巻号一覧
54 巻, 4 号
選択された号の論文の13件中1～13を表示しています
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The Kinetic Analysis of Hemolysis by Clostridium perfringens (Cl. welchii) α-Toxin (Phospholipase C)HIROH IKEZAWA1963 年 54 巻 4 号 p. 301-311
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリー1. The analysis based upon enzyme kinetics was made on the mechanism of hemolysis of sheep erythrocytes by Clostridium perfringens α-toxin (phospholipase C).
2. The mechanism of substrate breakdown which led to hemolysis was shown to be
M+S_??_MS
E MS_??_E'
E'+MS_??_MS_??_E'+MP (or E'+M+P)
a. When the substrate concentration was reduced to a critical value, the hemolytic reaction seemed to begin.
b. The induction period was composed of two latent periods t1 and t2. The lag t1 was independent of, while the lag t2 was inversely proportional to, the enzyme concentration.
3. When excess antitoxin was added to the reaction medium before E'MS was formed, a complete inhibition occurred, while a partial inhibition was observed when it was added after E'MS was formed.
In the presence of Ba_??_, the lag t1 was not affected while the lag t2 was prolonged. The apparent rate constant K was decreased in the presence of Ba_??_.
5. A new method for calculation of HDso was presented.
The author is indebted to Dr. R. Murata, Chief of the Department of Bacteriology II, the National Institute of Health, and Dr. D. Mizuno, Professor of the University of Tokyo for their helpful advices.
He is also indebted to Dr. H. Kusama for the gift of antistreptolysin O serum and wishes to express his thanks to Dr. A. Yamamoto, Dr. M. Miwa and Miss M. Tsukamoto, of the Department of Bacteriology II, the National Institute of Health, for their useful collaborations.抄録全体を表示PDF形式でダウンロード (727K) -
KAYOKO IKEDA, HIROMI YAMADA, SHOZO TANAKA1963 年 54 巻 4 号 p. 312-316
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリー1. A microorganism which actively utilizes taurine as a sole carbon and nitrogen source, was isolated and identified as a species of Agrobacterium.
2. The intact cells of this bacterium degrade taurine as follows:
NH2CH2CH2SO3H+2O2→H2SO4+NH3+CO2+(CH2O)
3. The cell free extract of this bacterium liberates amino nitrogen of taurine as ammonia, and sulfonyl group as sulfate. The deamination seems to precede the desulfona-tion.抄録全体を表示PDF形式でダウンロード (364K) -
YUICHI SHIOBARA, KAZUKO ITAGAKI1963 年 54 巻 4 号 p. 317-321
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリー1. With partially purified polynucleotide phosphorylase extracted from Azotobaster vinelandii, the formation of poly AG and poly UG was investigated. The reaction became difficult with increasing amount of GDP. But the guanine content in the polymer was found to be larger than that of reaction mixture.
2. By the addition of ATP, the formation of poly AG was greatly enhanced, but the product was poorer in guanine content.
3. Urea was found to be effective in protecting the polymer from degradation.
Various polymers such as poly A, poly C, poly U and poly UG were shown to be successfully prepared in its presence with relatively crude enzyme preparation, though the yield of poly A was somewhat low. Combined addition of AMP and urea was found to be a convenient method for poly A synthesis.
The authors wish to express their sincere thanks to Prof. F. Egami for his guidance and encouragement during this work. They are also indebted to Dr. J. Koyama for his valuable suggestions to this study.抄録全体を表示PDF形式でダウンロード (333K) -
YOICHI OHTAKA, KATSUHISA UCHIDA, TAKUO SAKAI1963 年 54 巻 4 号 p. 322-327
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーRNase was extracted from baker's yeast by autolysis and chromatographed on CM-cellulose column. The highly purified enzyme has following properties.
1. The enzyme is heat sensitive and its pH optimum is 7.5.
2. It depolymerizes yeast RNA completely to acid soluble products, which are separated on Dowex-l column and confirmed to be 3'-mononucleotides. However, it splits neither DNA, dephenylphosphate nor 2, 3'-cyclic nucleotides.
3. Although the enzymatic reaction proceeds in absence of phosphate, it is activated remarkably by 0.1 M phosphate. The enzyme is completely inhibited by zinc ion at 10-4M but not by magnesium ion and the inhibition is reversed by the addition of EDTA, citrate or histidine.
The authors wish to express their thanks to Drs. K. Sakaguchi, S. lida and Y. Ikeda for their kind guidances and suggestions. They are also grateful to Dr. K. Asano for gift of cyclic nucleotides.抄録全体を表示PDF形式でダウンロード (363K) -
Isolation an Identification of O-α-Glucopyranosyl-(1→4)-phenyl-6-O-acetyl-α-D-glucopyranosideTOKUJI IKENAKA1963 年 54 巻 4 号 p. 328-333
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリー1. Phenyl monoacetyl-α-maltoside was isolated from the mixed maltoside which was obtained by deacetylation of phenyl hept-acetyl-α-maltoside with methanol-ammonia, by the enzymatic hydrolysis with taka-amylase A followed by cellulose column chromatography.
2. The position of acetyl group in phenyl monoacetyl-α-maltoside was identified to be position 6 in Fig. 8, and the monoacetyl derivative was O-α-D-glucopyranosyl-(l→4)-ph e nyl-6-O-acetyl-α-D-glucopyrano side.
3. The monoacetyl-maltoside was not hydrolyzed by the action of TAA and also did not inhibit the enzymatic hydrolysis of phenyl α-maltoside by TAA, therefore, it might be concluded that the hydroxyl group in position 6 plays an important role for the formation of Michaelis complex of TAA and the substrate.
The author wishes to express his thanks to professors S. Akabori and Y. Matsushima of Osaka University for their valuable advices and discussions, and Dr. K. Hiroumi, University of Osaka Prefecture, for his kind supply of glucoamylase.抄録全体を表示PDF形式でダウンロード (418K) -
KOYU NARUMI, TORU TSUMITA1963 年 54 巻 4 号 p. 334-338
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリー1. Polymannophosphate, a phosphoryla-ted polysaccharide, was found in a hot ethanol extract of Mycobacterium tuberculosis (bovine type), BCG and was purified by column chro-matography.
2. The chemical composition was man-nose and phosphate in a molar ratio of 2.6:1.
3. The material was non-dialyzable and was electrophoretically homogeneous.
4. The content of the material in the cell was higher in younger culture than that of aged.
5. Another two kinds of compounds composed of mannose and phosphate were also found in dialyzable form and the relation with polymannoposphate was discussed.
The authors wish to thank Prof. T. Yamakawa for useful discussions. They are also grateful to Dr. T. Hashimoto for his supply of Mycobacteria and to Miss S. Kawamura for the elemental analyses.抄録全体を表示PDF形式でダウンロード (385K) -
TOSHIO ANDOH, SHUNJI NATORI, DEN'ICHI MIZUNO1963 年 54 巻 4 号 p. 339-348
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリー1. When uracil-starved E. coli cells were fed with (2-C14) uracil for 30 seconds at 37°C, it was incorporated into RNA fraction which has a base composition identical to that of E. coli DNA. This rapidly labeled RNA was treated as messenger RNA of E. coli.
2. Selective degradation of the m-RNA on the washed ribosome occurred in the presence of inorganic phosphate (50 mM).
3. By analysis of degradation products of the RNA produced during the incubation in the presence of inorganic phosphate, nucleoside 5' monophosphate and diposphates were isolated as the main labeled nucleotides and the secondary conversion of 5' monophos-phate to diphosphate was not detected.
4. It was concluded that both polynuc-leotide phosphorylase and an as yet uncharac-terized venom type phosphodiesterase stimu-lated by Pi are involved in the degradation of m-RNA according to the following equations;
RNA+n Pi→n XDP→n XMP (5') RNA→n XMP (5')
Overall reaction kinetics was found to be of the second order.
5. Discussion was given that the apparent selective degradation of m-RNA was partly due to its physicochemical state on the ribosome rather than to its peculiar character.
This work was financially aided in part by the Research Grant from the Ministry of Education of Japan. The authors are grateful to Prof. Tsuboi of our Faculty for the generous supply of nucleoside diphosphates and polynucleotide phosphorylase of Azotobacter ainerandii. Thanks are also due to Mr. T. Takano of our Department for the technical assistance in the purification of E. coli acid phosphatase.抄録全体を表示PDF形式でダウンロード (751K) -
I. Über die Isolierung des Histidins und seiner Derivate aus der Muskulatur von Anguilla japonica, Neothynnus macropterus, Gymnothorax kidako, Ophicephalus argus and Cypselurus agooKAZUYOSHI HORISAKA1963 年 54 巻 4 号 p. 349-354
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーAus den verschiedenen Fischmuskeln wurden Histidin und seine Derivate kristallinisch dargestellt.
1. Aus der Muskel mit Haut (7.5kg.) von Anguilla japonica wurden 15.7g. Carnosin kristallinisch dargestellt, und es wurde durch Analyse und Papierchromatographie identifiziert.
2. Aus der Muskel (4kg.) von Neothynnus macrupterus wurden 14.7g. Anserin und 0.9g. Histidin isoliert, und sie wurden durch die erwähnten Methoden identifiziert.
3. Aus der Muskel (4kg.) von Gymnothorax kidako wurden 0.07g. Histidin kristallinisch erhalten.
4. Aus der Muskel (4kg.) von Ophicephalus argus wurden 0.4g. Histidin kristallinisch dargestellt. Weiter ist die Anwesenheit von 3-Methylhistidin papierchromatographisch erwiesen.
5. Aus der Muskel (4kg.) von Cypselurus agoo wurden 12.5g. Histidin kristallinisch dargestellt.
Herrn Prof. Dr. Sh. Tsunoo spreche ich hiermit für seine freundliche Anleitung bei der Ausführung dieser Arbeit meinen verbindlichsten Dank aus. Fräulein S. Takahashi möchte ich für wertvolle tech nische Hilfe danken.抄録全体を表示PDF形式でダウンロード (391K) -
Die Isolierung und Identifizierung des Histidins und AnserinsSHIGERU TSUNOO, KAZUYOSHI HORISAKA, MASAKAZU KAWASUMI, KAZUE ASO, SHIG ...1963 年 54 巻 4 号 p. 355-362
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリー1. Aus dem Hirn des Huhns (10kg.) wurden 0.4g. Anserin und 0.4g. Histidin kristallinisch dargestellt, und sie wurden durch Salzbildung, Analyse und Papierchro-matographie identifiziert.
2. Durch die Säulen- und Papierchro-matographie zeigte sich, dass Asparaginsäure, Glutaminsäure, Serin, Threonin, Prolin, Glycin, Alanin, β-Alanin, γ-Aminobuttersäure, Valin, Phenylalanin, Leucin, Methionin, Tryptophan, Isoleucin, Lysin und Arginin in freier Form vorkommen.
Fräulein S. Takahashi möchten wir für wertvolle technische Hilfe danken.抄録全体を表示PDF形式でダウンロード (550K) -
Die Isolierung und Identifizierung des Histidins und AnserinsSHIGERU TSUNOO, KAZUYOSHI HORISAKA, SHIGERU SASAKI, KAZUE ASO, SHIGERU ...1963 年 54 巻 4 号 p. 363-368
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリー1. Aus dem Auge des Huhnes (10kg.) wurden 1.1g. Anserin und 0.02g. Histidin kristallinisch isoliert.
2. Die obengenannten Substanzen wurden durch Salzbildung, Elementaranalyse und Papierchromatographie respektive identifiziert.
3. Durch Säulen- und Papierchromatograghie zeigte rich, dass Asparaginsaure, Glutaminsäure, Serin, Threonin, Prolin, Glycin, Alanin, β-Alanin, γ-Aminobuttersäure, Valin, Phenylalanin, Leucin, Lysin und Arginin in freier Form vorkommen.Fräulein S. Takahashi möchten wir für wertvolle technische Hilfe danken.抄録全体を表示PDF形式でダウンロード (361K) -
LIV. Studies on the Bile of CarpTAKAHIKO HOSHITA, SADAO NAGAYOSHI, TARO KAZUNO1963 年 54 巻 4 号 p. 369-374
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリー1. Bile salts of carp, Cyprinus carpio, when hydrolyzed with trichloroacetic acid in dioxane solution yielded cyprinol as a chief component, which is believed to be 3α, 7α, 12α, 26, 27-pentahydroxycholestane on the baisis of the nuclear magnetic resonance spectroscopic data and on the fact that 3α, 7α, 12α-trihy-droxy-26, 27-epoxycholestane was derived from cyprinol sulfate.
2. The presence of cholic acid in carp bile was confirmed.
The authors wish to thank Dr. K. Takeda, Director of Shionogi Research Laboratory, Osaka, for his generous help in determination and interpretation of nuclear magnetic resonance spectra, and Prof. G. A. D. Haslewood, Guy's School of Medicine, London, for his notification of unpublished work and for his helpful discussion.抄録全体を表示PDF形式でダウンロード (313K) -
SHIGERU NAKAYAMA, SHIZUKO AMAGASE1963 年 54 巻 4 号 p. 375-377
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (1502K) -
TORAO ISHIDA, KIN-ICHIRO MIURA1963 年 54 巻 4 号 p. 378-379
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (139K)
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