Kanzo
Online ISSN : 1881-3593
Print ISSN : 0451-4203
ISSN-L : 0451-4203
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Volume 24, Issue 2
Displaying 1-18 of 18 articles from this issue
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  • Immunohistochemical localization of epoxide hydrolase, γ-glutamyl transpeptidase and α-fetoprotein during hepatocarcinogenesis induced by 2-acetylaminofluorene
    Seishiro WATANABE, Mikio NISHIOKA, Kiwamu OKITA, Masa-aki OGINO, Makot ...
    1983 Volume 24 Issue 2 Pages 123-130
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.123
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    The localization of Epoxide hydrolase (EH), γ-glutamyl transpeptidase (γ-GPT) and α-fetoprotein (AFP) in the liver was investigated during the hepatocarcinogenesis.
    Immunohistochemical technique was used in order to detect these markers. The localization of EH in the liver was almost the same as that of γ-GTP during the course of experiment. Many hyperplastic nodules were uniformly stained with EH and γ-GTP. However, AFP was shown to be different from the two enzymes. AFP positive cells were distributed ununiformly in the hyperplastic nodule. EH, γ-GTP and AFP were maintained in high level in the hyperplastic nodule and they were also identified in the hepatocellular carcinama.
    So far as these three markers were concerned, the alteration of the phenotypic expression was augmented in the hyperplastic nodule during the course of experiment. In conclusion, the hyperplastic nodule seems to be an important preneoplastic lesion.
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  • Enzyme histochemical classification of hepatocellular carcinoma according to gamma glutamyl transpeptidase activity
    The relation between histological type and serum γ-GTP value
    Mitsugu TANAKA, Junko UMIHARA, Keizo TAKAKI, Tadakazu SHIMODA, Eisei I ...
    1983 Volume 24 Issue 2 Pages 131-137
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.131
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    Eighty-six cases of hepatocellular carcinoma were classified into 4 types according to the γ-GTP activity, and a comparative study was carried out between the histochemical types and the serum γ-GTP value of hepatocellular carcinoma cases.
    Type I (fetal type), 36 of 86 cases (41.9%), indicating marked γ-GPT activity on not only the cell membrane but also in the cytoplasm of almost all tumor cells similating fetal liver and Type II (adult type), 16 of 86 cases (18.6%), showing γ-GPT activity along the tumor cell membrane coinciding to the bile canaliculi were similar to Grade II of Edmondson's classification.
    Type III (mixed type), 21 of 86 cases (24.4%), indicating different degree of γ-GPT activity in varying sites was similar to Grade III of Edmondson's classification.
    Type IV (embryonal type), 13 of 86 cases (15.1%), showing hardly any activity for γ-GPT was similar to Grade IV of Edmondson's calssification.
    Each type classified by γ-GPT activity demonstrated a correlation with serum value.
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  • Studies on the pathophysiology of acute hepatic failure (3) pathogenesis and pathophysiology of acute renal failure in galactosamine-induced fulminant hepatitis of rabbits
    Takuji YAMADA, Hiroshi FUKUI, Hidetsugu ITO, Masahito UEMURA, Masahiko ...
    1983 Volume 24 Issue 2 Pages 138-148
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.138
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    The pathogenesis and pathophysiology of renal failure were investigated in rabbits with fulminant hepatic failure induced by intravenous administration of galactosamine hydrochloride. Most rabbits developed disseminated intravascular coagulation (DIC) and acute renal failure following a typical course of fulminat hepatic failure. At 24hr corticomedullary shift of renal circulation coincident with functional renal failure was observed. It was suggested that functional renal failure may finally progress to acute tubular necrosis. Tubular changes were more remarkable in animals with glomerular microclots. The incidence of endotoxemia reached 90% and renal cortical blood flow in animals with severe endotoxemia was significantly lower than in those with mild endotoxemia. However renal blood flow. during endotoxin infusion showed variable changes associated with coagulation system. It is concluded that endotoxemia and concomitant DIC may play an important role in the development of acute renal failure during the course of fulminant hepatic failure.
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  • Immunohistochemical investigation of hepatitis B virus (HBV) associated antigens in liver tissues in asymptomatic HBs antigen carrier associated with simultaneous hepatitis A virus (HAV) infection
    Kiyohiro HIGUCHI, Takashi KOJIMA, Keiichi AOYAMA, Shinjiro MATSUI, Tos ...
    1983 Volume 24 Issue 2 Pages 149-160
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.149
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    In three asymptomatic HBs antigen carriers infected with HAV the changes of serum HBV associated antigen and antibody system and HBV associated antigens in liver tissue were investigated.
    1) In serum, HBs antigen titer was low in acute phase of superimposed type A viral hepatitis and increased in recovery phase. Anti-HBc titer showed no similar changes. HBe antigen and anti-HBe revealed almost the same level throughout the course.
    2) In liver tissue, HBs antigen positive hepatocyte was rare or disappeared in acute phase of superimposed type A viral hepatitis and increased distinctly in recovery and healing phase. One case revealed marked membranous localization of HBs antigen in recovery phase. HBc and HBe antigens were rare in acute phase and increased in healing phase in serum HBe antigen positive case.
    3) It is suggested that the changes of HBs antigen titer in serum reflected amounts of HBs antigen produced in liver tissue. However, changes of serum HBe antigen titer and amount of HBe antigen in hepatocytes showed no pararell relation.
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  • Diagnostic and prognostic significance of portal changes in acute viral hepatitis
    Tadao OKUNO, Atsushi SANO, Takeshi DEGUCHI, Yoshinori KATSUMA, Toshiak ...
    1983 Volume 24 Issue 2 Pages 161-166
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.161
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    Of 100 patients with clinical and biochemical characteristics consistent with acute viral hepatitis, all of whom were biopsied, 32 patients (32%) showed the disproportionate enlargement of portal tracts with moderate to severe inflammatory cell infiltration. At the same time, the lobular parenchyma exhibited features of acute hepatitis. These 32 patients were included 5 hepatitis A, 14 hepatitis B and 13 non-A, non-B hepatitis. The mean age of them was 40.7 years.
    All patients with acute hepatitis A and B as well as 10 of 13 patients with acute non-A, non-B hepatitis made a full recovery in spite of histological "chronicity".
    However, in 3 patients with non-A, non-B hepatitis, serum transaminases remained abnormal for more than 6 months. In 2 patients, follow-up biopsies were carried out and the histological diagnosis of CAH was obtained. The prognosis of acute viral hepatitis appeared to be related to the etiological factors rather than the histological features.
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  • Apoprotein E-rich high density lipoprotein in liver disease and cholestasis
    Yasuji MIYATA, Shunichi KOGA, Hiroshi IBAYASHI
    1983 Volume 24 Issue 2 Pages 167-173
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.167
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    Content of apoprotein E (apo E) in high density lipoprotein (HDL) was examined in hepatobiliary disease by semiquantitative method of SDS polyacrylamide gel electrophoresis.
    Abnormal increase of HDL apo E was observed in 10 of 20 with acute hepatitis, 2 of 5 with hepatocellular carcinoma and 2 of 3 with obstructive jaundice. Manifest HDL apo E was observed in patients with severe form of acute hepatitis ans in terminal stage of hepatocellular carcinoma, which was accompanied with marked decrease of HDL, hyperbilirubinemia and the elevation of alkaline phosphatase activity.
    Apo E content in HDL from patients with chronic hepatitis and compensated cirrhosis were almost normal. The appearance of apo E-rich high density lipoprotein in patients with severe liver disease and cholestasis suggests abnormalities of nascent HDL metabolism due to decreased LCAT activity. But mechanisms which may contribute appearance of apo E-rich HDL in patients with cholestasis need farther investigation.
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  • Serum lipoprotein profiles and its structural changes isolated by isopycnic density gradient ultracentrifugation in liver cirrhosis
    Susumu YUKAWA, Keiji MIMURA, Masahiro KINOSHITA, Takao MAEDA, Kazuo MO ...
    1983 Volume 24 Issue 2 Pages 174-181
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.174
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    To investigate serum lipoprotein abnormalities in liver cirrhosis, we have employed isopycnic density gradient ultracentrifugation to separate the lipoproteins (d. 1.019-1.210g/ml) into its subclasses. The results were as follows.
    1) In the lipoprotein profiles, low density lipoproteins (LDL) and high density lipoproteins (HDL)2 in liver cirrhosis shifted to lower density than those of normal controls.
    2) Patients with liver cirrhosis had a significant increase in triglycerides in all of lipoprotein subclasses and in phospholipids in HDL and a reciprocal change about protein in HDL. In addtion, a significant decrease in cholesteryl ester in LDL and HDL2, particularly in LDL, was observed,
    3) In cirrhotics, there was a significant increase in HDL2-Apo A-I/A-II ratio which was same as in serum.
    4) HDL2 in liver cirrhosis possessed small amounts of Apo B which were usually undetectable in normal controls.
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  • Studies on hepatocarcinogenesis by oral contraceptive, synthetic estrogen and progestogen. Sequential development of hyperplastic nodules and hepatocellular carcinomas and effect of withdrawal of hormone administration on the developed hyperplastic nodules
    Takazumi IMAI
    1983 Volume 24 Issue 2 Pages 182-192
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.182
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    The purpose of this study was to investigate the hepatocarcinogenicity of the long term administration of oral contraceptives. Female rats of the Wistar strain aged 4 weeks were given a dose of 0.075mg of ethynyl setradiol and 6mg of norethindrone acetate dissolved in 0.5ml of olive oil daily through a gastric tube without anesthesia for one year and sacrificed every two months.
    Two months later, liver cells in periportal area showed an increase of γ-GTPase reaction without hyperplastic foci or nodules. Four months later, however, hyperplastic nodules were observed in all hormone-treated rats. Number and area of the hyperplastic nodules increased progressively during continuous administration of oral contraceptives.
    A hepatocellular carcinoma developed in one (10%) of 10 rats at 8 months following start of the hormone administration, and also in one (10%) of ten at 10 months, and in one (5.6%) of eithteen at 12 months, respectively. On the other hand, no neoplastic lesions were observed in the other organs of hormone-treated rats. Neither hyperplastic nodules in the liver nor neoplastic lesions in any organs developed in the control rats.
    After withdrawal of oral contraceptives, hyperplastic nodules disappeared gradually.
    These results suggest that oral contraceptives, synthetic estrogen and progestogen, might initiate hepatocarcinogenesis and be one of carcinogens.
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  • An autopsy case of pleomorphic hepatocellular carcinoma with a huge metastatic mass in the great omentum
    Tadashi NAKAMURA, Kemmei SAKATA, Tetsuro YAMAMOTO, Keishi UEDA, Takesh ...
    1983 Volume 24 Issue 2 Pages 193-200
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.193
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    An autopsy case of 73-year-old male of hepatocellular carcinoma with its huge metastatic omental mass was reported.
    Celiac arteriography disclosed a huge hypervascular tumor supplied by gastroepiploic artery. Histopathologically it was suspected to be a malignant fibrous histiocytoma. However, the mass contained epithelial-like elements and matastatic cancer was undeniable.
    Autopsy revealed the liver with many nodular masses in size from millet to fist, and the liver had no cirrhotic patterns. Topographical histopathological study of the liver showed them to be hepatocellular carcinoma (pleomorphic variant) with sarcomatous proliferation of the cells. The omental mass was a metastasis of the hepatocellular carcinoma.
    A huge omental mass is a rare disease but hepatocellular carcinoma is a common problem. The relationship between them is discussed.
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  • A case of hepatocellular carcinoma presenting with Jacksonian seizures as initial symptom
    Shinichi HINO, Masahiko IGARASHI, Motohide TAKASHI, Hiroshi HORIE, Tos ...
    1983 Volume 24 Issue 2 Pages 201-207
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.201
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    A 58-year-old man with hepatocellular carcinoma who first presented with Jacksonian seizures and died form progressive intracranial complications is described. Autopsy revealed several small lesions of hepatocellular carcinoma with diffuse dissemination of small secondaries throughout the liver, and metastases to various organs and tissues. The cause of the epileptic attacks was thought to be compression upon the right motor area of the cortex and infiltration by the tumor metastasizing to the calvaria. Judging from the content and maturity of collagen fibers in the involved cerebrum, meninges, calvaria and epicranial aponeurosis, it was assumed that the primary matastasis had occurred to the bone-marrow of the calvaria. The discussion includes a review of the world literature on bone metastasis of hepatocellular carcinoma and statistical analyses of 4, 525 hepatocellular carcinoma cases registered in the Annals of Pathological Autopsy Cases in Japan for 1978, 1979 and 1980.
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  • A case of hepatocellular carcinoma treated with human fibroblast interferon (HuIFN-β)
    Yuro SHIBAYAMA, Kazuki MATSUMOTO, Gen OHI, Katsuji NAKATA, Kohichi KAK ...
    1983 Volume 24 Issue 2 Pages 208-216
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.208
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    Human fibroblast interferon (HulFN-β) was administered by the intramuscular (total dose, 4671×104 international reference units, IU), intravenous (total dose, 2866×104IU), and intraarterial (total dose, 250×104 IU) routes in a 62-year-old man with hepatocellular carcinoma for 3 months. On admission, the mass of hepatocellular carcinoma was already recognized at all parts of the right lobe and a part of the left lobe and marked elevation of α-Fetoprotein level. The α-Fetoprotein level was decreased by the direct intrahepatic injection of HuIFN-β by the intraarterial route, but the size of the tumor wasgradually increased. No remarkable change was recognized by the intramuscular or intravenous ad-ministration. Only fever and the following subjective and objective symptoms were recognized as the side-effects of HulNF-β administration. Multinucleated giant cell transformation and a lack of a tendency to necrosis of the cancer cells were histopathologically recognized at the autopsy. These findings were, however, seen in some cases of hepatocellular carcinoma without the administration of HuIFN-β. Accordingly, we cannot but say that the therapeutic effect of HulFN-β on hepatocellular carcinoma are obscure in at least the present case.
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1983 Volume 24 Issue 2 Pages 217
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.217
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1983 Volume 24 Issue 2 Pages 218
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.218
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese]
    1983 Volume 24 Issue 2 Pages 219
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.219
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1983 Volume 24 Issue 2 Pages 220
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.220
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1983 Volume 24 Issue 2 Pages 221
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.221
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1983 Volume 24 Issue 2 Pages 222
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.222
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  • [title in Japanese]
    1983 Volume 24 Issue 2 Pages 223-259
    Published: February 25, 1983
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.24.223
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