To investigate the composition of photosynthetic products translocated from the leaf of flue-cured tobacco plant,
14CO
2 was supplied to a part of mesophyll, and the distribution of
14C among various compounds in the veins of 1st order of branching and the midrib was traced during the period of 180 minutes after
14CO
2 feeding. Results obtained were summarized as follows: 1) In the veins of 1st order of branching and the midrib, about 70% of the total
14C was found in sucrose, and a little amount of
14C in glucose and fructose. Therefore, it was concluded that sucrose was the main transport substance in flue-cured tobacco plant. 2) The assay of
14C-components of cationic fraction in the veins of 1st order of branching and the midrib showed that most of
14C in fraction was incorporated into serine at the time of 30 and 60 minutes after
14CO
2 feeding, thereafter,
14C-serine decreased. On the contrary, the radioactivities in aspartic acid, glutamic acid, glutamine, and γ-aminobutyric acid increased with time. 3) Radioactive malic acid was found in the mesophyll and the veins of 1st order of branching, but not in the midrib, 4) In the veins of 1st order of branching, the incorporatibn of
14C into the insoluble fraction and the hydrolysis of the translocated
14C-sucrose were found to be more profound than those in the midrib. From these facts, it was inferred that the
14C translocated into the veins of 1st order of branching was more actively utilized for formation of the tissue, comparing with the
14C in the midrib.
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