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60 巻, 7 号
選択された号の論文の6件中1～6を表示しています
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後藤 光弘, 水野 兼志, 松井 遵一郎, 国井 伸泰, 福地 総逸1984 年 60 巻 7 号 p. 835-845
発行日: 1984/07/20
公開日: 2012/09/24
ジャーナル フリーIn order to ascertain whether angiotensin I converting enzyme (ACE) activity might be regulated by thyroid hormone, serum ACE activity was measured in a variety of thyroid states, including hyperthyroid and hypothyroid subjects. In addition, the correlation of serum ACE activity to plasma renin activity (PRA) and plasma aldosterone concentration (PAC) was evaluated in these patients.
In hyperthyroid patients, the mean (± SD) serum ACE activity was 32.7 ± 6.7 U/ml (n= 30), which was significantly higher than that in hypothyroid patients (20.4 ± 4.3 U/ml, n=7, p<0.001) and in normal subjects (22.5 ± 3.4 U/ml, n=51, p<0.001). No significant difference in serum ACE activity was found between the hypothyroid patients and normal subjects. There was a significant positive correlation between serum ACE activity and PRA (r=0.524, n=30, p<0.01) and also between serum ACE activity and PAC (r=0.473, n=30, p<0.01) in the patients with hyperthyroidism. By contrast, no significant relationship was observed between serum ACE activity and thyroid hormones (r=0.115, for T3, r=0.143, for T4) in hyperthyroid patients. Treatment with furosemide (1 mg/kg i.v.) and upright posture (2 h) significantly increased PRA, PAC and serum ACE activity in both hyperthyroid patients and normal subjects, but not in hypothyroid patients. There was a significant positive correlation between changes in serum ACE activity and in PRA (r=0.418, n=23, p<0.05) in response to the treatment in hyperthyroid patients, while no significant relation-ship was observed between them in either hypothyroid patients (r=0.216, n=6, p<0.10) or normal subjects (r=0.620, n=10, 0.05<p<0.01). In one patient with hyperthyroidism, administration of propranolol decreased PRA from 3.4 to 2.3 ng/ml/h, corresponding to an apparent decrease in serum ACE activity from 38.7 to 29.6 U/ml.
From these results, it is suggested that serum ACE activity in the hyperthyroid state is modulated by the renin-angiotensin system rather than by thyroid hormone.抄録全体を表示PDF形式でダウンロード (939K) -
細田 一美, 市川 茂孝1984 年 60 巻 7 号 p. 846-851
発行日: 1984/07/20
公開日: 2012/09/24
ジャーナル フリーPregnant rats were ovariectomized on day 14 of pregnancy (sperm = day 1) and daily injected with 4 mg progesterone. The intrauterine pressure and volume of the conceptus with placenta of these rats and intact normal pregnant rats were measured every morning between day 14 and 20 of pregnancy.
The average volume of the conceptus in both groups increased at an almost constant rate until day 18 of pregnancy and rapidly between day 19 and 20 of pregnancy. The intrauterine pressure of ovariectomized progesterone-treated rats increased in parallel with the volume of the conceptus until day 19 of pregnancy, suggesting a deficiency in plasticity of the uterine wall. The pressure, however, decreased significantly between day 19 and 20 of pregnancy in spite of the rapid increase of the conceptus volume. In the control rats, the intrauterine pressure gradually decreased during the second half of pregnancy, whereas the conceptus volume increased.
The results suggest that estrogen secreted from the ovaries during the second half of pregnancy prevents the rise of the intrauterine pressure by improving the plasticity of the uterus. Furthermore, it could be considered that the decrease of the intrauterine pressure between day 19 and 20 of pregnancy in ovariectomized progesterone-treated rats may be caused by the estrogenic action of androgens secreted from the placenta in late pregnancy.抄録全体を表示PDF形式でダウンロード (675K) -
小島 元子, 斉藤 万一郎, 福地 総逸1984 年 60 巻 7 号 p. 852-871
発行日: 1984/07/20
公開日: 2012/09/24
ジャーナル フリーIn the present study, the effects of o, p'-DDD on plasma levels of pregnenolone, 17α-hydroxypregnenolone, progesterone, 17α-hydroxyprogesterone, 11-deoxycorticosterone, deoxycortisol, corticosterone, cortisol, androstenedione and testosterone were studied in 6 patients with adrenal carcinoma (3 with Cushing's syndrome, 2 with adrenogenital syndrome, one without clinical manifestation) and 6 with Cushing's diesease.
Plasma levels of these steroids were decreased in all of the patients with adrenal carcinoma. The decrement of progesterone and 17α-hydroxyprogesterone was greater than that of pregnenolone and 17α-hydroxypregnenolone. These results indicate that o, p'-DDD inhibits both cholesterol cleavage enzyme and 3β-hydroxysteroid dehydrogenase coupled with delta 5 to 4 isomerase system.
Plasma levels of pregnenolone and 17α-hydroxypregnenolone showed a twofold increase on the 7th day after consecutive administrations of o, p'-DDD in patients with Cushing's disease. Plasma levels of cortisol were decreased to normal one month after continuous o, p'-DDD treatment. Urinary 17-OHCS and 17-KS have been decreased out of proportion to the decrease in plasma cortisol in the first week of o, p'-DDD treatment. Such a disparity suggests that o, p'-DDD might affect the extra-adrenal metabolism of cortisol. However, no evidence was found for the inhibition of hepatic C17-20lyase and glucuronyl transferase.
Regression of pulmonary metastases was observed in one case with Cushing's syndrome due to adrenal carcinoma, suggesting that o, p'-DDD causes necrosis of the metastatic adrenal carcinoma. A remission of the disease was obtained in one patient with Cushing's disease after 6 months of continuous o, p'-DDD treatment. The usefulness of o, p'-DDD for the treatment of adrenal carcinoma with metastases and Cushing's disease was confirmed.抄録全体を表示PDF形式でダウンロード (1538K) -
森本 勲夫, 和泉 元衛, 長瀧 重信, 玉井 一1984 年 60 巻 7 号 p. 872-880
発行日: 1984/07/20
公開日: 2012/09/24
ジャーナル フリーNon-protein bound testosterone is considered to represent an active androgen in blood, and it reflects in androgenesity. We measured the dializable free testosterone (AFT) in various disorders. The values of plasma testosterone (T), fractional free testosterone (%FT) and AFT were 560 ± 151 (mean ± SD) ng/dl 1.9 ± 0.4% and 10.7 ± 3.3 ng/dl in normal males (n=43, aged 25-58), 51 ± 12 ng/dl, 1.2 ± 0.3% and 0.6 ± 0.2 ng/dl in normal females (n=30, aged 21-41), and 63 ± 26 ng/dl, 0.6 ± 0.3% and 0.4 ± 0.2 ng/dl in pregnancy (n=20, second and third trimester), respectively. Those values in normal females and pregnancy were significantly lower than those in normal males (p<0.001).
In male, the concentrations of T and AFT in patients with primary (n=43, aged 18-50) and secondary hypogonadism (n=16, aged 22-66) were significantly decreased (p<0.001 vs normal males), respectively. The plasma concentration of total testosterone (T) was increased 1032 ± 269 ng/dl in chronic hepatitis (p<0.001) (n=21, aged 28-52) and 1455 ± 366 ng/dl in hyperthyroidism (p<0.001) (n=44, aged, 18-52). However, %FT in those disorders was significantly decreased 1.2 ± 0.3% in chronic hepatitis (p<0.001) and 0.8 ± 0.3% in hyperthyroidism (p<0.001), and plasma levels of AFT in both disorders were not different as compared with that in normal males. The closed negative correlationship between the values of T and %FT in normal men, male patients with chronic hepatitis and hyperthyroidism was observed (r=-0.78, p<0.001).
In female, the levels of T and AFT in patients with primary (n=7, aged 21-38) and secondary hypogonadism (n=16, aged, 22-66) were significantly lower (p<0.01) than those in normal females. However, %FT in those disorders were not different as compared with that in normal females. Hyperthyroid patients had higher levels of T (p<0.05), lower %T (p<0.01) and lower T (p<0.05) than those of normal females, respectively. However, hypothyroid patients had normal levels of T and AFT. The levels of T, %FT and AFT in patients with hirsutism (n=8, aged 20-39) were 81 ± 36 ng/dl, 1.9 ± 0.6% and 1.5 ± 0.7 ng/ dl, respectively, and those values were significantly higher (p<0.01) than those in normal females. The plasma concentrations of testosterone in three patients with Cushing's syndrome (n=3, aged 26-34) were within the normal range, but, %FT and AFT were higher than those in normal females.
We concluded the measurement of free testosterone can provide more information for the understanding of androgenesity than total testosterone.抄録全体を表示PDF形式でダウンロード (857K) -
飯高 誠1984 年 60 巻 7 号 p. 881-891
発行日: 1984/07/20
公開日: 2012/09/24
ジャーナル フリーAntibodies against thyroid cell surface antigens have been demonstrated by indirect immunofluorescence on viable cells and by mixed hemadsorption using monolayer cell culture. Recently quantitative assays using thyroid plasma membrane or cultured thyroid cell were also reported. The present study reports a novel quantitative assay for the detection of anti-thyroid plasma membrane antibody (APA) using solubilized and immobilized thyroid plasma membrane.
Thyroid plasma membrane purified by sucrose density gradient centrifugation was solubilized with Triton X-100 and coupled to CNBr activated Sepharose 4B. Sera from patients with various thyroid diseases were incubated with this solid phase. Five microliters of serum was sufficient for the assay. After extensive washing, immunoglobulin G (Ig G) or immunoglobulin M (Ig M) bound to thyroid plasma membrane was detected by horseradish-peroxidase labeled rabbit anti-human Ig G or Ig M antibody.
Significantly elevated values of Ig G class APA (Ig G-APA) were detected in the sera from patients with Graves' or Hashimoto's disease. Some of the patients with thyroid adenoma also showed positive Ig G-APA, although all of the patients with thyroid cancer had negative Ig G-APA. A significant correlation between Ig G-APA and anti-thyroid microsomal antibody (AMA) among patients with autoimmune thyroid diseases was observed. No significant correlation, however, was found between anti-thyroglobulin antibody (ATA) and Ig G-APA. A large amount of thyroglobulin (Tg), which was reported to have the receptor in thyroid plasma membrane, inhibited the binding of Ig G-APA does dependently. Ten micrograms of ATA and one milligram of bovine serum albumine, however, had no influence on the binding. Ig G-APA also significantly correlated with thyrotropin binding inhibitor immunoglobulin (TBII). The binding, however, was not inhibited by one hundred milliunits of TSH. These findings suggested that most of Ig G-APA was bound to different sites from TSH receptor, although some part of Ig G-APA might have TBII activity.
Ig M class APA (Ig M-APA) among various thyroid diseases was also detected. In Graves' disease, more than sixty percent of patients had positive Ig M-APA. There was no significant difference in the value of Ig M-APA between untreated and treated patients. Positive Ig M-APA was found even in some euthyroid patients. Ig M-APA in Graves' disease correlated with neither AMA nor TBII. Some of patients with Hashimoto's disease and subacute thyroiditis also showed positive Ig M-APA.
APA, which might have some important influence on the thyroid cell, was detected in the great majority of patients with autoimmune thyroid diseases by solid phase enzymeimmunoassay. The method is simple and sensitive. Studies about APA could contribute further to the understanding of the immunological role in the pathogenesis of autoimmune thyroid diseases.抄録全体を表示PDF形式でダウンロード (981K) -
三浦 良, 弓田 滋, 孫 孝義, 古川 洋太郎, 桜田 俊郎, 斎藤 慎太郎1984 年 60 巻 7 号 p. 892-898
発行日: 1984/07/20
公開日: 2012/09/24
ジャーナル フリーThe parameters of calcium metabolism were determined in 22 patients with untreated hyperthyroidism (5 males and 17 females) and 5 control subjects.
Hypercalcemia was found in the patients with hyperthyroidism in comparison with the control subjects (serum Ca : 10.0 ± 0.56 vs. 9.0 ± 0.18, p<0.001 and Ca++ : 5.1 ± 0.28 vs. 4.6 ± 0.15 mg/dl, p<0.001, mean ± SD). Although the urinary excretion of calcium was decreased in many patients, abnormalities of phosphate metabolism were not found in this study. The parameters of bone resorption, urinary hydroxyproline, serum alkaline phosphatase and acid phosphatase, were increased in all patients with hyperthyroidism. Serum immunoreactive PTH was decreased (0.23 ± 0.05 vs. 0.29 ± 0.05 ngEq/ml, p<0.05). In vitamin D metabolites, 25-OH-D did not differ from the control (16.9 ± 7.76 vs. 17.9 ± 5.52 ng/ml), 1, 25-(OH) 2D showed a tendency to decrease (32.6 ± 19.53 vs. 37.2 ± 13.75 pg/ml) and 24, 25-(OH) 2D was obviously increased (5.57 ± 3.582 vs. 1.73 ± 0.619 ng/ml, p<0.001) in the hyperthyroid patients.
Thus, the parathyroid function was suppressed in the patients with hyperthyroidism, and hypercalcemia in hyperthyroidism was suggested to be due to the direct action of thyroid hormone upon the bone.抄録全体を表示PDF形式でダウンロード (577K)
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