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61 巻, 9 号
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鳥居 宣夫1985 年 61 巻 9 号 p. 859-871
発行日: 1985/09/20
公開日: 2012/09/24
ジャーナル フリーCa ions and cAMP are known to be involved in the action of hormones.
In this study, the actions of Ca ions and cAMP in ACTH lipolysis were investigated by comparing ACTH1-24, ACTH1-10 and ACTH11-24 with regard to : lipolytic activity, binding affinity to the fat cell membrane, changes in Ca ion binding to the fat cell membrane and cAMP formation. Rat adipocytes were used.
Lipolytic activity was measured in terms of the amount of FFA released from fat cells into the medium after incubation with the ACTH analogs at 37°C for 30 minutes.
Binding affinity to the fat cell membrane was expressed as competitive binding affinity, determined by simultaneously incubating fat cell ghosts with 125I-ACTH1-24 and unlabelled ACTH analogs at 4°C for 40 minutes.
Changes in Ca ion binding to the fat cell membrane was expressed as Ca binding capacity, measured by simultaneously incubating fat cell ghosts with 45 Ca and ACTH analogs at 24°C for 20 minutes.
CAMP formation was measured by RIA as the amount of cAMP formed in fat cells incubated with 10-6M ACTH analogs at 37°C for 0, 3, 5 and 10 minutes.
FFA release after incubation with each of the ACTH analogs at 10-6M was 1.17mEq/ L/tube for ACTH1-24, 0.26mEq/L/tube for ACTH1-10 and 0.25mEq/L/tube for ACTH11-24 showing a high value only for ACTH1-24.
The binding affinity to the fat cell membrane (high affinity constant) was 4.4×10-8 for ACTH1-24, 4.6×10-8M for ACTH1-10 and 4.6×10-8M for ACTH1-24. There were no significant differences among the binding affinities of these ACTH analogs.
The binding capacity of 45Ca++ to the fat cell membrane (high affinity constant) was 3.64×10-6M for ACTH1-24, 4.49×10-6M for ACTH1-10 and 5.46×10-6M for ACTH11-24.
There were no significant differences among these ACTH analogs.
The increase in cAMP formation during the first 3 minutes of incubation was 35.5 fmol/mg dry weight of fat cells for ACTH1-24, 2.2fmol/mg for ACTH1-10 and 1.8fmol/mg for ACTH11-24. An increase was only observed for ACTH1-24.
The fact that FFA release and cAMP formation were high only with ACTH1-24 among the ACTH analogs tested indicates that cAMP formation plays an important role in the lipolytic activity of ACTH in fat cells and that the three-dimensional structure of ACTH is important for cAMP formation.抄録全体を表示PDF形式でダウンロード (1088K) -
丸 義朗, 立川 幸治, 平岩 望, 荻野 良郎, 紫芝 良昌, 渡辺 五朗, 中澤 英樹, 鶴丸 昌彦, 秋山 洋, 松下 央1985 年 61 巻 9 号 p. 872-881
発行日: 1985/09/20
公開日: 2012/09/24
ジャーナル フリーA family of multiple endocrine neoplasia type I with five confirmed cases in three generations is described. All of them have primary hyperparathyroidism in common. The propositus is 51 year-old male. After a year of symptoms of gastroduodenal ulcer, he was found to have elevated levels of serum gastrin and PTH. The serial imaging studies revealed a tumor in pancreatic head, and Zollinger-Ellison syndrome was diagnosed. The gastrin level was reduced into normal range after extirpation of the tumor, but post surgical elevation of Calcium put the patient under parathyroidectomy, which normalized serum PTH and Calcium levels. His two sisters (I and II), the mother of them, and the daughter of sister I, had neither signs nor symptoms until family study showed hypercalcemia in all. Sister I is a 54 year-old female with enlarged parathyroid. The hyperparathyroidism is of chemical type, but no other endocrinological abnormality is found. The Calcium level decreased after parathyroidectomy. Sister II is a 56 year-old female. The only sign was galactorrhea. Serum PTH and Calcium decreased after parathyroidectomy. The prolactinoma was diagnosed by the increased prolactin levels and enhanced mass lesion in sella turcica. Her serum prolactin levels is now within normal range since she is on bromocryptine. The mother of the above three siblings and the daughter of the sister I are now under further study.抄録全体を表示PDF形式でダウンロード (2397K) -
in vitro絨毛培養系での検討松尾 博哉, 丸尾 猛, 保科 真, 望月 眞人1985 年 61 巻 9 号 p. 882-892
発行日: 1985/09/20
公開日: 2012/09/24
ジャーナル フリーIn order to elucidate the inhibitory regulation of hCG synthesis, the dynamics of the synthesis and secretion of hCG was investigated by culturing explants of trophoblastic tissues from early placenta and choriocarcinoma in the presence and absence of progesterone, and these results were then compared to those of hPL. The dynamics of mRNAs encoding hCG (α, β) and hPL was assessed by grain counts in the tissue sections hybridized in situ with labelled cDNA probes corresponding to these mRNAs. In the control culture of early placenta, hCG, hCGαa and hPL in the medium showed marked increases compared to the initial tissue levels, whereas hCG, hCGα and immunoreactive hCGβ in the tissue explants remained constant. Progesterone suppressed the secretion of hCG and hCGα by placental tissue after 48-hour culture in a dose-response manner and simultaneously decreased the tissue level of immunoreactive hCGβ. However, neither hPL secretion nor hPL tissue level were decreased by progesterone. The in situ hybridization revealed that grain counts for hCGα-mRNA and hCGβ-mRNA in the sections prepared from placenta cultured with progesterone were decreased compared to those prepared without progesterone, where-as grain counts for hPL-mRNA remained unchanged. In culture of explants from chorio-carcinoma, no inhibition of synthesis and secretion of hCG by progesterone was found. From these results it will be concluded that progesterone inhibits hCG synthesis through the inhibition of hCGα-mRNA and hCGβ-mRNA accumulation and that regulation of hCG synthesis in choriocarcinoma is different from that in the normal placenta.抄録全体を表示PDF形式でダウンロード (1158K) -
丸山 裕, 新津 洋司郎, 高橋 康二, 平田 康二, 本間 久登, 漆崎 一朗1985 年 61 巻 9 号 p. 893-911
発行日: 1985/09/20
公開日: 2012/09/24
ジャーナル フリーA new isozyme of Glutathione-S-transferase (GST) with more acidic pI (6.7) than other forms of GST hitherto reported was isolated from rat liver cytosol by consecutive chromatographies on a DEAE cellulose column, lysyl-GSH affinity column and Sephadex G-100 column.
This anionic form of GST represented approximately one third of total GST activity in rat liver cytosol.
Amino acid composition, immunological reactivity, enzymatic properties, and secondary structure as measured by circular dichroism of this form were distinct from those of cationic isozymes (GST-AA, GST-B, GST-X), presently investigated.
The stoichiometric ratio of high affinity site for bilirubin to GST molecule differs amongst isozymes.
The anionic form of GST bound two bilirubin per molecule whereas cationic GSTs bound only one bilirubin per two subunits.
The most distinguished property of anionic GST was its strong affinity for glucocorticoid. The dissociation constant of anionic GST-corticosterone complex was as low as 2.0×10-8M.
Corticosterone inhibited the enzyme activity of anionic GST in a noncompetitive fashion with an apparent Ki value of 8.6×10-5M and 1.1×10-6M for 1-chloro-2.4-dinitrobenzene and GST respectively.
The anionic GST-corticosterone complex bound to DNA coupled Sepharose at 25°C and passed through the column at 4°C. Conversely, the complex bound to a DEAE cellulose column at 4°C but passed through at 25°C. These properties of anionic GST are quite similar to those of glucocorticoid receptor of rat liver cytosol reported previously.抄録全体を表示PDF形式でダウンロード (2025K) -
孫 孝義, 古川 洋太郎, 弓田 滋, 三浦 良, 吉永 馨, 山根 良太1985 年 61 巻 9 号 p. 912-923
発行日: 1985/09/20
公開日: 2012/09/24
ジャーナル フリーSeveral problems in the measurement of plasma cyclic AMP (PcAMP) and nephrogenous cyclic AMP were studied using a YAMASA RIA Kit (YAMASA Shoyu, Choshi, Japan). In this assay method, cAMP in plasma is directly succinylated without prior deproteinization, and then it is bound to antibody in an imidazole buffer.
1. So far as the blood samples were obtained with EDTA-4Na at least 5.0mM in the final concentration, PcAMP was not reduced until 16 hours after the blood samples were drawn. Even without EDTA, the reductions in PcAMP were not detected within 1 hour after the blood samples were drawn.
2. This assay method for PcAMP showed parallelism in the dilution curve. Recovery was almost complete. Intra-and interassay variations were low. When plasma was incubated at 37°C for 24 hours, PcAMP became negligible. Furthermore, the values of PcAMP measured with this direct assay system almost agreed with those obtained after the purification by deproteinization and Dowex column chromatography through an anion-exchange resin. The normal values of PcAMP were 13.6 ± 3.62 pmol/ml [mean ± SD, n = 43].
3. Nephrogenous cAMP expressed as a function of GFR never did show any negative values in various clinical situations. From the data of basal levels and the oral calcium tolerance test, nephrogenous cAMP appeared to be more useful than total urinary cAMP in the diagnosis of parathyroid disorders, especially hyperparathyroidism.
In conclusion, the sampling of blood with EDTA is sufficient to inhibit the spontaneous decrease of cAMP in plasma. The direct assay method for PcAMP with a YAMASA RIA Kit provides qualitative and quantitative specificity, and the values determined by this assay method are comparable to those following the purification procedures. Nephrogenous cAMP, being readily measurable with this Kit, is extremely useful as an index of parathyroid function.抄録全体を表示PDF形式でダウンロード (1084K) -
インスリンレセプター異常による著明なインスリン抵抗性を示した女児例郷司 克己, 高田 康光, 小林 正1985 年 61 巻 9 号 p. 924-935
発行日: 1985/09/20
公開日: 2012/09/24
ジャーナル フリーThis report describes a 3-month-old female infant with the typical physical features of leprechaunism. The patient demonstrated glucose intolerance and marked hyperinsulinemia (4600μU/ml). Since an intravenous insulin injection (actrapid insulin : 0.15U/kg) caused no significant decrease in the blood glucose level, the presence of insulin resistance was suggested. Neither insulin antibodies nor insulin receptor antibodies were found in the patient's plasma, and other circulating insulin antagonists such as glucagon, growth hormone, 1. and cortisol were within normal limits. [125 I] Insulin binding to the erythrocytes from the patient was as low as 1.02% (control infants : 4.89± 1.08% [mean±SD]). [125I] Insulin binding to the cultured transformed lymphocytes from the patient was similarly reduced to 3.58% (control : 20.9±2.71% [mean±SD]). From these findings we concluded that the insulin resistance was due to a primary defect in insulin receptors.
Interestingly, transient remissions of the patient's glucose intolerance and hyperinsulinemia were observed during a year of follow-up study. The insulin tolerance test which was performed at the remission period showed an improvement in insulin resistance. However, the insulin binding defect to erythrocytes remained unchanged even at the remission period. The exact cause of these remissions was not clear and remained to be elucidated.抄録全体を表示PDF形式でダウンロード (1796K)
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