduction, and nitrate was reduced to nitrite. The ratio of the rate of nitrate reduction to that of nitric oxide reduction was larger than 1.
Based on the observations that nitrate reduction resulted in oxidation of a b-type cytochrome and was not inhibited by BAL, antimycin A or HOQNO, the site of interaction of nitrate was concluded to be the b-type cytochrome. A possible electron transport mechanism in the cellular particles of Ps. denitrificans was discussed from these results.抄録全体を表示PDF形式でダウンロード (626K) -
IV. Regulation of Valine Transport Activity by Valine and CysteineShigeko KANZAKI, Yasuhiro ANRAKU1971 年 70 巻 2 号 p. 215-224
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーThe regulation of valine transport activity of Escherichia coli K12 was studied under several different physiological conditions. Mutants which were sensitive or resistant to .valine were used. The changes induced by valine in the intracellular amino acid pool and their effects upon the common transport system were analysed in detail.
It was found that valine induced dramatic changes in the levels of pool amino acids in valine-sensitive cells, and also derepressed the common transport system for branched chain amino acids of the organism. Valine had the opposite effect to leucine and isoleucine which acted as repressors of this system.
Cvsteine was found to inhibit the growth of valine-sensitive cells of Eschericlaia cdli K12. All the mutants so far examined, which were resistant to valine, were also resistant to cysteine. This amino acid apparently derepressed the constitutive common transport system for branched chain amino acids. Pyruvate, when used as a sole source of carbon, also derepressed the common transport system.
The maintenance and regulation of the amino acid pool is discussed with respect to the dynamic equilibrium between biosynthesis and tranport.抄録全体を表示PDF形式でダウンロード (605K) -
Haruo KAGAWA, Hiroaki TOKIMATSU, Hideo FUKUTOME1971 年 70 巻 2 号 p. 225-234
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーThe ribosomal subunits of Escherichia coli washed well with buffer containing 0.1mM magnesium ion fail to associate with the partner subunits even at high (10mM or more) magnesium ion concentrations when examined at low temperature. However, they recovered the ability to associate when they were preincubated at 37°C with magnesium and univalent (ammonium) ions. Preincubation of the 30S subunit only was sufficient for the recovery but that of the 50S subunit only was ineffective. Spermine, poly A, poly U or poly U and tRNA accelerated the recovery. Also, a factor contained in the post-ribosomal fraction was effective. From the study of magnesium dependence of poly U-directed polyphenylalanine synthesis, the 70S ribo-somes recovered with poly A or the supernatant factor were found to be different from those recovered with spermine or with uni- and divalent cations.抄録全体を表示PDF形式でダウンロード (640K) -
Hajime YAMASAKI, Kazumi YAMASAKI1971 年 70 巻 2 号 p. 235-241
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (1048K) -
Reiji YAMBE, Tosihiko HUKUI, Syozo TUBOI1971 年 70 巻 2 号 p. 243-247
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーSuccinate semialdehyde dehydrogenase [EC 1. 2. 1. 16] was purified from Rhodopseudomonas spheroides about 250 fold. The succinate semialdehyde dehydrogenase in R. spheroides was active both with NADP+ and with NAD+. The Km value for NAD+ (O.8 mM) was much larger than that for NADP+ (0.06mM), while the Km value for succinate semialdehyde measured with NAD+ was identical with that measured with NADP+. The Vmax obtained with NAD+ was considerably larger than that obtained with NADP+, and the pH optima with these electron acceptors were very close to each other.抄録全体を表示PDF形式でダウンロード (301K) -
Kohei UMEZU, Tadahiro AMAYA, Akio YOSHIMOTO, Kenkichi TOMITA1971 年 70 巻 2 号 p. 249-262
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーOrotidine-5'-phosphate (O5P) pyrophosphorylase [EC 2. 4. 2. 10] and O5P decarboxylase [EC 4. 1. 1. 23] were separately purified from bakers' yeast about 1, 800- and 6, 000-fold, respectively. O5P pyrophosphorylase activity was resolved into major and minor components on DEAE-cellulose. Molecular weights were estimated to be 39, 000 for the main- and 32, 000 for the minor-component of O5P pyrophosphorylase, and 51, 000 for O5P decarboxylase from their elution positions on Sephadex G-100 gel filtration.
Purified O5P pyrophosphorylase had the pH optimum at 8.5, generally stable at pH above 7.5, and catalyzed both the O5P formation (forward reaction) and the O5P pyrophosphorolysis (reverse reaction) at about the same initial rate. The Km values were estimated to be 33 μm for orotate, 62 μm for 5-phosphoribosylpyrophosphate, 8.3 μm for 05P and 220 μm for PPi. Its enzymatic activity or its stability was not affected by the presence of sulfhydryl compounds or sulfhydryl reagents.
On the other hand, purified O5P decarboxylase had the pH optimum at 5.5, generally stable at pH below 7.5, and unstable in a frozen state. The enzyme was not fully active in the absence of sulfhydryl compounds, and fully activated by cysteine, reduced glutathione (GSH), dithiothreitol (DTT) or μ-mercaptoethanol. For the long storage at 4°C, however, μ-mercaptoethanol was most effective in protect-ing the enzyme from inactivation. This enzyme was also sensitive to sulhydryl reagents like p-chloromercuribenzoate and 5, 5'-dithiobis (2-nitrobenzoic acid).
The forward reaction of O5P pyrophosphorylase was markedly inhibited by 05P, the product, but not inhibited by most of other nucleotides. In contrast, O5P decarboxylase was moderately inhibited by GMP, GDP and CMP, but not inhibited by other nucleotides including uridine nucleotides. The Km value for orotate was estimated to be 5μM, and the Ki values were calculated to be 1.2mM for GMP and 0.28mM for CMP.抄録全体を表示PDF形式でダウンロード (910K) -
Teruhiko BEPPU, Kei ARIMA1971 年 70 巻 2 号 p. 263-271
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーDegradation of DNA in the growing Esclzeriehia colt induced by relatively high dose of colicin E2, seemed to proceed in two steps. The first step, probably endonucleolytic one, was accompanied by rapid decrease in amounts of DNA-membrane complex and morphological change of bacterial nuclear body. Prolonged inhibition of protein synthesis before the addition of colicin caused suppression of the degradation probably due to suppression of the second exonucleolytic reaction. On the other hand, thymine starvation stimulated the degradation remarkably. Specific regions of the bacterial chromosome, i.e., regions near the initiation point and near the replicating point, did not show selective sensitivity for the colicin action. Cells which adsorbed colicin E2 could divide at least once even when their chromosomes were actively degraded. Several metabolic inhibitors were described to inhibit the DNA-degradation.抄録全体を表示PDF形式でダウンロード (1250K) -
Wayne E. CRISS1971 年 70 巻 2 号 p. 273-278
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーRat liver mitochondrial adenylate kinease [ATP: AMP phosphotransferase, EC 2. 7. 4. 3] was observed to “release from” and “rebind to” mitochondria. The following conditions influence this observed adenylate kinase flux:
1. Phosphate caused a more rapid release and an alkaline pH allowed a greater extent of adenylate kinase release from mitochondria.
2. None of the nucleotides, which were tested, including ATP, ADP, and AMP, prevented the phosphate induced release.
3. All divalent cations, which were tested, completely inhibited the phosphate induced release.
4. Uncouplers of oxidative phosphorylation partially prevented the phosphate induced release. Atractyloside had no effect.
5. The swelling of the mitochondria paralleled release of adenylate kinase from the mitochondria.
6. Released adenylate kinase activity will “rebind” to mitochondria which are partially swollen, but will not “rebind” to completely swollen mitochondria.抄録全体を表示PDF形式でダウンロード (360K) -
VI. Co-operative Transition of ATPase Activity during the Initial PhaseShinpei YAMADA, Taibo YAMAMOTO, Tohru KANAZAWA, Yuji TONOMURA1971 年 70 巻 2 号 p. 279-291
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーThe Departinent of Biology, Faculty of Science, Osaka University, Toyonaka, Osaka A remarkable decrease in the ratio of the rate of Pi-liberation (v) to the amount of phosphorylated intermediate ([EP]) was observed during the initial phase of the Ca2+-dependent ATPase [ATP phosphohydrolase: EC 3.6.1.3] reaction of SR. The effects of ATP, pH, divalent cations, Triton X-100 and NEM on the transition in v/[EP] were investigated, and the following results were obtained.
1. A typical transition in v/[EP] during the initial phase of the reaction was observed at alkaline pH as well as at neutral pH. At alkaline pH the amount of Pi liberated from ATP during the transition phase was far less than that of the phosphorylation site of SR. The value of v/[EP] in the steady state was unchanged by the presence of oxalate (0 to 5 mm). The extent of the decrease in v/[EP] was not affected by changing the concentration of Mg2+.
2. The extent of decrease in v/[EP] after starting the reaction by adding Ca2+ was less than that after starting the reaction by adding ATP.
3. When SR was pretreated with Triton X-100 (20 μl/mg SR protein), the value of v/[EP] in the steady state was greatly increased and its transition disappeared almost completely. The transition also disappeared on treatment of SR with NEM.
4. The transition in the value of v/[EP] occurred completely even in the pres-ence of ATP, at a much lower concentration than that of the phosphorylation site. The transition in v/[EP] after adding 0.05 μ M ATP was rather slow, and 45 sec-1 and 0.45 sec-1 were obtained as the initial and steady state values, respectively, of v/[EP] at 15°C.
From these results, we concluded that the transition in v/[EP] results from a cooperative conformational change in the vesicular structure of SR induced by ATP.抄録全体を表示PDF形式でダウンロード (2747K) -
XXIV. Fluorescence of 3-Benzyl-4-methyl-7-diethylaminocoumarin in the Presence of α-Chymotrypsin and Its DerivativesYoshihisa NISHIMURA, Osamu TAKENAKA, Kazuo SHIBATA1971 年 70 巻 2 号 p. 293-299
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーThe quantum yield and the maximum wavenumber of the fluorescence of a coumarin dye, 3-benzyl-4-methyl-7-diethylaminocoumarin (BMDC) were measured in water in the presence and absence of α-chymotrypsin [EC 3. 4. 4. 5] or its inactive forms such as chymotrypsinogen, alkali-denatured α-chymotrypsin, N-tosylphenylalanyl chloromethyl ketone (TPCK)-modified chymotrypsin and diisopropylfluorophosphate (DIP)-modified chymotrypsin. A great increase of the quantum yield of fluorescence and a blue shift of the fluorescence band were found by addition of α-chymotrypsin while addition of chymotrypsinogen, TPCK- and DIP-chymotrypsin caused only small changes of the fluorescence band. The changes caused by α-chymotrypsin were suppressed by co-existence of β-phenylpropionic acid, a competitive inhibitor of the enzyme. The fluorescence of 4-methyl-7-diethylaminocoumarin with no benzyl group was less affected by α-chymotrypsin. The fluorescence band of BMDC was enhanced and shifted similarly when the medium was changed from water to alcohols or ethanol-water mixtures. It was deduced from these results that BMDC is adsorbed on the specificity-determining site of α-chymotrypsin which is to be of aromatic nature and that the site is formed on activation of chymotrypsinogen.抄録全体を表示PDF形式でダウンロード (473K) -
pH-Profiles of Kinetic ParametersMasachika IRIE, Shunji HOSOKAWA1971 年 70 巻 2 号 p. 301-309
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリー(1) The rates of cleavage of dinucleoside phosphates (XpY) and of hydrolysis of nucleoside cyclic phosphates by RNases from bovine seminal vesicles (RNase Vs1 and Vs2) [EC 2. 7. 7. 16] were measured at pH 7.0 to obtain kinetic parameters, Km and Vmax For both RNases, an accelerating effect of the base of the 3'-OH end nucleoside was observed in the following order U<C_??_G<A.
(3) Kinetic parameters, Km and Vmax, of RNase Vs1 and Vs2 were obtained using cyclic UMP and cyclic CMP as substrates at various pH's. From the pKm- and log Vmax-pH profiles, pKa's of two dissociable groups in the free enzyme were estimated to be 6.0 and 4.0 for RNase Vs1 and 6.0-6.25 and 4.25 for RNase Vs2. Based on these values, the possibility of the presence of histidine or carboxyl residues in the active site of the enzymes was discussed.
(4) From the comparison of pKm-pH profiles of cyclic UMP and cyclic CMP for both enzymes, it was concluded that the protonation of substrates makes the binding with the enzymes unfavorable.
(5) RNase Vs1 and RNase Vs2 were found to be inhibited competitively by various kinds of nucleotides. The inhibitory effect of the nucleotides having a phosphate group at the 2'-position of ribose increased in the order of G<U<C for both en-zymes. The inhibitory effect of cytidine nucleotides increased in the order of 5'<3'<2' for both enzymes.
(6) The difference spectrum caused by the binding of 2'-CMP and RNase Vs1 indicated that one mole of 2'-CMP binds with one mole of RNase Vs1 at pH 5.0.抄録全体を表示PDF形式でダウンロード (526K) -
Tatsuo NAKAYAMA, Koiti TITANI, Kozo NARITA1971 年 70 巻 2 号 p. 311-326
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーBonito cytochrome c was digested with trypsin, and the resulting peptides were fractionated by chromatography on a Dowex 1 column with volatile buffers. Hetero-geneous peptide fractions were further resolved by Dowex 50 chromatography followed by paper electrophoresis or paper chromatography. The amino acid sequences of the tryptic peptides were determined mainly by the improved Edman method. Information on the arrangement of the tryptic peptides was obtained from the bridge peptides derived from the protein by treating with cyanogen bromide followed by chymotryptic digestion. The complete amino acid sequence of 103 residues was deduced. The number of total amino acid residues was less than that of other vertebrate cytochromes c (104 residues) with the exception of that of tuna cytochrome c. Only difference in the amino acid sequence between the tuna protein and the bonito protein was recognized at the 61st position.抄録全体を表示PDF形式でダウンロード (791K) -
Yoshihisa NAKANO, Shozaburo KITAOKA1971 年 70 巻 2 号 p. 327-334
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーThe properties of L-aspartate α-decarboxylase [EC 4. 1. 1. 11] in a crude extract and in a partially purified preparation from E. coli B were studied.
1. Adaptation of the bacteria to substrate by growth in synthetic medium containing 1.5% L-aspartic acid and 0.5% L-glutamic acid before culture in the same medium greatly increased enzyme production. Enzymatic activity increased with bacterial growth to a maximum in the later stage of the logarithmic phase and decreased promptly toward the beginning of the stationary phase, indicating that the enzyme was formed to supply β-alanine required during bacterial growth for biosynthesis of pantothenic acid. Formation of β-alanine from uracil by adapted bacteria was rather slight.
2. The optimum pH of this enzyme was 5.5 and the optimum temperature was rather broad, being 37-47°C. With a partially purified enzyme preparation the Michaelis constant was calculated to be 1, 5×10-3M.
3. Pyridoxal 5'-phosphate, α-keto acids and Co2+ and Mg2+ ions activated the enzyme, and their effects were additive. A combination of pyridoxal 5'-phosphate, α-keto-glutaric acid and Co2+ enhanced the enzymatic activity about 13-fold. Cyanide, fluoride, Ag+, Hg2+, semicarhazide, thiourea and isonicotinic acid hydrazide inhibited the decarboxylase.抄録全体を表示PDF形式でダウンロード (462K) -
I. Gas Chromatographic Analysis of Common Constituents of GlycolipidsSusumu ANDO, Tamio YAMAKAWA1971 年 70 巻 2 号 p. 335-340
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーA method is described to determine gaschromatographically the individual component of glycosphingolipid after methanolysis and trifluoroacetylation. By using a mixed coated column of SE-30 and XE-60 and under suitable temperature-programmed conditions, neutral hexoses, hexosamines, neuraminic acid, and sphingosine bases from glycolipids were analyzed in one run with considerable accuracy.抄録全体を表示PDF形式でダウンロード (384K) -
Noriko ANDO, Susumu ANDO, Tamio YAMAKAWA1971 年 70 巻 2 号 p. 341-348
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーThin-layer chromatography of the cephalin fraction, treated thoroughly with acetone, revealed an unknown spot just above the phosphatidyl ethanolamine. It was isolated and examined by IR, mass, NMR spectroscopies and chemical synthesis in comparison with some reference compounds. Finally it was shown as follows.
(1) The cephalin artifact was a mixture of N-isopropylidene- and N-(1, 1-dimethyl-3-oxo-butyl)-derivatives of phosphatidyl-ethanolamine.
(2) The latter compound was the major component in the artifact fraction.
(3) The N-isopropylidene form was derived from phosphatidyl-ethanolamine in an acetone solution at 42°C under anhydrous conditions.
(4) The N-(l, 1-dimethyl-3-oxo-butyl)-derivative is thought to be produced by condensation of two acetone molecules to phosphatidyl-ethanolamine preferably at low temperature.抄録全体を表示PDF形式でダウンロード (461K) -
Tateo YAMANAKA, Saburo TAKENAMI, Noriko AKIYAMA, Kazuo OKUNUKI1971 年 70 巻 2 号 p. 349-358
発行日: 1971/08/25
公開日: 2008/11/18
ジャーナル フリーTwo kinds of C-type cytochromes, cytochrome c-550 and cytochrome c-551, derived from the facultative chemoautotroph, Thiobacillus novellus, were highly purified. Cytochrome c-550 possessed absorption peaks at 410 my in the oxidized form, and at 414.5, 520 and 550 mμ in the reduced form. The isoelectric point of the cytochrome was at pH 7.5, and the midpoint redox potential+0.276V. Amino acid analysis showed that cytochrome c-550 was composed of 118 amino acid residues and its molecular weight was 13, 270. The cytochrome reacted fairly rapidly with cow cytochrome oxidase [EC 1.9.3.1], very rapidly with yeast cytochrome c peroxidase [EC 1.11.1.5], and poorly with Pseudomonas aeruginosa nitrite reductase [EC 1.9.3.2].
Cytochrome c-551 had absorption maxima at 410.5 mμ in the oxidized form, and at 416, 522 and 551 mμ in the reduced form. The isoelectric point and midpoint redox potential of cytochrome c-551 were at pH 5.2 and +0.260V, respectively. The cytochrome did not react with either of the animal cytochrome oxidase, the yeast peroxidase and the bacterial nitrite reductase.
Sulphite: cytochrome c oxidoreductase partially purified from the organism reduced very rapidly both the C-type cytochromes with sulphite as the electron donor. The enzyme reacted rapidly also with cow and yeast cytochromes c, but did not react with cytochrome c (551, Pseudomonas aeruginosa), cytochrome c (552, Pseudomonas stutzeri) or cytochrome c (553, Endarachne binghamiae). The evolutionary position of T. novellus was discussed on the basis of the enzymatic properties of cytochrome c-550 and of the specificity of sulphite: cytochrome c oxidordectase for cytochrome c used as the electron acceptor.抄録全体を表示PDF形式でダウンロード (708K) -
Tahei NEGI, Tatsuya SAMEJIMA, Masachika IRIE1971 年 70 巻 2 号 p. 359-363
発行日: 1971/08/25
公開日: 2008/11/18
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Kazuhiko ADACHI, Tsuneo OKUYAMA1971 年 70 巻 2 号 p. 365-366
発行日: 1971/08/25
公開日: 2008/11/18
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Yuichi KOMAI, Shigeko MATSUKAWA, Mei SATAKE1971 年 70 巻 2 号 p. 367-369
発行日: 1971/08/25
公開日: 2008/11/18
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Yoshiaki KOMIYA, Michihiro KASAHARA1971 年 70 巻 2 号 p. 371-374
発行日: 1971/08/25
公開日: 2008/11/18
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1971 年 70 巻 2 号 p. e1-e3
発行日: 1971年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (91K)
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