Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 30, Issue 6
Displaying 1-5 of 5 articles from this issue
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  • Epidemiology, Biochemistry, and Genetics of Conjugative and Nonconjugative Plasmids in Bacteria
    Susumu MITSUHASHI, Hajime HASHIMOTO, Kenji HARADA
    1975 Volume 30 Issue 6 Pages 661-674
    Published: November 25, 1975
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.30.661
    JOURNAL FREE ACCESS
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  • Studies on an RNA Turnover Mutant of Escherichia coli
    Yoshinari OHNISHI
    1975 Volume 30 Issue 6 Pages 675-682
    Published: November 25, 1975
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.30.675
    JOURNAL FREE ACCESS
    Download PDF (911K)
  • Simplified Methods for Purification of Staphylococcal Enterotoxins A and C and Preparation of Anti-Enterotoxin Sera
    Kunihiro SHINAGAWA, Nobuharu KUNITA, Genji SAKAGUCHI
    1975 Volume 30 Issue 6 Pages 683-692
    Published: November 25, 1975
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.30.683
    JOURNAL FREE ACCESS
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    The staphylococcal enterotoxins are proteins produced by staphylococci in foods and culture media under certain conditions. Staphylococcal food poisoning is caused by ingestion of enterotoxin. Therefore, diagnosis of staphylococcal food poisoning must be made by detecting enterotoxin in the incriminated foodstuff or cultures of strains isolated from the foodstuff and patients. Specific, highly potent anti-enterotoxin sera are necessary for such examinations. The staphylococcal enterotoxins have been classified into five types, A to E, on the basis of antigenicity.
    The methods for purification of enterotoxins reported by many invesigators are rather complicated and different from one type to another. To prepare anti-enterotoxin serum, a large amount (a few to tens of milligrams) of enterotoxin used to be divided into several doses and injected into a rabbit.
    The authors purified enterotoxins A and C by the same simplified method consisting of successive chromatography on CM-Sephadex and DEAE-Sephadex and gel filtration on Sephadex G-75. Each rabbit was injected subcutaneously twice with 10-μg doses of purified enterotoxin A or C to prepare anti-enterotoxin serum. The resulting anti-enterotoxin sera were highly specific with potencies high enough to be used for detection and identification of enterotoxins.
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  • Kidney Lodgments with Compact Type Staphylococcal Strains in Mice
    Munetaka KUNO, Masami TAKAHASHI, Kosaku YOSHIDA
    1975 Volume 30 Issue 6 Pages 693-697
    Published: November 25, 1975
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.30.693
    JOURNAL FREE ACCESS
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    Kidney lodgments were demonstrated in mice injected intravenously with compact type variants of Staphylococcus epidermidis strains. When mice were injected intravenously with the diffuse type variants of these strains, staphylococci disappeared from blood, liver, spleen, and kidney within 3 days after injection. A similar relation was observed between the compact and diffuse type variants of the Smith strain of S. aureus, although survival of the diffuse organisms in blood, liver, and spleen was significantly prolonged and a visible renal abscess was shown in one or two of the ten kidneys examined.
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  • [title in Japanese]
    1975 Volume 30 Issue 6 Pages 699-732
    Published: November 25, 1975
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.30.699
    JOURNAL FREE ACCESS
    Download PDF (5530K)
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