巻号一覧
前身誌
63 巻, 3 号
選択された号の論文の9件中1～9を表示しています
- |<
- <
- 1
- >
- >|
-
笹野 伸昭1987 年 63 巻 3 号 p. 173-183
発行日: 1987/03/20
公開日: 2012/09/24
ジャーナル フリーRecent progress in research methodology particularly of immunocytochemistry has made the viaduct of human pathology through endocrinology a wide bridge. Immunohistochemical demonstration of enzymes working in corticosteroidogenesis is useful for the interpretation of histological findings. Cytochrome P-450c21 was demonstrated in three adrenocortical layers particularly evident in the glomerulosa and reticularis. The reactivity was intensive in hypertrophied cells in focal hyperplasia of autopsy series and adenomas in patients with hyperadrenocorticism. The distribution of extraadrenal steroid 21-hydroxylase was revealed by immunohistochemistry at the distal and collecting tubules of the kidney, excretory ducts of the pancreas and salivary glands, mammary ducts and ductules and secretory portion of the sweat gland in man. It was postulated that in these target tissues of the mineralocorticoid action considerable amounts of DOC would be produced from plasma progesterone by the extraadrenal 21-hydroxylase.
Histopathological diagnosis of malignancy in adrenal tumors has been extremely difficult. Therefore, follow-up informations of metastasis and/or recurrence and gross findings including the tumor weight have been evaluated as the evidence of malignancy.
In adrenocortical tumors plasma steroid patterns and in Vitro steroid production which were appreciated in the clinical endocrinology, were corroborated by gross and microscopical findings. Immunohistochemical lectin bindings revealed that only RCA (Ricinus communis agglutinin) might be useful for the marker of adrenocortical malignancies. For establishing histological criteria of malignant pheochromocytomas, 25 adrenal and extraadrenal tumors associated with metastases were examined. Localized or diffuse proliferation of small-sized cells, fusiform or round in shape, frequently associated with mitotic figures and foci of necrosis was of common feature. Immunoreactive peptide hormones were less compared with benign pheochromocytomas.抄録全体を表示PDF形式でダウンロード (3134K) -
三木 京子, 高松 順太, 坂根 貞樹, 馬嶋 素子, 北岡 治子, 北沢 明人, 茂在 敏司, 片山 正一, 森田 新二, 小林 彰, 松 ...1987 年 63 巻 3 号 p. 184-192
発行日: 1987/03/20
公開日: 2012/09/24
ジャーナル フリーPathology of the thyroid gland tissue was characterized in patients with Triiodothyronine (T3) -predominant Graves' disease who had normal levels of serum T4 but in-creased levels of serum T3 during antithyroid drug therapy. In order to compare this group of patients with an usual type of Graves' patients, age, sex, dietary intake of iodide, duration of antithyroid therapy and the dose of thionamide drugs were matched between the two groups of patients. Thyroid tissue was obtained from subtotal thyroidectomy. In examining the histology of the thyroid gland, integration eye piece plate II with regularly arranged 100 lattice points was set on eye lens of a microscope. The number of crossing points which were projected on each 4 histological elements was counted in randomly selected 10 fields of vision in each patient's thyroid. Assuming that the every histological element is arranged in a random manner, errors of each visual field by this method would be less than 12%. In consequence, the significant difference was demonstrated in the weight of subtotally resected thyroid and the ratio of each histological element except interstitial tissue occupied in the total thyroid tissue between T3-predominant Graves' disease and control Graves' disease. The volume composition of the epithelial cells and vacuoles in patients with T3 -predominant Graves' disease was significantly greater than that of patients with control Graves' disease (24.7±10.6 vs 14.4±6.5%, 5.9±3.9 vs 2.0±1.0%, Mean-± SD, respectively, p<0.05). In contrast, the volume composition of colloid was significantly less in T3 - predominant Graves' disease than that in control Graves' disease (65.9±13.4 vs 80.7±8.5%, p<0.05). The mean height of 50 epithelial cells examined in each patient was significantly higher in T3-predominant Graves' disease than in control Graves' disease (9.4±1.5 vs 6.7± 1.2g, p<0.001). The weight of the gland was greater in T3-predominant Graves' patients (95.4±39.0 vs 46.9±16.8g, p<0.01). In addition, the activity of serum TSH receptor anti-body was higher in T3 -predominant Graves' disease (60.5± 19.2 vs 22.6±17.7%, p<0.01).
Although iodide contents in thyroid were not measured in this study, our results indicate that the function of epithelial cell in T3 -predominant Graves' disease is more accentuated than that in control Graves' disease, which may be mediated by the markedly increased serum TSH receptor antibody activity in T3-predominant Graves' disease.抄録全体を表示PDF形式でダウンロード (1456K) -
松原 三八夫, 小田柿 栄之輔, 森岡 時世1987 年 63 巻 3 号 p. 193-197
発行日: 1987/03/20
公開日: 2012/09/24
ジャーナル フリーInhibition of plasma GH by dopaminergic agonists is one of the characteristics of the GH secretion in acromegaly. GRF is known to stimulate GH secretion in most patients with acromegaly. In order to elucidate the relationship between GRF and dopamine in regulating the secretion of GH in this disease, we examined plasma GH responses to dopamine (DA) infusion (4μEg/kg/min), GRF injection (100μEg i.v.), sulpiride (SP) injection (200mg i.v.), a DA blocker, DA plus GRF and SP plus GRF in a 51-year-old male patient with acromegaly.
Plasma GH was reduced to 14% of the initial level by iv infusion of DA, and was elevated to 158% by iv injection of GRF. No considerable change was observed in plasma GH by iv infusion of SP (114% of the initial level). GH release induced by GRF was remarkably reduced by simultaneous administration of DA (28% of the initial level), whereas SP administration did not affect GRF-induced GH release (154%). The marked reduction of GH release after DA plus GRF seems to suggest that the effect of DA on the GH regulation is stronger than that of GRF in this acromegalic patient. It is suggested also that endogenous DA may not play an inhibitory role in GH secretion in this case since DA blockade by SP did not raise basal GH levels and the GH response to GRF.抄録全体を表示PDF形式でダウンロード (407K) -
とくに3, 5, 3'-L-triiodothyronineとinsulinについて老籾 宗忠, 吉村 幸男, 川崎 富泰, 畑中 裕司, 八十 新治, 横野 浩一, 馬場 茂明, 武富 滋久1987 年 63 巻 3 号 p. 198-204
発行日: 1987/03/20
公開日: 2012/09/24
ジャーナル フリーIt has been demonstrated that the binding of cyanic acid, formed from urea which is increased in renal failure, to hemoglobin (Hb) results in the formation of HbAi. The combination of Hb and cyanic acid is called carbamylation, a nonspecific reaction between protein and cyanic acid. It is theoretically considered that like glycation, carbamylation may occur between cyanic acid and not only Hb but also various peptides and proteins. In the present study, hormones were subjected to carbamylation, and the effects of carbamylation on the biological activity of the hormones, i.e. 3, 5, 3'-L-triiodothyronine (T3) and insulin, were investigated. Carbamylated T3 and carbamylated insulin were synthesized in Vitro, using KCNO. The carbamylated hormones were isolated by high-performance liquid chromatography (HPLC). The biological activities of carbamylated T3 and carbamylated insulin were assayed by employing the metamorphosis of tadpoles (Rana nigrornaculata) and the glucose oxidation of fat cells or the receptor binding capacity of rat hepatocytes, respectively. The biological activity of carbamylated T3 was less than about 1/13 of that of T3, and the binding activity of carbamylated insulin was 1/5 of that of insulin. Therefore, carbamylation of hormones may be an important factor in the analysis of clinical conditions, especially the endocrine condition in renal failure.抄録全体を表示PDF形式でダウンロード (583K) -
第4報 マイクロプレートを用いる乾燥濾紙血液Cortisolの酵素免疫測定法の開発と新生児マス・スクリーニングへの応用福士 勝, 荒井 修, 水嶋 好清, 高杉 信男, 藤枝 憲二, 松浦 信夫1987 年 63 巻 3 号 p. 205-214
発行日: 1987/03/20
公開日: 2012/09/24
ジャーナル フリーAn enzyme-linked immunosorbent assay (ELISA) for Cortisol in dried blood collected on filter paper has been developed. The ELISA method is very simple and rapid and has sensitivity, accuracy and precision. The detection limit of the ELISA is 10ng/ml blood. The intra- and interassay coefficients of variation are 11.5±13.5% and 15.7- 16.7%, respectively.
Using the ELISA for Cortisol and 17-hydroxyprogesterone (17-OHP) previously reported, dried blood from normal newborns including premature infants (n=1583) and 21- hydroxylase deficiency (21-OHD) patient (n=9) were analyzed. 17-OHP, Cortisol and 17- OHP/Cortisol ratio of normal newborns are 12.5 ± 7.5ng/ml (1.0 - 140ng/ml), 89.0 ± 64.3 ng/ml (10 -1580ng/ml) and 0.16 ± 0.08 (0.01 - 0.86), respectively. In 21-OHD patients, 17-OHP is 109 -1361ng/ml, Cortisol is 35.1 - 146.7ng/ml and 17-OHP/cortisol ratio is 1.84 - 12.2. It made recall rate less to measure Cortisol in addition to 17-OHP and to take 17-OHP/Cortisol ratio.
Therefore, additional measurement of Cortisol to the primary 17-OHP screening test is advantaged in differentiating the normal newborns from the 21-OHD patients.抄録全体を表示PDF形式でダウンロード (877K) -
久保田 俊郎, 長江 光芳, 帝威 安遜, 斎藤 幹1987 年 63 巻 3 号 p. 215-226
発行日: 1987/03/20
公開日: 2012/09/24
ジャーナル フリーThe effects of a dopamine antagonist and stress of labor on prolactin (PRL) concentrations were studied in maternal plasma and amniotic fluid during term delivery.
In procedure 1, maternal blood and amniotic fluid samples were obtained from 5 normal full-term deliveries at 20-minute intervals for 180 minutes after the intravenous bolus of 10mg metoclopramide (MCP) during labor just before delivery. Amniotic fluid samples were drawn through a transcervical intrauterine pressure catheter to avoid contamination with maternal blood. PRL, 17β-estradiol (E2), progesterone (P) and cortisol were measured by means of radioimmunoassay (RIA), and free MCP concentrations were measured by high pressure liquid chromatography.
In procedure 2, maternal blood and amniotic fluid samples were obtained from 12 full-term pregnancies with spontaneous delivery and 10 cases of elective cesarean section when birth was imminent. The amniotic fluid was obtained by direct sampling from the forewaters in spontaneous delivery and by means of a syringe inserted through amniotic membrane after the uterine wall had been incised in cesarean section. PRL, β-endorphin (β-EP) and cortisol were measured by RIA.
The PRL levels in maternal palsma increased significantly (p<0.01) after the MCP injection. The peak value of PRL net increase (ΔPRL) was 676.5 ± 189.6 ng/ml. However, the PRL levels in amniotic fluid did not change significantly after the administration of this drug. Although the APRL levels in maternal plasma were significantly (p<;0.001) correlated with MCP concentrations (r = 0.812) after the MCP injection, there was no correlation between APRL and MCP concentrations in amniotic fluid. No significant changes in E2, P and cortisol levels in both samples were observed after the MCP injection.
The plasma PRL levels in vaginal delivery cases were significantly (p<0.05) lower than those in elective cesarean section cases (123.3 ± 15.8ng/ml vs. 181.0 ± 22.2ng/ml), and the plasma 0-EP and cortisol levels in vaginal delivery cases were significantly (p<0.01, p<0.001) higher than those in elective cesarean section cases respectively (13-EP : 134.8 ± 25.6pg/ml vs. 49.7 ± 12.3pg/ml, cortisol : 79.4 ± 5.8pg/d1 vs. 30.2 ± 3.3pg/dl). Therefore, a significant reduction in plasma PRL levels was accompanied by a marked rise in plasma 13-EP and cortisol levels during labor. In amniotic fluid, however, there were no significant differences of PRL, β-EP and cortisol levels in vaginal delivery cases and elective cesarean cases.
From these results, the authors conclude that the PRL-releasing system in amniotic fluid is independent of the maternal hypothalamo-pituitary axis, and that the stress of labor gives significant effect on PRL, β-EP and cortisol levels in maternal plasma, but no effect on these hormones in amniotic fluid just before delivery.抄録全体を表示PDF形式でダウンロード (1067K) -
佐藤 和宏1987 年 63 巻 3 号 p. 227-238
発行日: 1987/03/20
公開日: 2012/09/24
ジャーナル フリー17α, 20α-dihydroxy-4-pregnen-3-one (17α, 20α-P) is reportedly a hyper-tensinogenic substance in a model of ACTH induced hypertension in sheep (Scoggins et al). This steroid has not previously been measured in Japan. We have developed a new radioimmunoassay (RIA) system for 17α, 20α-P, and measured this steroid in peripheral blood samples from non-pregnant and pregnant women. In this clinical investigation concentrations of 17α-hydroxyprogesterone (17α-OHP) and progesterone (P) were also measured in the same clinical samples. Specific anti-serum against 17α, 20α-P-3-carboxymethyloxime-BSA was generated in rabbits. Cross-reactions with 5-pregnen-313, 17α 20α-triol (17α, 20α-P5) and 17α-OHP were 42 and 2.4%, respectively. Other steroids showed cross-reactivity of less than 1%. As an internal standard dexamethasone (500ng/100μl) was added to the samples which were extracted twice with 6 volumes of dichloromethane. Separation of the plasma extract was performed by high performance liquid chromatography (HPLC) with the ODS column in the solvent system, acetonitrile : water = 55 : 45. Eluates of the 17α, 20α-P and 17α-OHP fractions were collected separately and the solvent was evaporated in vacuo. RIA was carried out on each extract with specific anti-sera. RIA of P was performed independent-ly using the anti-P-3-CMO-BSA antiserum; 0.1 ml of serum was extracted with n-hexane and subjected to RIA without chromatography. The coefficient of variation for intra- and inter-assay (n = 10) were 8.5 and 11.7% for 17α, 20α-P; 7.2 and 12.1% for 17-OHP and 10.5 and 14.0% for P, respectively. The recovery rates of 17α, 20α-P, 17α-OHP and P were 95 ± 1.5, 93.2 ± 3.5 and 97.5 ± 4.2%, respectively. With the use of this method, 17α, 20α-P was measured in the blood samples from the menstrual cycle and normal pregnancy. The mean serum 17α, 20α-P concentrations ± SD in the follicular, ovulatory and luteal phases, and in the first, second and third trimesters were; 76 ± 6.0,100 ± 31,101 ± 6.4,226 ± 80,151 ± 48 and 367 ± 99pg/ml, respectively. While the concentrations of 17α-OHP and P in the respective timings were; 0.92 ± 0.5 and 5.3 ± 1.2, 1.8 ± 0.3 and 13.5 ± 4.2, 1.6 ± 0.6 and 68.7 ± 28, and 5.2 ± 1.1 and 128 ± 46ng/ml. The normal women in the ovulatory and luteal phase showed slightly higher levels of 17α, 20α-P as compared to the value in the follicular phase. Comparing to the values of steroid concentrations in the luteal phase, all the steroids increased significantly during pregnancy. Concentrations of all steroids in the third trimester were significantly elevated as compared to those in the first trimester of pregnancy. 17α, 20α-P and 17α-OHP showed a similar tendency to decrease in the second trimester and they increased again in the third trimester. The source of 17α, 20α-P in human is not entirely clear yet, but it may be postulated that this steroid is produced in the ovary and the fetoplacental unit.抄録全体を表示PDF形式でダウンロード (1010K) -
補充前後の検討安田 圭吾, 武田 則之, 堀谷 登美子, 今井 龍幸, 山田 浩司, 林 愼, 後藤 忍, 青山 かおり, 三浦 清, 長井 孝太郎, 棚 ...1987 年 63 巻 3 号 p. 239-246
発行日: 1987/03/20
公開日: 2012/09/24
ジャーナル フリーThe effect of chronic glucocorticoid deficiency on insulin binding to erythrocyte was evaluated in 2 cases of ACTH deficiency, 2 of Sheehan's syndrome and a case of Addison's disease before and after the replacement therapy with cortisone acetate. 75 gram oral glucose tolerance test (OGTT) was also performed. Mean fasting plasma glucose (FPG) and insulin (FIRI) before (70.2mg/d1, 3.6μEU/ml) and after (75.4 mg/dl, 7.8μEU/ml) therapy were significantly lower than those in normal control (97.0mg/d1, 12.0μU/ml). The ratio of FIRI/FPG before and after treatment were also significantly lower than that in control subjects. Glucose areas during OGTT before and after treatment were not different from that in control subjects. However, insulin area before treatment was significantly lower than that in control group. There were significant increases in FIRI/FPG and insulin area, but not in FPG and glucose area before and after treatment.
Insulin binding to erythrocytes before treatment (11.9 ± 1.1%, mean ± SD) was greater than that in normal subjects (7.7 ± 1.9%, n=19, p<0.05). It was significantly decreased and normalized to 7.8 ± 2.0% by the treatment. Analysis of binding parameters revealed the increases in receptor concentration at high affinity site (R1) and in average affinity at empty site by average affinity profile (Ke) before treatment in comparison with control subjects. There was no significant difference in binding parameters after treatment and in control group. High R1 or low R2 observed before treatment was significantly decreased or increased after treatment, respectively.
These results suggest that increase in insulin sensitivity in chronic glucocorticoid deficiency may be partly related to the increase in insulin binding to the receptor.抄録全体を表示PDF形式でダウンロード (778K) -
黒田 譲治1987 年 63 巻 3 号 p. 247-259
発行日: 1987/03/20
公開日: 2012/09/24
ジャーナル フリーVarious treatments have been applied to polycystic ovarian (PCO) type of anovulation. However, none of them was definitive in terms of the efficacy and side effects. Six anovulatory women of PCO type were treated with pulsatile gonadotropin-releasing hormone (GnRH) of various pulse intervals and continuous human menopausal gonadotropin (hMG). The efficacy and rationale of the treatments were discussed.
The subjects were diagnosed PCO by GnRH test and/or laparoscopy. They did not ovulate with clomiphene, clomiphene-hCG and hMG-hCG therapies. Their pretreatment serum FSH and LH levels and FSH/LH ratios were 6.9 ± 1.2mIU/ml, 15.7 ± 5.1mIU/ml, and 0.54 ± 0.19 (Mean ± SD), respectively. The treatment consisted of 3 protocols : 1) pulsatile GnRH (5-10μg/pulse) of 90 min interval, 2) pulsatile GnRH (5-10/μg/pulse) of 120 min interval and 3) continuous hMG (1501U/day) through subcutaneous route. Follicular growth was monitored sonographically and an intramuscular bolus of 10,000 IU hCG was given when the dominant follicle reached 20mm in diameter. During both GnRH treatments serum FSH levels and FSH/LH ratios did not elevate substantially. Serum LH, E2 and PRL levels elevated acutely and transiently during the initial phase of GnRH treatments. Follicular growth was observed in a small fraction of the cases, but none of them ovulated. In contrast, continuous hMG treatment induced significant elevation in serum FSH levels (8.2 ± 1.7 mIU/ml; p<0.01) and FSH/LH ratios (1.73 ± 0.57; p<0.001). Transient hyperprolactinemia was accompanied with the preovulatory E2 rise. All the cases ovulated and 3 singleton pregnancies followed.These findings draw conclusions as follows.
1) Pulsatile GnRH administration may desensitize the pituitary presumably due to increased GnRH pulse frequency as a consequence of two independent pulse generators, intrinsic and exogeneous. 2) It may induce transient hyperprolactinemia through a paracrine system between gonadotrophs and lactotrophs. 3) As a due course pulsatile GnRH therapy is questionable for ovulation induction in cases with functioning hypothalamic-pituitary axis. 4) The fact that continuous hMG effectively induced follicle maturation with elevated FSH/LH ratios suggested that FSH dominance might be a prerequisite for folliculogenesis. 5) The fluctuating nature of gonadotropins might not be mandatory for folliculogenesis.抄録全体を表示PDF形式でダウンロード (1300K)
- |<
- <
- 1
- >
- >|