1. Studies were made to find the favorable temperatures and concentrations of colchicine solution to induce amphidiploid in germinating seeds of Triticum vulgare × Secale cereale F
1. The concentrations tested were 0.025 % and 0.05 %, and the temperatures were high (25°C), medium (11∼17°C) and low (4∼6°C. The treatments were carried for 24 hrs. 2. The percentage of swelled plants was high at low temperatures, being Ca. 40 in 0.025 % solution and Ca. 47 in 0.05 %, while it was Ca. 7∼29 in the other plots. The percentage of plants which died was high in the 0.05 % solution of high and medium temperatures, being Ca. 92 and 75 respectively, while, it was Ca. 44∼57 in other plots. The plants other than those above mentioned grew normally. 3. By the size of stomata in young plants, the chromosome doubling was tentatively judged. The percentage of plants in which the chromosomes have not been doubled was 0∼20, corresponding nearly to that of normal plants judged by external appearance. The percentage of chimera as was high in 0.025 % solution at medium and low temperatures, being Ca. 21 and 26 respectively, while it was Ca. 4∼15 in other plots. The percentage of plants classified as those in which the chromosome doubling was widely occurred was the highest, Ca. 34, in the 0.05 % solution of low temperature while it was Ca. 3∼17 in the other plots. 4. By the fertility of matured plants, the chromosomal doubing was checked. The number of plants which set grains was found smaller than that of chimeras and the plants in which the doubling was assumed to have widely occurred from the stomatal size. Most of the plants setting grains, however, were found among those that were classified as chimeras and the plants in which the doubling was widely occurred. The percentage of plants setting grains was the poorest, Ca. 3, in 0.05 % solutions of high temperature, while at low temperatures it was Ca. 12 in 0.025 % and Ca. 13 in 0.05 % solutions. 5. So far as the present experiment is concerned, the effect of colchicine on the germinating seeds of Triticum vulgare × Secale cereale F
1 was the best at low temperature, the concentrations of solution of both 0.05 % and 0.025 % showing almost the same result.
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