Kanzo
Online ISSN : 1881-3593
Print ISSN : 0451-4203
ISSN-L : 0451-4203
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Volume 25, Issue 4
Displaying 1-19 of 19 articles from this issue
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  • Hepatitis B virus infection and integration of viral genome in hepatocellular carcinomas of the Japanese
    Okio HINO, Tomoyuki KITAGAWA, Haruo SUGANO, Wataru MORI, Toshiro NAKAS ...
    1984 Volume 25 Issue 4 Pages 463-469
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.463
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    State of hepatitis B virus (HBV) DNA in hepatocellular carcinomas (HCC) of Japanese patients in Japan were investigated by molecular hybridization method utilizing a 32P-labeled cloned HBV DNA of adr subtypes as a probe.
    Among 51 HCC cases analysed, integrated HBV DNA was detected in 13 out of 14 (93%) HBsAg positive cases, 4 out of 8 (50%) HBV Ab positive cases (HBsAb and/or HBcAb and/or HBeAb) and 7 out of 29 (24%) clinically "nonA nonB" cases.
    The restriction pattern of integrated viral DNA was different from one to another. There was no remarkable histological difference between HBV DNA positive and negative HCC. In the noncancerous portion of HCC, free and random integration was demonstrated.
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  • Demonstration of IgA class anti-HBc in chronic HBsAg carriers
    Motozumi NOMURA, Seiichi FRUTA, Yoshihiro AKAHANE, Katumi TACHIBANA, S ...
    1984 Volume 25 Issue 4 Pages 470-476
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.470
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    The assay method for IgA class anti-HBc was developed, which was solid-phase radioimmunoassay using microtiter plates coated with anti-α-chain monoclonal antibody and radiolabelled anti-HBc monoclonal antibody. IgA class anti-HBc was tested by this method in 187 sera from chronic HBsAg carrier.
    In two (4.2%) of 47 asymptomatic carriers IgA class anti-HBc was detected. On the other hand, in 109 (77.8%) of 140 HBsAg-positive patients with chronic liver disease IgA class antiHBc was detected and in addition the titers in these case were significantly higher than those in asymptomatic carrier. The titers in each CAH, LC, HCC were significantly higher than those in CIH.
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  • Serological differentiation between patients with acute hepatitis B and chronic HBs-Ag carriers
    Yoshihiro AKAHANE, Kendo KIYOSAWA, Motozumi NOMURA, Shuichi WADA, Take ...
    1984 Volume 25 Issue 4 Pages 477-482
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.477
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    In an attempt to differentiate acute HBV infection from persistent HBs-Ag carrier state serologically on single serum sample, IgM class and IgG class antibodies to hepatitis B core antigen were tested.
    IgM anti-HBc titer was significantly higher in acute hepatitis B (42 cases) than in chronic HBs-Ag carriers (183 cases). The titer of this class antibody, however, was relatively low in 2 cases with acute hepatitis B and moderately high in 15 cases with HBs-Ag positive chronic liver diseases, leading to the difficulty in making a differentiation between two categories of HBV infection by the assay of IgM class anti-HBc alone.
    Hence, the ratio of IgM class anti-HBc to IgG class anti-HBc was calculated. This ratio was over 0.9 in 41 of 42 cases with acute hepatitis B and below 0.9 in 180 of 183 cases with chronic HBs-Ag carriers. Thus, the determination of this ratio was found to be of great value in separating acute HBV infection from chronic HBs-Ag carrier state.
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  • Quantative measurement of hepatitis B e antigen by enzyme immunoassay
    Kiyohiko KURAI, Shiro IINO, Kazuhiko KOIKE, Hiroshi SUZUKI, Keiji MITA ...
    1984 Volume 25 Issue 4 Pages 483-490
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.483
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    We made an attempt at quantative measurement of hepatitis B e antigen (HBeAg) by enzyme immunoassay (EIA).
    Its measurable range was from one to 100 units. (This is tentatively established unit.) In the specimen contained high concentration of HBeAg over 100 units, its concentration is determined after appropriate dilution.
    At reproducibility test, the coefficient of variation in intra- and inter-assays were 4.24-11.82%, 8.57-13.39%, respectively and the mean recovery was 98.45% (range 82.6% to 109.1%).
    In respect of the relationship between concentration and cutoff index of HBeAg in EIA method, they were correlated with together in the low concentration range, but its relationship dissociated when cutoff index exceeded 2.5.
    Concentration of HBeAg were not correlated with hepatitis B virus (HBV)-associated DNA polymerase activities and hepatitis B surface antigen (HBsAg) titers in the cases of HBsAgpositive chronic hepatitis.
    This assay system is very useful to put forward the clinical approach to HBV infection.
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  • A histopathological study of alcoholic liver fibrosis, especially on characteristic changes of perisinusoidal reticulin fibers
    Masahiro AIHARA
    1984 Volume 25 Issue 4 Pages 491-500
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.491
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    The author have intended to clarify the morphological characteristics of alcoholic hepatic fibrosis, especially those changes which occur on perisinusoidal reticulin fibers. Biopsy specimens were obtained from 95 cases of chronic alcoholics, 67 cases with chronic hepatitis type B, and 30 individuals showing minimal changes.
    The results obtained were as follows: 1) In normal livers, perisinusoidal reticulin fibers were distinguished into four categories, i.e., thick, intermediate, thin and fine reticulin fibers. The principal characteristics of each fibers and their density per unit sinusoids were described in detail, respectively. 2) In alcoholic liver injuries, increased numbers of stretched intermediate and fine reticulin fibers were recognized as a characteristic finding. It was disclosed most prominently in the circulatory periphery of the hepatic lobuli. An increase of fine reticulin fibers was found frequently even in cases with a little amount of total ethanol intake. 3) The distorted pattern of perisinusoidal reticulin fibers in chronic hepatitis was different from those in alcoholic liver injuries.
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  • Histopathological and histometrical studies on drug-induced acute hepatic injuries
    Makoto OHBU
    1984 Volume 25 Issue 4 Pages 501-513
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.501
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    Histopathological and histometrical studies on drug-induced acute hepatic injuries were made. Liver biopsy specimens submitted were as follows: Forty-two cases with acute drug-induced hepatic injury to hypersensitivity reaction (DIH), 52 cases with acute hepatitis type B (HB), 19 cases with obstructive jaundice and 22 cases with minimal changes as a normal control. Results obtained were as follows: 1. Multinucleated hepatocytes containing more than three nuclei were detected more abundantly in DIH than in HB and obstructive jaundice. Intralobular distribution of the cells was predominantly centrilobular. 2. Bile canalicular bile plugs were found to be more prevalent per unit area in DIH than in HB, and in the former, bile plugs were observed for long periods after the clinical onset. In HB, they showed a remarkable tendency to disappear in a relatively early stage. 3. In DIH, the amount of the multinucleated hepatocytes was closely correlated to that of bile plugs. No such relationship was found in HB. 4. Cholestasis in DIH was predominantly centrilobular and the pattern was clearly different from that in obstructive jaundice. It was concluded that the above findings would be useful as practical "hallmarks" for histopathological diagnosis on drug-induced acute hepatic injury.
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  • Immunological studies on primary biliary cirrhosis
    Kozo MIZUIRI, Kosho CHIN, Kayoko TASHIRO, Katsushiro MO, Masayuki MIZU ...
    1984 Volume 25 Issue 4 Pages 514-522
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.514
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    PHA and Con A responses, autologous mixed lymphocyte reaction (AMLR) and prostaglandin producing suppressor function (PPSF) in 6 cases of primary biliary cirrhosis (PBC), and the effect of corticosteroid on peripheral mononuclear cells and immune responses were studied in healthy volunteers. Although mitogen responses (S.I.) and PPSF were not abnormal, AMLR was significantly reduced in PBC. When methylprednisolone pulse therapy was tried in a case of PBC, clinical and laboratory findings and AMLR were improved and serum inhibitory factors were disappeared.
    AMLR and Con A response were reduced and PPSF increased after oral administration of 30mg of prednisolone to normal controls.
    These results suggest that the immunoregulatory function is impaired and methylprednisolone pulse therapy seems to improve clinical findings and abnormal immune responses in some of PBC. Corticosteroid seems to have some influence on PPSF.
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  • So-called idiopathic portal hypertension with alteration of hepatic venous system
    Atsuko ARAI, Toshio SHIKATA
    1984 Volume 25 Issue 4 Pages 523-531
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.523
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    Characteristic liver pathology of Banti's disease or idiopathic portal hypertension is fibrosis of Glisson's capsules and stenosis of portal veins. In order to establish the origin of the disease, it is essential to study pathology of the center of lobulus, central veins and hepatic veins.
    It is important to consider about the findings whether it is primary or secondary change. There are old fibrosis in the Glisson's capsules and the center of lobulus. It proves that the pathological process injured Glission's capsules and the liver parenchy, a at the same time. Remarkable findings were unusually dilated central and hepatic veins even though hepatic blood flow supposed to be decreased. It is thought that these veins connect to the abnormal blood vessels around the Glisson's capsules and forming intrahepatic bypass.
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  • Establishment and charcterization of human hepatoma cell line (KIM-1)
    Tatsuo MURAKAMI
    1984 Volume 25 Issue 4 Pages 532-539
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.532
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    Hepatocellular carcinoma (HCC) cell line, KIM-1, was established from the surgically removed tumor of a α-fetoprotein (AFP) seropositive and HBs-Ag seronegative hepatoma patient. The KIM-1 cells not only resembled the original HCC tissue on light and electron microscopic examinations, but also produced some tumor markers such as AFP, β2-microglobulin (BMG) and ferritin. The cells grow both in serum-containing medium and in serum-free medium. When KIM- 1 cells were injected into athymic nude mice subcutaneously, they introduced tumor which resembled the original HCC tissue histologically, and the serum AFP level of the mice were extremely high. The range in chromosome number laid between 71 and 160 with some marker chromosomes, and the modal chromosome number was 79. This cell line will provide a good experimental model for venous investigations.
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  • Hepatoma, livercirrhosis and serum alpha-1 micro globulin
    effects of hepatectomy
    Toshihiko TSUBURAYA, Toru TAMAKI, Yoshinobu AKASAKA, Michiaki MATSUSHI ...
    1984 Volume 25 Issue 4 Pages 540-548
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.540
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    The function of Alpha-1 microglobulin (A-1m), a low molecular serum protein, is still unknown, but it is generally found to be produced by normal hepatocytes and hepatoma cells.
    In this study, serum A-1m level is estimated by using enzyme-immunoassay in patients with various liver diseases and in hepatectomized patients.
    Serum A-1m level correlate significantly with that of hepaplastin test, serum antithrombin three and serum choline esterase (p<0.001).
    The serum level of A-1m in normal healthy adults ranges from 8.0 to 14.0mg/l and slightly increased in aged group.
    The level of A-1m of the patients with cirrhosis is lower than that of normal adults significantly. On the contrally the hepatoma group dose not show any significalt difference whether with cirrhosis or not.
    After hepatic resection, the concentration of A-1m decreased rapidly corresponding to the volume of remained liver and the severity of coexisting cirrhosis. And in hepatocellular carcinoma without cirrhosis, rapid recovery to normal range was observed.
    We concluded that the determination of A-1m in serum is good indicator of functional recovery of the remained liver after hepatectomy.
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  • Pathomorphological study on primary liver cancer
    a clinicopathologic study of cholangiocarcinoma
    Naoto TOKUNAGA
    1984 Volume 25 Issue 4 Pages 549-556
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.549
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    Among 3203 autopsies in adult at Pathology Department of Kurume University Hospital during the 12 years from 1971 to 1982, cholangiocarcinoma was seen in 45 cases (1.4%). The 45 cases of cholangiocarcinoma were classified into two types, peripheral type (10 cases) and hilar type (35 cases).
    Age ranged from 41 to 81 years old and the average was 63.0±11.4 years old. Male to Female ratio was 28:17.
    Main clinical symptoms were right chest pain, abdominal pain, and general malaise in the peripheral type, and were jaundice and general malaise in the hilar type. Jaundice was seen in 94.3% of the hilar type during the course, but was 40.0% of the peripherl type. Furthermore, jaundice was not seen in the peripheral type as an initial symptoms, but it was seen 40.0% of the hilar type.
    Mixed macro-and micronodular cirrhosis and biliary cirrhosis associated in 4 cases, respectively.
    Stones of the intra-and extrahepatic bile ducts were associated in 6 cases among the 10 cases of the peripheral type, and in 7 cases among the 35 cases of the hilar type.
    Histologically, most of the cases were well-differentiated adenocarcinoma and were accompanied by abundant fibrous stroma. Carcinoembryonic antigen was demonstrated in all the cases by immunoperoxidase technique. Metastases were seen in 35 out of 45 cases (77.8%) and there was no difference as to frequency and ratio of metastasis between the peripheral type and hilar type.
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  • The liver change in male hypogonadism
    Hiroshi NAKANO, Shuji SEKO, Katsuji KOHIGASHI, Tsunehisa KAWASAKI, [in ...
    1984 Volume 25 Issue 4 Pages 557-560
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.557
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    Elevated levels of serum transaminases, lactic dehydrogenase and choline esterase were observed in 5 of 10 patients with male hypogonadism. All these five patients showed hyperlipedemia. Two of 5 patients with abnormal blood enzymatic changes were hypogonadotropic and remainders were hypergonadotropic hypogonadism.
    Liver biopsy was performed in one of these five patients and revealed the presence of marked fatty infiltration in the liver.
    Marked obesity (>+20%) was observed in 3 of 5 patients with abnormal liver function tests and 1 of 5 patients with normal liver function tests.
    These results suggest that disturbed lipids metabolism may cause the existence of fatty liver in male hypogonadism.
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  • A case of adenomatous hyperplasia of the liver detected by peritoneoscopy and real-time ultrasonography
    Shigeru MORICHIKA, Kazuo TOBE, Takahiro TSUCHIYA, Takayoshi TANABE, Ke ...
    1984 Volume 25 Issue 4 Pages 561-566
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.561
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    Adenomatous hyperplasia of the liver was detected in 62-year-old man with liver cirrhosis by peritoneoscopy and real-time ultrasonography (US). Peritoneoscopy revealed a reddish-brown, hemispherical tumor, 2.5cm in diameter, lateral to the gallbladder. Several small vessels were seen on the surface. Hepatocellular carcinoma was suspected. US demonstrated a well-demarcated hypoechoic lesion with internal hyper-echoic lines. Plain CT, contrast-enhancement CT, and scintigraphy did not reveal the lesion. The wedge-resected tumor was histologically diagnosed as an adenomatous hyperplastic growth consisting of two pseudolobules, 1cm in diameter each. the tumor contained portal triads, and the cell morphology did not differ from that in the surrounding cirrhotic liver. US and CT imaging of adenomatous hyperplasia have not been discussed previously. The case demonstrates the importance of differentiating adenomatous hyperplasia from hepatocellular carcinoma when a small, hypo-echoic lesion is detected by US in a cirrhotic liver.
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  • Hepatitis due to methoxyflurane
    Tadao OKUNO, Makoto TAKEDA, Masahide HORISHI, Masaharu OHTA, Yoshinori ...
    1984 Volume 25 Issue 4 Pages 567-572
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.567
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    A 39-year-old gynecologist was admitted to the hospital because of severe general malaise and jaundice. He had been repeatedly inhaled a small dose (about 2ml) of methoxyflurane almost every day for his insomnia by an infiltrated gauze placed over his mouth. He had consumed a total of about 125ml of methoxyflurane during one month and then he developed icteric hepatitis. Subsequently, he successfully recovered from hepatitis. On admission, the GOT was 165KU, the GPT 400KU, the ALP 23.8KAU and the total bilirubin 12.7mg/dl. There was no eosinophilia on the blood smear. The liver biopsy was done on the 18th day after the onset of illness. Light microscopically, there was a confluent liver cell necrosis in the centrilobular area. Electron microscopically, the conspicuous findings were many irregularly shaped fat like vacuoles, partially enveloped by a membrane and increased number of secondary lysosomes in the hepatocytes. The intercellular spaces were dilated with well developed microbilli.
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1984 Volume 25 Issue 4 Pages 573
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.573
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1984 Volume 25 Issue 4 Pages 574
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.574
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese]
    1984 Volume 25 Issue 4 Pages 575
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.575
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    1984 Volume 25 Issue 4 Pages 576
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.576
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  • [title in Japanese]
    1984 Volume 25 Issue 4 Pages 577-600
    Published: April 25, 1984
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.25.577
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