Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
Search
Advanced search
OR
Browse
Search
Volume 30, Issue 4
Displaying 1-4 of 4 articles from this issue
Show all abstracts
    • |<
    • <
    • 1
    • >
    • >|
  • Bacteriology and Ecology of Yersinia enterocolitica and Yersinia pseudotuberculosis, and their Infections
    Hiroshi ZEN-YOJI
    1975 Volume 30 Issue 4 Pages 571-581
    Published: July 25, 1975
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.30.571
    JOURNAL FREE ACCESS
    Download PDF (1280K)
  • Ability of Antigenic Substances from Culture Filtrates of Aspergillus fumigatus and Candida albicans to Elicit lmmunological Reactions in Guinea Pigs Sensitized with Homologous Fungal Cells
    Masuko SUZUKI, Yoshiro HAYASHI
    1975 Volume 30 Issue 4 Pages 583-588
    Published: July 25, 1975
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.30.583
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The polysaccharides and polysaccharide-protein complexes obtained from culture filtrates of Aspergillus fumigatus and Candida albicans were investigated for ability to elicit immunological reactions in guinea pigs sensitized with the whole homologous fungal cells.
    1) The polysaccharide-protein complexes of both fungal species elicited the skin reaction in the guinea pigs sensitized with homologous fungal cells, but no simple polysaccharides elicited any reaction.
    2) All the polysaccharide-protein complexes and polysaccharides were found to be active in the macrophage migration-inhibition test using splenic cells harvested from guinea pigs sensitized with the whole homologous fungal cells. Additionally, the macrophage migration-inhibition by the polysaccharide of C. albicans was inhibited significantly in the presence of such oligosaccharide as corresponding to the antigenic determinants of the respective polysaccharide.
    3) When modified chemically by partial acid-degradation and periodate oxidation, these polysaccharides caused complete lass of the activity in the macrophage migration-inhibition test.
    View full abstract
    Download PDF (705K)
  • Preservation of the Gonococcus by Gelatin-Disk Method
    Shiro YAMAI, Mariko NISHIMOTO, Takayasu NIKKAWA, Yasushi OBARA, Yasush ...
    1975 Volume 30 Issue 4 Pages 589-593
    Published: July 25, 1975
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.30.589
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Preservation of the gonococcus is one of the troublesome problems in the study of gonorrhoea because of the delicate viability of the organism in vitro. In order to solve the problem, an attempt was made to apply the gelatin-disk method reported by Stamp to preservation of the organism.
    An overnight culture of the organism on GC agar was suspended in a 3% skim milk solution containing 5% glucose. One volume of the resultant suspension was mixed with one volume of a warmed 20% gelatin solution and one-fifth volume of a 2.5% L-ascorbic acid solution. Drops (about 0.03ml each) of the mixture were placed on a piece of paraffin-soaked filter paper and dried over P2O5 and silica gel under a reduced pressure of 20mm of mercury. Dried gelatin disks were stripped from the piece of filter paper and put into a test tube containing silica gel. Then the tube was sealed up.
    The dried gelatin disks thus obtained from 12 strains of Neisseria gonorrhoeae were kept at -20C and tested monthly for viability over one year.
    All the cultures tested well retained their viability. The number of viable cells decreased gradually to reach about one-tenth of the initial level after one-year storage.
    These cultures also well maintained colony form, hemagglutinability to rabbit and guinea pig red blood cells, and susceptibility to streptomycin for at least one year.
    View full abstract
    Download PDF (589K)
  • [title in Japanese]
    1975 Volume 30 Issue 4 Pages 595-604
    Published: July 25, 1975
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.30.595
    JOURNAL FREE ACCESS
    Download PDF (1409K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
 Top