巻号一覧
65 巻, 4 号
選択された号の論文の23件中1～23を表示しています
- |<
- <
- 1
- >
- >|
-
TAKAHIRO HARADA, HIROMICHI OKUDA, SETSURO FUJII1969 年 65 巻 4 号 p. 497-501
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーA crude preparation of rat liver acyl-CoA synthetase [EC 6. 2. 1. 3] was stabilized by EDTA. It was found that acyl-CoA synthetase increased 2 to 3 fold in the livers of starved and diabetic rats, and of rats fed on a high fat diet. Among various inter-mediates of the tricarboxylic acid cycle, citrate and isocitrate inhibited acyl-CoA synthe-tase. A physiological significance of the citrate inhibition was discussed.抄録全体を表示PDF形式でダウンロード (338K) -
AKIRA HAYASHI, TOSHIKO MATSUBARA1969 年 65 巻 4 号 p. 503-511
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーThe glycolipids in oyster mantle were extracted with chloroform: methanol (1:3) and purified by the solvent fractionation method, and silicic acid column chromato-graphy. The lipids eluted with chloroform: methanol (2:3) and (1:4) were mucolipids of the globoside type and consisted of fatty acid, sphingosine, and sugar moiety. Sugar moiety was composed of glucose, galactose, fucose, and O-alkyl-fucose as neutral sugar, and glucosarnine as amino sugar. Besides these sugars, an amino sugar phosphate was found as a component of the lipid. Fatty acids consisted of hexadecanoic, heptadecanoic, and octadecanoic acids as major components. In addition to the usual C18-sphingosine, a C16-like sphingosine and an unidentified base were found as long chain bases.抄録全体を表示PDF形式でダウンロード (510K) -
II. Inhibition of Protein Synthesis in the Microsome-pH 5 Fraction System of Thymus Cells Following the Administration of TriamcinoloneTETSUO UETE1969 年 65 巻 4 号 p. 513-521
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリー1. A study was carried out to clarify the nature of the impaired capacity of the thymus microsome-pH 5 fraction system to incorporate amino acids into proteins 2 to 3 hr after the administration of triamcinolone to rats.
2. The synthesis of amino-acyl-RNA in the pH 5 fraction [amino-acyl-RNA synthe-tases, EC 6. 1. 1 group] of 105, 000×g supernatant of thymus cells obtained from the rats treated with triamcinolone was significantly less than that from the control rats.
3. The incorporation of phenylalanine, lysine, and proline into microsomal proteins of rat thymus was stimulated by polyuridylic, polyadenylic, and polycytidylic acids, respectively. However, the stimulation of polyuridylic acid to incorporate phenylalanine into proteins of the thymus microsomes obtained from the hormone-treated rats was significantly less than that of thymus microsomes from the control rats.
4. It was suggested from these observations that both the soluble fraction and the ribosomes of thymus cells are involved in the inhibition of protein synthesis in thymus microsomes following the administration of adrenal cortical hormones.抄録全体を表示PDF形式でダウンロード (678K) -
HIROKO NISHIKAWA, ISAMU SHIIO1969 年 65 巻 4 号 p. 523-529
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリー1. When purine requiring mutants of Bacillus subtilis were cultured for 16 hr in a medium containing caffeine, the specific activity of sAMP lyase [EC 4. 3. 2. 2] increased to about twice as much as that when cultured in the absence of caffeine. Other enzymes in the pathway of purine nucleotide biosynthesis, PRPP amidotransferase [EC 2. 4. 2. 14] and IMP dehydrogenase [EC 1. 2. 1. 14], did not increase with the addition of caffeine.
2. Various xanthine derivatives other than caffeine, did not show any effect.
3. The presence of various concentrations of guanosine or adenosine, which repressed the enzyme formation, did not disturb the caffeine effect.
4. In a purine free medium with or without caffeine, time course of the sAMP lyase formation was tested. While caffeine was not effective in an early stage of the cultivation, the specific activities of sAMP lyase increased rapidly after 12 hr cultivation only when caffeine was present.
5. Puromycin, a specific inhibitor of the protein synthesis, inhibited the effect of caffeine. It was concluded that the effect of caffeine was not activation of the enzyme activity.抄録全体を表示PDF形式でダウンロード (422K) -
YOSHIHIRO MIYAKE, KENJI YAMAJI, TOSHIO YAMANO1969 年 65 巻 4 号 p. 531-537
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーBoth crystalline holo- and apo-enzymes of D-amino-acid oxidase [EC 1. 4. 3. 3] from hog kidneys produced antibodies when they were administrated to rabbits for immuni-zation. The presence of about six antigenic sites in the enzyme was demonstrated from the quantitative precipitin test. The antibodies partially inhibited D-amino-acid oxidase. γG-Immunoglobulin for the polo-enzyme (IgGt1) was further fractionated to fragments. Fragments I and II inhibited the enzyme action partially, while Fragment III did not at all. The type of the inhibition was partially noncompetitive with respect to the substrate. Binding of FAD to the apo-enzyme was not inhibited by these fragments. It was thus concluded that there was no distinct difference in the reactions of these two antibodies with the enzyme, and the effect of the antibodies on the enzyme active site was indirect.抄録全体を表示PDF形式でダウンロード (1320K) -
VI. Purification and Properties of the Hog Brain EnzymeKENJI AKI, AKIO YOKOJIMA, AKIRA IOHIHARA1969 年 65 巻 4 号 p. 539-544
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーBranched chain amino acid (valine, leucine and isoleucine)-α-ketoglutarate trans-aminase [EC 2. 6. 1. 6] was purified from hog brain supernatant by DEAE-cellulose, hydroxylapatite and DEAE-Sephadex column chromatographies. The purified enzyme was shown to be a single protein by ultracentrifugation, immuno-double diffusion and agar and acryamide gel electrophoreses. Its s20, W was 3.3. Substrate specificity was limited to the substrates described above. The Km values for the substrates and cofactor were (in mM): 0.56 for leucine, 0.67 for isoleucine, 1.4 for valine, 0.57 for a-ketogluta-rate and 0.0065 for pyridoxal phosphate. 2-Mercaptoethanol activated the enzyme. These properties, except the chromatographic behavior and molecular weight, are very similar to those of the enzyme in hog heart.
Anti-serum against hog brain enzyme inhibited the activity of the homologous enzyme and that of heart enzyme to a lesser extent. Conversely, anti-serum against hog heart enzyme neutralized the activities of both the heart and brain enzymes, though the inhibition of the latter was less. The properties of the two enzymes and their distributions in various hog tissues were compared.抄録全体を表示PDF形式でダウンロード (1122K) -
YOSHIKI TAKESUE1969 年 65 巻 4 号 p. 545-552
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーRabbit intestinal sucrase, which is associated with the microvillous membrane of the mucosal cell, was solubilized with papain [EC 3. 4. 4. 10] and purified by utilizing its reversible adsorption on Sephadex G-200 and gel filtration on Bio-Gel P-300.
The purified enzyme was homogeneous on ultracentrifugation and disc electrophoresis analyses. The sedimentation coefficient (s020, W) of the enzyme was 10.OS and its molec-ular weight was estimated to be approximately 235, 000 by the Archibald method.
It hydrolyzed maltose and α-methylglucoside in addition to sucrose, but not α, α-trehalose. β-Glucosides and α- and β-galactosides tested were not hydrolyzed. Melezi-tose served as a substrate but raffinose did not. Furthermore, the maltase activity present in the homogenate paralleled to the sucrase activity during purification. These findings indicate that the enzyme is a glucosido-sucrase or an a-glucosidase [EC 3. 2. 1. 20].
Other enzymatic properties such as pH optimum, Michaelis constant, activators and inhibitors were studied.抄録全体を表示PDF形式でダウンロード (555K) -
Complete Digestion with Ribonucleases and Construction of Model of Its Secondary StructureSHOSUKE TAKEMURA, MITSUMASA MURAKAMI, MASAZUMI MIYAZAKI1969 年 65 巻 4 号 p. 553-566
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーIsoleucine tRNA from Torulopsis utilis was purified by column chromatography on DEAE-Sephadex. The tRNA was obtained in a purity of about 95%, which is proba-bly one of the purest preparation of tRNA specific for a single amino acid. All of the fragments from the complete digests with pancreatic RNase [EC 2. 7. 7. 16] and RNase T1 [EC 2. 7. 7. 26] were isolated and analyzed mainly by column chromatography. The analyses indicated that this tRNA is composed of 77 nucleotide residues. Thirteen unusual nucleotides are present, including N-(purin-6-ylcarbamoyl)-threonine ribonucleo-tide. It is the first time that this nucleotide has been isolated from a pure specific tRNA.
A model of the unique secondary structure of this tRNA can be constructed from the fragments formed by the two RNases, according to known general features of a clover leaf type of arrangement.抄録全体を表示PDF形式でダウンロード (986K) -
IX. Effect of F-Actin on the Myosin-ATP SystemNAOKAZU KINOSHITA, TOHRU KANAZAWA, HIROFUMI ONISHI, YUJI TONOMURA1969 年 65 巻 4 号 p. 567-579
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリー1. Myosin was treated with NTP by the method described in the preceding paper (2). As mentioned in the preceding paper (2), the rate of ATP-hydrolysis of myosin [ATP : phosphohydrolase, EC 3. 6. 1. 3] in the steady state was only slightly altered by treatment with NTP, but no initial burst of Pi-liberation could be observed with NTP-myosin. The actomyosin type of ATPase activity was inhibited by treatment of myosin with NTP. At low ionic strength and in the presence of MgCl2, no superprecipitation or clearing response could be observed with actomyosin reconstituted from NTP-myosin and F-actin.
2. At high ionic strength and low Mg++ concentration the amount of initial rapid P liberation was more than I mole per 4×105g of myosin; i.e., an extra-burst of Pi-liberation was observed. As mentioned in the preceding paper (2) the initial extra-liberation of hydrogen ion was accompanied by an extra-burst of P liberation. The dependencies of the amount of initial extra-burst of hydrogen ion-liberation on the ATP concentration were measured in the presence of various Mg++ concentrations. They were in good agreement with those of the amount of decrease in light-scattering intensity of reconstituted actomyosin induced by ATP. The rate of initial rapid hy-drogen ion-liberation was essentially equal to that of decrease in light-scattering.
3. The dependency of actomyosin ATPase on Mg++ concentration was studied. The ATPase activity increased with the concentration of Mg++ until the concentration of added MgCl2 reached 5 pss. At higher concentrations the activity decreased gradually with increasing Mg++ concentration. The rate of superprecipitation of actomyosin in the presence of 10μM MgCl2 was much faster than that in the presence of 1mM MgCl2.
4. When F-actin was added to the myosin-ATP system at various times (10-120 see) after mixing myosin with ATP, the initial rapid ATP-splitting, seen when ATP was added to actomyosin, was not detected, but only the steady state actomyosin ATPase was observed.
5. Based on the results given in this and the preceding paper (2), the following reaction mechanism is proposed for the function of F-actin in the myosin (E)-ATP (S) system:
F-actin inhibits the conversion of phosphoryl myosin, _??_to the weakly reactive ADP .P myosin-phosphate-ADP complex, _??_When the myosin component of actomyosin ADP is converted to the myosin-phosphate-ADP complex by ATP, reconstituted actomyosin dissociates to myosin and F-actin at high ionic strength. But at low ionic strength the presence of F-actin accelerates the decomposition of the myosin-phosphate-ADP complex to myosin, ADP and Pi.抄録全体を表示PDF形式でダウンロード (3934K) -
I. Purification and Properties of Cytochrome b-555YOSHIIE OKADA, KAZUO OKUNUKI1969 年 65 巻 4 号 p. 581-596
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリー1. Cytochrome b-555 was highly purified from larvae of the housefly, Musca domestica L. The absorption spectra of the purified cytochrome b-555 showed peaks at 358-360, 414, and 530mμ in the oxidized form, and at 424, 528, and 555mμ in the reduced. The cr-band of the reduced form was asymmetric at room temperature and at neutral pH, but split into two distinct peaks at 552 and 556mμ at liquid nitrogen temperature. The purified cytochrome contained protoheme as the prosthetic group and combined neither with carbon monoxide nor with cyanide.
2. In the ultracentrifugation, the cytochrome preparation showed a symmetrical pattern and its sedimentation coefficient, s20, W, was 1.43S. The molecular weight was determined to be 13, 700 by the gel filtration method.
3. Cytochrome b-555 was reduced readily by sodium dithionite, slowly by cysteine, and anaerobically by ascorbate, but was not reduced non-enzymatically by NADH. The reduced form was oxidized by potassium ferricyanide, beef heart ferricytochrome c, and very slowly by air (the apparent first-order velocity constant was 0.0046 sec-1). The midpoint redox potential (E'0) of cytochrome b-555 was +0.006 volt at pH 7.0 and at 12°C.
4. Purified cytochrome b-555 was reduced by NADH in the presence of the larval microsomal fraction or NADH-cytochrome b5 reductase [EC 1. 6. 2. 2] purified from rat liver microsomes. The reaction was inhibited neither by antimycin A nor by rotenone as in the case of microsomal NADH-cytochrome bs reductase systems of housefly larvae and mammalian liver.
5. Purified cytochrome b-555 seemed to be a solubilized form of cytochrome b5 which existed both in the mitochondrial and microsomal fractions prepared from the larvae. Solubilization of larval cytochrome b5 from the cytoplasmic membranes was discussed.抄録全体を表示PDF形式でダウンロード (2161K) -
SHOGO ICHII, NAGASUMI YAGO1969 年 65 巻 4 号 p. 597-601
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリー1. Role of the hormones of adrenal and testis in regulating hepatic microsomal aminopyrine N-demethylase activity was studied in male rats that were subjected to adrenalectomy, castration or both with or without replacement therapy.
2. Corticoid and androgen were found to regulate different fractions of the amino-pyrine N-demethylase activity, and level of this enzyme activity was shown to be mainly maintained by corticoid and androgen in intact male rats. Size and rate of decrease of the corticoid- and androgen-dependent fractions were almost the same to each other.
3. Phenobarbiturate-inducible fraction of the aminopyrine N-demethylase activity was quite independent of the adrenal and testicular control.
4. NADPH-cytochrome c reductase, NADH-cytochrome c reductase, and cytochromes P-450 and b5 of microsomal fraction were determined in livers of the operated rats.抄録全体を表示PDF形式でダウンロード (318K) -
I. Partial Purification and Chemical PropertiesKAYOKO IKEDA, FUJIO EGAMI1969 年 65 巻 4 号 p. 603-609
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリー1. Pyocin R receptor substance was prepared from Pseudomonas aeruginosa P11 (pyocin R sensitive strain) by trichloroacetic acid extraction, nuclease digestion and pronase digestion. Most of the receptor substance was precipitated by ultracentrifugation.
2. This substance was mainly composed of lipopolysaccharide and contained glucose, rhamnose, glucosamine, galactosamine, heptose and a sugar giving a positive reaction with thiobarbituric acid in the carbohydrate moiety. It contained a small amount of amino acids.
3. The receptor activity decreased on chloroform-methanol extraction of the lipid, on alkali treatment or on periodate oxidation.
4. The TCA extract from the pyocin R resistant mutant (P11 Rr) had no receptor activity and no corresponding precipitates could be obtained from the extract by ultra-centrifugation.
5. The TCA extraction method was more effective than the phenol method for preparation of lipopolysaccharide with receptor activity.抄録全体を表示PDF形式でダウンロード (430K) -
MIKI GOTO, ATSUSHI SAKURAI, KYUJI OHTA, HIROSHI YAMAKAMI1969 年 65 巻 4 号 p. 611-620
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーFür den Harnfarbstoff Urothion wurde durch Abbaureaktionen and die Synthese einer Modellverbindung die Struktur eines 7-Amino-2-(α, β-dihydroxyäthyl)-3-methylthio-5-hydroxythieno[3, 2-g]pteridins vorgeschlagen.抄録全体を表示PDF形式でダウンロード (1591K) -
TAKAO KODAMA, TAKESHI MORI1969 年 65 巻 4 号 p. 621-628
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーFrom acetone-dried powder of aerobically grown cells of a denitrifying bacterium, van Niel strain of Pseudomonas stutzeri, a new c-type cytochrome, which exhibits absorp-tion spectra and chemical properties different from those of other two c-type cytochromes contained in the same organism, could be obtained in highly purified state and was referred to as cytochrome c-552, 558.
In 0.05M phosphate buffer, pH 7.0, absorption maxima of reduced cytochrome c-552, 558 are at 421, 526, and 558mμ with a shoulder at 552mμ. The rnillimolar extinction coefficient (based on the heme content) at 558 mp is very low (εmM=18.8). In the presence of alkali, urea and alcohols, the reduced spectrum is converted to that of typical bacterial c-type cytochrome : absorption maxima appear at 418, 524, and 552mμ and the absorbancy at each maximum is greatly intensified. The spectrum thus changed is reversed to its original state on removing these reagents.
Reduced cytochrome c-552, 558 is oxidized in air and reacts with CO even at neutral pH. Oxidized hemoprotein can be reduced by dithionite, but not by borohydride, ascorbate or cysteine.
The molecular weight of cytochrome c-552, 558 estimated from centrifugal data or a gel-filtration method is about 70, 000 and its minimum molecular weight calculated from the heme content is about 37, 000, indicating that it contains two hemes per molecule.
Cytochrome c-552, 558 was found to be also contained in the soluble fraction on disruption of washed fresh cells by sonic oscillation followed by centrifugation at 105, 000×g for 1 hr.抄録全体を表示PDF形式でダウンロード (1539K) -
YOSHIO IMAI, KAZUKO IMAI, KIYOSHI IKEDA, KOZO HAMAGUCHI, TAKEKAZU HORI ...1969 年 65 巻 4 号 p. 629-637
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーThe circular dichroism (CD)** of cytochrome cc' from Rhodospirillum rubrum and cytochrome c' from Rhodopseudomonas palustris was measured in the 205-500 mμ region under various conditions where the cytochromes show different types of absorption spectra. At pH 7 the oxidized form of cytochrome cc' exhibits a positive CD extremum at 406mμ in the Soret region, a broad negative CD band from 325 to 360mμ in the o region, and positive extrema between 260 and 285mμ in the aromatic region. The CD spectrum in the far-ultraviolet region exhibits negative extrema at 222 and 207 mμ, which are characteristic of the α-helix. The helical content is calculated to be about 63%. The CD spectra in the Soret, δ, and aromatic region can be changed by the environment concomitant with changes in the absorption spectra. Changes in the CD spectrum in the aromatic region, which are parallel to those in the Soret region, indicate that the CD bands in the aromatic region reflect the environment of the heme groups. Addition of 25% 2-propanol to cytochrome cc', which results in the conversion of the absorp-tion spectrum and the CD spectrum in the Soret, δ, and aromatic regions from the neutral to the alkaline type, causes essentially no change in the CD spectrum in the far-ultraviolet region. In the presence of 2M KCl at pH 12.3, where the absorption spectrum is of the intermediate type, the CD spectrum in the far-ultraviolet region is also the same intermediate type. In the absence of KCI the negative band at 222mμ is greatly diminished at pH 12.3. Treatment with 6M urea for 20 hr at room tem-perature at pH 7 causes considerable destruction of the helical structure but produces only a small change toward the alkaline type in the Soret, δ, and aromatic regions. Only slight changes are found in the whole wavelength range when the sample is examined immediately after the addition of urea. Similar results are obtained on cytochrome c'.
These findings suggest that the change in the absorption spectrum from the neutral to the alkaline type is not necessarily accompanied by a change in the gross helical conformation of the protein moiety which might protect the special environment of the heme.抄録全体を表示PDF形式でダウンロード (645K) -
OSAO ADACHI, HIDEAKI YAMADA1969 年 65 巻 4 号 p. 639-640
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (146K) -
YASUKO WATANABE, HISAKO TAKEDA, BONRO KOBAYASHI1969 年 65 巻 4 号 p. 641-643
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (174K) -
TARO HAYAKAWA, MASAHIKO KOIKE1969 年 65 巻 4 号 p. 645-648
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (220K) -
HIDEHIKO MUKASA, YOSHIAKI NOSOH, TETSUO SATO1969 年 65 巻 4 号 p. 649-650
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (161K) -
MASAMICHI TAKAGI, TATSUO TANAKA, KIKUO OGATA1969 年 65 巻 4 号 p. 651-653
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (183K) -
MITSUO HATTORI, JINICHI ONISAWA, MIKI GOTO1969 年 65 巻 4 号 p. 655-657
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (197K) -
SHUICHI HASHIMOTO, MASAZUMI MIYAZAKI, SHOSUKE TAKEMURA1969 年 65 巻 4 号 p. 659-661
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (226K) -
KUNIO YAGI, MORIMITSU NISHIKIMI, NOBUKO OHISHI, KEITARO HIROMI1969 年 65 巻 4 号 p. 663-666
発行日: 1969/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (1098K)
- |<
- <
- 1
- >
- >|