巻号一覧
前身誌
55 巻, 3 号
選択された号の論文の6件中1～6を表示しています
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荻原 俊男, 西 啓子, 波多 丈, 熊原 雄一, 飯沼 一茂, 荒川 行信, 高木 淳, 倉田 邦夫1979 年 55 巻 3 号 p. 161-170
発行日: 1979/03/20
公開日: 2012/09/24
ジャーナル フリーA simplified direct radioimmunoassay for urinary acid labile aldosterone was developed. One ml of urine was hydrolysed with 2ml of 0.2N HC1 at 30°C for 16hrs. One tenth ml of hydrolysed urine diluted 10 times with charcoal treated aldosterone-free calf serum was used for the radioimmunoassay. The radioimmunoassay was done with a specific antibody, 125I-aldosterone, as the labeled antigen and polyethylene glycol for bound-free separation. There were excellent correlations between the present methods and other methods, i.e., i) a method using dichloromethane extraction before the assay as well as pre-extraction before hydrolysis and ii) a commercial kit using 3H-aldosterone. The intra-assay coefficient of variation was 5.8%, and the inter-assay coefficient of variation was 9.5%. The normal value of urinary aldosterone excretion was 3.7 ± 2.5pg/day by the present method, and values of patients with primary aldosteronism were between 24 to 43μg/day.抄録全体を表示PDF形式でダウンロード (646K) -
西村 典三, 末広 史恵, 三谷 裕昭, 井坂 勝利, 近森 一正, 森 博愛, 大島 一洋, 斎藤 史郎1979 年 55 巻 3 号 p. 171-182
発行日: 1979/03/20
公開日: 2012/09/24
ジャーナル フリーIn order to investigate changes in endocrine function, various tests were performed on eleven patients with anorexia nervosa. In two of them, endocrine function before and after treatment was also studied.
The responses of plasma LH and FSH to LH-RH were decreased in the patients as were plasma LH-RH, and LH and FSH responses to the oral administration of clomiphene citrate. In more than half of the cases, plasma TSH showed a delayed response to TRH. The basal levels of plasma GH were elevated, and plasma GH responses to hypoglycemia or 1-arginine were lowered in half of the patients. Plasma GH showed a paradoxical rise after glucose load in three out of six cases. Plasma IRI responses to 1-arginine load were decreased in some cases, but plasma IRG responses to 1-arginine were rather excessive. Plasma IRI after glucose load showed low or delayed responses in most of the cases. After the body weight was increased to normal levels by the treatment, pituitary and pancreatic endocrine functions were normalized.
These findings indicate that hypothalamic function is primarily impaired in this disease and causes nutritional disorder followed by the dysfunction of some endocrine organs. Such a possibility could be supported by the improvement of endocrine function after body weight has been recovered.抄録全体を表示PDF形式でダウンロード (972K) -
久保田 俊郎, 熊坂 高弘, 鈴木 明, 矢追 良正, 斉藤 幹1979 年 55 巻 3 号 p. 183-193
発行日: 1979/03/20
公開日: 2012/09/24
ジャーナル フリーSeveral reports revealed that catecholamine, which may play a significant part in supporting fetal growth and the function of the placenta or maintaining the pregnancy, exists in the feto-placental unit. In this experiment, we measured dopamine, one of the catecholamines, in decidual and villous tissues in early normal human pregnancies in order to elucidate the role of dopamine during pregnancy.
It is difficult to measure catecholamine concentrations in tissues with high sensitivity and specificity; therefore, we utilized two methods : the fluorimetric method and high pressure liquid chromatography.
The results were the following :
1) We extracted and measured dopamine from decidual and villous tissues by the fluorimetric method, and the dilution curve of the sample material was parallel with synthetic dopamine.
2) In the sixth or seventh week of pregnancy, dopamine concentrations were 110.57 ± 8.52 ng/lg wet weight (w.w.) in the decidua and 88.96 ± 20.38 ng/lg w.w. in the villi. In the former, it decreased gradually thereafter. In the latter, after it transiently increased to 130.78 ± 20.93 ng/lg w.w. in the eleventh week of pregnancy, it also decreased gradually. However, there was no significant difference between the value in the decidua and in the villi during the first trimester.
3) We extracted catecholamines from both tissues by means of high pressure liquid chromatography, and we measured dopamine and noradrenaline contents automatically by means of a fluorimetric spectromonitor. By this method, high. specific dopamine was proved to be in both tissues.
The results revealed that dopamine and noradrenaline were contained in decidual as well as in villous tissues of early pregnancy.抄録全体を表示PDF形式でダウンロード (985K) -
牧野 恒久, 横倉 恒雄, 飯塚 理八, 村木 篁1979 年 55 巻 3 号 p. 194-200
発行日: 1979/03/20
公開日: 2012/09/24
ジャーナル フリーInvestigations on various kinds of biological actions of a newly purified hypothalamic tridecapeptide, neurotensin, were performed both in vivo and in vitro by utilizing experimental animal models.
The effect of neurotensin on pituitary gonadotropin release was studied in ovariectomized estrogen-progesterone-treated rats by the measurement of serum levels of luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin (PRL) in radioimmunoassays. Neurotensin (340 ng/100 g BW) significantly increased serum LH and FSH 30 minutes after an intravenous injection (p<0.05) and lowered serum prolactin concentrations significantly (p<0.025). Bradykinin and substance P showed on significant effect on serum LH and FSH release.
Intraperitoneal or intravenous administration of 0.5 n.mole neurotensin lowered blood pressure in intact mature rats from the range of 90-100 mmHg to 50-60 mmHg; however, tachyphylaxis was observed after repeated injections of the same dose of this peptide.
Neurotensin was as potent as bradykinin in inducing rat duodenum relaxation and guinea pig ileum contraction in vitro. These effects of neurotensin and bradykinin on the intestines were not inhibited by the pre-treatment of phentolamine, propranolol, methysergide and pyribenzamine. Bradykinin induced contractions of the uterus in proestrous rats, but neurotensin induced no marked contraction.
These results suggest that neurotensin is not hypothalamic releasing or inhibiting factor but possess the nature of kinin.抄録全体を表示PDF形式でダウンロード (534K) -
臼倉 教臣, 吉光 康平, 相良 宝作, 岸谷 正雄, 坂戸 俊一, 川東 正範, 中林 肇, 竹田 亮祐1979 年 55 巻 3 号 p. 201-209
発行日: 1979/03/20
公開日: 2012/09/24
ジャーナル フリーWe made a study to determine if calcium and glucagon have an effect on insulin secretion, which is inhibited by somatostatin in normal dogs.
After an over-night fast, six mongrel dogs weighing 15 to 32 kg were anesthetized with an intravenous administration of sodium pentobarbital.
Calcium (0.8 mg/kg.b.w. of CaCl2) and glucagon (10-1 mg) were injected into the superior pancreaticoduodenal artery under the infusion of saline (1 ml/min) or cyclic somatostatin (50 μg bolus plus 5 μg/ml/min) through the pancreatic artery in 6 normal anesthetized dogs. Levels of plasma immunoreactive insulin (IRI) and glucagon (IRG) were estimated in the pancreatic vein and femoral artery by using the double antibody method and canine insulin standard and by employing anti-glucagon antibody AJ 15 (which crossreacted with glucagon-like immunoreactivity) and the method of dextran-coated charcoal. The saline and somatostatin infusions were conducted on the same dog at 20 minute intervals. Blood samples were collected from the pancreatic vein every 5 seconds for 2 minutes and thereafter every 1 min until 5 minutes had elapsed and from the femoral artery every 1 min.
The results were the following : 1. The calcium levels of the pancreatic vein increased from 4 to more than 8 mEq/L within one minute, whereas those of the femoral artery did not change significantly. 2. Under the saline infusion, CaCl2 evoked a sharp rise in the pancreatic venous IRI levels which started 25 seconds after the injection, rose to a peak of thirteen folds from baseline at 40 seconds and lasted for almost 2 minutes, whereas under the somatostatin infusion, the peak level was reached at 55 seconds and affected only one third of those under the saline infusion. 3. IRG levels in the pancreatic vein under the saline infusion did not show any significant changes after the CaCl2 injection and were higher than those under the somatostatin infusion at all points. 4. Under the somatostatin infusion, 10-1 mg of glucagon did not induce any rise at all in IRI concentrations of the pancreatic vein. But when CaCl2 was given just before the glucagon administration, a slight but significant rise in IRI was observed.
It is concluded that, in vivo, calcium has an insulin secretory effect under a normoglycemic state, and that somatostatin has an inhibitory action on both calcium- and cyclic-AMP-mediated insulin release.抄録全体を表示PDF形式でダウンロード (728K) -
坂本 忍, 今村 好久, 左雨 秀治, 岡本 良平1979 年 55 巻 3 号 p. 210-218
発行日: 1979/03/20
公開日: 2012/09/24
ジャーナル フリーIn order to keep a long-term lactation in primiparous SD strain rats with DMBA-induced mammary tumors, 5 pups aged 5 to 10 days were exchanged every 10 days. FSH, LH and prolactin (PRL) in pituitary tissues and FSH, LH, PRL, estradiol (E2) and progesterone (PRG) in plasma were determined during pregnancy (1, 2 and 3-week), at parturition (0 to 12 hours after parturition) and during lactation (1, 2, 3, 4, 5 and 6-week). Experimental rats were divided into 6 groups according to the lactating period (non-lactating, 3, 6, 8, 12 and 17-week). Blood samples were collected by cardiac puncture 1 hour after an intraperitoneal injection of urethane (150 mg/100 g body weight), and the pituitary gland was removed simultaneously.
During pregnancy, plasma PRL was low in the first and second week. PRL, E2 and PRG were high in the third week, during which DMBA-induced mammary tumors began to proliferate. PRG in plasma in the third week was lower than that of the second week. After parturition, the tumors regressed and did not develop for three weeks of lactation, during which plasma PRL was high whereas E2 and PRG were low. The tumors began to proliferate again from the fifth week of lactation, during which E2 was high and the estrous cycle reappeared.
The behavior of DMBA-induced mammary tumors in rats during pregnancy and lactation seems to be closely related to plasma estrogen levels.抄録全体を表示PDF形式でダウンロード (820K)
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