Kanzo
Online ISSN : 1881-3593
Print ISSN : 0451-4203
ISSN-L : 0451-4203
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Volume 26, Issue 5
Displaying 1-29 of 29 articles from this issue
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  • Localization of duck hepatitis B virus in the liver
    Toshikazu UCHIDA, Koyu SUZUKI, Mariko ESUMI, Masashi OOMURA, Tadashi I ...
    1985 Volume 26 Issue 5 Pages 559-564
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.559
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    Localization of DHBV was investigated in the liver of ducks utilizing immunoperoxidase and electron microscopic methods. Inoculation of DHBV-positive sera within 24 hours after hatching resulted in persistent infection as demonstrated by DHBV-DNA and DNA polymerase activity in the sera. The immunostaining showed staining of DHBV in the cytoplasm of hepatocytes andbiliary epithelial cells. Ultrathin sections of the pellet of DHBV-positive sera showed two kinds of virus particles. One was 34 to 63nm in diameter and the other (probably virions, 40nm in diameter) had a markedly electron-dense core with and envelope.
    The DHBV-positive livers had many envelope-like and a few virion-like particles within the cisternae of endoplasmic reticula and a few core-like particles in the hyaloplasm of hepatocytes. The localization in the hepatocytes seems to be somewhat similar between DHBV and HBV.
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  • Southern blot-hybridization analysis of HBV DNA in patients with HBsAg seropositive chronic liver diseases
    Osamu YOKOSUKA, Masao OMATA, Fumio IMAZEKI, Yoshimi ITO, Junko MORI, K ...
    1985 Volume 26 Issue 5 Pages 565-571
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.565
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    Liver tissues taken from 64 patients with HBsAg positive chronic liver disease (nonneoplastic) were analyzed by the method of Southern blot and HBV DNA hybridization. HBV DNA was detected in 36 of 38 (95%) HBeAg seropositive patients, in 3 of 7 (43%) HBeAg-antiHBeAb negative patients, and in 7 of 19 (37%) anti-HBeAb positive patients. Free HBV DNAs including single stranded, partially double stranded and supercoiled HBV DNA were found in almost all HBeAg positive patients and also in 19% of HBeAg negative or anti-HBeAb positive patients. These patients with "active" type or actively replicating HBV DNA despite HBeAg negativity tellded to show higher sGPT levels and advanced liver diseases. Free viral DNA with "inactive" type HBV DNA which had only supercoiled (no evidence of active replication) was found in 3 cases (1 with eAg-/eAb-, and 2 with eAg-/eAb+).
    Integrated HBV DNA was found in 4 cases. "Clonal" type integration which shows distinct high molecular bands after enzyme digestion was found in 2 cases, both with cirrhosis. "Random" type integration which shows only a smear after restriction enzyme digestion was found in 2 cases with chronic persistent hepatitis.
    Southern blot hybridization of hepatic HBV DNA could reveal not only integration of hepatic HBV DNA into genomic DNA, but also the state of various episomal replicative forms.
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  • Identification of HBV-associated antigens in nontumorous lesions of hepatomas by immunoperoxidase study
    In comparison with the antigens in tissues of CAH and LC
    Masaki IWAI, Hiroyuki SHINTANI, Takeshi OKANOUE, Tadao OKUNO, Tatsuro ...
    1985 Volume 26 Issue 5 Pages 572-577
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.572
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    It is well known that hepatocellular carcinoma (HCC) develops among liver disordors with chronic carrier of HBsAg. To aim at discussing the relation of hepatitis B virus (HBV) with evolutioll of HCC, We demonstrated HBsAg in chrOnic active hepatitis and liver cirrhosis with its carrier by immunoperoxidase study and investigated HBV-associated antigens in nontumorous lesions of HCC. HBsAg was predominantly detected on membrane of hepatocytes in chronic active hepatitis and in cytoplasm in liver cirrhosis. Distribution pattems of HBsAg in nontumorous lesions of HCC were not only festoon or cytoplasmic type but also inclusion body type. Membranous type is associated with active inflammation and inclusion body type is suggested to relate with retension of HBsAg due to disturbance of its excretion. HBcAg were distributed scatteredly, diffusely and in a cluster in each pseudolobule of liver cirrhosis with hepatoma and were remarkably detected in a cluster in surrounding non-tumorous lesions adjacent to hepatoma. From persistent retension of HBsAg and proliferation of HBV in hepatocytes, surrounding nontumorous lesions adjacent to hepatoma should be associated with hepatocarcinogenesis.
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  • Immunological studies on asymptomatic HB virus carrier
    Effects of sex hormones on termination of immunological tolerance
    Yoshihiro IKEMOTO, Yasuhiro MIZOGUCHI, Michiru FUKUI, Hiroko KATOH, Hi ...
    1985 Volume 26 Issue 5 Pages 578-581
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.578
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    The immunological tolerance to sheep blood cells was induced in C3H/HeJ mice and C57BL/6 mice but not in Balb/c mice, according to the method of Dietrich et al. This immunological tolerance was terminated partially by injecting estrogen in to the mice. However, the effect of estrogen on the termination of immunological tolerance was inhibited by testosterone.
    These results suggested that immunological tolerance was modulated by sex hormones.
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  • The therapeutic efficacy of antiviral agents and corticosteroid for HBeAg-positive patients with chronic hepatitis: from the behavior of HBeAg concentration in serum
    Kiyohiko KURAI, Shiro IINO, Kazuhiko KOIKE, Yasuo ENDO, Hiroshi OKA, K ...
    1985 Volume 26 Issue 5 Pages 582-591
    Published: May 25, 1985
    Released on J-STAGE: January 19, 2010
    DOIhttps://doi.org/10.2957/kanzo.26.582
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    Serial examination of HBeAg concentration was performed in forty patients treated with antiviral agents and corticosteroid till six months after treatment. In every group of interferon (n=11), adenine arabinoside (n=15), adenine arabinoside 5'-monophosphate (n=5), corticosteroid (n=9), HBeAg concentration markedly reduced in six months after treatment and mean percent changes in its concentration from just before treatment in each group were -46.3%, -45.7%, -30.0%, -66.8%, respectively and those changes in interferon, adenine arabinoside, corticosteroid groups significantly differed from that (-13.8%) in control group (n=15). In all patients cleared HBeAg, HBeAg concentrations were under 1000 units at the start of treatment and mean value of those was significantly lower than that in the patients remained HBeAg positive.
    These result suggest that antiviral and corticosteroid therapy accelerate the clearance of HBeAg from serum and shorten natural course of hepatitis associated with chronic hepatitis B Virus infection.
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  • Diagnostic value of liver scintigraphy in fulminant hepatitis and severe acute hepatitis
    Susumu SHIOMI, Naoko IKEOKA, Takami MINOWA, Tetsuo KUROKI, Shigeyoshi ...
    1985 Volume 26 Issue 5 Pages 592-597
    Published: May 25, 1985
    Released on J-STAGE: January 19, 2010
    DOIhttps://doi.org/10.2957/kanzo.26.592
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    Liver scintigraphy was performed in 12 cases with fulminant hepatitis, in 8 cases with severe acute hepatitis and in 44 cases with acute hepatitis.
    Scintiphotoes of severe hepatitis showed reduction of liver size, marked visualization of the bone marrow and the spleen, so this pattern was useful to differentiate from acute hepatitis.
    Relative size of the liver calculated by A.L.I. (anterior liver index) showed significant reduction in severe hepatitis compared with that of acute hepatitis.
    Three of five patients with died of severe hepatitis showed high uptake in the lung and ribs, but none of fifteen patients with severe hepatitis who recovered showed the abnormal accumulation in the lung and in the ribs.
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  • Three dimensional organization of cytoskeleton of hepatocytes in mouse
    Comparative study with rat cytoskeleton
    Masaharu OHTA, Takeshi OKANOUE, Ongyoku OU, Kazutomo KACHI, Tadao OKUN ...
    1985 Volume 26 Issue 5 Pages 598-604
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.598
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    Mouse and rat livers perfused with detergent solution containing 0.5% Triton X-100 were studied by transmission and scanning electron microscope. Cytoskeletons of hepatocytes were clearly observed in situ in mouse and rat with transmission and scanning electron microscope. We could visualize three dimensional filamentous networks of cytoskeleton in mouse and rat with scanning electron microscope. Intermediate filaments showed branching and end-to-side contacts, and connected with microtubules, microfilaments, cell organella, and nuclei. No differences were noted in hepatocyte cytoskeleton between mouse and rat. The present scanning electron microscopic technique is thought to be very useful in investigation of cytoskeletal changes in various kinds of liver injuries.
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  • Study on the mechanism of inhibition of chemical hepatocarcinogenesis by polyprenoic acid
    Makoto MURATA, Kiwamu OKITA, Shinji OKA, Shinya TANAKA, Kazunari YAMAM ...
    1985 Volume 26 Issue 5 Pages 605-612
    Published: May 25, 1985
    Released on J-STAGE: January 19, 2010
    DOIhttps://doi.org/10.2957/kanzo.26.605
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    To clarify the effect of polyprenoic acid (E-5166) in the process of hepatocarcinogenesis, these authors investigated cell proliferation kinetics and induction of gamma-glutamyltranspeptidase positive hyperplastic nodule (GGT positive HPN), using 5 groups, different doses and administration periods of E-5166. Analysis of cell proliferation kinetics using autoradiography (3H-thymidine) and induction of GGT positive HPN were investigated in every week until 5th week, in application of Solt & Farbers' model.
    In the small dose group administered in early period, DNA synthesis and induction of GGT positive HPN were suppressed exceedingly.
    On the other hand, in the process of normal hepatic regeneration, DNA synthesis was retarded and the time of residual hepatocytes converting G0, G1, to S phase was elongated in thegroup administered E-5166 beforehand.
    These observations suggest that E-5166 is a potent agent for inhibition of cell proliferation in the processes of hepatocarcinogenesis and normal hepatic regeneration.
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  • Sialyltransferase is excreted by hepatic carcinoma tissue resulting in high levels in circulating blood
    Shinjiro AKIYAMA, Yushiro WATANABE, Shoichi SHIMIZU, Kazuhiko OKABE
    1985 Volume 26 Issue 5 Pages 613-617
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.613
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    It has been shown that the sialyltransferase activity in serum and in plasma from cases of hepatocellular carcinoma and cancer metastatic to the liver is markedly high. This study was made to elucidate the origin of increased sialyltransferase activity in blood by means of a tumorbearing mouse experiment.
    In C3H/He mice to which the MH134 cell line of mouse ascites hepatoma had been transplanted, the enzyme activity was high in serum and hepatic tissue but low in cancer tissue. Examining the results of 48-hour culture of Chang liver cells and the MH134 cell line, higher enzyme activity was observed in the culture supernatant of the latter and in the cultured cells of the former.
    These findings indicate that sialyltransferase is excreted by carcinoma tissue in cases of cancer of the liver, resulting in high levels in circulating blood.
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  • Blood coagulation and fibrinolysis in 146 cases of hepatocellular carcinoma
    Hiroyuki EBATA
    1985 Volume 26 Issue 5 Pages 618-629
    Published: May 25, 1985
    Released on J-STAGE: March 08, 2010
    DOIhttps://doi.org/10.2957/kanzo.26.618
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    Coagulofibrinolytic studies were made in 146 patients with hepatocellular carcinoma (HCC). Coagulation factors, antithrombin III and α2-plasmin inhibitor were fairly well preserved in the HCC patients in comparison with patients of cirrhosis alone. The terminal stage of HCC patients showed hypercoagulable state and increase of fibrinolysis and they were accompanied with tumor enlargement, metastasis, endotoxemia and tumor thrombus.
    Elevation of FDP and positivity of serial dilution of protamin sulfate test were often seen in the cases of wide-spread tumor thrombus. In four patients with tumor thrombus in their right atriums, coagulofibrinolytic tests revealed like as chronic disseminated intravascular coagulation (DIC).
    Six patinents were diagnosed as DIC and they were accompanied with cancer necrosis, infection and factor IX concentrate administration. There were often seen transient elevation of fibrinogen and FDP in patients recieved transcatheter hepatic artery embolization. Only thrombocytopenia was seen in patients given anticancer agents into their hepatic arteries.
    Prothrombin time, hepaplastin test, antithrombin III and α2-plasmin inhibitor were evaluated as indicators of the hepatic synthesis. The present of dysfibrinogenemia was suspected in both HCC and cirrhotic patients.
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  • Kupffer cells in well-differentiated tissue of hepatocellular carcinoma
    Kazuo TOBE, Takahiro TSUCHIYA, Ryoji FUJIWARA, Gotaro YAMADA, Hideo NA ...
    1985 Volume 26 Issue 5 Pages 630-637
    Published: May 25, 1985
    Released on J-STAGE: January 19, 2010
    DOIhttps://doi.org/10.2957/kanzo.26.630
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    Kupffer cells in hepatocellular carcinoma (HCC) was examined by immunohistochemical detection of lysozyme, a marker of Kupffer cells, and by electron microscopy. In 4 of 20 cases, Kupffer cells were found in the cancer nodules with similar frequency as in non-neoplastic liver tissue. Large phagosomes were observed in the cytoplasm of the Kupffer cells by electron microscopy. Kupffer cells were found only in the well-differentiated parts of HCC in which tumor cells showed a one or two-cell-layered plate-like arrangement and the sinusoidal structure was well preserved. Kupffer cells were distributed in the periphery of the nodules or throughout the nodules as a mosaic. The parts of HCC which contained Kupffer cells were compressed by the undifferentiated parts. These findings indicate that Kupffer cells are present in highly differentiated HCC.
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  • Effect of ethanol on the hepatic clearance of serum glycoproteins in the rat
    Yutaka KOHGO, Junji KATO, Takuji NISHISATO, Shuuji MATSUMOTO, Hitoshi ...
    1985 Volume 26 Issue 5 Pages 638-644
    Published: May 25, 1985
    Released on J-STAGE: March 08, 2010
    DOIhttps://doi.org/10.2957/kanzo.26.638
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    Impaired synthesis and secretion of serum glycoprotein has been reported by several investigators. Recently, we found a paradoxical results that senlm α1 acid glycoprotein is rather elevated in chronic alcoholics and this phenomenon led us to investigate the effect of ethanol on the hepatic clearance of serum glycoproteins by experimental model using ethanol fed rats. Male Wistar rats were fed with Liber's ethanol containing diet for 7 days. Te half life of 125I labeled asialo α1 acid glycoprotein in ethanol fed rat was 3.0 minutes, whereas that of normal control was 1.8min. This retardation of the clearance rate was confirmed by the experiment using isolated rat hepatocytes that significant decrease of the number of hepatic asialo α1 acid glycoprotein receptor of alcohol fed rats was observed. These results suggest that an increase of serum α1 acid glycoprotein level in alcoholics is mainly due to the impairement of the asialoglycoprotein uptake by the hepatocytes.
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  • Light and electron microscopic observation of lymphocytes subsets in liver of patients with primary biliary cirrhosis by immuno-peroxidase method using monoclonal antibodies
    Gotaro YAMADA, Ichinosuke HYODO, Takashi NISHIHARA, Koji MANABE, Shige ...
    1985 Volume 26 Issue 5 Pages 645-651
    Published: May 25, 1985
    Released on J-STAGE: January 19, 2010
    DOIhttps://doi.org/10.2957/kanzo.26.645
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    The distribution of lymphocytes subsets was studied by the peroxidase-labeled antibody method using mouse monoclonal antibodies against Leu-1, Leu-2a, Leu-3a, Leu-7 and Leu 10 antigens in liver biopsies from 5 patients with primary biliary cirrhosis (PBC) at the light and electron microscopic levels. Leu-1+ cells (pan T cells), especially Leu-2a+ cells (cytotoxic/suppressor T cells), were frequently demonstrated in interlobular bile ducts with chronic nonsupprative destructive cholangitis (CNSDC). In the ultrastructural observation, Leu-1+ cells and Leu-2a+ cells often migrated into the space between epithelial cells of bile ducts. These lymphocytes often showed broad surface contact against bile duct epithelial cells. Some epithelial cells in contact with lymphocytes showed loss of microvilli or bleb formation on the laminal surface. These findings suggest that cytotoxic T lymphocytes may be associated with CNSDC in PBC.
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  • A case report of HBV carrier after superimposed acute hepatitis type B upon preexisted chronic non-A, non-B hepatitis
    Masashi TAKAHARA, Masayoshi YAMAUCHI, Hajime NAKAYAMA, Masao NAKAHARA, ...
    1985 Volume 26 Issue 5 Pages 652-655
    Published: May 25, 1985
    Released on J-STAGE: January 19, 2010
    DOIhttps://doi.org/10.2957/kanzo.26.652
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    A 31-year-old man suffered from non-A, non-B hepatitis, and serum transaminase have fluctuated over 1 year. Nineteen months after the onset of the disease, liver biopsy was done, which showed chronic active hepatitis. By chance, serological examination revealed positive HBs antigen in serum on about 3 years after the onset. Further serial seological survey by stocked sera showed that the patient was infected with HBV on about 2 years after the onset of acute non-A, non-B hepatitis, and furthermore he became HBV carrier state.
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  • Report of a case of fulminant alcoholic hepatitis
    Kozo MIZUIRI, Toshie YOSHIOKA, Tomoki HATORI, Hiroshi SAGAWA, Tsunehik ...
    1985 Volume 26 Issue 5 Pages 656-660
    Published: May 25, 1985
    Released on J-STAGE: January 19, 2010
    DOIhttps://doi.org/10.2957/kanzo.26.656
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    A 46-year-old female who died of acute hepatic insufficiency due to alcoholic hepatitis with liver cirrhosis is reported. She was admitted because of fever, jaundice and subcutaneous hemorrhage in both thighs and around the hip joints.
    The liver was palpable 2 FB below the right costal margin. She had suffered from disseminated intravascular coagulation for which heparin and gabexate mesilate were given.
    Prednisolone and glucagon-insulin therapy were applied for acute hepatic insufficiency. However, she died from GI bleeding due to DIC on the 42nd day. The liver specimen showed many alcoholic hyalines with micronodular cirrhosis.
    Thirty-seven of the 111 patients (33.3%) with alcoholic hepatitis reported in the Japanese literature have died.
    Of these, reports of females are very few. The incidence of alcoholic hyaline was highest in patients who died of alcoholic hepatitis with liver cirrhosis.
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  • A case of hepatocellular carcinoma with numerous cholesterol crystals in the tumor tissue
    Fuyuhiko NINOMIYA, Genjiro YAMAGUCHI, Kimihiko SHIRAISHI, Yasuhiko KUB ...
    1985 Volume 26 Issue 5 Pages 661-666
    Published: May 25, 1985
    Released on J-STAGE: January 19, 2010
    DOIhttps://doi.org/10.2957/kanzo.26.661
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    A 67-year-old male who had hepatocellular carcinoma with liver cirrhosis showed remarkably low in density in the tumor area by CT scan. The resected tumor measured 6×6×6cm in size and was encapsulated. The patient died of hematemesis resulting from rapture of esophageal varices.
    Microscopic study of the tumor revealed numerous cholesterol crystals. Cholesterol content in the tumor tissue was approximately 8 times compared with normal liver and the concentration of cholesterol ester comprised 36.2% of total cholesterol.
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 5 Pages 667
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.667
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 5 Pages 668
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.668
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 5 Pages 669
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.669
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 5 Pages 670
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.670
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 5 Pages 671
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.671
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 5 Pages 672
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.672
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 5 Pages 673
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.673
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 5 Pages 674
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.674
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 5 Pages 675
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.675
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 5 Pages 676
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.676
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1985 Volume 26 Issue 5 Pages 677
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.677
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    1985 Volume 26 Issue 5 Pages 678
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.678
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  • [title in Japanese]
    1985 Volume 26 Issue 5 Pages 679-688
    Published: May 25, 1985
    Released on J-STAGE: May 26, 2009
    DOIhttps://doi.org/10.2957/kanzo.26.679
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