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51 巻, 1 号
選択された号の論文の6件中1～6を表示しています
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桜田 俊郎, 吉田 克己, 山口 徹, 山本 蒔子, 斉藤 慎太郎, 福地 総逸, 吉永 馨1975 年 51 巻 1 号 p. 1-8
発行日: 1975/01/20
公開日: 2012/09/24
ジャーナル フリーEffect of l-methyl-2-mercaptoimidazole (MMI) on the serum triiodothyronine (T3) and thyroxine (T4) was studied in six patients with hyperthyroidism. From 30 to 60 mg of MMI per day were administered to the patients orally. Serum T3 was measured by radioimmunoassay and T4 by radioimmunoassay or Tetrasorb Kit (Abbott Lab.).
Serum T4/T3 ratios in these patients were low initially, but increased to normal range in two cases immediately after the administration of MMI. But they were unchanged at first in the other four cases. When high serum T3 values decreased abruptly to normal at a later course of mediaction, the serum T4/T3 ratios increased to normal range in almost all cases. Even though the serum T4/T3 ratio became normal, serum T3 and T4 values remained high in some cases.
Elevated serum T4 values in all cases decreased to normal faster than serum T3 with the administration of MMI.
When MMI was administered to the patients continuously, the T4/T3 ratios, once increased to normal, then decreased again gradually in five cases. And serum T3 which decreased to normal sometimes gave high values.
After the discontinuation of MMI, serum T3 increased over the normal range more rapidly than did T4.抄録全体を表示PDF形式でダウンロード (867K) -
下田 新一, 竹村 喜弘, 家入 蒼生夫, 菊地 敏徳, 笠井 貴久男, 殿岡 伸彦, 小林 正己, 関 健鑑1975 年 51 巻 1 号 p. 9-17
発行日: 1975/01/20
公開日: 2012/09/24
ジャーナル フリーRecently, L-dopa has been known as one of drugs to stimulate the secretion of growth hormone from the pituitary gland through dopamine, which is a metabolite of L-dopa, without any changes of serum insulin levels, blood sugar values, fatty acids and amino acids concentrations in the serum of human being. The present study was thus designed to assess the effect of L-dopa on the pituitary gland to secrete the growth hormone in the normal subject and the study was further extended to compare the response of the pituitary secretion of the growth hormone following the administration of L-dopa between in the patient with diabetes mellitus and in the normal subject.
In the present experiment, 5 normal subjects and 11 diabetes mellitus without obesity were employed and they received a 30 min infusion of 200 mg L-dopa dissolved in 200 ml physiological saline. In normal subjects, serum growth hormone concentration measured by radio-immunoassay was started to increase within 10 min after L-dopa administration and maximum value of serum growth hormone was obtained at 60 min after the drug, mean values of it, 30 ng/ml of serum. Then it was declined sharply upto the values of 2-3 ng/ml. In the patients with diabetes mellitus, on the other hand, maximum value of serum concentration of the growth hormone was only approximately 5 ng/ml of serum obtained between 45 and 75 min after the administration of L-dopa. No changes in the serum concentration of IRI (Immunoreactive insulin) and of blood sugar values were observed by the infusion of L-dopa in both noraml subjects and diabetes mellitus. From the above mentioned facts, it was concluded that the ability of pituitary gland to secrete the growth hormone was considerably impaired in diabetics when it was compared with normal subjects.抄録全体を表示PDF形式でダウンロード (788K) -
第1篇 固相法ラジオイムノアッセイ法による血清TSH測定法ならびにその臨床応用竹田 洋祐1975 年 51 巻 1 号 p. 18-35
発行日: 1975/01/20
公開日: 2012/09/24
ジャーナル フリーA solid-state RIA method using a plastic microtiter plate for human TSH was developed :
1) The choice of carrier protein for standard TSH was critical in this method and pooled sera from untreated Graves patients was found to be suitable for this purpose.
The mean lowest detectable TSH level was 0.2 μU/assay, which was almost equal to those reported by other methods. This method is superior in simple assay procedure, especially in the separation of bound and free TSH and in the shorter incubation time required in the double antibody method.
2) Serum TSH concentration in 22 normal subjects, 17 patients with Graves' disease, 35 Hashimoto's thyroiditis, 18 primary hypothyrodism, 16 simple goiter, 4 nodular goiter and 7 secondary hypothyroidism was estimated as 4.7±2.0 μU/ml (mean±s.d.), 2.1±0.2 μU/ml, 14.1±26.5 μU/ml, 211±177 μU/ml, 3.6±2.4 μU/ml, 3.2±2.4 μU/ml and 2.6±1.0 μU/ml, respectively.
3) A statistically significant and hyperbolic inverse correlation (r= -0.37, N= 90) was found between TSH and T4 levels. Some cases with normal T4 level were found to be high in TSB levels. It was also noted that 36 of 65 euthyroid cases (55.4%) who had been treated with 131I for Graves' disease showed elevated TSH levels.
4) After intravenous injection of 500 μg TRH, TSH level reached its peak value of 8 to 32 μU/ml at 15 to 45 minutes in normal subjects. Low to no response was found in patients with Graves' disease. An exaggerated response in patients with primary hypothyroidism to TRH was observed and an inhibitory process in TSH production at the pituitary level was suggested in patients with Cushing syndrome.
Hypothyroid patients with pituitary lesion showed low or no response, on the other hand some hypothyroid patients with lesions around the pituitary and hypothalamus showed high basal TSH and exaggerated response to TRH.抄録全体を表示PDF形式でダウンロード (1437K) -
第2篇 迅速ラジオイムノアッセイ法による血清T3測定法ならびにその臨床応用竹田 洋祐1975 年 51 巻 1 号 p. 36-47
発行日: 1975/01/20
公開日: 2012/09/24
ジャーナル フリーA rapid and simple radiommunoassay method for serum T3 was developed using home made potent rabbit anti-T3 serum :
1) The incubation mixture which contained 0.1 ml of 1 : 2,000 diluted anti-T3 serum (finally 1 : 20,000), 250 μg of 8-anilino-1-naphthalene sulphonic acid and 0.1 ml of serum was run at 37°C for 1 hour. Dextran-coated charcoal was added for separation of free and bound T3.
No significant undersirable effects due to high incubation temperature and short incubation time was observed. The lowest detectable amount of T3 was 20 pg per assay though this antiserum showed a 0.24% cross reaction with T4 the purity of which was 99%. ti The dilution curve of serum from Graves' patients was parallel to the standard. The recovery of added T4 over the range 39-625 pg was 98.1%. Inter and intraassay variations of the estimated T3 values at the euthyroid level were 14.3% and 16.9%, respectively.
2) Serum T3 concentrations in 21 normal subjects, 23 patients with Graves' disease, 9 primary hypothyroidism, 19 Hashimoto's thyroiditis were estimated as 131±29 ng/100 ml (mean±s.d.), 546±274 ng/100 ml, 77±27 ng/100 ml and 154±30 ng/100 ml respectively. Calculated T3/T4% in 10 normal subjects was 1.48±0.44, in 21 Graves' patients 2.84±1.59, in 9 primary hypothyroidism 3.88±1.36 and 19 Hashimoto's thyroiditis 2.41±0.86.
3) T3 levels in 131I treated Graves' patients corresponded well with their thyroid functional status. On the other hand their T3/T4% was distributed over a wide range and had no significant relationship with the clinical status.
A hyperbolic inverse correlation between serum T3 and TSH levels (r=-0.21) was found in 88 cases with various thyroid disease, 25 cases of which showed high TSH with normal T3 levels.
A significant increase in serum T3 at 90 and 120 minutes after intravenous administration of 500 μg TRH was observed, and the average of the absolute maximum increment was 42±24 ng/100 ml in 9 normal subjects. It is considered feasible to evaluate both thyroid and pituitary reserve quantitatively by simultaneous estimation of serum T3 and TSH levels after TRH administration.抄録全体を表示PDF形式でダウンロード (1057K) -
加古 健1975 年 51 巻 1 号 p. 48-55
発行日: 1975/01/20
公開日: 2012/09/24
ジャーナル フリーIsolated adrenal cells were obtained surgically from patients with primary aldosteronism, breast cancer, or Cushing's syndrome. They were prepared by the modification of Sayers method, and incubated at 37°C for 2 hours under 95% O2-5% CO2, in the medium of calcium-free Krebs-Ringer bicarbonate buffer containing 0.2% glucose and 0.5% bovine serum albumin, to which various doses of calcium, ACTH, dibutyryl cyclic AMP or cycloheximide were added. Steroid production was measured by the method of Silber et al.
In isolated normal adrenocortical cells, 11-OHCS was produced by calcium alone in the absence of ACTH or dibutyryl cyclic AMP, while it was not produced by ACTH alone without calcium. 11-0HCS production by ACTH was decreased in the high concentration of calcium (10.16 mM, 12.70 mM). Cycloheximide partially blocked an increase in 11-OHCS synthesis induced by clacium. These data suggest that adenyl cyclase of human adrenocortical cells may be stimulated by calcium alone, supporting the notion that calcium is a second messenger.
The ratio of 11-OHCS production by calcium alone to that by dibutyryl cyclic AMP was higher in adenoma cells than in normal cells. This may account for the character of autonomic steroid production in adrenocortical adenoma cells.抄録全体を表示PDF形式でダウンロード (876K) -
Thyroxine誘導体d, 1-α-Methyl-3, 5, 3′, 5′-Tetraiodothyronine Ethylester Hydrochlorideの実験的動脈硬化症家兎におよぼす影響青沼 繁, 小浜 靖弘, 屋敷 伸治, 中陳 静男, 陳 英俊, 和田 昌師1975 年 51 巻 1 号 p. 56-67
発行日: 1975/01/20
公開日: 2012/09/24
ジャーナル フリーTo develop the prophylactics and the curatives for atherosclerosis, thyroxine derivative, CG-635, was assayed for its physiological activities in experimental atherosclerosis in rabbits fed with cholesterol. It was found that CG-635 possessed serum TC/TP value lowering activity (total cholesterol/total phospholipid) in normal and cholesterol fed rabbits for 3 weeks, and prevented the elevation of the value of cholesterol fed rabbits by daily injection for 7 weeks. CG-635 also depressed the hyperlipemia induced by cholesterol feeding, and its inhibitory effect was shown to be more marked on the increase of cholesterol than triglyceride, phospholipid and free fatty acid in serum. CG-635 did not, however, influence GOT, GPT and G-6-Pase activities in serum with increased cholesterol intake. From the histological findings it was proved that this compound prevented to a high degree the occurrence of atherosclerosis and fatty liver of cholesterol fed rabbits. Furthermore, it was recognized that thyroid hormone and the thyroid stimulating hormone-like activities of CG-635 were much weaker than thyroxine, except for the action in the lipid metabolism.抄録全体を表示PDF形式でダウンロード (4290K)
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