巻号一覧
39 巻, 4 号
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HARUTADA NINOMIYA, SUZUOKI-ZIRO1952 年 39 巻 4 号 p. 321-331
発行日: 1952年
公開日: 2008/11/18
ジャーナル フリーUsing the suspension of Trichomonas vaginalis, a parasitic protozoon, the carbohydrate metablism were studied manometrically both under aerobic and anaerobic conditions.
1. The effects of pH, the concentration of NaCl and oxygen tension upon the respiration were preliminarily investigated.
2. Of 11 substrates tested, only glucose, maltose and lactate were oxidized by T. vaginalis.
3. The respiration was highly sensitive to -SH inhibitors (p-chloro-mercuribenzoate, monoiodoacetate and arsenite) and also susceptible to fluoride. Cyanide, azide, malonate and arsenate had no inhibitory effect.
4. High oxygen tension inhibited almost completely the increased oxygen uptake in the presence of glucose, while it increased autore-spiration. It was concluded that the inhibitory effect of oxygen depended mainly on the accumulation of hydrogen peroxide formed during glucose oxidation. In contrast to T. foetus (Qkat=800), T. vaginalis had no catalase activity (Qkat=0).
5. Under anaerobic condition, T. vaginalis evolved not only carbon dioxide, but some other gases. The gas evolution increased by the ad-dition of glucose or pyruvate, but rather decreased by -SH inhibitors.
A glance was given upon the comparative biochemistry of trichomo-nerds, and the results obtained were also discussed from the view point of the evolutional adaptation from “free-living” to “parasitic” existence of these organisms.抄録全体を表示PDF形式でダウンロード (681K) -
KENTARO TANAKA, KEN'ICHI TAKEDA1952 年 39 巻 4 号 p. 333-337
発行日: 1952年
公開日: 2008/11/18
ジャーナル フリーZaffaroni's chromatographic method of developing Girard T hydrazone of ketosteroids was applied to ketobile acids, and similar results were obtained, confirming its applicability in this field.
1. After hydrazone formation, 14 kinds of ketobile acids syn-thetically prepared were chromatographically developed by n-butanol: iso-propanol: water (6:2:1) mixture by which separation was effected.
2. By this method, the ketobile acids possessing a ketone group in the 3-position are either totally undetected or they behave like the substances possessing one ketone group less than they actually have.
3. The effect of other polar groups than ketone groups, was found to be governed by a certain rule.抄録全体を表示PDF形式でダウンロード (298K) -
II. THE FATE OF ACETATE AND PROPIONATE IN RESPIRATION AND PHOTOSYNTHESISAKIRA TSUKAMOTO1952 年 39 巻 4 号 p. 339-347
発行日: 1952年
公開日: 2008/11/18
ジャーナル フリー1. Using a non-sulfur purple bacterium, Rhodobacillus palustris, the availability of various fatty acids as the hydrogen donor for the photosynthetic CO2 reduction was investigated. The oxidation velocity of each fatty acid, as it is reflected in the rate of CO2-uptake, was found to be constant up to a very low concentration of the acid, indicating
that the Michaelis' constant of the reactions are appreciably small. The rate at saturating concentration of substrate increased with the number of carbon atom in the acid molecule up to C9, and then gradually de-creased till it became zero at C17-C18. The ratio: (net CO2-uptake in mole)/(fatty acids used in mole) also increased with the number of the carbon atom in the acid molecule. These findings are essentially similar to those made previously in the comparative study of the avail-ability of various fatty acids as substrate of respiration by the same organism. A distinct difference lies, however, in the fact that the photo-synthetic activity of bacterial cells is markedly suppressed by the effect of higher fatty acids which could be readily used as the substrate of res-piration.
2. Per Oxidation of one mole each of acetate and propionate, 0.24 and 0.49 moles, respectively, of CO2 were reduced photosynthetical. ly. Previous experiments showed that in the respiratory process these acids were oxidized with the consumption of 0.5 and 1.0 moles, respective-ly, of O2 and indeed without the, formation of CO2
3. Attempt was made to identify qualitiatively the products formed from acetate and propionate in the process of respiration and photo-synthesis. It was found that in respiration succinate, probably ac-companied by fumarate was formed from acetate, and lactate and pro-bably pyruvate from propionate. On the other hand, the products formed in photosynthesis were found to be succinate from acetate and lactate from propionate.
4. These data were interpreted as indicating that the pathway of fatty acid oxidation by the organism tested is essentially the same in photosynthesis and respiration, and that acetate and propionate are oxidized according to the following fromulae:
Acetate-H→1/2 Fumarate
Propionate-H→(Acrylate)+H2O→Lacatate-2H→Pyruvate
It is my great pleasure to thank Prof. Dr. H. Tamiya for his kind guidance and encouragement throughout this work. This work was supported by a grant from the Scientific Research Fund of the Ministry of Education which is gratefully acknowledged抄録全体を表示PDF形式でダウンロード (572K) -
TOMOICHI KUSUNOKI1952 年 39 巻 4 号 p. 349-355
発行日: 1952年
公開日: 2008/11/18
ジャーナル フリーSpectrophotometric analysis of the binding of dye by proteins has yielded the following results.
1. Human serum albumin shows two step combination reaction with methyl orange, while horse, bovine, hen, and rabbit serum albumins show only one step reaction.
2. Albumin concentration and therefore the A/G ratio of normal and various pathological sera can be determined fairly accurately by spectrophotometric determination of bound dyes except in the case of hepatic insufficiency and nephrosis.
3. Serum proteins in severe hepatic insufficiency bind less dye ions than can be accounted for on the basis of albumin concentration.
The author expresses his cordial thanks to Prof. K. Kodama and Assist. Prof. H. Yoshikawa for their kind guidance and also to Dr. H. Hirai and Mr. K. Shimao for their helpful advice and valuable discussion throughout the course of these investigations.
The author is greatly indebted to Dr. K. Sakaguchi, Chief of the First Tokyo National Hospital, Dr. K. Miyoshi and Dr. T. Sasakiin Tokyo University Hospi-tal, and Dr. F. Yanagisawa for their kind supply of normal and pathological sera used in this study.抄録全体を表示PDF形式でダウンロード (429K) -
IV. ON THE FORMATION OF 630-COMPOUND IN THE REACTION SYSTERM OF PYRIDINE-HEMIN-ASCORBIC ACID-HYDROGEN PEROXIDEKOOZOO KAZIRO, GORO KIKUCHI1952 年 39 巻 4 号 p. 357-366
発行日: 1952年
公開日: 2008/11/18
ジャーナル フリー1. The final product of pyridine-hemin in the reaction system of pyridine-hemin, ascorbic acid and H2O2 is 630-compound. No by-product formation is observed in the system unless a great excess of H2O2 is used.
2. The concentration-absorption curve for the 630-compound was determined; εmol at 630μm being equal to 0.82×104.
3. The amount of 630-compound formed corresponds to the amount of H2O2 when that of ascorbate is kept constant, while it cor-responds to that of ascorbate at a given concentration of H2O2
. 4. In the formation of 630-compound, the concentration of H2O2 plays a greater part than that of ascorbate.
5. The optimum pH for the formation of 630-compound is 9.3.
6. The 630-compound is supposed to be most active as one of the peroxidatic catalysts among the hemin derivatives existing in the system.
The present investigation was supported by the subsidy of the Science Research Fund of the Ministry of Education, for which we express our hearty thanks.抄録全体を表示PDF形式でダウンロード (627K) -
I. THE INFLUENCE OF THE ADMINISTRATION OF SULPHUR CON-TAINING AMINO ACIDSTAKEKAZU KÔSAKI, TADAO IKEDA1952 年 39 巻 4 号 p. 367-379
発行日: 1952年
公開日: 2008/11/18
ジャーナル フリー1. Sulphur containing amino acids in general increase the metal-loporphyrin of tissues markedly, there being no decrease in the porphyrin of tissues.
2. The increase of tissue metalloporphyrin becomes most marked at the end of a certain time after the administration and then it gradually decreases.
3. The change in tissue metalloporphyrin takes place most marked-ly and rapidly with cysteine and least markedly and slowly with methio-nine.
4. With cysteine the increase in tissue metalloporphyrin is most marked when given in such doses as 0.05mg./100 g. and with cystine and methionine 0.1mg/ 100 g. and the change is less marked when they are given in larger doses.
5. At the end of 24 hours after the administration of cystine or cysteine in such a dose as 5 mg./100 g. liver metalloporphyrin decreases beyond the normal value and the decrease is more marked with cysteine.
6. Repeated administration of cystine in dose of 0.5 mg./100 g, causes often the decrease of liver metalloporphyrin.
7. The repeated administration of methionine even in such a large dose as 6mg./100 g. causes no decrease of liver metalloporphyrin.
8. The above results indicate that the physiological action of sulphur containing amino acids manifests itself through the change in the metalloporphyrin of tissues.抄録全体を表示PDF形式でダウンロード (883K) -
II. THE INFLUENCE OF THE ADMINISTRATION OF ε-AMINO CAPROIC ACID AND RELATED SUBSTANCESTAKEKAZU KÔSAKI, TADAO IKEDA, YASUMICHI NODA1952 年 39 巻 4 号 p. 381-387
発行日: 1952年
公開日: 2008/11/18
ジャーナル フリー1. ε-amino caproic acid increases markedly the metalloporphyrin of various tissues other than intestine, skin, lungs, cerebrum and bone and decreases the porphyrin of whole blood and the porphyrin bodies of urine.
2. Caproic acid is, in general, a factor that decreases more or less tissue metalloporphyrin.
3. In case, an amino group is substituted to α- or ε-position of caproic acid, marked difference is observed in the effect on tissue metal-loporphyrin according to the position of the substitution. The sub-stitution to ε-position causes an increase of the metalloporphyrin and the substitution to α-position bring about an increase in the cases of a half number of tissues investigated and a decrease in the cases of other half.
4. The effect of α, ε-diamino caproic acid on tissue metallopor-phyrin is either the algebraic sum of the effects of α-substitution and of e-substitution or depends mainly upon the effect of either one, especially on that of α-substitution.
5. There is such an aspect in the physiological action of ε-amino caproic acid and its related substances as to be explained by the change in the tissue metalloporphyrin.抄録全体を表示PDF形式でダウンロード (435K) -
TSUTOMU KOJO1952 年 39 巻 4 号 p. 389-392
発行日: 1952年
公開日: 2008/11/18
ジャーナル フリー1. Pteroylglutamic acid inhibited the regeneration of retinene1 from vitamin A1.
2. The yield of retinene1 and vitamin A1 was increased by adding DPN or DPN and FAD to the enzyme system.
3. The yield of retinene1 and vitamin A1 was decreased in phlo-rhizinized frogs.抄録全体を表示PDF形式でダウンロード (242K) -
III. GLOBOSIDE, THE SUGAR-CONTAINING LIPID OF HUMAN BLOOD STROMATAMIO YAMAKAWA, SHIZUE SUZUKI1952 年 39 巻 4 号 p. 393-402
発行日: 1952年
公開日: 2008/11/18
ジャーナル フリー1. A glycolipid, designated globoside was isolated from Iyophilyzed human blood stroma.
2. Gooboside contained chondrosamine instead of hemataminic acid.
FIG. 4. Absorption curves of carbazole reaction with various mixtures of glucose and galactose. (I) Pure glucose. (II) Glucose 3, galactose 1. (III) Glucose 1, galactose 1. (IV) Glucose 1, galactose 3. (V) Pure galactose.
FIG. 5. Ratio of extinctions at 540mμ and 440mμ in the absorption curves of Fig. 4.
FIG. 6. Absorption curves with globoside (A) and hematosidc (B).
3. Glucose was found in both globoside and hematoside but its content was much smaller than that of galactose.
4. The probable structure of globoside was proposed.抄録全体を表示PDF形式でダウンロード (1409K) -
SHOSHI OTA1952 年 39 巻 4 号 p. 403-408
発行日: 1952年
公開日: 2008/11/18
ジャーナル フリー1. In phlorizinized dogs, DL-allothreonine, DL-threonine and DL-methionine were all capable of yielding glucose.
2. The amino acids administered to fast normal rats were trans-formed into liver glycogen though the amount of glycogen was not so large but significant 6 or 9 hours after its administration.
3. In the urines of dogs and rats a certain amount of the admini-stered amino acid was excreted. In the case of DL-allothreonine and
DL-methionine, the racemic form, while in that Of DL-threonine more D-form than L-form was found.
The author wishes to thank Prof. R. Hirohata for his advice and encouragement during this study.抄録全体を表示PDF形式でダウンロード (368K)
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