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66 巻, 10 号
選択された号の論文の5件中1～5を表示しています
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原 秀雄, 伴 良雄, 谷山 松雄, 佐藤 龍次, 九島 健二, 長倉 穂積, 海原 正宏, 伊藤 國彦1990 年 66 巻 10 号 p. 1075-1084
発行日: 1990/10/20
公開日: 2012/09/24
ジャーナル フリーSerum fructosamine was measured in patients with Graves' disease and primary hypothyroidism. Fructosamine levels and fructosamine per albumin ratio were significantly lower in patients with Graves' disease than in normal subjects, while they were significantly higher in patients with primary hypothyroidism. Fructosamine levels were normalized by treatment in the euthyroid state in patients with Graves' disease. Along with the normalization of thyroid stimulating hormone levels, fructosamine levels returned to the normal range in patients with primary hypothyroidism with treatment. There were significant correlations between fructosamine levels and free triiodothyronine levels, free thyroxine levels, thyroid stimulating hormone levels, hemoglobin Alc levels, albumin levels, and creatine phosphokinase levels.
We concluded that it was useful to measure serum fructosamine as an indicator of peripheral metabolic function in patients with thyroid diseases.抄録全体を表示PDF形式でダウンロード (651K) -
高感度Immunofluorometric assay (IFMA) を用いた検討三熊 直人, 和田 英樹, 熊本 悦明1990 年 66 巻 10 号 p. 1085-1093
発行日: 1990/10/20
公開日: 2012/09/24
ジャーナル フリーPDF形式でダウンロード (867K) -
阪上 享子1990 年 66 巻 10 号 p. 1094-1107
発行日: 1990/10/20
公開日: 2012/09/24
ジャーナル フリーWith a highly sensitive time-resolved fluorometric immunoassay (TR-FIA), serum thyrotropin (TSH) levels were determined in various conditions in healthy subjects. In addition, we compared the thyroid function in 10 depressed female patients with that in 27 female controls.
1) We evaluated a highly sensitive time-resolved fluorometric immunoassay kit for serum TSH. The lower limit of detection of TSH in serum was 0.008<U/ml. The intraassay and interassay variances were 3.0>3.6% and 3.4>5.1%, respectively. There was a significant correlation between basal TSH levels and maximum TSH values after TRH administration (r=0.797, p<0.01).
2) The mean TSH levels in 31 healthy controls of both sexes was 1.26±0.96<U/ml, but TSH levels in women were significantly higher than in men (p<0.01). A large intra-individual variation of serum TSH levels determined on different days was found equally in both men and women. The nyctohemeral elevation of TSH levels was not clearly seen prior to the onset of normal sleep, but the nocturnal rise of TSH levels was remarkably accentuated by sleep deprivation.
3) The serum TSH levels in depressed female patients were significantly lower than those in healthy female controls when the post-menopausal subjects were excluded. For the serum thyroid hormone concentrations, serum T4 levels were normal. Serum free T3 levels tended to be lower, although the reduction was not significant. The serum levels of these 3 thyroid hormones were not related to serum TSH values.
The present study demonstrated a large variation of TSH levels in various conditions, even in the same individuals, indicating the necessity of strictly controlled conditions in the study on TSH secretion. A significant reduction in TSH levels was observed in the depressed female patients when the post-menopausal subjects were excluded. Our results suggest that the dysfunction of the regulating mechanism of the pituitary-thyroid axis in depression may occur at a pituitary or a suprapituitary level.抄録全体を表示PDF形式でダウンロード (1350K) -
cross-linking法を用いた受容体構造の検討長尾 宗彦, 坂本 長逸, 的崎 尚, 西崎 朗, 誉田 芳孝, 中野 修, 馬場 茂明1990 年 66 巻 10 号 p. 1108-1116
発行日: 1990/10/20
公開日: 2012/09/24
ジャーナル フリーWe characterized structually the receptors for somatostatin in rat cerebral cortex by affinity labeling with [125I-Tyr1] somatostatin. [125I-Tyr1] somatostatin was cross-linked to cerebrocortical membranes using photoreactive cross-linker: N-5-azido-2-nitrobenzoyloxy-succinimide. Analysis by autoradiography revealed a broad band centered at Mr=72,000 in the presence or absence of dithiothreitol. Affinity labeling of and specific [125I-Tyr1] somatostatin binding to cerebrocortical membranes were decreased similarly by adding unlabeled somatostatin or nonhydrolyzable guanine nucleotide analogue, guanyl-5′-yl imidodiphosphate, in a dose dependent manner. The pretreatment of cerebrocortical membranes with islet activating protein resulted in a decrease in subsequent affinity labeling of the protein. The cross-linked protein could be solubilized with Zwittergent 3-12 and poorly with digitonin, triton X-100 and NP-40. When exposed to agarose-coupled lectins, the solubilized labeled protein was absorbed to wheat germ agglutinin, partially to ricin communis-II, and not to concanavalin A or lentil lectin. The Mr=72,000 protein bound to wheat germ agglutinin-agarose was eluted with not only N, N′, N″-triacetylchitotriose but also N-acetylglucosamine. These results suggest that somatostatin receptors on cerebrocortical membranes are a monomeric glycoprotein with a Mr=70,000 containing no disulfide-linked binding subunit, which is coupled to islet activating protein-sensitive guanine nucleotide regulatory protein.抄録全体を表示PDF形式でダウンロード (2723K) -
宮下 尚夫, 清水 幸子, 橋野 正史, 千葉 博, 小崎 俊男, 斉藤 裕, 矢内原 巧, 大沢 由男1990 年 66 巻 10 号 p. 1117-1125
発行日: 1990/10/20
公開日: 2012/09/24
ジャーナル フリーThe conversion of androgen to estrogen is mediated by aromatase activity, and 19-hydroxylase and C10-19lyase are seen to be involved in C19-demethylation. The present study is to demonstrate C10-19lyase activity in adrenal tissue. Fetal bovine and pig adrenal tissue homogenates were incubated with14 C-androstenedione (A) and NADPH under air. The products 14C-19-OHA, 14C-19-norA, with added carrier standard 3H-19-OHA, 3H-19-norA were separated and purified by T. L. C.. The identification of 19-norA was established by recrystallization and that of 19-OHA by C. C. D.. The 3H/14C ratios of the products 19-norA were found to be constant in repeated crystallization. The amount of 19-OHA from A in fetal adrenal tissue was 30.0nmol/h/g protein, and that of 19-norA from A was 12.59pmol/h/g protein. C10-19lyase activity from 19-OHA, calculated by the amounts of 19-norA in fetal, bovine and pig adrenal tissue, was 49.7, 3.50, 10.3pmol/h/g protein, respectively. This is the first report to demonstrate androgen C10-19lyase in human fetal adrenal tissue, and it is assumed that fetal adrenal tissue forms 19-norA via 19-OHA.抄録全体を表示PDF形式でダウンロード (834K)
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