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58 巻, 7 号
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thymidinekinase活性およびprogesterone receptor今村 好久1982 年 58 巻 7 号 p. 867-875
発行日: 1982/07/20
公開日: 2012/09/24
ジャーナル フリーHypothyroidism has been believed to affect the female reproductive system, but relatively few studies have been made on the direct effects of the thyroid hormone on the uterus, and the results have been conflicting. In the present paper, thymidine kinase (TK) and progesterone receptor (PrgR) in cytosol were assayed in the rat to study the uterine response to estrogen. Furthermore, this uterine response to estrogen was compared between hypothyroid and euthyroid rats. Hypothyroidism was induced by surgical operation performed at 21 days of age.
Immature rats : Estradiol (E2) was injected on day 26. No significant difference in increases of uterine wet weight, TK and PrgR was noted between hypothyroid and euthyroid rats 30 h after E2 injection.
Adult rats : Ovariectomy was added at 21 days or 65 days of age. E2 was injected on day 70. No significant difference in increases of uterine wet weight, TK and PrgR was noted between hypothyroid and euthyroid rats 24 h or 48 h after E2 injection.
These results suggest that hypothyroidism may have no direct effects on such uterine responses to estrogen as wet weight, TK and PrgR.抄録全体を表示PDF形式でダウンロード (797K) -
森岡 政明, 武田 克治, 光畑 直喜, 大橋 輝久, 大森 弘之, 斎藤 利興, 神戸川 明1982 年 58 巻 7 号 p. 876-885
発行日: 1982/07/20
公開日: 2012/09/24
ジャーナル フリーEndogenous androgen levels of prostatic tissues in patients with benign prostatic hypertrophy (BPH) and untreated prostatic cancer (PC) were determined by radioimmunoassay using sephadex LH-20 column chromatography. By this assay procedure, it was possible to measure tissue androgen levels with only 30-40 mg weight of tissues.
In BPH (n=20) the concentrations of steroids (mean ± standard deviation, ng/g. tissue weight) were 5α-androstane 3α, 17β diol (A-diol), 2.31 ± 1.29; dihydrotestosterone (DHT), 4.97 ±2.17; testosterone (T), 0.62 ± 0.33. In PC (n=17) the concentrations were 5.51 ± 3.23; 3.94 ± 3.16; and 0.93 ± 0.38; respectively. The concentrations of DHT were raised and the levels of A-diol were reduced significantly (P<0.01) in BPH compared with PC. Tissue DHT levels of PC were variable, but they could be classified in to two groups. One was below 2 ng levels (n=8) similar to those of non-androgen target tissues and the other was over 2 ng (n=19). The relationships between histological differentiation and tissue DHT levels in prosatic cancer were examined. In poorly differentiated cancer (n=4), DHT levels were low in all cases, furthermore even in well differentiated ones there were 4 cases with levels below 2 ng. Thus, the measurement of endogenous DHT levels of prostatic tissues was considered to be valuable for the judgement of androgen dependency in prostatic cancer.抄録全体を表示PDF形式でダウンロード (934K) -
田中 敏章, H.G. FRIESEN1982 年 58 巻 7 号 p. 886-893
発行日: 1982/07/20
公開日: 2012/09/24
ジャーナル フリーThe human growth hormone (hGH) and human prolactin (hPRL) were iodinated with 125I using the lactoperoxidase method and purified by gel filtration on a Sephadex G-100 column and by ion exchange chromatography on a diethyl-aminoethyl (DEAE) cellulose column. Aliquots of the peak fraction on the Sephadex G-100 column and each fraction on the DEAE cellulose column were tested by radioimmunoassay (RIA) using 131I-hGH or 131 I-hPRL as a tracer and by bioassay using Nb2 rat lymphoma cells.
The ratio of the bioassay and RIA estimates of 125I-hGH was 0.89 ± 0.15 (mean ± SD) and that of both estimates of 125I-hPRL was 1.14 ± 0.16. These data suggest that the lactoperoxidase iodination method is sufficient for labeling hGH and hPRL to a high specific activity without altering their biological integrity.抄録全体を表示PDF形式でダウンロード (616K) -
伊藤 正毅, 山田 幸男, 岩永 敏彦, 柴田 昭1982 年 58 巻 7 号 p. 894-902
発行日: 1982/07/20
公開日: 2012/09/24
ジャーナル フリーIn order to examine whether somatostatin-28 originally isolated from porcine and ovine tissues was present in the human gut and pancreas or not, immunohistochemical methods using specific antisera against somatostatin-28 and somatostatin-14 antisera were carried out in the fetal and adult human gut and pancreas. The specific antisera against somatostatin-28 were prepared from somatostatin-28 antisera by absorption of somatostatin28 antisera with sepharose 4B-somatostatin-14. Somatostatin-like immunoreactivity appeared in the epithelial endocrine-like cells of the gut and in the pancreatic islet cells at 11 week's gestation, and thereafter it was constantly present in the fetal and adult gut and pancreas. Although detection of somatostatin-28 like immunoreactivity was not inhibited in the sections in which specific antisera against somatostatin-28 pretreated with either somatostatin-14 or other kinds of peptide hormones were employed to replace primary antisera in the immunohistochemical staining process, it was completely inhibited after use of the specific antisera against somatostatin-28 absorbed with somatostation-28. This finding suggested that detection of somatostatin-28 like immunoreactivity was due to the immunohistochemical reaction between specific antisera against somatostatin-28 and somatostatin28 itself. Furthermore, observation of serial sections stained by using specific antisera against somatostatin-28 and somatostain-14 antisera revealed that cells reacting with the former antisera were identical to those reacting with the latter one. Although the finding seemed to imply that full sequences of somatostain-28 may be present in the human gut and pan creas, it remained obscure whether or not somatostatin-28 and somatostatin-14 like immunoreactivities may be present independently in the cells since somatostatin-14 antisera have a crossreactivity to somatostain-28.抄録全体を表示PDF形式でダウンロード (3156K) -
西村 進1982 年 58 巻 7 号 p. 903-912
発行日: 1982/07/20
公開日: 2012/09/24
ジャーナル フリーScatchard analysis is currently used to investigate the properties (binding affinity and number) of insulin receptors. In most cases, the Scatchard plots were curvilinear with an upward concavity. This has been explained by the presence of at least two classes of binding sites with different fixed affinities or negatively cooperative interactions between receptors. In this study, we have attempted to compare the properties of insulin receptors in adipocytes of different sizes taken from one adipose tissue region of the same individual rat. Adipocytes from epididymal fat pads, isolated by collagenase treatment, were separated into two different sizes with a technique using two nylon meshes. Small adipocytes were obtained by a filtration process resulting from the floatation through 49 μ nylon mesh. Large adipocytes were obtained from residual adipocytes, which could not penetrate through 70μ nylon mesh. By this method, large adipocytes were more homogeneous in size as compared with small adipocytes. The properties of both groups of cells were investigated by insulin binding and insulin dissociation studies. As a result, insulin binding per cell increased, and the binding was 1.5 times higher in the large adipocyte than in the small with a tracer concentration of insulin. Furthermore, Scatchard plots in insulin receptors from large homogeneous adipocytes developed a tendency to linearize and were mostly accounted for by a high affinity component (Kd 1-2 × 10-10 M/L). On the other hand, Scatchard plots in insulin receptors from small adipocytes showed a curvilinear shape. As is usually seen, negatively cooperative interactions between receptors were demonstrated in small and large adipocytes, and the magnitude of this effect was almost the same in both groups of cells. These results suggest that Scatchard plots may be linearized by entirely homogeneous adipocytes, leading to the conclusion that the curvilinear aspect of Scatchard plots should be the result of fat cell size heterogeneity in insulin-receptor interactions.抄録全体を表示PDF形式でダウンロード (1350K)
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