Kanzo

Detection of serum HCV antibodies (JCC, SP42, C-100) in Non-A, Non-B and other various liver diseases

A comparison among the serum JCC, C-100 and SP42 antibody

Yasuyuki ARAKAWA, Junko MORIYAMA, Mitsuhiko MORIYAMA, Naohide TANAKA, ...

1992 Volume 33 Issue 10 Pages 735-744
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.735

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The blood JCC antibody levels in patients with non A non B (NANB) and other liver diseases were determined by a JCC ELISA system prepared from 120 amino acids in the HCV core region to compare the occurrence of JCC, C-100, and SP42 antibodies among patients with different liver diseases. Studies were carried out on 54 patients with NANB-acute hepatitis, 316 patients with chronic hepatitis, 132 patients with liver cirrhosis, 57 patients with HCC, and 121 patients with other liver diseases. In part of the study population blood HCV-RNA levels were also determined. The positive rate in NANB-CH was 10% to 30% higher with JCC than with other antibodies. Regarding the coincidence with HCV-RNA values, JCC antibody have an advantage over other antibodies. JCC antibody was detected in about 30% of patients with alcohlic liver disease and autoimmune hepatitis. JCC antibody can therefore be a very useful marker for hepatitis C. The rate of JCC positive was lower in the LC and HCC groups than in the CH group. This finding is intriguing from the standpoint of a possible relationship of JCC antibody to HCV proliferation and HCC development.

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Epidermal growth factor (EGF) receptor level in the liver of an experimental acute hepatic failure model and effect of EGF on the hepatocytotoxicity of Propionibacterium acnes-elicited hepatic adherent cells

Toru KIDA, Yasuhiro MIZOGUCEI, Ichiro SHIRAISHI, Kenzo KOBAYASHI, Seij ...

1992 Volume 33 Issue 10 Pages 745-750
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.745

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When a small amount of lipopolysaccharide (LPS) was intravenously injected into mice which had been injected with heat-killed Propionibacterium acnes (P. acnes) 7 days before, acute hepatic failure was induced and most of the mice died within 24 hours. However, when EGF was administered with LPS, remarkable improvements in the survival rate and in the histological changes of the liver were observed. In this experiment, we studied the EGF receptor level and the effect of EGF on the hepatocytotoxicity of P. acnes-elicited hepatic adherent cells in order to find out how EGF suppressed the induction of acute hepatic failure in this experimental model. As a result, EGF receptor level was decreased to about 60% of normal control at 12 hours and 18 hours after LPS injection. Furthermore, EGF protected hepatocytes from the cytotoxicity of P. acnes-elicited hepatic adherent cells.

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Evaluation of BrdU labeling index as a prognostic factor of hepatocellular carcinoma

Shinichi OHKAWA, Yoshihiko ITO, Setsuo TAMAI, Kazuo TARAO, Yukio SUGIM ...

1992 Volume 33 Issue 10 Pages 751-757
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.751

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In order to study the relationship between prognosis of hepatocellular carcinoma (HCC) and DNA synthetic potency of HCC and LC, BrdU labeling indices of cancerous and non-cancerous portion in 14 hepatectomised cases were examined, using an in vitro immunostaining technique, and followed up for more than 2 years after hepatectomy. The results were as followed: 1) BrdU L.I. of cancerous and non-cancerous portion were 6.43±0.89% (Mean±SE) and 2.85±0.42%, respectively. There was a significant positive correlationship between L.I. of cancerous and non-cancerous portion. 2) There was a negative correlationship between L.I. of cancerous ponion and the periods of survival time. There was also a negative correlationship between L.I. of non-cancerous portion and survival time, which means that HCC patients with LC in whom L.I. are low could obtain good prognosis. 3) It was demonstrated that BrdU L.I. of both HCC cells and background hepatocytes of liver cirrhosis was a useful prognostic factor in hepatocellular carcinoma.

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Growth speed of hepatocellular carcinoma

Relationship with arterial vascularity evaluated by US angiography

Hitoshi TOCHIO, Syusuke TOMITA, Masatoshi KUDO, Jun MIMURA, Michio HAM ...

1992 Volume 33 Issue 10 Pages 758-765
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.758

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Relationship between arterial vascularity and tumor growth speed was evaluated in 41 hepatocellular carcinomas (HCC). Arterial vascularity of HCCs was classified into 3 patients with US angiography during intraarterial carbon dioxide microbubbles: hypervascular (n=25), isovascular (n=7), and hypovascular (n=9) HCCs. Tumor growth speed was determined by the tumor volume doubling time (TVDT) with measuring by US. The logarithmic mean of the doubling time in hypervascular HCCs was statisticaly more rapid than that in isovascular or hypovascular HCCs (p<0.05, p<0.001) respectively. Furthermore, the logarithmic mean of TVDT in isovascular HCCs was more rapid than that in hypovascular HCCs (p<0.05). We concluded that positive correlation between tumor vascularity and the tumor growth speed was observed, i.e., the more vascular the tumor is, the more rapid the growth speed becomes.

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The Expression of hepatocyte growth factor in woodchuck hepatocellular carcinomas

Atsushi SHIMODA, Shuichi KANEKO, Masashi UNOURA, Toshikazu NAKAMURA, K ...

1992 Volume 33 Issue 10 Pages 766-769
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.766

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Amplification and rearrangement of hepatocyte growth factor (HGF) gene and expression of HGF RNA were studied using woodchuck hepatocellular carcinomas to understand relationship between HGF and hepatocarcinogenesis. A total of 30 liver tumors and 10 normal liver tissues were obtained from three woodchucks with woodchuck hepatitis virus (WHV) surface-antibody positive and nine woodchucks with WHV. In the digested samples of the ten tumors with Eco RI. Southern blotting showed discrete bands of 2.4, 4.6 and 9kb in common. Expression of HGF RNA was observed in 26 of 30 woodchuck liver tumors. Three of them had higher expression of HGF RNA in liver tumors than expression in normal liver tissues. So far as we studied, HGF gene was not intensified nor rearrnaged clearly but preserved well in woodchuck liver tumors.

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An experimental study of hormonal therapy for experimentally induced rat liver tumor

Masatake TAMAI

1992 Volume 33 Issue 10 Pages 770-778
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.770

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Hormonal dependency and the possibility of hormonal therapy for hepatocellular carcinoma (HCC) has been examined. A total of 108 male rats of the Sprague-Dawley strain were used as experimental animal. Rat liver tumor induced by acetylaminofluorene (AAF) for a short term (10 weeks) was unaffected by estrogen but was affected by androgen. Either antiprogesterone agent (RU486) or RU486 and antiestrogenic agent (tamoxifen; TAM) inhibited the occurrence of AAF induced liver tumor. However, the liver tumor induced by long term administration of AAF (34 weeks) was not inhibited by RU486 and TAM. After castration, RU486 and TAM inhibited the liver tumor induced by AAF. In in vitro experiment, RU 486 was proved to cause inhibition of DNA synthesis and decrease of androgen receptor in HepG2 cells. The inhibitory effect of RU486 and TAM acted via the androgen receptors. Such hormonal therapy may be beneficial for patients with HCC.

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Functional differences between rat Kupffer cells and splenic macrophages

Yoshihiro SHIMAUCHI

1992 Volume 33 Issue 10 Pages 779-786
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.779

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In the present study, functional differences between rat Kupffer cells (KC) and splenic macrophages (SM) was studied by examining the phagocytic activity of latex particles and OK-432, secretion of tumor necrosis factor (TNF), and cell surface expression of Ia antigen in these cells. In vitro studies, the number of phagocytosed latex particles, activities of TNF, and proportion of OX17 antibody POsitive cells of cultured KC were 20.0±10.1 particles/cell, 17.1±8.1 IU/ml/l×106cells, and 36.6%, and those of cultured SM were 11.5±5.9 particles/cell, 86.8±45.8 IU/ml/l×106cells, and 67.1%. In regard to latex phagocytosis, KC was superior to SM, and conversely SM was superior to KC regarding TNF activities and proportion of OX17 positive cells. In vivo studies, the number of phagocytosed latex particles administered intraarterially was significantly higher in KC than in SM. The serum TNF activities induced by LPS. administration were suppressed by splenectomy. From these results, the functional differences was observed between KC and SM, and it might be the sight of adaptation of macrophages to associated organs.

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Investigation of two cases that died from fulminant hepatitis B and acute hepatitis B (severe type) following transfusion with HBsAg-negative blood by R-PHA

Takao SHIBAYAMA, Takeshi TANAKA, Seishuu HAYASHI, Shunich SAEKI, Masan ...

1992 Volume 33 Issue 10 Pages 787-793
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.787

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We experienced two cases that died from fulminant hepatitis B and acute hepatitis B (severe type) following transfusion with HBsAg-negative blood examined by R-PHA. The results of R-PHA examination of the donor blood retrospectively showed that all of the containers of preserved blood were HBsAg-negative, but that one container for patient transfusion used in case 1 was HBsAg- positive, having a cut-off index of 5.58 as examined by RIA, and that that of the other used to transfuse case 2 had a cut-off index of 2.93 in HBsAg value. Both donor bloods had a high titer of anti-HBc as examined by IAHA and had a negative HBeAg and positive anti-HBe as revealed by RIA. Therefore, both of these bloods were considered to have caused the resulting type-B post-transfusion hepatitis. Furthermore, HBeAg was not recognized through the clinical course in both cases following post- transfusion hepatitis. Thus, the sequence of the precore region on HBV-DNA was analyzed for the transfused bloods causing the type-B post-transfusion hepatitis and for the patients' sera of both cases. As a result, all of the obtained HBV are HBV mutants that were found to be defective in the precore region of the HBV genome and to be incapable of encoding HBe antigen. Moreover, it was clear in one case that the sequence of the precore region on HBV-DNA from this patient corresponded well with that from the blood donor. Specifically revealed was a point mutation from guanine to adenine at nucleotide 83 in the precore region of HBV-DNA, converting codon 28 for tryptophan (TGG) to a stop codon (TAG).

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A case of acute brucellosis associated with cholestatic jaundice

Koichi AKAGI, Tetsuo MORISHITA, Soichiro MIURA, Masaya ODA, Hiromasa I ...

1992 Volume 33 Issue 10 Pages 794-799
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.794

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A rare case of acute brucellosis associated with cholestatic jaundice was reported. The patient was 39 years old paraguayan male, admitted to the Universidad Nacional de Asuncion, with fever and jaundice. The body temperature was 39.5°C, and jaundice, hepato-splenomegaly and lymphadenopathy were noted. The laboratory data demonstrated conjugated hyperbilirubinaemia, elevated GOT, GPT and ALP levels, positive Brucella agglutination test, and positive blood culture for Brucella sp. Ultrasonography, ERCP and laparoscopy showed no evidence of biliary tract obstruction. The hepatic histology revealed cholestatic hepatitis. The antibiotic therapy induced a good clinical and biochemical response.

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A case of chronic hepatitis C with drug-induced cholestatic hepatic disorder during interferon administration

Yukiya HAKOZAKI, Tatsuoki SHIRAHAMA, Mashashi KATO, Takahiro FUJIOKA, ...

1992 Volume 33 Issue 10 Pages 800-804
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.800

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The patients was a 44-year-old male who had received drugs such as sodium aurothiomalate and indomethacin due to rheumatoid arthritis since 1988. In 1989, the diagnosis of chronic hepatitis C was made. Administration of interferon (IFN) α-2b 1MU/day (three times weekly) was commenced on June, 1991. During the IFN treatment jaundice appeared. The patient was hospitalized and all medications were discontinued. Serum transaminase level changed within 100IU. Total bilirubin was markedly elevated on the 10th day of hospitalization (Max. 19.66mg/dl). However, these values subsequently decreased. Liver biopsy revealed drug-induced cholestasis with hepatitis. Lymphocyte stimulation test was negative for all administrered drugs during past three years, but IFN-induced cholestatic hepatic disorder was strongly suspected in view of the clinical course.

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Genotypical classification of hepatitis C virus genome with polymerase chain reaction using a mixed primer set

Kazuaki CHAYAMA, Yasuji ARASE, Akihito TSUBOTA, Satoshi SAITO, Kenji I ...

1992 Volume 33 Issue 10 Pages 805-806
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.805

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Relation between type 2 autoimmune hepatitis and HCV infection

Hiroshi MIYAKAWA, Makoto KAKO, Hisashi NAKAMURA, Kazuhiro ABE, KOZO NA ...

1992 Volume 33 Issue 10 Pages 807-808
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.807

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1992 Volume 33 Issue 10 Pages 809
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.809

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1992 Volume 33 Issue 10 Pages 810
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.810

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

1992 Volume 33 Issue 10 Pages 811
Published: October 25, 1992
Released on J-STAGE: July 09, 2009

DOIhttps://doi.org/10.2957/kanzo.33.811

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