Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 29, Issue 2
Displaying 1-11 of 11 articles from this issue
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  • [title in Japanese]
    [in Japanese]
    1974 Volume 29 Issue 2 Pages 313-320
    Published: March 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.313
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese]
    1974 Volume 29 Issue 2 Pages 321-322
    Published: March 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.321
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese], [in Japanese]
    1974 Volume 29 Issue 2 Pages 323-328
    Published: March 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.323
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese]
    1974 Volume 29 Issue 2 Pages 329-335
    Published: March 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.329
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese]
    1974 Volume 29 Issue 2 Pages 336-344
    Published: March 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.336
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese]
    1974 Volume 29 Issue 2 Pages 345-353
    Published: March 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.345
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese]
    1974 Volume 29 Issue 2 Pages 354-367
    Published: March 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.354
    JOURNAL FREE ACCESS
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  • Immunochemical Studies on Chromoblastomycosis
    I. Isolation and Purification of Antigenic polysaccharides from Mycelia and Culture Filtrates of Hormodendrum pedrosoi, H. compactum and H. dermatitidis
    Noriyuki TAKEDA, Shigeo SUZUKI
    1974 Volume 29 Issue 2 Pages 369-378
    Published: March 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.369
    JOURNAL FREE ACCESS
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    Three representative strains of the genusHormodendrum, H. pedrosoiIFO 6071, H. compactumIFO 6726, andH. dermatitidisIFO 6421, were grown in Sabouraud's medium containing yeast extract to harvest the respective homogeneous cells of mycelial phase. The acetone-dried cells of each strain were extracted with 1/15 M phosphate, pH 7.0, at 135 C. Then crude polysaccharides were obtained by the ethanol precipitation.
    The separation of the crude polysaccharides from the culture filtrates of these strains was made by the ethanol precipitation after successive processes of dialysis and concentration. Fractionation of the crude polysaccharides by gel filtration on a Sephadex G-75 column gave each galactomannan and glucan. The former polysaccharides were further fractionated into 1 major and 2 minor subfractions by DEAE-Sephadex chromatography.
    All the major fractions were shown to be homogeneous by ultracentrifugal analysis. They had strong precipitating activities against homologous anti-whole cell rabbit sera.
    In the agar-gel double diffusion reaction, 6 galactomannans isolated from each strain gave a single precipitin arc which fused completely with adjacent ones. In the precipitin reaction, these polysaccharides were also highly cross-reactive to heterologous anti-Hormodendrumsera. On the other hand, no serological activity could be observed on any of the glucan fractions purified by a procedure similar to that used for the purification of the galactomannans.
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  • Mechanism of Action of Clotrimazole
    II. Effects on the Cell Membrane of Candida albicans
    Hideyo YAMAGUCHI, Kazuo IWATA
    1974 Volume 29 Issue 2 Pages 379-385
    Published: March 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.379
    JOURNAL FREE ACCESS
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    Clotrimazole had the enhancing effects on K-efflux and leakage of intracellular phosphorous compounds ofCandida albicansstrain 6713 at a fungicidal level (above 20μg/ml). Conversely, at fungistatic levels (2.5-10μg/ml), the drug was found to prevent these intracellular substances from leaking.
    The protoplasts of this organism were shrunken at a fungicidal concentration (30μg/ml) and then suffered from a conspicuous breakdown.
    When added to a cell suspension at 30μg/ml, the drug was rapidly incorporated in cells, reaching a level 130 times higher than that in the bathing medium after 30 minutes. After that, a large portion of the incorporated drug leaked out of the cell.
    When incorporated, the drug was adsorbed to the cell membrane and the greater part of the incorporated drug was combined with the cell membrane structures, including the cytoplasmic membrane.
    Clotrimazole enhanced remarkably the permeability of “liposome”, artificial phospholipid globules as a model of the lipid layer of the cell membrane, regardless of the existence of sterols in the lipid membrane.
    These results, together with those presented in the preceding paper, would lend further support to the authors' view that clotrimazole acts primarily on the cell membrane of sensitive organisms by damaging the permeability barrier through its specific ability to bind with a target cell structure, which is presumably composed of phospholipids.
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  • Distribution of Motile Streptococci in Feces of Man and Animals and in River and Sea Water
    Hideo HASHIMOTO, Reiko NOBORISAKA, Ryo YANAGAWA
    1974 Volume 29 Issue 2 Pages 387-393
    Published: March 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.387
    JOURNAL FREE ACCESS
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    Attempts were made to isolate motile streptococci from fecal samples of man and animals, as well as from samples of river and sea water. A wide distribution of motile streptococci in these samples was found. The results obtained, including those of electron microscopic observation of flagella of these organisms, are summarized as follows.
    1. The detection rate of motile streptococci from feces was 26%(13/50) in man, 46.6%(14/30) in chickens, 50%(5/10) in turkeys, 65%(13/20) in sheep, 20%(4/20) in cattle, 3.3%(1/30) in dogs, and 72%(18/25) in mice, and that from river water was 14 %(7/50) and that from sea water 50%(6/12).
    2. Of the 104 isolates of motile streptococci, 92 were Streptococcus faecium, 5 atypical enterococci, 4S. bovis, and 3 unclassified. Accordingly, most of the isolates belonged to enterococci, and a few were different from enterococci.
    3. Electron microscopic observation of motile streptococci revealed that these organisms contained a high percentage of flagellated cells when they were cultivated at low temperature; namely, 49% at 25 C, 14.7% at 30 C and only 1.3% at 37C.
    4. The number of flagella per cell was usually 1. In some exceptional cases it was 2 or 3. The flagella ranged from 1 to more than 10μin length and approximately 7.5 mp in diameter. Coils of flagella of regular wavelength and amplitude were occasionally found.
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  • [title in Japanese]
    1974 Volume 29 Issue 2 Pages 395-405
    Published: March 25, 1974
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.3412/jsb.29.395
    JOURNAL FREE ACCESS
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