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Akihiko KIMURA, Masayoshi KAGE, Ken YUGE, Eiichiro ONO
1986 Volume 27 Issue 10 Pages
1363-1370
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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Seroconversion from HBeAg to anti-HBe (SC), HBcAg in liver tissue (liver HBcAg) and liver histology were studied in 11 HBsAg positive patients with chronic hepatitis.
Initial liver specimens were obtained from 8 patients with chronic active hepatitis (CAH) and 3 patients with chronic inactive hepatitis (CIH) during HBeAg positive phase. In 5 patients with chronic hepatitis who had SC, we did follow up liver biopsies during anti-HBe positive phase. After the seroconversion, two of them changed from CAH to CIH, one of them changed from CAH to chronic persistent hepatitis (CPH), however other 2 patients showed no histological changes.
In the initial liver biopsy, we obtained 5 patients with positive liver HBcAG. After the seroconversion, liver HBcAg of 2 patients were changed to negative.
All of 6 patients with negative liver HBcAg showed SC within 10.6 months in average (range: 3-24 months, 2 of them showed SC within 6 months). However 5 patients with positive liver HBcAg showed SC within 22 months in average (range: 14-36 months). The data indicate that the two groups with or without liver HBcAg show different altitude in SC.
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Etsuko HASHIMOTO, Toshimi NAKANISHI, Kingo YOSHIDA, Seken KYU, Takeshi ...
1986 Volume 27 Issue 10 Pages
1371-1375
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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We studied the long-term outcome of hepatitis B (HB) vaccination. Anti-HBs response to booster injection was determined in 41 vaccine recipients, whose anti-HBs titers were below 23 by PHA at the end of the follow-up period. 116, who were seronegative for both HBsAg and antiHBs by RIA, received the HB vaccine contained 20μg of HBsAg at 0, 4 and 24 weeks. During the period 1981 to 1982, in May 1984 and August 1985, a further investigation was carried out (156 week in average). Seroconversion rate to anti-HBs increased 79.3% within 28 weeks at just after the last vaccination. However after a 156 week follow-up period, anti-HBs disappeared in 25, making the seroconversion rate 57.7%, Anti-HBs titers increased with each succeeding inoculation and then decreased during the follow-up period in all vaccinees except one, who seemed to have had a natural infection of HB virus. After a single booster injection a brisk rise of anti-HBs was observed in all of the 18 responders, because of anamnestic response. 70% of low responders and 23.1% of no responders developed anti-HBs respectively. Anti-HBs titers did not remain at a protective level in some vaccinees during the follow-up period. Therefore, booster injections are necessary whenever anti-HBs titers fall below protective level, with a regular follow-up. A single booster is sufficient within the follow-up period since memory of immunization seems to remain in responders.
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Tadao OKUNO, Toshikazu NAKAJIMA, Yoshimichi ABE, Makoto TAKEDA, Takesh ...
1986 Volume 27 Issue 10 Pages
1376-1383
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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Fifteen patients with HBeAg positive chronic type B hepatitis were treated with interferon (IFN) and followed up for 18 to 39 months after end of IFN treatment. Thirteen patients received a single course of IFN-αor IFN-βtreatment and two received two courses of IFN-βand IFN-α, and IFN-βand recombinant IFN-αA treatment, respectively. IFN was given daily for 4 weeks. Total dose of IFN administered was 1×10
8IU to 4.77×10
8IU. Five (33.3%) of 15 patients become seronegative for HBeAg just after treatment and 2 patients (13.3%) seroconverted to anti-HBe. Six patients (40%) lost HBeAg 18 months after treatment and no more patients lost HBeAg after that period. Three (20%) seroconverted to anti-HBe 12 months after treatment and this figure remained unchanged even after 12 months. Six (42.8%) of 14 patients lost DNA polymerase (DNAP) from serum and about 40% of the patients were DNAP negative after 12 months. Two (13.3%) of 15 patients lost HBV DNA from serum just after treatment, but all patients become HBV DNA positive in serum after 18 months. Two patients with persistently high level of anti-HBe showed sustained elevation or fluctuation of SGPT in whom both DNAP and HBV DNA were positive in serum.
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Masaaki SAITO, Kunihiko OHNISHI, Hidetaka TERABAYASHI, Shinji IIDA, Fu ...
1986 Volume 27 Issue 10 Pages
1384-1388
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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Intrahepatic pressure was measured using 23 G Terumo Cathelin needles connected to a pressure transducer in 76 patients with viral liver disease, and compared with histomorphological changes of the liver. Intrahepatic pressure was significantly correlated with the degree of fibrosis, inflammation, necrosis of the liver on light microscopy and the degree of collagen in the Disse space on electron microscopy. Especially, intrahepatic pressure was best correlated with the degree of fibrosis on light microscopy in this study (r=0.67, p<0.001).
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Takashi KUMADA, Sumihiko OKUYAMA, Masami IMOTO, Daisaku NISHIMURA, Yos ...
1986 Volume 27 Issue 10 Pages
1389-1397
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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In this study, we present a method of measurement of sulfated bile acids by enzymatic hydrolysis using sulfatase, and measure concentration of sulfated bile acids in serum samples of 10 normal subjects and 81 patients with hepatobiliary diseases.
Serum levels of sulfated bile acids increased in patients with acute hepatitis, extrahepatic or intrahepatic cholestasis. And the percentage of sulfated bile acids to total bile acids in serum samples of patients with fulminant hepatitis or decompensated liver cirrhosis was significantly lower than that in serum samples of patients with acute hepatitis or compensated liver cirrhosis (p<0.01). There were also positive correlation between the percentage of sulfated bile acids in sera and thrombotest, normotest, prothrombin time and α
2-heat-stable-glycoprotein (p<0.01 or p<0.05).
From these results, it is considered that measurement of serum levels of sulfated bile acids can be efficient indicator in the diagnosis of the development of liver damage.
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Shoichi SUEMORI, Eiichi TAKEZAKI, Ikuo MATUO, Sigeru NAKAYAMA, Toshio ...
1986 Volume 27 Issue 10 Pages
1398-1404
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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In previous experimental study, we reported galactose metabolism in regenerating rat liver.
In this paper, we studied galactose tolerance test in GalN treated rat liver. 2 days after GalN treatment, galactose tolerance test (T1/2) was most prolonged and strongly influenced by liver tissue galactokinase and glucose-6-phosphatase activity.
There were some daily changes in hepatic utilization of galactose for glycogenesis, glycogenolysis and glycolysis due to influence of degree of liver damage and peripheral glucose metabolism in GalN treated rats.
From these results, it was suggested that galactose metabolism was controlled to sustain the homeostasis of carbohydrate metabolism against the disorder of carbohydrate metabolism induced by liver damage.
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Hideo MIZUYOSHI, Koji ISHII, Wataru YAMAMURO
1986 Volume 27 Issue 10 Pages
1405-1411
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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Sulfobromophthalein (BSP) 5mg/kg was injected as a bolus to 27 cases with obstructive jaundice.
Blood and bile sumples were taken for 120 minutes in 10-15min interval and serum BSP was fractionated by sirica-gel chromatography. Bile was collected via PTCD.
Three fractions of BSP were separated from serum and bile, which were identified as free (FBSP), cysteine conjugated (Cyst-BSP) and glutathione conjugated (GSH-BSP) BSPs.
The higher the serum bilirubin, the lower was serum total BSP disappearance rate (K). The retention of serum BSP was attributed to an increase in Cyst-BSP, which account for some 75-82% of total BSP at 120 minutes after the administration.
In the bile specimen, F-BSP and Cyst-BSP were the major components of total BSP.
The results suggested that, in obstructive jaundice, the major conjugate of BSP which regurgitated in the blood stream and excreted into bile, is cysteine conjugated, due probably to an impaired metabolism of GSH-BSP.
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Indication of extensive intrahepatic shunts
Hideo TANAKA, Kunihiko OHNISHI, Shingi CHIN, Hidetaka TERABAYASHI, Mas ...
1986 Volume 27 Issue 10 Pages
1412-1417
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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On the premise that extensive intrahepatic portal-venous anastomoses known to occur in cirrhosis would be demonstrated by contrast medium directly placed in the portal vein, percutaneous transhepatic portograms were analyzed in 82 patients with liver cirrhosis in relation to the estimated degree of shunting. The degree of intrahepatic shunt was measured during transhepatic portography using
99mTc-macroaggregated albumin. Hepatic veins began to opacify at 4 to 10sec after the start of injection of contrast medium during portography in 20 patients with cirrhosis who had intrahepatic shunt indices of 58.5±18.5% but it was not visualized in 62 patients with cirrhosis whose shunt indices were 19.9±14.1% Anomalous large anastomoses of 1 or 2mm in size between the right portal vein and the right hepatic vein were clearly visualized during portography in 18 of 20 patients in whom the hepatic vein was opacified. Frequency and time of beginning opacification of the hepatic vein were closely correlated with the degree of intrahepatic shunt. Thus, opacification of the hepatic vein and abnormal anastomoses between the portal and hepatic vein systems in an early phase of portography indicates extensive intrahepatic shunts.
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Junko MORI, Masami TAGAWA, Masao OMATA, Kunio OKUDA, Kiyoshi TERAO
1986 Volume 27 Issue 10 Pages
1418-1423
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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We obtained liver tissue from 21 ducklings (2 or 3 ducks each sacrificed at 6, 12, 24, 36 hr, 3, 6 and 10 day, one positive and one negative control) which were inoculated serum containing duck hepatitis B virus. We examined DHBV DNA by in situ hybridization and compared the result with the number of copies of extracted DHBV DNA obtained by spot hybridization. Significant amount of DHBV DNA were first observed at day 3 by in situ hybridization. The mean number of grains were 4.6 at day 3; 24.3 at day 6; and 48.3 at day 10. The coefficient of correlation between grains/cell and extracted DNA/cell was 0.96. We calculated that each DNA copy was represented by approximately two grains. We conclude that the result obtained by in situ hybridization technique are closely correlated with the amount of viral DNA extracted from hepatocytes.
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Kenji IKEDA, Hiromitsu KUMADA, Yasuji ARASE, Ikuo NAKAMURA, Yasumi NOZ ...
1986 Volume 27 Issue 10 Pages
1424-1430
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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One hundred and sixty-one patients with hepatocellular carcinoma was studied. Fifteen (9.3%) out of them had no liver cirrhosis. Incidense of patients with Hepatitis B surface antigen in the serum was slightly higher in noncirrhotic cases (6/15, 40.0%) than cirrhotic (31/146, 21.2%), but no significant difference was found in age, sex, history of blood transfusion, and amount of alcohol intake.
Histology of noncancerous part of liver in HBs antigen positive cases were liver fibrosis in 4 and chronic persistent hepatitis in 2. While in HBs antigen negative cases, chronic aggresive hepatitis 2A in 4, chronic aggressive hepatitis 2B in 2, liver fibrosis in 2, and normal liver in 1. Thus activity of liver disease was quite different between those with and without HBs antigen in the serum.
Five patients under medical follow up were successfully treated by hepatectomy in 3, and by transcatheter arterial embolization in 2. In other ten patients without medical service, only one was treated with surgery and two with embolization therapy. If cases with HBs antigen positive liver disease and cases with HBs antigen negative chronic hepatitis will be followed up medically, we can perform more useful treatment for patients with hepatocellular carcinoma without liver cirrhosis.
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Toshihisa NAKAHARA
1986 Volume 27 Issue 10 Pages
1431-1438
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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Combined hepatocellular and cholangiocarcinoma (CHC) is a rare form of primary liver cancer, and its clinicopathologic features and histogenesis are not well-documented yet.
Clinicopathologically, the author studied 10 cases of CHC seen among 393 autopsy cases of primary liver cancer at the Pathology Department of Kurume University during the past 10 years. Average age, sex ratio, clinical symptoms, and biochemical data were not significantly different from those of hepatocellular carcinoma (HCC). However, serum alpha-fetoprotein levels were relatively low and were within 10000ng/ml at the maximum. Serum CEA was positive in 88% of the cases. According to Namashima's gross classifications, eight cases were of an infiltrative type, one case mixed infiltrative and expansive type, and one diffuse type. There were no cases of encapsulated type which is common in HCC. Liver cirrhosis was associated in 5 cases (50%). Among the 10 cases, transitional features between HCC and CHC were histologically observed in 3 cases. One case was confirmed as double cancer evidenced by separate localization of HCC and CHC. Hematogenous metastasis, mostly in the lung, consisted exclusively of the HCC component, while lymphatic metastasis consisted mostly of cholangiocarcinoma. The simultaneous metastasis of both components was found in the peripancreatic lymph node of one case.
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Keiki OGINO, Isao SAKAIDA, Shinji OKA, Hujio MURAKAMI, Keisuke HINO, K ...
1986 Volume 27 Issue 10 Pages
1439-1443
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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Anionic form of glutathione S-transferase (GST-π) was purified in apparent homogenity from human placenta. The molecular weight of GST-π was 21, 000 by SDS-polyacrylamide gel electrophoresis and isoelectric point was 4.5 by analytical electrophocussing gel electrophoresis. According to ouchtrlony's method, antigenicity of GSY-π was identical with anionic GST from hepatocellular carcinoma and kidney. Immunohistochemical localization of GST-π in 30 cases of hepatocellular carcinoma revealed that positive staining was shown in 50% cases compared to Edmondson's grade I or II. It was interesting that hepatocytes around cancer tissue, not found out atypism, were stained intensively sometimes. Therefore, it is suggested that GST-π may be tumor marker for hepatocellular carcinoma. However, the investigation of GST-π rich hepatocytes around cancer tissue is necessary in connection with pre-cancerous lesion.
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Katsuji KOHIGASHI, Yoshihiro FUKUDA, Hiroo IMURA, Hiroshi NAKANO
1986 Volume 27 Issue 10 Pages
1444-1450
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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Male Sprague-Dawley rats were given a single intraperitoneal injection of diethylnitrosamine (DEN, 200mg per kg of body weight). Since 4 weeks after injection the rats were fed diethylstilbestrol (DES, 1.8mg per day) for eight months. Rats given DEN and DES developed grossly visible hepatic hyperplastic nodules. Total area and mean area per foci of γ-glutamyl transpeptidase-positive foci in the liver were significantly larger in the rats given DEN and DES than the rats given DEN or DES alone. Total estrogen receptor (ER) contents in liver cytosol determined by enzyme immunoassay were significantly larger in rats given DES for 3.5 months than those in rats not given DES. However unoccupied ER contents determined by radioreceptor assay were significantly smaller in rats given DES for eight months than those in rats not given DES. It is suggested, therefore, that DES can promote hepatocarcinogenesis mediated by ER in the rat.
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Shigeru SUZUKI, Kazuo MASUKO, Chikao YAMAZAKI, Takehiro MITSUI, Mitsug ...
1986 Volume 27 Issue 10 Pages
1451-1456
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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A 28-year-old man was suffered from high fever, general fatigue and losing appetite toward the end of June, 1983. The serological examination revealed positive both IgM-HA antibody and HBs antigen with high titer of HBc antibody. He showed markedly prolonged prothrombin time and increase in serum TTT, IgM as well as atypical lymphocytes in blood. Based on the findings described above, a diagnosis of fulminat hepatitis type A with superimposed infection upon asymptomatic HBV carrier was made.
Although plasma exchange and glucagon-insulin therapy on admission were performed, no effect was seen in prolonged prothrombin time and hepatic coma progressed. He died on the 5th day after admission because of disseminated intravascular coagulation (DIC).
Serological re-examination proved high titer of δ antibody. In the liver tissue from necropsy, δ antigen was recognized in a few hepatocyte nuclei by means of immunoperoxidase technique.
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Shinichi AOKI, Shozo SAITO, Yoshiki OZAKI, [in Japanese], [in Japanese ...
1986 Volume 27 Issue 10 Pages
1457-1462
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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A 38 year-old woman was admitted to Kyorin University Hospital because of melena on Oct. 22, 1984. Her past history revealed appendectomy and Cesarian section The patient had suffered from liver cirrhosis and diabetes millitus for 5 years.
Endoscopic examinations revealed to bleeding focus in the esophagus, duodenum, colon and rectum, although sever esophagus varies were seen. Superior mesenteric angiography showed a dilated mesenteric vein which was suggestive of Jejunal varies.
Laparotomy was done on Dec. 25, and confirmed the rupture of Jejunal varies. Her past operative course was uneventful varies in the small intestine are very rare and only 16 cases were so far reported in Japan.
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With reference to the results of analysis of amyloid protein
Masaji NAMBU, Shotani MAEDA, Toshihiko IIJIMA
1986 Volume 27 Issue 10 Pages
1463-1469
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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A 46-year-old man with primary liver amyloidosis associated with jaundice (T-bilirubin 14.3mg%) was described. The patient had had gallstone and been put on bile acids therapy (CUDCA+CDCA). Jaundice developed after the therapy, and this made it impossible to rule out a causal relationship between jaundice and the therapy.
The patient died from a profuse bleeding in the abdominal cavity on the tenth hospital day. Autopsy revealed primary amyloidosis associated with rupture of liver cavernous angioma. Cause of death was a bleeding due to the ruptured hemangioma.
Amyloid protein was analysed, and it was discussed.
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
1986 Volume 27 Issue 10 Pages
1470
Published: October 25, 1986
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
1986 Volume 27 Issue 10 Pages
1471
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
1986 Volume 27 Issue 10 Pages
1472
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
1986 Volume 27 Issue 10 Pages
1473
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
1986 Volume 27 Issue 10 Pages
1474
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
1986 Volume 27 Issue 10 Pages
1475
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
1986 Volume 27 Issue 10 Pages
1476
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
1986 Volume 27 Issue 10 Pages
1477
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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1986 Volume 27 Issue 10 Pages
1478-1506
Published: October 25, 1986
Released on J-STAGE: July 09, 2009
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