日本食品微生物学会雑誌
Online ISSN : 1882-5982
Print ISSN : 1340-8267
ISSN-L : 1340-8267
25 巻 , 2 号
選択された号の論文の8件中1~8を表示しています
シンポジウム:食品と有害カビ ―今何故カビが注目されるか?―
原著
  • 門間 千枝, 下島 優香子, 小西 典子, 尾畑 浩魅, 石崎 直人, 仲真 晶子, 甲斐 明美, 柳川 義勢, 山田 澄夫
    原稿種別: 原著
    2008 年 25 巻 2 号 p. 76-82
    発行日: 2008/06/30
    公開日: 2008/08/01
    ジャーナル フリー
    A total of 94 patients who had eaten identical meals suffered from diarrhea and abdominal pain in April 2006. Clostridium perfringens was suspected as the causative pathogen based on epidemiological investigation. Bacteriological examination of 60 fecal specimens of the patients using Clostridium welchii (CW) agar with kanamycin (0.2 g/l) and egg yolk for the conventional isolation method identified strains of C. perfringens serotype Hobbs 17 and Hobbs 13 from 49 (81.7%) and 22 (36.7%) of the specimens, respectively. Despite the high isolation rate of these organisms, isolates did not produce C. perfringens enterotoxin. Further examination using CW agar without kanamycin resulted in the isolation of enterotoxin-producing C. pserfringens serotypes TW 1 and TW24 from 31 (51.7%) and 26 (43.3%) fecal specimens, respectively. Both serotypes were isolated from a total of 4 fecal specimens.
    Although almost all C. perfringens strains are resistant to kanamycin (minimum inhibitory concentration, MIC>256 μg/ml), isolates of serotypes TW 1 and TW24 had MIC for kanamycin of 64-128 μg/ml. Therefore, the number of those that grew onto CW agar without kanamycin were about 1,000∼10,000 times more than onto CW agar with kanamycin.
  • 中野 千紗, 清水 晃, 河野 潤一, 北井 智, 北川 浩
    原稿種別: 原著
    2008 年 25 巻 2 号 p. 83-88
    発行日: 2008/06/30
    公開日: 2008/08/01
    ジャーナル フリー
    Nasal samples of humans and animals were examined for the presence of Staphylococcus aureus, and subsequently the incidence of enterotoxigenic and methicillin-resistant S. aureus. Additionally, characteristics of the isolates were studied. The carrier rate of S. aureus was 42.7% (38/89) for humans, 86.4% (57/66) for pigs, 19.8% (20/101) for cows, and 9.5%(4/42) for chickens. By using a commercial SET-RPLA kit, 22 isolates from humans produced staphylococcal enterotoxin (SE) A (n=8), SEB (n=9), and SEC (n=5). S. aureus isolates were subjected to genotyping analysis for detection of se genes (sea to see, seg, seh and sei). The se genes were detected in 25 (65.8%) of 38 human, 34 (59.6%) of 57 pig, and 4 (100%) of 4 chicken isolates. Twenty-five human isolates possessed the sea (n=8), seb (n=4), seb-seg-sei (n=5), sec-sei (n=4), sec-seg-sei (n=1) or seg-sei (n=3) genes. Thirty-four isolates from pigs and 4 isolates from chickens possessed seg-sei genes.
    MRSA was detected from only 5 food handlers. Phenotypic and genotypic characteristics of 3 MRSA isolates from the 3 food handlers in the same facility were identical except for two phenotypic characteristics. Therefore, the results may suggest that the horizontal transmission of MRSA occurred in the facility.
  • 大仲 賢二, 古畑 勝則, 原 元宣, 福山 正文
    原稿種別: 原著
    2008 年 25 巻 2 号 p. 89-97
    発行日: 2008/06/30
    公開日: 2008/08/01
    ジャーナル フリー
    Vibrio vulnificus 感染症に関する基礎的研究の一環として感染経路や感染源を解明するため,今回は市販海産魚類の本菌の汚染状況,血清型別および各種抗菌剤の薬剤感受性試験を行ったところ,以下の成績が得られた.
    1. 新鮮魚1049例について本菌の分離を試みたところ,57例(5.4%)から本菌が分離された.
    2. 産地別における分離状況は,鳥取県が157例中1例(0.6%),徳島県が154例中21例(13.6%),愛媛県が90例中26例(28.9%),神奈川県が443例中9例(2.0%)からそれぞれ本菌が分離され,徳島県と愛媛県が高い分離率を示し,産地間の差異が認められた.
    3. 魚種別では,アジ613例中53例(8.6%),イワシ191例中4例(2.1%)およびカマス27例中1例(3.7%)からそれぞれ本菌が分離された.
    4. 検査部位別では内蔵14例(1.3%),エラ18例(1.7%)および体表26例(2.5%)からそれぞれ本菌が分離された.
    5. 血清型別状況では,供試した58株中32株(55.2%)が9菌型に型別され,O22が19.0% と最も多く,次にO4が10.3% などであった.
    6. 薬剤感受性試験結果をMIC90値で比較すると,GM, EM, TC, DOXY, MINO, CP, NAおよびCPFXに対して全株が感受性を示したが,ABPC, PIPC, CER, CET, CPZ, CTX, CMZ, LMOX, MEPM, CTX, KMA, MKおよびLCMに対しては耐性株が認められた.
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