Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 31, Issue 4
Displaying 1-4 of 4 articles from this issue
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  • [title in Japanese]
    [in Japanese]
    1976 Volume 31 Issue 4 Pages 535-555
    Published: July 25, 1976
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.31.535
    JOURNAL FREE ACCESS
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  • Effect of Sonicated Schizophyllan on the Formation of Immunoglobulins
    Motoji SUZUKI, Nobuhiko KOMATSU
    1976 Volume 31 Issue 4 Pages 557-564
    Published: July 25, 1976
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.31.557
    JOURNAL FREE ACCESS
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    The plaque technique of Cunningham and Szenberg was used to demonstrate the adjuvant effect of sonicated schizophyllan (SPG-S), a glucan produced by Schizophyllum commune, on antibody formation.
    1) The pretreatment of mice with SPG-S, as well as the original schizophyllan, was proved to increase the number of plaque-forming cells of the spleen after immunization with sheep red blood cells (SRBC). The highest adjuvant effect of SPG-S was obtained by a dose of 2.5-5.0 mg/kg.
    2) The administration of SPG-S caused a significant increase in both anti-SRBC IgM and IgG antibody-producing cells in the mouse spleen after the immunization. The number of these cells reached a peak 4 and 6 days, respectively, after the injection of antigen.
    3) SPG-S showed a remarkable adjuvant effect when injected by the intramuscular, intraperitoneal, and intravenous route, but not by the subcutaneous route.
    4) Examination of time intervals between SPG-S and antigen injections revealed that SPG-S presented a most conspicuous adjuvant effect when injected 24 hours before the administration of antigen.
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  • Effects of Endotoxins Hydrolyzed with Acid or Alkali on Bone Marrow Reactions
    Michimasa HIRATA
    1976 Volume 31 Issue 4 Pages 565-570
    Published: July 25, 1976
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.31.565
    JOURNAL FREE ACCESS
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    Hemorrhage and necrosis are observed in the mouse bone marrow after administration with endotoxin (bone marrow reactions). In the present paper, studies were made on effect of acid or alkaline hydrolysis of Salmonella typhimurium endotoxins on their activity in the bone marrow reactions. No reaction was shown by acetic hapten (polysaccharide moiety) isolated from S endotoxin by hydrolysis with 0.1 N acetic acid at 100 C for 90 min.
    The lipid A fraction obtained from S endotoxin by either acetic acid hydrolysis. or hydrochloric acid hydrolysis had no activity. These results seemed to be mainly dependent on the poor solubility of lipid A in the solvent used. S endotoxin was inactivated more rapidly than R endotoxin by hydrolysis with 0.1 N NaOH at 30 C, but was almost at the same time as R endotoxin by that at 56C. When investigated by the reactions, the endotoxins decreased in necrotic activity by acid or alkaline hydrolysis. S endotoxin was reduced more rapidly in necrotic activity than R endotoxin by alkaline hydrolysis at 30C.
    The physicochemical factors of the endotoxins which affected the manifestation of endotoxic activities were discussed.
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  • [title in Japanese]
    1976 Volume 31 Issue 4 Pages 571-581
    Published: July 25, 1976
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.31.571
    JOURNAL FREE ACCESS
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