巻号一覧
62 巻, 4 号
選択された号の論文の18件中1～18を表示しています
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TOHRU MARUNOUCHI, TAKESHI MORI1967 年 62 巻 4 号 p. 401-407
発行日: 1967/10/25
公開日: 2011/01/25
ジャーナル フリーSome properties of a particle-bound ATPase [EC 3.6.1.3] activityin T. thiooxidans was studied. The presence of Mg++is required forthe activation of the enzyme, and Ca++ can also activate the enzymea little. In the presence of Mg++ the addition of Ca++ rather inhibits the activity. The enzyme activity is not stimulated by Na+ and K+ but is markedly stimulated by S03--. Further investigations have suggested that the enzyme preparation contains at least two different components: one requires Mg++ alone for its activation, and the other requires Mg++ together with S03-- and the optimam pH of the former is about pH 9 and the latter, which we call “sulfite-dependent ATPase”, has its optimum at pH7.5 to 8.0. Most part of the reaction product from ATP is ADP, and especially in the early stage of incubation none of nucleotides other than ADP is detected.
The enzyme preparation exhibits a certain, if rather low, activity to hydrolyze ADP but this activity is indifferent to the addition of S03--. PPi is also hydrolyzed by the same preparation and this pyrophosphatase [EC3.6.1.1] activity is stimulated by S02--to a small extent.β-Glycerophosphate is not hydrolyzed.
From these facts it was concluded that the sulfite-dependent ATPase of T.thiooxidans splits the terminal phosphate group from ATP.抄録全体を表示PDF形式でダウンロード (1062K) -
MUTUMI MURAMATSU, YOSHIE HAYAKUMO, SETSURO FUJII1967 年 62 巻 4 号 p. 408-418
発行日: 1967/10/25
公開日: 2011/01/25
ジャーナル フリー4-Aminobutyl and 6-aminohexyl butyrate, and hexyl Nα-benzoyl-D-lysinate were prepared, and their inhibitory effects on trypsin [EC3.4.4.4] and plasmin [EC3.4.4.14] were examined. The inhibitoryeffects of various derivatives of L-phenylalanine and β-phenylpropionicacid on chymotryptic [EC3.4.4.5] activity were also examined.
No inhibitory effect on the caseinolytic and esterolytic activities oftrypsin was observed with 4-aminobutyl or 6-aminohexyl butyrate, andthese compounds were insensitive to trypsin.
Hexyl Nα-benzoyl-D-lysinate inhibited, though less than hexyl ε-aminocaproate, the caseinolytic and esterolytic activities of trypsin and plasmin, and was hydrolyzed slower by trypsin than the latter.
β-Phenylpropionic acid, β-phenylpropionamide and carbobenzo xyglycine strongly inhibited the esterolytic activity of chymotrypsin, but had little effect on caseinolysis. On the other hand, carbobenzoxy-L-phenylalanine strongly inhibited both esterolysis and caseinolysis. The inhibitory effect of β-phenylpropionic acid on esterolysis was reduced in the presence of a protein such as casein and albumin. Therefore, β-phenylpropionic acid does not seem to be a competitive inhibitor which combines with the active site of chymotrypsin. However, the inhibitory effect of carbobenzoxy-L-phenylalanine was not reduced in the presenceof albumin.
2-Aminoethyl and 6-aminohexyl β-phenylpropionate extensively inhibitedboth the caseinolytic and esterolytic activities of chymotrypsin, the inhibitory effects of the latter being the stronger.
The optical rotation of chymotrypsin was measured between 300 and 500mμ at 20°C in the presence and absence of 6-aminohexyl β-phenylpropionate.No difference between the two curves was observed.
These results are discussed on the basis of the “three-point theory”.抄録全体を表示PDF形式でダウンロード (2230K) -
I. The Reaction of Diisocyanates with Lysine and Enzyme ProteinsHITOSHI OZAWA1967 年 62 巻 4 号 p. 419-423
発行日: 1967/10/25
公開日: 2011/01/25
ジャーナル フリー1. N6, N6'-Hexamethylenebiscarbamoyllysine was synthesized by treating the copper complex of lysine with hexamethylenediisocyanate. The properties of this compound were described.
2. Ribonuclease [EC 2.7.7.16] was treated with hexamethylenediisocyanate.The derivative, in which 1.8 moles of the lysine residuesreacted with hexamethylenediisocyanate, had the full activity. The fullyactive derivative had simillar intrinsic viscosity and sedimentationcoefficient to that of ribonuclease. It seemed therefore to have oneintra-molecular bridge of hexamethylenediisocyanate.
3. Chymotrypsin [EC 3.4.4.5] treated with hexamethylenediisocyanatewas obtained as an insoluble and enzymatically active derivative. The activity of the insoluble fraction was about 30%of the original.抄録全体を表示PDF形式でダウンロード (2106K) -
II. Inhibition of RNA Polymerase ReactionYOSHITO KAZIRO, MIKIO KAMIYAMA1967 年 62 巻 4 号 p. 424-429
発行日: 1967/10/25
公開日: 2011/01/25
ジャーナル フリーChromomycin A3 , an antibiotic and antitumor substance producedby Streptomyces griseus No.7 (ATCC 13273), was shown to be a potentinhibitor of RNA polymerase [EC 2.7.7.6] reaction. About 50% inhibitionwas achieved in the presence of 0.1μg. of chromomycin A3 in the 0.25ml. of the reaction mixture containing 28μg. of native calf thymusDNA. The inhibition was reduced by prior heat denaturation of thetemplate DNA. The antibiotic did not inhibit the poly A synthesisprimed by denatured DNA, or the formation of AU copolymer withdeoxyAT copolymer as template.Thus, the mechanism of action ofchromomycin A3 is similar to that of actinomycin D.
From the studies with various derivatives of chromomycin A3, itwas concluded that the presence of sugar moiety is necessary for its effect as inhibitor of RNA synthesis.抄録全体を表示PDF形式でダウンロード (926K) -
TATSUO ISHIHARA, MASACHIKA IRIE, TYUNOSIN UKITA1967 年 62 巻 4 号 p. 430-438
発行日: 1967/10/25
公開日: 2011/01/25
ジャーナル フリー1. Two ribonucleases [EC 2.7.7.16], RNase Eq1 and Eq2, werepurified 180 and 130 fold, respectively, by successive IRC-50 and phosphocellulose column chromatography after ammonium sulfate fractionation of horse pancreas extract.
2. RNases Eq1 and Eq2 were found to be different from RNase Ain respect to the elution position on IRC-50 column chromatography.
3. RNase Eq1 was homogeneous in both sedimentational and electrophoreticanalyses, whereas RNase Eq2 was found to be still heterogeneousin electrophoretic examination.
4. RNases Eq1 and Eq2 have properties very similar to those ofRNase A in respect to molecular weight and heat stability.
5. RNases Eq1 and Eq2 have the base specificity similar to thatof RNase A and are markedly inhibited by Cu++, Zn++ and Hg++.抄録全体を表示PDF形式でダウンロード (2655K) -
I. Formation of Maltotriose in the Course of HydrolysisHozumi YOSHIDA, KEITARO HIROMI, SÔZABURO ONO1967 年 62 巻 4 号 p. 439-446
発行日: 1967/10/25
公開日: 2011/01/25
ジャーナル フリー1.The hydrolytic reaction of phenyl α-maltoside catalyzed by crystalline saccharifying α-amylase [EC 3.2.1.1] of B.subtilis was studied at 25°C and pH 5.4 by the measurements of total reducing value and the amounts of phenol produced and also by thin-layer chromatography.
2.The hydrolytic reaction was found to involve two parallel processes first, the hydrolysis between phenol and glucose residues to produce phenol and maltose, and the second, the hydrolysis between two glucose residues to produce glucose and phenyl α-glucoside.At lower substrate concentration, the first process is predominant, while the second becomes evident at higher substrate concentrations.
3.At higher substrate concentrations, the intermedial production of maltotriose was shown by thin-layer and paper chromatography.The maltotriose was also identified by optical rotatory dispersion, rates of hydrolyses by gluc-amylase and Taka-amylase A.A possibility of transglucosidation of this enzyme was suggested.抄録全体を表示PDF形式でダウンロード (7613K) -
MITSUO SUZUKI, KATSUYUKI IMAI, AKIO ITO, TSUNEO OMURA, RYO SATO1967 年 62 巻 4 号 p. 447-455
発行日: 1967/10/25
公開日: 2011/01/25
ジャーナル フリー1.Effects of thyroidectomy and injection of a physiological dose of triiodothyronine to the thyroidectomized animal on the levels of various oxidative enzymes in rat liver were studied.
2.All the microsomal activities concerned with NADPH oxidation were markedly decreased after thyroidectomy and elevated rapidly following the hormone administration.
3. Microsomal hemoproteins, cytochrome b5 and P-450, and microsomal NADH oxidizing activities remained unchanged for 10 to 15 days after thyroidectomy, but increased abruptly thereafter.The elevated levels could be reduced by the hormone injection.
4.Alcohol dehydrogenase [EC 1.1.1.1] of the soluble fraction showed similar, though not identical, responses to the microsomal hemoproteins.
5. The contents of cytochromes c+c1 and, to a lesser extent, of cytochrome a showed a tendency of gradual decrease after thyroidectomy, and returned to the normal levels on the hormone injection.
6. Soluble NADH diaphorase and mitochondrial cytochrome b were not affected by thyroidectomy and triiodothyronine administration.抄録全体を表示PDF形式でダウンロード (1517K) -
VII. Effect of Trinitrophenylation of Myosin on the Decomposition of Phosphoryl MyosinHARUHIKO TOKUYAMA, YUJI TONOMURA1967 年 62 巻 4 号 p. 456-463
発行日: 1967/10/25
公開日: 2011/01/25
ジャーナル フリーIt was already found that the ATPase [EC 3.6.1.3] activity of myosin in the presence of Mg++ is markedly enhanced by trinitrophenylation of myosin.Thereafter, the reaction mechanism of myosin-ATPase has been elucidated, and it was shown that myosin is a doubleheaded enzyme which hydrolyzes ATP by two different routes: one is simple hydrolysis of the Michaelis complex (ES), and the other is hydrolysis of ATP via phosphoryl myosin (EP).
The purpose of the present paper is to report studies of the kinetic mechanism of enhancement of Mg++-ATPase activity in steady state by trinitrophenylation of myosin, on the basis of the reaction mechanism mentioned above.We obtained the following results.
1. In the presence of 1 M KCl and 20mM MgCl2, the rate of ATPase of trinitrophenyl (TNP)-myosin in steady state was much higher than that of the control myosin, and P1 was continuously liberated from the TNP-myosin-ATP system at a high rate, about equal to that of the initial burst of Pi-liberation observed on the control myosin.
2. Both the ATPase activity of actomyosin type and the superprecipitation of actomyosin by ATP were unaffected by trinitrophenylation of myosin.
3.The rate of ATPase of the control myosin in steady state was independent of the size of the initial burst, but the rate of ATPase of TNP-myosin decreased when the size of initial burst was reduced by p-nitrothiophenylation or prolonged incubation of myosin.The rate of ATPase of TNP-myosin reached the level of control myosin, when the size of the initial burst became zero.From these results, it was concluded that, at least in the presence of Mg++ and a high concentration of KCl, trinitrophenylation of myosin has no significant effect on the simple hydrolysis of ES, but accelerates markedly the decomposition of EP.抄録全体を表示PDF形式でダウンロード (1393K) -
YOSHIO IMAI, RYO SATO1967 年 62 巻 4 号 p. 464-473
発行日: 1967/10/25
公開日: 2011/01/25
ジャーナル フリーThe unusual difference spectrum appearing on combination of ethyl isocyanide with reduced P-450 in dithionite-treated liver microsomes was profoundly influenced by pH. Of the two Soret bands in the spectrum, the peak at 45 mμ was intensified progressively as pH was increased, and this was accompanied by a corresponding decrease in the intensity of another peak at 430mμ. Both peaks were competitively affected by CO to the same extent at any pH value and ethyl isocyanide concentration tested.The intensities of both peaks were similarly dependent on the ethyl isocyanide concentration, and the apparent dissociation constant determined from this dependence was 4.1μM regardless of the pH employed.
Interactions of aniline and pyridine with reduced P-450 also brought about spectral changes having two Soret bands.These spectra were also affected by pH in a similar way to that observed in the ethyl isocyanide interaction.
It was suggested that reduced P-450, when in combination with lipophilic ligands such as ethyl isocyanide, aniline and pyridine, exists in two interconvertible states which are in a pH-dependent equilibrium.
On combination with ethyl isocyanide, reduced P-420, the modified product of P-450, gave rise to only one Soret band, which was not affected by pH.The effect of the ethyl isocyanide concentration on this Soret band was, however, somewhat unusual.抄録全体を表示PDF形式でダウンロード (1802K) -
I. Subunit Structure of Wheat Fraction-I ProteinTATSUO SUGIYAMA, TAKASHI AKAZAWA1967 年 62 巻 4 号 p. 474-482
発行日: 1967/10/25
公開日: 2011/01/25
ジャーナル フリーFraction-I protein, isolated from wheat leaves, was purified by Sephadex gel filtration and DEAE cellulose column chromatography. Its component subunits were studied after cleavage with SDS. Parallel to the disruption of the protein into subunits, as demonstrated by polyacrylamide gel electrophoresis, a proportionate decline in ribulosediphosphate carboxylase [EC 4.1.1.39] activities was observed. The structural and functional role of SH-groups in the protein molecule was studied by the reaction with PCMB. Mercaptide formation was paralleled by loss of carboxylase activity. Preincubation of the enzyme with RuDP prevented the inhibitory action of PCMB, indicating possible participation of the SH-group in the active site. Extent of mercaptide formation as determined by spectrophotometry was the same for enzyme with and without RuDP preincubation. Total SHgroups per protein molecule (M.W.5.15×105) were 96. Thus only a minor fraction of the SH-groups in the protein may take part in the enzymatic reaction. Polyacrylamide gel electrophoresis provides evidence for conformational changes of the protein subunit structure by PCMB treatment. The protein molecule appears to cleave into large and small fragments, accompanied by a concomitant loss of the enzyme activity. Addition of cysteine caused the restoration of enzyme activity and reformation of a protein band indistinguishable from that of original fraction-I protein.抄録全体を表示PDF形式でダウンロード (4604K) -
JUDITH THOMSON, DAVID B. MORELL1967 年 62 巻 4 号 p. 483-486
発行日: 1967/10/25
公開日: 2011/01/25
ジャーナル フリー1. The spectroscopic properties of oxidized and reduced salivary gland peroxidase [EC 1.11.1.7] in the visible region are shown to be similar to those of the same forms of milk peroxidase and different from those of horse-radish peroxidase. Like milk peroxidase haematin the prosthetic group of salivary gland peroxidase is shown to have a conjugated, electrophilic, alkali-labile substituent and to be covalently bound to the enzyme protein.
2. The enzyme is shown to be soluble after centrifugation of gland homogenates at 200, 000×g for 1 hour.
3. The distribution of the enzyme in the various salivary glands of mammals of differing species is examined.抄録全体を表示PDF形式でダウンロード (756K) -
TAKASHI MURAMATSU1967 年 62 巻 4 号 p. 487-491
発行日: 1967/10/25
公開日: 2011/01/25
ジャーナル フリーα-Mannosidase [EC 3.2.1.24] was purified 90-fold from the liver of Charonia lampas, using p-nitrophenyl α-D-mannoside as substrate. The enzymological properties of the purified enzyme are described. The purified a-mannosidase was practically free from other various glycosidases tested and it produced mannose from ovalbumin glycopeptide. A mixture of partially purified glycosidases was obtained from the liver of the gastropod, using Sephadex G-200 column chromatography.抄録全体を表示PDF形式でダウンロード (845K) -
HIROYUKI KOIKE, TADAO WADA, HARUHIKO MINAKAMI1967 年 62 巻 4 号 p. 492-494
発行日: 1967/10/25
公開日: 2011/01/25
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TAKAKO MASUI, KAZUO AMIMOTO, TARO KAZUNO1967 年 62 巻 4 号 p. 495-496
発行日: 1967/10/25
公開日: 2011/01/25
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AKIRA OHASHI, NOBUO ISHIHARA, GORO KIKUCHI1967 年 62 巻 4 号 p. 497-500
発行日: 1967/10/25
公開日: 2011/01/25
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OSAMU TAKENAKA, HIROO HORINISHI, KAZUO SHIBATA1967 年 62 巻 4 号 p. 501-503
発行日: 1967/10/25
公開日: 2011/01/25
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MITSUO YANO, SHIGEHARU NAGASAWA, KUNISUKE HORIUCHI, TOMOJI SUZUKI1967 年 62 巻 4 号 p. 504-506
発行日: 1967/10/25
公開日: 2011/01/25
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KIHACHIRO UEHARA, TADASHI MIZOGUCHI, SABURO HOSOMI1967 年 62 巻 4 号 p. 507-508
発行日: 1967/10/25
公開日: 2011/01/25
ジャーナル フリーPDF形式でダウンロード (284K)
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