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Volume 37 (1982)
- Issue 6 Pages 895-
- Issue 5 Pages 811-
- Issue 4 Pages 739-
- Issue 3 Pages 583-
- Issue 2 Pages 457-
- Issue 1 Pages 42-
Volume 37, Issue 5
Displaying 1-6 of 6 articles from this issue
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Toshio MIWATANI1982 Volume 37 Issue 5 Pages 811-828
Published: September 25, 1982
Released on J-STAGE: February 19, 2009
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Tsutomu NISHIHARA1982 Volume 37 Issue 5 Pages 829-838
Published: September 25, 1982
Released on J-STAGE: February 19, 2009
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Host functions required for Tn5 transposition and Regulation mechanism for DNA replication of Inc FII R plasmidsChihiro SASAKAWA, Hirofumi DANBARA1982 Volume 37 Issue 5 Pages 839-849
Published: September 25, 1982
Released on J-STAGE: February 19, 2009
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Hisako HARA1982 Volume 37 Issue 5 Pages 851-861
Published: September 25, 1982
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSPseudomonas halosaccharolytica was grown under two extreme growth conditions of low temperature-low NaCl concentration (20C-0.5M NaCl) and high temperature-high NaCl concentration (40C-3.5M NaCl). High temperature-high NaCl-grown cells contained large amounts of saturated and cyclopropanoic fatty acids and acidic phospholipids, whereas low temperature-low NaCl-grown cells had decreased concentrations of these acidic phospholipids and fatty acids with increased concentrations of the corresponding unsaturated fatty acids. 14C tracer experiments with the resting cells showed that the high NaCl concentration inhibited the synthesis of phosphatidylethanolamine, but not that of phosphatidylglycerol. Liposomes of lipids extracted from the cells grown at 40C in 3.5M NaCl showed an increased the activation energy for altering the NaCl concentration of liposome buffer from 0.5M to 3.5M, whereas those of extractable lipids of the cells grown at 20C in 0.5M NaCl did not show such a change.View full abstractDownload PDF (1176K) -
Akiko TSUGAWA1982 Volume 37 Issue 5 Pages 863-873
Published: September 25, 1982
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe chromosome of a transducing phage λrifd18 carries a set of rRNA genes of E. coli. I have studied in vitro requirements for the preferential transcription of rRNA using λrifd18 DNA as a template. The following results were obtained: (1) Addition of Rs factor purified from ribosome wash stimulated total RNA synthesis. (2) With crude RNA polymerase prepared from extracts of E. coli, 17S and 23S premature rRNAs were synthesized, comprising 50% of the total transcription. Addition of guanosine tetraphosphate (ppGpp) resulted in selective inhibition of the rRNA synthesis. (3) When purified RNA polymerase was used, 7S, 12S and 26S transcripts were synthesized by addition of termination factor ρ and Rs factor. The 7S and 12S transcripts are mRNAs, which were initiated from PR and PL promotors of λ phage genome. When ppGpp was added, the amounts of 7S and 12S RNA were increased, but that of 26S RNA was not significantly affected. By DNA-RNA hybridization analysis, 26S RNA contained mostly the 30S precursor rRNA species. (4) Fractionation of the S100 fraction was accomplished by two sequential chromatography and by gel filtration. Each fraction was added to the purified RNA polymerase-λrifd18 DNA system, and the amount of rRNA synthesized was determined by DNA-RNA hybridization. Addition of the purified S factor stimulated synthesis of rRNA, and the transcription is susceptible to ppGpp inhibition.
It is expected that the S factor detected in vitro plays a physiological role as a positive effector controlling the in vivo transcription of rRNA.View full abstractDownload PDF (5525K) -
Toshibumi TSUZUKI, Yoshiaki ANDO1982 Volume 37 Issue 5 Pages 875-881
Published: September 25, 1982
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSStudies were undertaken in an attempt to identify the active sites of exchangeable cations within the spore of Clostridium perfringens type A NCTC 8238. Spores were converted by the base exchange technique to various ionic forms. Ca- and Sr-spores as well as native spores were fractionated by the following three procedures; i) mechanical disruption, ii) extraction with DTT, and iii) extraction with EDTA. The Ca and Sr contents of the soluble fraction of the disrupted spores were much higher than those of the insoluble fraction. However, these results did not appear to reflect the precise location of in situ Ca and Sr, since the drastic disruption of spores would cause redistribution of such exchangeable cations. Although the major amounts of Ca and Sr in these spores were found in the lysozyme-soluble fractions after being extracted with DTT, the DTT extract of Sr-spores contained a significant amount of Sr and a reduced amount of Ca. Most of Sr in Sr-spores was extracted with EDTA. In addition, electron probe X-ray microanalysis of the intact Sr-spores before and after treatment with EDTA confirmed that Sr was located in the spore integuments, mostly in the coats and partially in the peripheral layers of the cortex.View full abstractDownload PDF (3507K)
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