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Volume 19, Issue 4
Displaying 1-4 of 4 articles from this issue
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V. Change of Serological Properties of Immunospecific and Non-specific Substance by Enzymatic DigestionTsuyoshi BABA1964 Volume 19 Issue 4 Pages 121-124
Published: April 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESS1) Immunospecific substance, made mainly of protein, is hydrolysed in proteolytic enzyme and its antigenicity is reduced by about 1/4.
2) Non-specific substance, made mainly of a polysaccharide is not entirely hydrolysed by the known carbohydrase, and no reduction of antigenicity was seen.
3) In the hepatic-splenic homogenate of normal rabbits, immunospecific substance has its antigenicity reduced by about 1/4. No such reduction of antigenicity, however, is noted with non-specific substance.View full abstractDownload PDF (577K) -
On the Antigenic Substances in Culture Media of FungiOsamu SAKAGUCHI, Shigeo SUZUKI, Masuko SUZUKI, Katsushi YOKOTA1964 Volume 19 Issue 4 Pages 125-130
Published: April 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSThe MeOH- and Sephadex-fractionation were done for the isolation of immunological active substances in culture media of three species fungi Aspergillus fumigatus, Rhizopus arrhizus and Trichophyton rubrum.
Chemical and serological properties of the extracellular antigenic substances produced by fungi were examined, and the following results were obtained.
The component sugars of the polysaccharides were identified as follows. Polysaccharide of Asp. fumigatus was composed of galactose, mannose and glucose as similar to Tri. rubrum, but that of Rhizopus arrhizus was shown to possess mannose and galactose.
Hexosamine amount was indicated in a range of 0.5-1.5% with Elson-Morgan method.
A large quantity of the yields of MeOH-fractionation were gained in concentration of 75-85% MeOH.
Each fractions containing glycoprotein were indicated similarity of paper electrophoretic pattern at nearly starting point.
At the serological activity of precipitin reaction, Asp. fumigatus and Tri. rubrum have been shown a largest activity when experiments were tested with 25-75% MeOH-precipited fraction. It was different from the fact that culture filtrate of Rhi. arrhizus have been shown scarcely activity of precipitin reaction.View full abstractDownload PDF (849K) -
IV. On the Clinical and Pathological Observations in the Monkies Introduced with Dysentery Bacilli into the Stomach After the Starvation for 2 to 3 daysNagao HAYASHI, Shigeo IWAHARA1964 Volume 19 Issue 4 Pages 131-134
Published: April 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSThis experiment was made for the purpose of comparing with administrations of report I (on the clinical and pathological observations in the monkies introduced with dysentery bacilli into the stomach and the intestinal lumen), III (on the clinical and pathological observtions in the monkies introduced with dysentery bacilli into the stomach after pretreatment: oral administrations of sonicate of dysentery bacilli or chloramphenicol) and IV (on the clinical and pathological observations in the monkies introduced with dysentery bacilli into the stomach after the starvation for 2 to 3 days).
6 monkies (M.irus) weighing 2 to 3kg were used in this work.
Those which were starved were deprived of food for 2 to 3 days before oral administration of living cultures (10×1010 to 17×1010 cells).
The results were as follows:
In 5 of 6 animals challenged, symptoms of acute bacillary dysentery were observed and the symptoms were closely resembled to those of acute bacillary dysentery which are seen in human beings.
They produced clinical and pathological observations similar to those described in the reports I and III.
The organisms were found throughout the length of the intestinal tract.
Gastritis and colitis with the hemorrhage were recognized.View full abstractDownload PDF (595K) -
1964 Volume 19 Issue 4 Pages 135-144
Published: April 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (2090K)
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