Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 29, Issue 3
Displaying 1-8 of 8 articles from this issue
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  • [title in Japanese]
    [in Japanese]
    1974 Volume 29 Issue 3 Pages 495-506
    Published: May 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.495
    JOURNAL FREE ACCESS
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  • Influence of Medium Components on the Colonial Morphology of Anaerobic Bacterial Strains in Soft-Agar Medium
    Kiyoko NAKANISHI, Yukiko MORIWAKE
    1974 Volume 29 Issue 3 Pages 507-515
    Published: May 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.507
    JOURNAL FREE ACCESS
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    Nine strains of 4 anaerobic bacterial species were grown in soft-agar medium to observe themorphology of their colonies. The colonies were classified by the feature of growth type as follows: compact, diffuse, feathery, and tailed. On the basis of the appearance of overall growths, they weredesignated as cloud-like, coral-like, curtain-like, or cotton-like. In order to elucidate the relationshipbetween the colonial molphology and the composition of a medium, the concentrations of Tryptone (Difco), Soytone (Difco), agar, glucose, and cystine were changed in the basal medium. Resultsshowed that the Tryptone, Soytone, and glucose contents mainly influenced the growth types of colonies, and that the agar and cystine concentrations were closely related to the colonial form.
    The specific characteristics of the colonial form were, therefore, found to have been influenced byboth growth and physical factors. The typical features of the colonial morphology and overall growthsof the anaerobic bacterial strains in soft-agar medium were reproducible in the medium with the followingcomposition: 1.5 to 3 per cent Tryptone, 0.3 to 2.0 per cent Soytone, 0.07 to 0.08 per cent agar, 0.2to 0.4 per cent glucose, and 0.08 to 0.12 per cent cystine.
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  • Cultivation of Mycobacterium lepraemurium in a Cell-Free Liquid Medium
    Masahiro NAKAMURA
    1974 Volume 29 Issue 3 Pages 517-525
    Published: May 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.517
    JOURNAL FREE ACCESS
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    A cell-free liquid medium, which was referred to as NC-5, was established for cultivation of Mycobacterium lepraemurium. It was composed of the basal medium and some additives. The formerwas the Kirchner medium enriched with glucose, sodium pyruvate, and calcium pantothenate, andthe latter were goat serum, α-ketoglutaric acid, cytochrome C, hemin, and l-cystein hydrochloride. Allthe additives had to be added aseptically to the basal medium.
    When bacilli were cultivated in the NC-5 medium at 30 C, bacterial cells began to be elongatedgradually at 4-7 days of cultivation and started to multiply at 2 weeks. Finally, the growth of bacillicould be recognized macroscopically as a turbid mass. A possibility that the growth obtained in NC-5 might be due to the multiplication of other acid-fast bacilli which might have contaminated thestarting material was excluded, as no growth of bacilli was observed in the Kirchner medium, whichis the most suitable for the growth of most acid-fast bacilli.
    The growth of Mycobacterium lepraemurium reached a maximum in the NC-5 medium at 8 weeksof cultivation. The increase rate was about 100 or 1, 000 times. It is of great interest to note thatsmaller the inoculum size, the high was the growth rate. The possible generation time calculated fromthe bacillary count might be approximately from 8 to 14 days. The growth was inhibited by additionof isoniazid, streptomycin, and mitomycin C, but not by penicillin.
    In a preliminary trial, no bacilli multiplied in the second generation when the bacilli grown inthe NC-5 medium had been inoculated into a freshly prepared NC-5 medium. An abundant multiplicationof bacilli was observed however, when bacilli were transferred from a smear on the slide tothe NC-5 medium at definite intervals. Finally, it was indicated in animal experiments that thebacilli cultivated in the NC-5 medium for 223 days kept their pathogenic activity. On the contrary, no pathogenicity was observed in the bacilli cultivated in the Kirchner medium under the same conditions.
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  • [title in Japanese]
    1974 Volume 29 Issue 3 Pages 527-544
    Published: May 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.527
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1974 Volume 29 Issue 3 Pages 545-562
    Published: May 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.545
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1974 Volume 29 Issue 3 Pages 563-585
    Published: May 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.563
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1974 Volume 29 Issue 3 Pages 586-595
    Published: May 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.586
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1974 Volume 29 Issue 3 Pages 596-632
    Published: May 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.596
    JOURNAL FREE ACCESS
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