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55 巻, 2 号
選択された号の論文の8件中1～8を表示しています
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第3編 TRH test時のHGH分泌増戸 尚, 高田 一太郎, 見坊 隆, 渡辺 斌, 氷室 一彦, 稲垣 勝則1979 年 55 巻 2 号 p. 89-95
発行日: 1979/02/20
公開日: 2012/09/24
ジャーナル フリーHGH secretion in response to TRH was studied in patients with cerebrovascular disease in order to elucidate an influence of cerebrovascular lesions on the hypothalamus-anterior pituitary function.
Blood specimens were obtained before and at the time of 10, 20, 30, 40, 60, 90 and 120 minutes after the intravenous administration of 500 big of TRH in 17 patients with cerebral hemorrhage and in 8 patients with cerebral infarction.
With regards to the natural fluctuations of serum HGH caused by cerebrovascular disease, the blood specimens were obtained at the time of 0, 30, 60, 90 and 120 minutes without the administration of TRH in 4 patients with cerebral hemorrhage and in 4 patients with cerebral infarction.
Serum HGH was measured using the RIA method and the following results were obtained :
1) An increase in serum HGH was observed in 1 patient with cerebral hemorrhage out of 8 patients with cerebrovascular disease without TRH administration.
2) In 5 out of 17 patients with cerebral hemorrhage, an increase in serum HGH was observed following TRH administration. Three out of these 5 patient were female.
3) In 2 out of 8 patients with cerebral infarction, an increase in serum HGH was observed following TRH administration.抄録全体を表示PDF形式でダウンロード (587K) -
碓井 亜, 相模 浩二, 北野 太路, 仁平 寛巳, 宮地 幸隆, 平山 多秋1979 年 55 巻 2 号 p. 96-105
発行日: 1979/02/20
公開日: 2012/09/24
ジャーナル フリーTestosterone-estradiol binding globulin (TeBG) is known to change in various endocrinological environments such as estrogen administration, pregnancy and aging. Several methods, including dextran coated charcoal, equilibrium dialysis and ammonium sulfate precipitation, were used to measure the binding capacity of TeBG, but these were not simple and accurate. We therefore measured TeBG levels in human serum by means of a steady state polyacrylamide gel electrophoresis and found that this method was simple and accurate for the determination of the binding capacity of TeBG. The value of TeBG in normal adults (27-32 years old) was 3.88 ± 0.45 × 10-8Mol and in patients with benign prostatic hypertrophy the value was high (5.49 ± 1.35 × 10-8Mol compared to that of normal adults.抄録全体を表示PDF形式でダウンロード (640K) -
満間 照典, 野木森 剛, 広岡 良文1979 年 55 巻 2 号 p. 106-113
発行日: 1979/02/20
公開日: 2012/09/24
ジャーナル フリーThe effect of insulin administration on the hypothalamic-pituitary thyroid axis in the rat was studied. Regular insulin (1.0U/100g of body weight) was injected into male Wistar rats weighing 200g After decapitation, blood was collected at various intervals. TRH contents in the hypothalamus (H), and TRH, TSH, T4 and T3 levels in serum were measured by each specific radioimmunoassay. Blood glucose levels were measured by the autoanalyzer method.
Before the insulin injection, the TRH contents in H, TRH, TSH, T4 and T3 in serum were 4.2ng, 6pg/ml, 262ng/ml, 5.5μg/dl and 55ng/dl respectively with blood glucose 101mg/ dl. After the insulin injection, the TRH contents in H started to fall at 5min, reached its nadir at 20min, at which the level was 2.5ng, and then returned to initial levels. The TRH levels in serum rose to 47pg/ml at 5min and then returned to initial levels after the insulin injection. The TSH levels in serum increased at 15min, with a peak value (900ng/ml) at 30min after the insulin injection. The T3 levels in serum started to rise at 45min and reached 120ng/dl after the insulin injection. The T4 levels in serum did not change during the observation period.Blood glucose levels decreased rapidly, reached their nadir at 15min and then gradually returned to initial levels after the insulin injection. Pretreatment of 1.0g of glucose did not affect the TRH increase in serum after the insulin injection. Pretreatment of T4, T3 or dexamethasone suppressed the TRH increase in serum after the insulin injection.
These data suggest that insulin administration might stimulate TRH release from the hypothalamus and that T4, T3 or dexamethasone might inhibit TRH release in rats.抄録全体を表示PDF形式でダウンロード (677K) -
高橋 克幸, 菅原 愛子, 湊 敬一, 和田 裕一, 斉藤 晃, 古橋 信之, 桃野 耕太郎, 鈴木 雅洲1979 年 55 巻 2 号 p. 114-121
発行日: 1979/02/20
公開日: 2012/09/24
ジャーナル フリーFemale Wistar rats were divided into four groups.
Group 1 : The rats were pinealectomized by the Kuszak and Robin modified method or sham-operated 3 weeks after castration. They were injected with estrogen-progesterone 1 week after the pinealectomy and sacrificed by decapitation 48 hours after the injection.
Group 2 : The rats were pinealectomized or sham-operated after 1 week of pregnancy and decapitated after 20 days of pregnancy.
Group 3 : The rats were pinealectomized or sham-operated after 15-19 days of pregnancy. The litter was separated from the mother immediately after delivery. These non-lactating rats were sacrificed by decapitation 3 days after delivery.
Group 4 : Pinealectomy and sham-operation were done by the same method as in group 3. The litter was not separated and allowed to suckle. These lactating rats were decapitated 3 days after delivery.
Prolactin levels in the pituitaries and serum were determined by radioimmunoassay.
We found that pinealectomy made the serum prolactin level significantly increase in pregnant and puerperal rats, and significantly decrease in castrated rats. The hypophysial prolactin level decreased in all groups, but there was no significant difference apart from group 3.
These results indicate that the suckling stimulation made the prolactin secretion significantly increase even though the rats had undergone a pinealectomy. The pineal gland effects the hypothalamus appearing to inhibit PIF and PRF. This would suggest that the prolactin release action of the pineal gland has no relation to the route of the central nervous system concerning suckling stimulation.抄録全体を表示PDF形式でダウンロード (743K) -
内海 正文1979 年 55 巻 2 号 p. 122-132
発行日: 1979/02/20
公開日: 2012/09/24
ジャーナル フリーUsing a sensitive and specific radioimmunoassay for somatostatin (somatotropin release-inhibiting factor : SRIF), hypothalamic SRIF levels were investigated in Sprague Dawley rats.
1) The developmental patterns of hypothalamic immunoreactive SRIF (IR-SRIF) content and concentration were examined in normal rats (including females) until weaning, from the 4th day through the 70th day after birth. Animals were sacrificed by decapitation without anesthesia between 12 : 00 h and 14 : 00 h. The brains were immediately removed and frozen on dry-ice. Hypothalamic tissues were dissected out and extracted with 2.5 ml of 2 N acetic acid. Hypothalamic IR-SRIF contents were increased progressively from 1.87 ± 0.02 ng (Mean ± SE) on the 4th day to 3.01 ± 0.19 ng on the 7th day and 4.86 ± 0.34 ng on the 14th day of age, respectively. Thereafter the contents were elevated markedly to 10.5 ± 1.0 ng on the 22nd day of weaning and followed by a slow increment until the 36th day of age Coincidental with the pubertal period, IR-SRIF contents were again increased markedly tc 22.9 ± 0.3 ng on the 42nd day and reached the adult level of 27.4 ± 0.8 ng on the 46th day and of 27.3 ± 0.5 ng on the 70th day of age. As expressed in terms of pg IR-SRIF per mi wet weight, the developmental patterns of hypothalamic IR-SRIF concentrations appeared to be more distinctly biphasic than those of the contents.
2) The diurnal fluctuations of hypothalamic IR-SRIF levels were observed every 4 h over a 24 h period in adult intact male rats housed for two weeks in a controlled light (14 h of light starting at 05 : 30 h) and temperature (22 ± 2°C, 60 ± 5% of humidity) room and given free access to food and water. Hypothalamic IR-SRIF levels were increased from 22.6 ± 1.3 ng in content, 1016 ± 43 pg/mg wet weight in concentration at 20 : 00 h to 27.5 ± 0.4 ng, 1147 ± 41 pg/mg wet weight at 04 : 00 h and to a maximum of 28.5 ± 0.9 ng, 1217 ± 33 pg/ mg at 08 : 00 h, respectively, and followed by a gradual decline toward 12 : 00 h and 16 : 00. Furthermore, it is of interest that the hypothalamic levels of immunoreactive TRH fluctuated reciprocally in these rats.
3) A sex difference on hypothalamic IR-SRIF levels was examined. Hypothalamic IR-SRIF levels of male rats were not different from those of females of the same age (46 days) but slightly lower than those of females (53 days) of the same body weight.
4) The effect of food restriction for 24 h on hypothalamic IR-SRIF levels was studied in adult female rats. IR-SRIF levels in fasted rats were significantly reduced as compared with fed controls. (29.2 ± 0.5 ng vs 33.3 ± 0.9 ng in content, 1215 ± 47 pg/mg wet weight vs 1426 ± 53 pg/mg wet weight in concentration)
In conclusion, these observations indicate that hypothalamic IR-SRIF levels show apparent physiological fluctuations, especially on development, nyctohemeral cycle and food restriction in the rat. The possible roles of hypothalamic SRIF in this species are discussed.抄録全体を表示PDF形式でダウンロード (1055K) -
香山 浩二, 戸田 一司, 栗山 大作, 礒島 晋三1979 年 55 巻 2 号 p. 133-140
発行日: 1979/02/20
公開日: 2012/09/24
ジャーナル フリーMany results concerning the differences in hCG properties from normal pregnant women and trophoblastic tumor patients have been reported. In these, the serum and urinary hCG preparations were examined for electrophoretic mobility, ion exchange chromatography, amino acid and carbohydrate compositions, ratios of biologic activity to immunologic activity and others. In this paper, the affinity of urinary hCG to a lectin, lens culinaris hemagglutinin (LcH), was compared in normal pregnant women and in patients with hydatidiform mole and choriocarcinoma.
LcH was purified from lentil beans and coupled to CNBr activated sepharose 4B beads at the ratio of 5mg LcH to one ml of the gel so as to prepare the LcH coupled sepharose column (bed volume : 20ml). For a LcH affinity chromatography of the urinary hCG, 8ml of dialyzed urine was applied to the column and then fractionated. The LcH bound fractions were eluted with 2% mannoside solution. Each fraction was examined for hCG activity by radioimmunoassay, and the amounts of hCG in the LcH unbound fraction and the LcH bound fraction were calculated in each of the urine specimens.
In most of the pregnant women, more than 95% of hCG activity in the urine was eluted in the LcH bound fraction, whereas 8 to 26% and 37 to 40% of the activity was passed through in the LcH unbound fractions in hydatidiform mole and choriocarcinoma, respectively.
These results suggest that there are some differences in the carbohydrate moieties of urinary hCG structures in pregnant women and patients with hydatidiform mole and choriocarcinoma. Furthermore, these differences could possibly contribute to differentiating normal pregnancy from trophoblastic disease.抄録全体を表示PDF形式でダウンロード (731K) -
矢倉 俊洋, 内野 治人, 赤沢 好温, 東 淑江, 永田 格, 隈 寛二1979 年 55 巻 2 号 p. 141-152
発行日: 1979/02/20
公開日: 2012/09/24
ジャーナル フリーIt has been reported that cyclic nucleotide phosphodiesterase (PDE) has multiple forms, and one of them is activated by PDE-activating factor (PAF) in many tissues. Also it has been suggested that PAF plays an important role in the control of intracellular cyclic nucleotide concentrations. But PDE and PAF have not been studied sufficiently in the human thyroid. We report in this article the occurrence of multiple forms and of an activating factor of PDE in the human thyroid. We also report how the PDE activities and their properties in thyroids with various diseases differ from those in the normal thyroid.
The PDE activities for cyclic AMP and for cyclic GMP in the 100,000 × g supernatant of the human thyroid were activated by Ca2+ in the presence of Mg2+. Sephadex G-200 gel-filtration profiles of the activities of the supernatant revealed three peaks of cyclic AMP PDE activity, three peaks of cyclic GMP PDE activity and one peak of PAF activity. The second peaks of cyclic AMP and cyclic GMP PDE activities were eluted at the same position and were activated by PAF in the presence of Ca2+. The fact that the enzyme activities of other peaks were not influenced by Ca2+ indicated that Ca2+-dependency in the supernatant was due to the presence of the PAF-dependent PDE form. This activating system of PDE may have a role in the control of intracellular cyclic nucleotide concentrations in the human thyroid. The molecular weights of the PAF-dependent PDE form and PAF were estimated to be 145,000 and 26,000, respectively.
The PDE activities per gram of tissue for cyclic AMP and for cyclic GMP of the 100,000 × g supernatants of Graves' thyroid, thyroid adenoma and carcinoma were increased as compared with the normal thyroid. It could not be clarified whether or not this increase was merely due to cellular hypertrophy For an increase in cell population density. The ratios of cyclic GMP PDE activity to cyclic AMP PDE activity were increased in Graves' thyroid and thyroid carcinoma. Ca2+-Dependency of the PDE activity was increased in thyroid carcinoma when cyclic AMP was used as a substrate, and in Graves' thyroid and thyroid adenoma when cyclic GMP was used. These changes of the PDE activities suggest abnormality of cyclic nucleotide metabolism in the thyroids of patients with these diseases. Hydrolysis of cyclic AMP in Graves' thyroid may be influenced by altered PDE activity.抄録全体を表示PDF形式でダウンロード (1121K) -
川辺 勝弘, 常田 清, 神戸川 明, 大内 力男, 森 弘1979 年 55 巻 2 号 p. 153-160
発行日: 1979/02/20
公開日: 2012/09/24
ジャーナル フリーA quantitative analysis of seven components of 17-KS in human urine was successfully carried out by GC-MS. The sample including deuterated 17-KS was introduced into TMS derivatives by BSTFA in pyridine. The derived TMS derivatives were analysed using the GC-MS system equipped with the column of 0.5% NPGS Chromosorb W AW DMCS, and the temperature was programmed from 180°C to 240°C at 3°C/min. increment. Seven components were well separated, and each fraction was analysed by MS spectrum. Normal human urine was analysed by this method, the result of which is summarized in Table 3.抄録全体を表示PDF形式でダウンロード (679K)
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