We developed an FIA system equipped with a chemiluminescence detector using a mixed chemiluminescence reagent of luminol and 1,10-phenanthroline for the detection of metal ions and metal complexes. The carrier, mixed chemiluminescence reagent comprising luminol, 1,10-phenanthroline, and cethyltrimethylammonium bromide, and H
2O
2 solutions were fed by corresponding pumps at a definite flow rate. Sample solutions dissolving hematin, [Co(NH
3)
4(H
2O)
2]
2(SO
4)
3, CuSO
4, NiCl
2, K
3[Fe(CN)
6], and K
4[Fe(CN)
6] were analyzed as models by the means of the present FIA system. Solutions of hematin, [Co(NH
3)
4(H
2O)
2]
2(SO
4)
3, CuSO
4, and NiCl
2 were detected as positive peaks, as usual. The order of the catalytic activity of these samples for the present chemiluminescence reaction using the mixed chemiluminescence reagent was [Co(NH
3)
4(H
2O)
2]
2(SO
4)
3 > hematin > CuSO
4 > NiCl
2. On the other hand, sample solutions of K
3[Fe(CN)
6] and K
4[Fe(CN)
6] were detected as negative peaks and were determined over the ranges of 1 × 10
-8 - 1 × 10
-6 M with a detection limit of 1 × 10
-8 M and 2 × 10
-8 - 4 × 10
-6 M with a detection limit of 2 × 10
-8 M, respectively. Their negative peaks were observed reproducibly with a relative standard deviation of 2 - 5%.
View full abstract