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Volume 19, Issue 12
Displaying 1-10 of 10 articles from this issue
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Hiroo IIDA1964 Volume 19 Issue 12 Pages 463-468
Published: December 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESS1. With proteolytic group ofClostridium botulinum, marked decrease of turbidity of culture fluids is observed, suggesting the lysis of bacteria in the early stage of cultivation. On the other hand, the decrease of turbidity is not so conspicuous with non-proteolytic group.
2. The pH level of culture fluids attained by proteolytic group is always higher than that attained by non-proteolytic group.
3. The phenomenon of toxin activation by trypsin is obviously demonstrated with proteolytic type B, strain Lamanna, as well as with non-proteolytic type B, strain QC and type E, strains VH and Iwanai. On the other hand, the activation seems to be rather uncertain with non-proteolytic type C, strains Stockholm and 468 and type D, strain 1873, as well as proteolytic type A, strain 190.View full abstractDownload PDF (656K) -
Michio TSUKAMURA, Haruo TOYAMA, Shoji MIZUNO1964 Volume 19 Issue 12 Pages 469-471
Published: December 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSMycobacterium “Jucho” and “Takeo” are strains widely used in Japan for various experiments. They were isolated asM. aviunz, but recently questions have been presented on their classification. The present study has been conducted to make clear taxonomic position of these strains.
So far as tested on the strains maintained in our laboratory, these two strains showed characters quite similar to the description ofM. smegntatisin Bergey's Manual of Determinative Bacteriology (7 th ed., 1957). The results of the Bonicke's amidase-test also showed a pattern characteristic forM. smegmatis.
In view ofthe results obtained, these strains have been thought to beM. smeginatis.View full abstractDownload PDF (400K) -
1. Effect to Endotoxins on Salmonella Typhimurium Intracerebral Infection of MiceYukihiro OHTA1964 Volume 19 Issue 12 Pages 472-479
Published: December 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSThere have been many recent studies on the phenomenon of “nonspecific” aquired resistance to infection and intoxication. It has been shown repeatedly that injection of vaccines or endotoxins prepared from Gram-negative bacteria alters host susceptibility to subsequent infections with homologous or heterologous bacteria.
The present investigations were undertaken to compare the effects of the pretreatments with some bacterial endotoxins and their pretreatment routes on resistance of mice to typhoid cerebritis.
Results:
1. The effects of three different pretreatment routes, cerebral, peritoneal and subcutaneous, were compared on the protection of mice encephalitis. Cerebral treatment was shown to be the most effective one in these three routes.
2. Endotoxins were more effective than heat killed vaccines and the homologous endotoxin prepared from Salmonella typhimuriumwas more effective than the other two endotoxins prepared from heterologus bacteria.
3.Pretreatment with small doses of endotoxin could not protect mice from intracerebral challenge with lethal dose of endotoxin 48 hr after the pretreatment.View full abstractDownload PDF (1138K) -
Tadaiku UEYAMA, Tsuyoshi BABA, Yukio BITO1964 Volume 19 Issue 12 Pages 480-482
Published: December 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSToxic substance was isolated from the cell and culture filtrate of Vibrio parahaemolyticusthrough ammonium sulfate fractionation, hydrochloric acid precipitation, manganese chloride precipitation and zone electrophoresis.
These substance was positive in Biuret reaction and BPB staining and showed the absorbtion of maximum in 280 mμ, while negative in sugar and nucleic acid reaction
These facts strongly suggest this substance to be protein like.View full abstractDownload PDF (435K) -
Comparison of Peptones with Pathogenic Halophiles as Test OrganismYukio YAMAZI, Masami TAKAHASHI, Kazuo KURIYAMA, Tsuneo KOZIMA1964 Volume 19 Issue 12 Pages 483-488
Published: December 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSTwenty three samples of peptones were compared on the capacity and suitability for the growth and tests of some biochemical activities, and on slime layer production of 29 strains of Vibrio parahaemolyticus.
In general, peptones from meat, especially of C Co., gave good growth.
For the indole reaction, peptones from milk casein of C Co., Bacto-Trypton, and that from soya bean of D Co. were observed suitable ; and those from meat often gaveerroneous results. In the test for gelatin liquefaction, all peptones tested gave good result. Hydrogen sulfide was not produced by all test strains in Kligler medium from all peptones tested, i. e. all peptones were suitable. For reduction of methylene blue, 2 of 3 samples of peptones from soya bean were not suitable.
Large quantity of extracellular slime layer substance was produced on agar media from mixed peptones which were composed of those from milk casein and meat, of D Co. and of A Co., and on that from soya bean of D Co.View full abstractDownload PDF (922K) -
2. The “Auto-plaque” and “Dismune” Phenomena of a Saprophytic Mycobacterium, Jucho, Lysogenized by Y 13 L PhageYasuo MIZUGUCHI1964 Volume 19 Issue 12 Pages 489-493
Published: December 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSIt was found that “auto-plaques” were produced without addition of any indicatorstrain on the lawn of Myc. Jucho, which was preliminarily lysogenized by Y 13 L phage, subcultured on Ogawa's egg slant and seeded on agar plate, and that the bacterial growth was inhibited in the same time, on the similarly seeded agar plate with the same Mycobacterium, by spotting a phage suspension of Y 13 L. However, no plaquewas produced on agar plate or Ogawa's egg plate seeded with the same bacterial culture maintained on agar media.
Turbidity of the “auto-plaque” depended on the culture age of the lysogenic strainof Ogawa's egg slant from which bacterial suspension seeded was prepared, namely, the older became the culture, the clearer became the plaque.
Addition of Calcium chloride at a cencentration of 2x10-3 Moliml to Ogawa's madia, on which the bacteria was subcultured, inhibited the “auto-plaque” formation completely.
Analytical studies suggestedthat the “auto-plaque” and “dismune” phenomena described above were produced by neither a virulent mutant from Y 13 L phage nor a sensitive mutant population derived from lysogenic Jucho.
The number of colonies produced on Ogawa's egg plate by seeding a bacterial suspension of the lysogenic Jucho prepared from Ogawa's slant was about three times as much as that of the agar plate.
These investigations seem to suggest thepossibility of phage induction of lysogenic Jucho strain by changing the culture media from Ogawa to agar.View full abstractDownload PDF (789K) -
Sensitivity of Vibrio Species Against Pteridine DerivativesTsunesaburo FUJINO, Yoshifumi TAKEDA, Yoshiya AKITA, Toshihide TAMURA1964 Volume 19 Issue 12 Pages 494-498
Published: December 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSSensitivity of Vibrio species especially ofVibrio parahaemolyticusagainst various pteridine derivatives were examined. 2: 4 diamino 6: 7 di-n-propyl pteridine (O/69) has the vibriostatic activity as well as 2: 4 diamino 6: 7 di-iso-propyl pteridine (O/129), which is recognised as vibriostatic agent.
2: 4 diamino 6: 7 di-iso-propyl pteridine synthesized by Dr. Saikachi was equally active as vibriostatic agent (O/129) and the possibility of itssynthesis in our country was discussedView full abstractDownload PDF (2549K) -
I. Heterologous Immunogenicity of Phenol-killed Bacterial CellsKanzi TSUCHIYA, Toshiyuki YAMASAKI1964 Volume 19 Issue 12 Pages 499-506
Published: December 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSPhenol-killed cells of various strains of mycobacteria increased the resistance of mice against the lethal infection of virulent staphylococci. In this subsequent studies, the phenomenum of heterologous immunogenicity has been investigated in details.
Resistance of mice against staphylococcal infection was increased by the immunization with either intraperitoneal or subcutaneous injection of phenol-killed BCG cells. As far as protective effectis concerned, intraperitoneal injection afforded better protection than subcutaneous one. As to the material used for cell suspension, phenol-killed BCG cells suspended in Drakeol gave high immunogenicity than the cells suspended in distilled water. Heterologous immunogenic activity was obtained at the doses lower than 0.001 mg in the case of Drakeol suspension, whereas 0.1mg for intraperitoneous or 1mg for subcutaneous route is necessary to obtain comparative immunogenicity in the case of water suspension.
The resistance of mice against the infection persisted for two to three weeks, reaching its highest activity at the third to fifth day after the injection of killed cells.
This protective effect of BCGcells extended not only against infection of Gram-positive bacteria but also against attack of Gram-negative bacteria.View full abstractDownload PDF (1121K) -
1964 Volume 19 Issue 12 Pages 507-522
Published: December 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (3346K) -
1964 Volume 19 Issue 12 Pages 523-542
Published: December 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (4137K)
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