日本食品微生物学会雑誌
Online ISSN : 1882-5982
Print ISSN : 1340-8267
ISSN-L : 1340-8267
31 巻 , 1 号
選択された号の論文の5件中1~5を表示しています
総説
教育講演
原著
  • 川崎 晋, 清水 茂雅, 小関 成樹, 稲津 康弘
    原稿種別: 原著
    2014 年 31 巻 1 号 p. 28-35
    発行日: 2014/03/31
    公開日: 2014/08/13
    ジャーナル フリー
    The Salmonella growth was monitored in pasteurized and non- pasteurized milk using real-time PCR, and growth prediction model was constructed based on the results. Pasteurized and non- pasteurized milk were inoculated with Salmonella to a concentration of 104 CFU/ml, and the Salmonella growth was monitored for 168 hours at 4℃ to 35℃ using traditional culture method and real-time PCR. With real-time PCR, total-DNA was directly extracted from 1 ml of pasteurized and non- pasteurized milk, and the copy number of Salmonella invA was quantified and converted to the Salmonella cell count. The Salmonella cell counts estimated by traditional culture method and real-time PCR in pasteurized milk were very similar, and these results were reflecting the MRV predictive model. A predictive growth curve was constructed from the Salmonella cell count estimated using real-time PCR, and a relational expression for the maximum growth rate (μmax) and incubation temperature was derived using the square-root model. Comparison of μmax between pasteurized and non-pasteurized milk showed a slightly lower μmax of Salmonella for non- pasteurized milk compared to pasteurized milk, and the maximum cell concentration also tended to be lower for non- pasteurized milk. Application of the real-time PCR monitoring and predictive microbiological analysis enables rapid provision of a growth prediction formula that includes the effects of microbacterial flora and food matrices.
  • 村田 敏夫, 柳生 裕子, 三瓶 美香, 青木 敏也
    原稿種別: 原著
    2014 年 31 巻 1 号 p. 36-40
    発行日: 2014/03/31
    公開日: 2014/08/13
    ジャーナル フリー
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